Hostname: page-component-5db58dd55d-688nx Total loading time: 0 Render date: 2026-07-08T07:03:33.682Z Has data issue: false hasContentIssue false
  • English
  • Français

Ingestion of onion soup high in quercetin inhibits platelet aggregation and essential components of the collagen-stimulated platelet activation pathway in man: a pilot study

Published online by Cambridge University Press:  19 February 2008

Gary P. Hubbard ,
Siegfried Wolffram ,
Ric de Vos ,
Arnaud Bovy ,
Jonathan M. Gibbins  and
Julie A. Lovegrove
Gary P. Hubbard
Affiliation:
School of Food Biosciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6AL, UK School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6AL, UK
Siegfried Wolffram
Affiliation:
Institute of Animal Nutrition, Physiology and Metabolism, University of Kiel, D-24098 Kiel, Germany
Ric de Vos
Affiliation:
Plant Research International, 6700 AA Wageningen, The Netherlands
Arnaud Bovy
Affiliation:
Plant Research International, 6700 AA Wageningen, The Netherlands
Jonathan M. Gibbins
Affiliation:
School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6AL, UK
Julie A. Lovegrove*
Affiliation:
School of Food Biosciences, University of Reading, Whiteknights, Reading, Berkshire RG6 6AL, UK
*
*Corresponding author: Dr J. A. Lovegrove, fax +44 (0) 118 931 0080, email j.a.lovegrove@reading.ac.uk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the 'Save PDF' action button.

Epidemiological data suggest that those who consume a diet rich in quercetin-containing foods may have a reduced risk of CVD. Furthermore, in vitro and ex vivo studies have observed the inhibition of collagen-induced platelet activation by quercetin. The aim of the present study was to investigate the possible inhibitory effects of quercetin ingestion from a dietary source on collagen-stimulated platelet aggregation and signalling. A double-blind randomised cross-over pilot study was undertaken. Subjects ingested a soup containing either a high or a low amount of quercetin. Plasma quercetin concentrations and platelet aggregation and signalling were assessed after soup ingestion. The high-quercetin soup contained 69mg total quercetin compared with the low-quercetin soup containing 5mg total quercetin. Plasma quercetin concentrations were significantly higher after high-quercetin soup ingestion than after low-quercetin soup ingestion and peaked at 2·59 (sem 0·42) μmo/. Collagen-stimulated (0·5μ/l) platelet aggregation was inhibited after ingestion of the high-quercetin soup in a time-dependent manner. Collagen-stimulated tyrosine phosphorylation of a key component of the collagen-signalling pathway via glycoprotein VI, Syk, was significantly inhibited by ingestion of the high-quercetin soup. The inhibition of Syk tyrosine phosphorylation was correlated with the area under the curve for the high-quercetin plasma profile. In conclusion, the ingestion of quercetin from a dietary source of onion soup could inhibit some aspects of collagen-stimulated platelet aggregation and signalling ex vivo. This further substantiates the epidemiological data suggesting that those who preferentially consume high amounts of quercetin-containing foods have a reduced risk of thrombosis and potential CVD risk.

Keywords

QuercetinPlateletsglycoprotein VIPhytochemicalsCVDSignalling

Information

Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 3 , September 2006 , pp. 482 - 488
Copyright
Copyright © The Nutrition Society 2006

References

Ader, P, Wessmann, A & Wolffram, S (2000) Bioavailability and metabolism of the flavonol quercetin in the pig. J Free Radic Biol Med 28, 1056–1067.CrossRefGoogle ScholarPubMed
Aziz, AA, Edwards, CA, Lean, MEJ & Crozier, A (1998) Absorption and excretion of conjugated flavonols, including quercetin-4′-O-beta-glucoside and isorhamnetin-4′-O-beta-glucoside by human volunteers after the consumption of onions. Free Radic Res 29, 257–269.CrossRefGoogle ScholarPubMed
Beretz, A, Cazenave, JP & Anton, R (1982) Inhibition of aggregation and secretion of human-platelets by quercetin and other flavonoids – structure activity relationships. Agents Actions 12, 382–387.CrossRefGoogle ScholarPubMed
Bovy, AG, de Vos, CHR, Kemper, M, Almenar, Pertejo M, Muir, SR, Collins, GJ, Robinson, S, Verhoeyen, ME, Hughes, SG & van Tunen, AJ (2002) High-flavonol tomatoes through heterologous expression of the maize transcription factor genes LC and C1. lant Cell 14, 2509–2526.Google ScholarPubMed
Cicmil, M, Thomas, JM, Leduc, M, Bon, C & Gibbins, JM (2002) PECAM-1 signalling inhibits the activation of human platelets. Blood 99, 137–144.CrossRefGoogle ScholarPubMed
Conquer, JA, Maiani, G, Azzini, E, Raguzzini, A & Holub, BJ (1998) Supplementation with quercetin markedly increases plasma quercetin concentration without effect on selected risk factors for heart disease in healthy subjects. J Nutr 128, 593–597.CrossRefGoogle ScholarPubMed
de Pascual-Teresa, S, Johnston, KL, DuPont, MS, O'Leary, KA, Needs, PW, Morgan, LM, Clifford, MN, Bao, YP & Williamson, G (2004) Quercetin metabolites downregulate cyclooxygenase-2 transcription in human lymphocytes ex vivo but not in vivo. J Nutr 134, 552–557.Google Scholar
Duffy, SJ, Vita, JA, Holbrook, M, Swerdloff, PL & Keaney, JF (2001) Effect of acute and chronic tea consumption on platelet aggregation in patients with coronary artery disease. Arterioscler Thromb Vasc Biol 21, 1084–1089.CrossRefGoogle ScholarPubMed
Femia, AP, Caderni, G, Ianni, M, Salvadori, M, Schijlen, E, Collins, G, Bovy, A & Dolora, P (2003) Effects of diets fortified with tomatoes or onions with variable quercetin-glycoside content on azoxymethane-induced aberrant crypt foci in the colon of rats. Eur J Nutr 42, 346–352.CrossRefGoogle ScholarPubMed
Formica, JV & Regelson, W (1995) Review of the biology of quercetin and related bioflavonoids. Food Chem Toxicol 33, 1061–1080.CrossRefGoogle ScholarPubMed
Gibbins, JM, Okuma, M, Farndale, R, Barnes, M & Watson, SP (1997) Glycoprotein VI is the collagen receptor in platelets which underlies tyrosine phosphorylation of the Fc receptor gamma-chain. FEBS Letters 413, 255–259.CrossRefGoogle ScholarPubMed
Gosnay, S, Bishop, J, New, S, Bishop, S, Catterick, J & Clifford, M (2002) Estimation of the mean intakes of 14 classes of dietary phenols in a population of young British women aged 20–30 years. Free Radic Res 36, 115–116.Google Scholar
Hertog, MGL, Feskens, EJM, Hollman, PCH, Katan, MB & Kromhout, D (1993) Dietary antioxidant flavonoids and risk of coronary heart-disease – the Zutphen Elderly study. Lancet 342, 1007–1011.CrossRefGoogle ScholarPubMed
Hertog, MGL, Hollman, PCH & Katan, MB (1992) Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agric Food Chem 40, 2379–2383.CrossRefGoogle Scholar
Hertog, MG, Kromhout, D, Aravanis, C, Blackburn, H, Buzina, R, Fidanza, F, Giampaoli, S, Jansen, A, Menotti, A & Nedeljkovic, S (1995) Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Arch Int Med 155, 381–386.CrossRefGoogle ScholarPubMed
Hertog, MGL, Sweetman, PM, Fehily, AM, Elwood, PC & Kromhout, D (1997) Antioxidant flavonols and ischemic heart disease in a Welsh population of men: the Caerphilly Study. Am J Clin Nutr 65, 1489–1494.CrossRefGoogle Scholar
Hodgson, JM, Puddey, IB, Burke, V, Beilin, LJ, Mori, TA & Chan, SY (2002) Acute effects of ingestion of black tea on postprandial platelet aggregation in human subjects. Br J Nutr 87, 141–145.CrossRefGoogle ScholarPubMed
Hollman, PCH, Bijsman, M, van Gameren, Y, Cnossen, EPJ, de Vries, JHM & Katan, MB (1999) The sugar moiety is a major determinant of the absorption of dietary flavonoid glycosides in man. Free Radic Res 31, 569–573.CrossRefGoogle Scholar
Hollman, PCH, Hertog, MGL & Katan, MB (1996) Role of dietary flavonoids in protection against cancer and coronary heart disease. Biochem Soc Trans 24, 785–789.CrossRefGoogle ScholarPubMed
Hubbard, GP, Stevens, JM, Cicmil, M, Sage, T, Jordan, PA, Williams, CM, Lovegrove, JA & Gibbins, JM (2003) Quercetin inhibits collagen-stimulated platelet activation through inhibition of multiple components, of the glycoprotein VI signaling pathway. J Thromb Haemost 1, 1079–1088.CrossRefGoogle ScholarPubMed
Hubbard, GP, Wolffram, S, Lovegrove, JA & Gibbins, JM (2004) Ingestion of quercetin inhibits platelet aggregation and essential components of the collagen-stimulated platelet activation pathway in humans. J Thromb Haemost 2, 2138–2145.CrossRefGoogle ScholarPubMed
Janssen, P, Mensink, RP, Cox, FJJ, Harryvan, JL, Hovenier, R, Hollman, PCH & Katan, MB (1998) Effects of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in vitro and a dietary supplement study. Am J Clin Nutr 67, 255–262.CrossRefGoogle Scholar
Keevil, JG, Osman, HE, Reed, JD & Folts, JD (2000) Grape juice, but not orange juice or grapefruit juice, inhibits human platelet aggregation. J Nutr 130, 53–56.CrossRefGoogle ScholarPubMed
Landolfi, R, Mower, RL & Steiner, M (1984) Modification of platelet-function and arachidonic-acid metabolism by bioflavonoids-structure activity relations. Biochem Pharmacol 33, 1525–1530.CrossRefGoogle ScholarPubMed
McAnlis, GT, McEneny, J, Pearce, J & Young, IS (1999) Absorption and antioxidant effects of quercetin from onions, in man. Eur J Clin Nutr 53, 92–96.CrossRefGoogle ScholarPubMed
Manach, C, Regerat, F, Texier, O, Agullo, G, Demigne, C & Remesy, C (1996) Bioavailability, metabolism and physiological impact of 4-oxo-flavonoids. Nutr Res 16, 517–544.CrossRefGoogle Scholar