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In-vitro fermentation of whole seaweed and a polysaccharide-rich extract derived from the edible red seaweed Palmaria palmate

Published online by Cambridge University Press:  20 June 2016

P. Cherry ,
S. Yadav ,
C. O'Callaghan ,
Z.A. Popper ,
R.P. Ross ,
E.M. McSorley ,
P.J. Allsopp  and
C. Stanton
P. Cherry
Affiliation:
Northern Ireland Centre for Food and Health, Ulster University, Coleraine, BT52 1SA, Northern Ireland
S. Yadav
Affiliation:
Teagasc Food Research Centre, Moorepark, Co. Cork, Republic of Ireland
C. O'Callaghan
Affiliation:
Ryan Institute, National University of Ireland Galway, Ireland
Z.A. Popper
Affiliation:
Ryan Institute, National University of Ireland Galway, Ireland
R.P. Ross
Affiliation:
Alimentary Pharmabiotic Centre, University College Cork, Republic of Ireland
E.M. McSorley
Affiliation:
Northern Ireland Centre for Food and Health, Ulster University, Coleraine, BT52 1SA, Northern Ireland
P.J. Allsopp
Affiliation:
Northern Ireland Centre for Food and Health, Ulster University, Coleraine, BT52 1SA, Northern Ireland
C. Stanton
Affiliation:
Teagasc Food Research Centre, Moorepark, Co. Cork, Republic of Ireland Alimentary Pharmabiotic Centre, University College Cork, Republic of Ireland
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Abstract

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Dietary fibre is considered an important component of a healthy diet, with evidence indicating that fibre may positively modulate gut microbiota composition and metabolism( Reference Simpson and Campbell1 ). There is increased attention upon the potential health benefits of seaweeds due to high fibre content( Reference MacArtain, Gill and Brooks2 ), where the commonly consumed red seaweed Palmaria palmata is of particular interest due to the presence of the fermentable fibre Xylan, previously shown to exert prebiotic effects( Reference O'Sullivan, Murphy and McLoughlin3 ). This study aims to provide initial indications of the impact of Palmaria palmata and its polysaccharide fibre component on the composition of the human microbiota.

This study used faecal batch culture models to assess the in-vitro fermentability of pre-washed, freeze dried, whole seaweed (WS) and polysaccharide-rich (PR) extracts of Palmaria palmata, alongside Synergy 1 (positive control) and cellulose (negative control). Treatments underwent an in-vitro simulated upper gastrointestinal digestion process followed by a 48 hour in-vitro batch culture faecal fermentation (1% w/v treatment). Enumeration of total bacteria, Lactobacilli and Bifidobacteria was determined using qPCR as well as culture based methods at all time points (t = 0, 5, 10, 24, 36 and 48 hours). Data was log-transformed prior to two-way ANOVA statistical analysis (n = 3).

qPCR analysis (Fig. 1–3): No significant differences were observed in total bacterial counts between substrates at any time point (Fig. 1; P > 0·1). Neither WS nor PR treatments showed a significant increase in Lactobacilli relative to cellulose (Fig. 2; P > 0·1). WS triggered a significant increase in Bifidobacteria relative to cellulose at 24 h and 36 h (Fig. 3; P < 0·05), whilst PR was shown to have a stimulatory effect at 24 h, 36 h and 48 h (P < 0·001). Further, PR samples were analogous to Synergy 1 at 48 h (P > 0·1). Similar trends were observed in the culture based analysis.

Fig. 1.

Total bacteria.

Fig. 2.

Lactobacilli.

Fig. 3.

Bifidobacteria.

These data suggest that freeze dried Palmaria palmata powder and polysaccharide-rich extracts of Palmaria palmata exhibit bifidogenic activity. Metagenomic sequencing of the microbial population and targeted metabolomics is required to verify these findings.

References

1. Simpson, HL & Campbell, BJ (2015) Aliment Pharmacol Ther 42, 158–179.Google Scholar
2. MacArtain, P, Gill, CIR, Brooks, M et al. (2007) Nutrition Reviews 65, 535–543.Google Scholar
3. O'Sullivan, L, Murphy, B, McLoughlin, P et al. (2010) Marine Drugs 8, 2038–2064.Google Scholar
Figure 0

Fig. 1. Total bacteria.

Figure 1

Fig. 2. Lactobacilli.

Figure 2

Fig. 3. Bifidobacteria.