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Characterization and in vitro evaluation of gelatin–chitosan scaffold reinforced with bioceramic nanoparticles for bone tissue engineering

Published online by Cambridge University Press:  27 May 2019

Kanchan Maji  and
Sudip Dasgupta
Kanchan Maji
Affiliation:
Department of Ceramic Engineering, National Institute of Technology, Rourkela, Odisha 769008, India
Sudip Dasgupta*
Affiliation:
Department of Ceramic Engineering, National Institute of Technology, Rourkela, Odisha 769008, India
*
a)Address all correspondence to this author. e-mail: dasguptas@nitrkl.ac.in
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Abstract

Gelatin–chitosan–based scaffolds using different bioactive nano-ceramic phase such as hydroxyapatite (HAp), beta tri calcium phosphate (β-TCP) and 58 s bioactive glass (58 s BG) were fabricated at a fixed 30 wt% of bioceramic phase content. From FTIR spectrum of the composite scaffold, a red shift in amide I and amide II bonds from 1595 to 1545 cm−1 and a new absorption peak due to electrostatic interaction between Ca2+ and COO− were observed. Average pore size in all the composite scaffolds was in the range between 100 and 300 μm, significantly smaller than the average pore size of pure gelatin–chitosan scaffold. Gelatin–chitosan-58 s BG (GCB30) scaffold exhibited the highest amount of protein absorption of 23 mg/cm2 among all the prepared scaffolds after 36 h of incubation in bovine serum albumin (BSA) solution. Mesenchymal stem cell’s (MSC’s) proliferation onto GCB30 scaffold was significantly higher as compared to other prepared scaffolds up to 7 days of cell culture. Expression of both early marker (RUNX2) and late marker (Osteocalcin) of differentiation was higher in MSCs cultured onto GCB30 scaffold as compared to other prepared scaffolds.

Keywords

biomaterialbonenanostructure

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Type
Article
Information
Journal of Materials Research , Volume 34 , Issue 16 , 28 August 2019 , pp. 2807 - 2818
Copyright
Copyright © Materials Research Society 2019 

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