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Comparing Estimates of Seed Viability in Three Foxtail (Setaria) Species Using the Imbibed Seed Crush Test with and without Additional Tetrazolium Testing

Published online by Cambridge University Press:  20 January 2017

Jaclyn K. Borza ,
Paula R. Westerman  and
Matt Liebman
Jaclyn K. Borza*
Affiliation:
Department of Agronomy, Agronomy Hall, Iowa State University, Ames, IA 50011
Paula R. Westerman
Affiliation:
Department of Agronomy, Agronomy Hall, Iowa State University, Ames, IA 50011
Matt Liebman
Affiliation:
Department of Agronomy, Agronomy Hall, Iowa State University, Ames, IA 50011
*
Corresponding author's E-mail: jkborza@iastate.edu
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Abstract

Estimates of seed viability using the imbibed seed crush test, a method performed by applying pressure to imbibed seeds, were compared with estimates obtained from using the imbibed seed crush test supplemented with tetrazolium staining. The seeds of three weed species, giant foxtail, green foxtail, and yellow foxtail, were collected from three different crops and tested by each method. The results from the two approaches were strongly and significantly correlated. The imbibed seed crush test requires considerably less skill and time to perform and is a reasonable alternative to tetrazolium staining to test the seed viability of newly produced foxtail seeds.

Keywords

Tetrazolium, 2,3,5 triphenyl tetrazolium chloridegiant foxtail, Setaria faberi Herrm. SETFAgreen foxtail, Setaria viridis (L.) Beauv. SETVIyellow foxtail, Setaria glauca (L.) Beauv. SETLUCRimbibed seed crush testTZtetrazolium test
Type
Notes
Information
Weed Technology , Volume 21 , Issue 2 , June 2007 , pp. 518 - 522
Copyright
Copyright © Weed Science Society of America 

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References

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