The Imbrie-Kipp method of paleotemperature estimation is rigorously tested by comparing Atlantic temperature equations independently derived from the microfossils of three biotic groups: the Foraminifera, Coccolithophorida, and Radiolaria. This method consists of two steps: factor analysis of the modern sea-bed data of the individual groups which resolves discrete biogeographic assemblages and regression analysis of the modern assemblage data with observed sea-surface temperature data to obtain paleotemperature equations. Assemblage biogeography shows a simple subdivision into warm (low latitude) and cold (high latitude) for all biotic groups. Between biotic groups there is greater similarity among high-latitude assemblages than low-latitude ones. Correlating the assemblage data with observed sea-surface temperatures to produce temperature distribution patterns shows differences of less than 2°C in their optimum and critical temperatures. Regression analysis produced accurate temperature equations for each biotic group, all with standard errors of estimate of less than or equal to 2°C. Multiple correlation coefficients were all greater than 0.970. Applying these equations to two multiple biotic data sets (the modern and ice-age sea-bed data) and comparing their temperature estimates using the standard error pooled, shows over 87% concordancy for both data sets. Unlike the modern data, the discordancy among temperature estimates of the ice-age data shows a distinct geographic distribution; its cause is believed to be oceanographic, a difference in the water-mass structure between the modern and ice-age ocean.

The paleoceanographic–climatic record represented by deep-sea microfossils reflects conditions for only certain times of the year. Also, the relative abundances of microfossil species in deep-sea sediments do not usually reflect only one paleoceanographic variable, such as temperature. Rather, species distributions represent the integration of many factors that control biological production in the oceans. This influences the information on past climates that can be extracted from fossil material. The seasonal limitation is due to the cyclic nature of biological production in the open ocean. Case studies of the sediment record in the Atlantic for two species of planktonic Foraminifera, left-coiling Neogloboquadrina pachyderma (Ehrenberg) and Globigerinoides ruber (d'Orbigny), illustrate seasonal bias in environmental data reported by the relative abundances of species in deep-sea sediments. In addition, the study of G. ruber illustrates the operation of two oceanographic parameters in controlling a species distribution. These examples demonstrate that the environmental signal in the sediments is the result of the interplay of the ecological tolerance of the plankton species with seasonally variable biological and physical properties of the upper ocean.

Benthonic foraminiferal faunal and isotopic data from Champlain Sea sediments (approximately 12,500 to 10,000 yr B.P. in age) in two piston cores from Lake Champlain provide a detailed, apparently continuous record of the evolution of the Champlain Sea. Cassidulina reniforme and Islandiella helenae are the dominant benthonic foraminifera during the initial phase of the Champlain Sea, and are replaced by Elphidium excavatum forma clavatum and Protelphidium orbiculare as the dominant species during the remainder of the sea's history. The oxygen-isotopic data show a gradual decrease in δ18O between approximately 12,500 and 10,900 yr B.P., followed by a more rapid decrease during the interval 10,900 to 10,000 yr B.P. The δ13C data have a similar trend as δ18O, with generally decreasing values up the section. The isotopic and faunal data suggest that nearly marine conditions were present in the initial plase of the Champlain Sea, followed by gradually decreasing salinities and increasing temperatures as the sea evolved. The beginning of the rapid isotopic decrease at approximately 10,900 yr B.P. marks the onset of the largest environmental change in the history of the Champlain Sea, probably reflecting a major pulse of meltwater from the Laurentide Ice Sheet.

Pollen spectra from cores of organic spring deposits from the Transvaal provide evidence for the climatic evolution of the province during the last 35,000 yr B.P. or more. The past climatic phases are derived from palynological reconstructions of past vegetation types by comparison of fossil pollen data with modern surface pollen spectra from various localities. Evidence is provided for an early moist, cool phase with relatively mesic bushveld and expanded montane forest in the central Transvaal, followed by a drier period with drier bushveld which probably lasted until approximately 25,000 yr B.P. During the next phase, which at the latest ended about 11,000 yr B.P., the temperatures were probably 5°–6°C cooler than at present. At that time bushveld vegetation in the central Transvaal was replaced by open grassland with macchia elements. Climatic amelioration came and semiarid savanna returned to the plains, at first gradually and then developing into a warm Kalahari thornveld-type vegetation. After 6000 yr B.P. it apparently became slightly wetter and a more broad-leafed bushveld developed. About 4000 yr B.P. it again became cooler and slightly wetter and the bushveld vegetation on the central and northern plains was comparable to present open upland types. After 2000 yr B.P. conditions gradually became warner until about 1000 yr B.P., when the modern climate of the central Transvaal bushveld originated.

Ennadai Lake, in the forest-tundra ecotonal region of Keewatin, Northwest Territories, Canada, has been the subject of several paleoecological investigations (palynology, plant macrofossils, fossil soils). This study concerns Holocene insect fossils at Ennadai, a new approach in a region shown to be sensitive to climatic change. The Ennadai I site yielded 53 taxa, representing 13 families of Coleoptera and 7 families of other insects and arachnids, including abundant ants. These fossils range in age from about 6300 to 630 yr B.P. The Ennadai II site produced fossils of 58 taxa, including 13 beetle families and 15 families of other arthropods, ranging in age from 4700 to 870 yr B.P. The insect evidence suggests the presence of trees in the Ennadai region from 6000 to 2200 yr B.P. A conifer pollen decline from 4800 to 4500 yr B.P. at Ennadai has previously been interpreted as an opening up or retreat of forest in response to climatic cooling, but the insect fossils reveal the continued presence of trees during this interval. Both insect assemblages suggest trends of forest retreat and tundra expansion between about 2200 and 1500 yr B.P., presumably due to climatic cooling, with a return of woodland by about 1000 yr B.P.

Analyses of pollen and land snails from rocksheter sites in the Osage Hills of northeastern Oklahoma indicate that the period 2000-1000 yr B.P. was moister than today. During that time, colonies of the prairie vole Microtus ochrogaster were present in the Texas Panhandle. About 1000 yr B.P. the climate changed to dry conditions that have persisted to the present. Disjunct colonies of small mammals in Texas became extinct at the beginning of the dry episode, thereby establishing the composition of the modern fauna. The climatic model for the origin of the Panhandle Aspect (A.D. 1200–1500) is questioned on the grounds that the Southern Plains experienced a long period of dry climate commencing A.D. 950.