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Published online by Cambridge University Press: 13 October 2025
Field experiments were conducted near Beresford and South Shore, South Dakota, in 2023 and 2024 to determine weed control and soybean yield with 2,4-D and glufosinate applied alone, mixed, and sequentially. Sequential applications were made 12 d after the initial application. 2,4-D plus glufosinate additively controlled and reduced the height of all tested weed species. Sequential applications increased common lambsquarters, waterhemp, redroot pigweed, and velvetleaf control compared with 2,4-D, glufosinate, or 2,4-D plus glufosinate applied alone. The herbicide order in the sequential application did not influence broadleaf weed control. Yellow foxtail control was greater with sequential applications of glufosinate. Soybean yield at Beresford was similar across all treatments. Yields were generally greater with sequential herbicide applications, where glufosinate was applied initially at South Shore. The experiment results suggest that weed control and soybean yield are greater with 2,4-D plus glufosinate or sequential application treatments utilizing 2,4-D and glufosinate.