Spasticity is a sensorimotor control disorder that results from an upper motor neuron lesion that presents as intermittent or sustained involuntary muscle activation that can have a significant impact on a patient’s active or passive function. ^{Reference Pandyan, Gregoric and Barnes1} The flexor digitorum profundus (FDP) is a commonly targeted muscle for botulinum toxin A (BoNT-A) in patients with spasticity due to finger in palm deformity involving the distal interphalangeal joints of digits 2–5, and wrist flexion in patients with focal spasticity. Focal dystonias of the forearm can also be treated successfully with botulinum toxin. The objective of this paper is to demonstrate how the anatomical findings of Campisi et al. ^{Reference Campisi, Tran, Amara, Switzer-McIntyre and Agur2} have been translated to a clinical setting to enhance injection protocols of BoNT-A for the management of FDP post-stroke spasticity.

Previous anatomical studies that describe the morphology of the digital bellies of FDP are scarce and primarily descriptive. In a volumetric 3D anatomical study, ^{Reference Campisi, Tran, Amara, Switzer-McIntyre and Agur2} the intramuscular innervation of each of the digital bellies of FDP was mapped using cartesian coordinate data obtained from dissection and digitization of the digital bellies and intramuscular innervation as in situ. In contrast to previous descriptive 2D studies, this volumetric study found that the 3rd, 4th, and 5th digital bellies were superficial to the 2nd in all specimens (Fig. 1a,b). The 2nd digital belly was found to be innervated by the anterior interosseus nerve, which continued intramuscularly to supply the 3rd digital belly distally in all specimens. The proximal part of the 3rd digital belly was innervated by a branch of the ulnar nerve (UN). The entire 4th digital belly received innervation from a branch of the UN that continued intramuscularly to supply the more inferiorly located 5th digital belly in approximately half of the specimens. In the remaining specimens, the 5th digital belly was supplied by a branch of the UN only (Fig. 1a,b).

Figure 1:

3D mapping of the intramuscular innervation of the digital bellies of flexor digitorum profundus for botulinum toxin injection. a. 3D model of the digital bellies and their intramuscular innervation. Note the 3^rd, 4^th and 5^th digital bellies lie superficial to the 2^nd belly, proximally. b. 3D model of the intramuscular innervation of the 2^nd digital belly. c. Targeting of each digital belly for botulinum toxin injection. Transverse section at the level of the junction of the proximal and middle third of the length of the forearm. First, the needle is directed in a slightly deeper angle to inject the 2^nd digital belly (*) and then drawn back and redirected more superficially to inject the 3^rd, 4^th, and 5^th digital bellies (**). AIN = anterior interosseus nerve; APL = abductor pollicis longus; BR = brachioradialis, BT = biceps tendon; ECR = extensor carpi radialis; ECU = extensor carpi ulnaris; ED = extensor digitorum; EPL = extensor pollicis longus; FCR = flexor carpi radialis; FCU = flexor carpi ulnaris; FDS = flexor digitorum superficialis; FPL = flexor pollicis longus; ME = medial epicondyle; R = radius; U = ulna; UN = ulnar nerve.

Previous cadaveric studies have proposed BoNT-A injection sites based on nerve entry points into FDP, or described the sites based on injection into the muscle belly as a whole. ^{Reference Hwang, Jin, Hwang, Lee and Han3–Reference Yang, Zhang, Xie, Yang, Xu and Xie6} Campisi et al., ^{Reference Campisi, Tran, Amara, Switzer-McIntyre and Agur2} based on the findings of this volumetric study, proposed one needle entry site for BoNT-A at the junction of the proximal and middle third of the length of the forearm. Sihler’s staining studies have also reported “nerve terminal dense zones” in the proximal third of the muscle belly. ^{Reference Won, Hur and Rha5,Reference Yang, Zhang, Xie, Yang, Xu and Xie6} Fractional distribution of injectate into each of the digital bellies at this site could also target the nerve endings/ motor end plates.

Importantly, instrumental guidance has been recommended as standard practice for the BoNT-A injection technique. In a randomized control trial by Picelli et al. ^{Reference Picelli, Lobba and Midiri7} stroke patients with wrist and finger spasticity were randomized to receive BoNT-A injections using manual needle placement (palpation technique), electrical stimulation guidance, or ultrasonography guidance. The groups that received injection using electrical stimulation or ultrasonography demonstrated significant improvements in Modified Ashworth Scale scores, Tardieu angle, and passive range of motion compared to patients in the manual needle placement group. ^{Reference Picelli, Lobba and Midiri7} Furthermore, a systematic review concluded that ultrasound may lead to better visualization of targeted muscles, and thus improve the accuracy of injection and potentially improve clinical outcomes. ^{Reference Chan, Finlayson and Mills8} Although electromyography and electrical stimulation can improve injection accuracy, they rely on anatomical surface landmarking, especially for initial needle insertion. Abnormal posturing of spastic limbs can lead to distortion of the associations between muscle targets and the surface landmarks, rendering the commonly held injection approaches to have lower accuracy. ^{Reference Chan, Finlayson and Mills8} One of the main advantages of ultrasound is to verify the approach to the target prior to needle insertion. Thus, specific structures can be avoided so that there is less change in needle position during the injection, as compared to electromyography and needle stimulation, which often require repositioning of the needle. Ultrasonography also provides continuous visualization of the target muscles to tract the path of the needle and to verify the location of injection.

Our first clinical pearl highlights the importance of the anatomical relationship of the 3^rd, 4^th and 5^th digital bellies of FDP being superficial to the 2^nd digital belly. This has implications for injection targeting and appropriate fractional distribution of BoNT-A into FDP. The authors recommend a single needle entry point at the junction of the proximal and middle third of the length of the forearm, one finger’s breadth anterior to the ulna (Fig. 1). First, the needle is directed in a slightly deeper angle to inject the 2^nd digital belly, and then drawn back and redirected more superficially to inject the 3^rd and then 4^th and 5^th digital bellies as the needle is drawn back towards exiting (Fig. 1c). The ulnar artery and nerve are located just superficial to the 3^rd digital belly and should be avoided along with flexor carpi ulnaris (Fig. 1c). The proposed injection approach could be further guided by electromyography and/or electrical stimulation to verify individual muscle belly needle entry. However, the authors suggest direct visualization under ultrasound guidance could provide a more targeted injection, particularly for patients in which surface landmarks are difficult to verify.

Our second clinical pearl focuses on the authors’ recommendation of fractional distribution of injection into the FDP muscle. Although a single large injection volume into the FDP muscle could in theory be used, it would be unlikely to distribute BoNT-A evenly or adequately throughout the entire muscle. Rather, it may be more effective to inject into each digital belly by placing the needle tip into each belly to deliver the appropriately desired dose/volume of neurotoxin. Thus, the practitioner could more effectively distribute the injectate throughout the muscle or into specific individual bellies to optimize outcomes for difficult to treat flexed spastic hand deformity, or in cases of functional spastic or dystonic hand in which sparing of certain digits for function would be of paramount importance.

In conclusion, the authors suggest the recommended needle entry site and injection pattern (Fig. 1), ideally paired with ultrasound guidance, could serve to optimize BoNT-A delivery and avoid misplacement of the injection(s). We plan to further study the injection accuracy of this recommended injection approach and report on the findings in future publications.