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MALDI-TOF MS protein profiling for the rapid identification of Chagas disease triatomine vectors and application to the triatomine fauna of French Guiana

Published online by Cambridge University Press:  03 August 2017

MAUREEN LAROCHE ,
JEAN-MICHEL BÉRENGER ,
GLADYS GAZELLE ,
DENIS BLANCHET ,
DIDIER RAOULT  and
PHILIPPE PAROLA
MAUREEN LAROCHE
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
JEAN-MICHEL BÉRENGER
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
GLADYS GAZELLE
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
DENIS BLANCHET
Affiliation:
Laboratoire Hospitalier Universitaire de Parasitologie et Mycologie, Centre Hospitalier A. Rosemon, Cayenne, France
DIDIER RAOULT
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
PHILIPPE PAROLA*
Affiliation:
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Aix-Marseille University, UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France
*
*Corresponding author: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), Faculté de Médecine, 19-21 bd Jean Moulin, 13385 Marseille cedex 5, France. E-mail: philippe.parola@univ-amu.fr
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Summary

Triatomines are haematophagous insects involved in the transmission of Trypanosoma cruzi, the aetiological agent of Chagas disease. The vector competence of these arthropods can be highly variable, depending on the species. A precise identification is therefore crucial for the epidemiological surveillance of T. cruzi and the determination of at-risk human populations. To circumvent the difficulties of morphological identification and the lack of comprehensiveness of the GenBank database, we hereby propose an alternative method for triatomine identification. The femurs of the median legs of triatomines from eight different species from French Guiana were subjected to matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. Method evaluation was performed on fresh specimens and was applied to dry specimens collected between 1991 and 2003. Femur-derived protein extracts provided reproducible spectra within the same species along with significant interspecies heterogeneity. Validation of the study by blind test analysis provided 100% correct identification of the specimens in terms of the species, sex and developmental stage. MALDI-TOF mass spectrometry appears to be a powerful tool for triatomine identification, which is a major step forward in the fight against Chagas disease.

Keywords

TriatominaeMALDI-TOFFrench Guianaarthropod identification

Information

Type
Special Issue Article
Information
Parasitology , Volume 145 , Special Issue 5: Advances and challenges in barcoding of microbes, parasites, and their vectors and reservoirs , April 2018 , pp. 665 - 675
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Copyright © Cambridge University Press 2017 

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