Introduction
One of the principal causes of global biodiversity declines is habitat loss as a result of land conversion (Gibson et al., Reference Gibson, Lee, Koh, Brook, Gardner and Barlow2011; Joppa et al., Reference Joppa, O'Connor, Visconti, Smith, Geldmann and Hoffmann2016; Gosper et al., Reference Gosper, Fox, Burbidge, Craig, Douglas and Fitzsimons2019), mainly for agriculture (Benton et al., Reference Benton, Bryant, Cole and Crick2002; Koh et al., Reference Koh and Wilcove2008; Clay, Reference Clay2013). Intensive farming systems affect the dynamics of bird assemblages by causing the extinction of habitat specialists (Bretagnolle et al., Reference Bretagnolle, Siriwardena, Miguet, Henckel, Kleijn, Lemaire, Carvalho, Kronberg and Recous2019). Farmland birds and Galliformes in particular are declining across Europe because of agricultural intensification (Stoate et al., Reference Stoate, Boatman, Borralho, Carvalho, De Snoo and Eden2001; Wretenberg et al., Reference Wretenberg, Lindström, Svensson, Thierfelder and Pärt2006; Báldi & Batáry, Reference Báldi and Batáry2011).
By contrast, low intensity and subsistence agriculture have fewer negative effects on biodiversity, because of traditional, low impact farming practices and the preservation of more heterogeneous landscapes (Fox, Reference Fox2004; Laiolo et al., Reference Laiolo, Dondero, Ciliento and Rolando2004; Verhulst et al., Reference Verhulst, Báldi and Kleijn2004; Giupponi et al., Reference Giupponi, Ramanzin, Sturaro and Fuser2006; Assandri et al., Reference Assandri, Bogliani, Pedrini and Brambilla2018). Furthermore, forest edges resulting from habitat fragmentation following agricultural conversion can provide vital habitats for some forest birds (Imbeau et al., Reference Imbeau, Drapeau and Mönkkönen2003).
In Myanmar agriculture is still largely low intensity, with low inputs and minimal use of machinery, providing local subsistence crops in most parts of the country (FAO/WFP, 2016). With political change and accelerating human development, however, agricultural practice will probably have an increased impact on biodiversity in the near future. This is particularly important as c. 70% of the country's key biodiversity areas are outside legally protected areas (Tordoff et al., Reference Tordoff, Eames, Eberhardt, Baltzer, Davidson and Leimgruber2005). Traditional forest management by local communities is more effective than exclusive management by government (Nelson & Chomitz, Reference Nelson and Chomitz2009; Li et al., Reference Li, Wang, Yin, Zhaxi, Jiagong, Schaller and Xiao2014), helping communities integrate cultural values with benefits for both biodiversity and human well-being (Delisle et al., Reference Delisle, Kim, Stoeckl, Lui and Marsh2018; Infield et al., Reference Infield, Entwistle, Anthem, Mugisha and Phillips2018; Schneider, Reference Schneider2018).
The green peafowl Pavo muticus is categorized as Endangered because of hunting and habitat loss resulting from large-scale agricultural conversion (BirdLife International, 2014). Formerly ranging across much of South-east Asia, from north-east India to Java, Indonesia (Delacour, Reference Delacour1977; McGowan et al., Reference McGowan, Duckworth, Xianji, van Balen, Xiaojun, Khan and Kaul1998), its range and abundance have decreased dramatically and it now only survives in small isolated populations (Sukumal et al., Reference Sukumal, Dowell and Savini2020). In Myanmar the green peafowl mostly occurs outside protected areas and there is little information on its status since 1945 (BirdLife International, 2001). A viable population was recently found inhabiting a subsistence agriculture landscape surrounding an isolated forest patch occupied by a Theravada Buddhist monastery in Pwe Hla village, southern Shan State, Myanmar. In this area the species is not hunted by the local community in accordance with Buddhist precept. However, it is unclear how the population could be affected by possible future changes in agricultural practices.
Using the green peafowl as a model for species occurring in forest–agriculture edge habitats, we investigated how the animals respond to different crop patterns in an agricultural landscape surrounding a forest fragment managed by Buddhist monks. The objectives of this study were to (1) determine the density and abundance of the green peafowl in an agricultural area, (2) investigate the use of cropland by green peafowl living in forest fragments in an agricultural landscape and (3) present recommendations for sustainable management for this species in this area.
Study area
The study was conducted at Nan Kone Monastery, southern Shan State, Myanmar (Fig. 1). The area is at altitudes of 1,300–1,500 m, with a mean maximum monthly temperature of 33 °C in April and a mean monthly minimum of 15 °C in December, and annual rainfall of 1,921 mm (1–255 mm per month). The area has a cold dry season (December–February), a hot dry season (March–April) and a wet season (May–November; Htwe et al., Reference Htwe, Brinkmann and Buerkert2015). There are scattered native Ficus sp. trees that were planted over 100 years ago along the roads, and large water bodies are absent in the study area.
The study area at Nan Kone Monastery, near Nan Kone village, Pindaya township, southern Shan State, Myanmar, with habitat types and records of the green peafowl Pavo muticus. The 300 m buffer around the forest was used in the compositional analysis.
There are two distinct sections in the 2.872 km2 study area. In the eastern part, the remaining forest is little disturbed, big trees are absent, less land is cultivated and the landscape is dominated by open scrub. The western part is mostly used for growing crops, interspersed with large Ficus trees, and human disturbance (by farmers and villagers) is higher in this area (Fig. 1).
Methods
Habitat utilization and density estimation
We established three line transects with a total length of 3,414 m (transect 1 = 722 m, transect 2 = 1,176 m, transect 3 = 1,516 m): two (transects 1 and 2) in the eastern part of the forest patch and one (transect 3) in the western part (Fig. 1). We walked transects in both directions twice daily (07.00–09.00 and 16.00–18.00), on 4 consecutive days every month, for 12 months (February 2016–January 2017), at a speed of 1 km/h. Observers rotated between transects to avoid bias.
When a green peafowl was seen or heard, we recorded the number of individuals, whether they were adults or chicks, sex, the habitat being used and behaviour (feeding, displaying, roosting). We recorded the birds’ geographical location on paper maps and later entered the data into a GIS. We also recorded the presence of any natural predators and threats to the peafowl, and pesticide and herbicide use when we observed farmers spraying their fields.
We defined 11 micro-habitats (Table 1) in four main habitat categories: (1) forest, mainly in the area of the monastery, dominated by large pine and evergreen trees with an understory of thick, thorny scrub, (2) scrub, mainly in the eastern part of the study area, with rocky ground, sparse agave plants and thick thorny scrub, (3) cropland, consisting of rice paddy, radish, cabbage, potato, maize, bean, wheat and niger seed fields (Table 1), and (4) fallow, uncultivated fields with no crops grown during the study period and including marginal areas between croplands, ditches, bullock cart tracks and roads.
Habitat type, area cover and crop season in the Han Kone Monastery area, Pwe Hla, Pindaya township, Shan State, Myanmar (Fig. 1).
We digitized habitat boundaries from Google Earth (Google, Mountain View, USA) and conducted monthly checks by walking the transects before the surveys to confirm crop types and note any seasonal crop pattern changes (some crops such as potato and niger seed were alternated in the same patches during the study period). We calculated the coverage of each micro-habitat using ArcGIS 10.3 (Esri, Redlands, USA). Additionally, we asked farmers informal questions concerning their use of pesticides in the management of different crop types.
Data analysis
We estimated green peafowl density separately for the eastern and western parts of the study area, using a Hazard/Cosine model in DISTANCE 6.2 (Buckland et al., Reference Buckland, Anderson, Burnham, Laake, Borchers and Thomas2001). We analysed macro-habitat use by the green peafowl using compositional analysis, via Smith Ecology Compos Analysis 6.3a (Smith Ecology Ltd, Abergavenny, UK), which indicates any statistically significant preference or avoidance of habitat types compared to their availability, and ranks habitats in order of preference (Aebischer et al., Reference Aebischer, Robertson and Kenward1993; Bo et al., Reference Bo, Dowell, Garson and Fen-Qi2009). We substituted all proportional values (habitat use divided by habitat availability) of zero with 0.01 (Smith et al., Reference Smith2015).
To assess the relative importance of different habitats at the forest edge for peafowl, we created a 300 m wide buffer from the forest edge (this included 86% of all peafowl detections), combined all crop types in a single category and compared this against forest, scrub and fallow. We used a minimum of 1,000 iterations in all tests (Smith et al., Reference Smith2015).
Results
We detected the green peafowl a total of 359 times during the 12 months of the survey. Over this period, a total of five individual males were recorded in the area. Courtship displays were recorded during January–March. A nest with three eggs was found in May. We recorded chicks with females (five detections) during May–August. Dividing the study duration into periods of 4 months, the highest proportion of detections (calls and direct sightings) was during January–April (194; 54%), coinciding with the main display and mating period, and the lowest during the nesting period, May–August (37; 10%), when females were predominantly incubating eggs and males were not displaying. The remaining detections were in the non-breeding period, September–December (125; 36%).
Green peafowl density was 2.63 animals/km2 in the western and 1.13 animals/km2 in the eastern part (Table 2). The total population was estimated to be 22 individuals across the entire study area, with six individuals in the eastern and 16 in the western part (Table 2), although it is possible that individuals moved between the two parts of the study area.
Estimate of density and abundance of the green peafowl Pavo muticus in the eastern and western parts of the study area (Fig. 1), using distance sampling. The Table also shows the 95% confidence intervals of the estimates (CI) and the coefficient of variation of the abundance estimates (%CV).
Habitat selection in relation to availability was significantly non-random (Wilk's λ = 0.0115, χ 2 = 53.57, df = 10, P < 0.0001). A simplified ranking matrix (Table 3) indicates that forest was the most utilized habitat (P < 0.0001) and was significantly preferred over all crop types, except rice paddy. The second most utilized habitat was rice paddy and this was significantly preferred in relation to its availability compared to cabbage, radish, niger seed and wheat (Table 3).
Simplified ranking matrix from compositional analysis for all available habitat types across the entire study area, showing whether the habitat type in the row is selected (+), significantly selected (+++), avoided (−) or significantly avoided (---) relative to the habitat type in the column (t test P < 0.0001).
Of the 359 detections, 190 (53%) were in forest, and in all crop types combined there were only 36 detections (c. 10%). A simplified matrix presenting further details on habitat use, with all crop types combined into a single cropland category and compared against forest, scrub and fallow, is shown in Table 4. Habitats in order of preference were forest > cropland > scrub > fallow, but only forest was significantly preferred in relation to its availability. Habitat utilization was not significantly different between cropland, scrub and fallow.
Simplified ranking matrix for detections in all habitat types (including forest) and within the 300 m buffer zone from the forest edge (excluding forest), showing whether the habitat type in the row is selected (+), significantly selected (+++), avoided (−) or significantly avoided (---) relative to the habitat type in the column.
The forest patch formed the centre of the distribution of bird detections (Fig. 1) and the number of detections decreased with increasing distance from the forest (Fig. 2). To further examine the use of croplands at the forest edge, we defined a buffer zone around the forest. The majority (86.3%) of peafowl detections outside the forest were within 300 m of the forest edge (Fig. 1).
Distance from forest and number of green peafowl detections in cropland.
A comparison of habitat use and availability within the 300 m buffer showed that habitat selection was significantly non-random (Wilk's λ = 0.1369, χ 2 = 23.86, df = 2, P < 0.0001). A simplified ranking matrix (Table 4) indicates that the order of habitat preference was cropland > scrub > fallow (P < 0.0001), with cropland significantly preferred over the other two habitats. There was no significant difference between the utilization of scrub and fallow.
Discussion
Our density estimates from an agricultural landscape in Myanmar (2.63 and 1.13 birds/km2) are within the range of densities estimated in protected areas in other parts of the green peafowl's range: Yok Don National Park (0.25 calling birds/km2) and Cat Tien National Park (3.03 calling birds/km2) in Viet Nam (Sukumal et al., Reference Sukumal, McGowan and Savini2015); Siem Pang Wildlife Sanctuary (1.70 calling birds/km2; Loveridge et al., Reference Loveridge, Kidney, Ty, Eang, Eames and Borchers2017), and Seima Protection Forest (0.30 calling birds/km2; Nuttall et al., Reference Nuttall, Nut, Ung and O'Kelly2017) in Cambodia; and Huai Kha Khaeng Wildlife Sanctuary (1.13–11.34 calling birds/km2) in Thailand (Sukumal et al., Reference Sukumal, Dowell and Savini2017). However, our survey included sightings (of males and females) as well as auditory detection of calling birds, thus comparison with density estimates based on calling birds alone (which are primarily males) should be treated with caution.
Forest appears to be a key habitat for the green peafowl in our study area, although elsewhere the species is mostly found in open habitat with sparse ground vegetation cover, such as certain types of crops, shrub and fallow land. Studies in natural forest have shown the birds to occupy open areas within forest, such as alongside river beds, at certain times of the year (Sukumal et al., Reference Sukumal, Dowell and Savini2017). The main threat to the species in our study area is predation by feral dogs; we observed three breeding males being killed by dogs during February–March 2018. We also saw birds flying up into the branches of mature trees when attacked by dogs, suggesting forest provides some protection from predators. This could explain why peafowl primarily utilize areas within 300 m of the forest edge, as remaining close to trees could allow them to escape and reduce the risk of predation (Lawson & Johnson, Reference Lawson, Johnson, Chapman and Feldhamer1982).
Our findings also suggest that peafowl moving out of the forest prefer cropland over scrub and fallow land. Our data are based on both observations and call records from the different habitats. Detectability within cropland and fallow areas was similar and the scrub consisted of small patches of scattered bushes, where peafowl were highly visible. Croplands are potential feeding grounds for adult birds and can provide the invertebrates that are the main component of the diet of Galliformes chicks in the first 6–8 weeks (Potts, Reference Potts1986; Rands, Reference Rands, Hudson and Rands1988). The scrub habitat type in the study area could provide fewer feeding opportunities (Sukumal et al., Reference Sukumal, McGowan and Savini2015). Fallow land potentially harbours insect food, but the vegetation may be too dense for young chicks, which are vulnerable to chilling when brushing against wet vegetation (Reynolds et al., Reference Reynolds, Angelstam, Redpath, Hudson and Rands1988). The dense structure of this habitat may also compromise the birds’ ability to detect approaching predators (Reynolds et al., Reference Reynolds, Angelstam, Redpath, Hudson and Rands1988).
Given the apparent importance of cropland for feeding, the use of pesticides probably presents a threat to the species, through direct toxicity or via their effect on the supply of invertebrate food, thus potentially affecting chick survival rate (Stanton et al., Reference Stanton, Morrissey and Clark2018). In Galliformes such effects have been reported for the grey partridge Perdix perdix (Potts, Reference Potts1986; Warren et al., Reference Warren, Hornby and Baines2017), red-legged partridge Alectoris rufa and pheasant Phasianus colchicus (Rands, Reference Rands1986). The use of pesticides also directly affected adult mortality in the blue peafowl Pavo cristatus (Panigrahy et al., Reference Panigrahy, Grumbles and Hall1979) and northern bobwhite Colinus virginianus (Ertl et al., Reference Ertl, Mora, Brightsmith and Navarro-Alberto2018). In addition, agricultural intensification affects other ground-dwelling birds such as the South American Tinamiformes (Thompson, Reference Thompson2004). According to information obtained from the local farming community, pesticides (both herbicides and insecticides) are used on cabbage, beans, radish and potato crops in the area, but not on rice paddy, wheat, niger seed and maize (Table 1). Our findings suggest the birds show a preference for rice paddy, one of the crops that is not currently treated with pesticides, over other crop types (Table 3).
Agricultural landscapes are often considered to be of little conservation value (Sreekar et al., Reference Sreekar, Zhang, Xu and Harrison2015), but our study highlights the importance of small patches of forest outside protected areas for the survival of threatened species. In addition to the green peafowl we recorded mammals such as the barking deer Muntiacus muntjak and yellow throated martin Martes flavigula in the small forest patch, and 81 bird species. Habitat fragments outside legally protected areas are becoming increasingly important for biodiversity conservation in regions of rapid human population growth such as South-east Asia (Sodhi et al., Reference Sodhi, Pin, Clements, Wanger, Hill, Hamer and Ming2010). The conservation value of our study site may be typical for the wider Shan State landscape, where there are several remnant isolated forest patches surrounded by traditional agricultural areas. However, Myanmar's rapid economic development could change this matrix in the near future if traditional small-scale agriculture is converted to industrialized monocultures, as observed in neighbouring countries.
An important factor supporting the conservation of the green peafowl at the Pwe Hla study site is the strong influence of the resident Theravada Buddhist clergy on the local community (Gogoi, Reference Gogoi2018; Schneider, Reference Schneider2018). Abstinence from hunting the green peafowl can be attributed directly to the belief that the species represents one of the 108 distinguishing marks on the soles of the Buddha's feet. The protection provided by religious beliefs needs to be considered when planning future conservation efforts for this species.
The subpopulation of green peafowl at Pwe Hla is a small but significant one for this Endangered species, and is potentially part of a larger population occurring outside protected areas in the agricultural landscape of Shan State and beyond. To ensure the sustainable management of this population we recommend restricted use of agricultural pesticides, particularly around patches of remnant forests which provide roosting and nesting sites for the species. We also recommend control of feral dogs and continued support for the Buddhist traditions that provide protection for the birds from hunting and trapping.
Acknowledgments
We thank Pwe Hla Environmental Conservation and Development and Friends of Wildlife for their support, the honourable monks at Nan Kone Monasteries for granting permission to use the surrounding area for the survey and for their support, Scott Wilson for his support in GIS work, and Matthew Grainger for valuable comments and suggestions. NMS was sponsored by a Rufford Small Grant (19271-1), and the Conservation Ecology Program, King Mongkut's University of Technology Thonburi, Thailand, that provided PhD scholarship for NMS (18/2558). NS was supported by the Royal Golden Jubilee PhD Program, Thailand (PHD/0105/2553). We thank Fauna & Flora International for permission to NMS to conduct this research.
Author contributions
Study design: NMS, NS, TS; data collection: NMS, KMO; data analysis and writing: NMS, NS, SD, SB, TS.
Conflicts of interest
None.
Ethical standards
This research abided by the Oryx guidelines on ethical standards. It was observational and designed to ensure wildlife, study area and local communities were not harmed or disturbed.
Open access
