Water is the primary carrier for herbicide application, and carrier-water–related factors can influence herbicide performance. In a greenhouse study, premixed formulation of glyphosate plus dicamba was mixed in deionized (DI) water at 5, 18, 31, 44, or 57 C and applied immediately. In a companion study, glyphosate and dicamba formulation was mixed in DI water at temperatures of 5, 22, 39, or 56 C and sprayed after the herbicide solution was left at the respective temperatures for 0, 6, or 24 h. In both studies, glyphosate plus dicamba was applied at 0.275 plus 0.137 kg ae ha−1 (low rate), and 0.55 plus 0.275 kg ha−1 (high rate), respectively, to giant ragweed, horseweed, Palmer amaranth, and pitted morningglory. Glyphosate plus dicamba applied at a low rate with solution temperature of 31 C provided 14% and 26% greater control of giant ragweed and pitted morningglory, respectively, compared to application at solution temperature of 5 C. At both rates of glyphosate and dicamba formulation, giant ragweed and pitted morningglory control was 15% or greater at solution temperature of 44 C compared to 5 C. Weed control was not affected with premixture of glyphosate and dicamba applied ≤ 24 h after mixing herbicide. When considering solution temperature, glyphosate and dicamba applied at low rate provided 13 and 6% greater control of Palmer amaranth and pitted morningglory, respectively, with solution temperature of 22 C compared to 5 C. Similarly, giant ragweed control was 8% greater with solution temperature of 39 C compared to 5 C. Glyphosate and dicamba applied at high rate provided 8% greater control of giant ragweed at solution temperature of 22 or 39 C compared to 5 C. Therefore, activity of premixed glyphosate and dicamba could be reduced with spray solution at lower temperature; however, the result is dependent on weed species.