A Mediterranean (MED) diet is the traditional dietary pattern observed in countries bordering the Mediterranean Sea. It is characterised by a high consumption of fruits, vegetables, unrefined cereals, legumes, nuts and seeds, olive oil, a moderate wine consumption and lower intake of animal products. The MED diet was proposed as a healthy dietary pattern based on the evidence of its protective role in some chronic diseases(Reference Trichopoulou, Costacou and Bamia1–Reference Martinez-Gonzalez, Bes-Rastrollo and Serra-Majem5). As these plant-based products are high in flavonoids(Reference Neveu, Perez-Jimenez and Vos6, 7), a possible link between flavonoids and reported health benefits may be considered.
Flavonoids constitute a wide group of secondary plant metabolites that are ubiquitously distributed in the plant kingdom. Flavonoids are polyphenolic compounds possessing fifteen carbons, with two aromatic rings (rings A and B) joined by a linear three-carbon chain usually forming a closed pyran ring (ring C). According to the modifications of the central C-ring, they are often divided into six classes: anthocyanidins, flavonols, flavanones, flavones, isoflavones and flavanols or flavan-3-ols, including monomers, proanthocyanidins (PA) (oligomers and polymers of flavan-3-ol monomers) and flavanol-derived compounds (theaflavins and thearubigins)(Reference Manach, Scalbert and Morand8).
Despite their low-to-moderate bioavailability, high inter- and intra-individual variation in absorption and rapid conjugation(Reference Manach, Williamson and Morand9), flavonoids have been extensively studied in the last two decades due to their potential health effects, such as antioxidant, anti-inflammatory, anticarcinogenic, anti-obesity, anti-allergic and anti-diabetic properties(Reference Cazarolli, Zanatta and Alberton10, Reference Yao, Jiang and Shi11). Associations between the consumption of total or classes of flavonoids and chronic disease prevention have also been suggested in several case–control and a few cohort studies(Reference Arts and Hollman12, Reference Knekt, Kumpulainen and Jarvinen13). Epidemiological evidence of the role of flavonoid intake against the risk of some chronic diseases is promising, but not conclusive. Complete descriptive studies of dietary intake are the first step in the assessment of the potential relationship between flavonoids and health.
To date, total flavonoid intake has been estimated in a few descriptive studies (Table 1)(Reference Chun, Chung and Song14–Reference Hertog, Hollman and Katan24). Moreover, these results varied widely among the studies because of differences in the flavonoid classes included, food composition tables used and dietary assessment methodologies utilised. It is difficult, therefore, to compare the existing data, and the present total dietary flavonoid estimations do not yet allow us to conclude which countries, i.e., MED or non-MED countries, consume more flavonoids. Hence, the present study aimed to estimate total flavonoid intake among European adults from MED and non-MED countries participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Furthermore, the present study also aimed to assess the main flavonoid food sources and lifestyle characteristics that could partly explain the flavonoid intake variability among these countries.
Estimated flavonoid intake in adults in several countries
FCDB, food composition database; PA, proanthocyanidins; SUVIMAX, SUpplémentation en VItamines et Minéraux Anti-oXydants Study; USDA, United States Department of Agriculture; EPIC, European Prospective Investigation into Cancer and Nutrition; NHANES, National Health and Nutrition Examination Survey; 24-HDR, 24 h dietary recall; FINDIET, National Finnish Diet Study; 48-HDR, 48 h dietary recall.
* Database with dihydroflavonols which were included in total flavonoid intake presented in this table.
† USDA database on flavonoids, version 1, March 2003.
‡ Thearubigins were included in total flavonoid intake presented in this table.
§ USDA database on flavonoids, version 2.1, January 2007.
∥ Theaflavins and thearubigins were not included in total flavonoid intake presented in this table.
¶ Calculated taking into account flavonoid intake without PA, because PA intake was not available at the time the study was done.
** Recalculated using USDA databases by Johannot & Somerset(Reference Johannot and Somerset21).
Materials and methods
Study population
The EPIC study is an ongoing prospective cohort study conducted in twenty-three centres throughout ten European countries to investigate the role of nutrition, lifestyle, biomarkers and genetic factors in the aetiology of cancer and other chronic diseases(Reference Riboli and Kaaks25, Reference Riboli, Hunt and Slimani26). A total of 521 448 subjects (29·4 % men), aged 21–83 years, were enrolled between 1992 and 2000. Most of the participants were recruited from the general population within defined geographical areas, with some exceptions: women who were members of a health insurance programme for state school employees (France), women attending breast cancer screening (Utrecht, the Netherlands and Florence, Italy), blood donors (some centres in Italy and Spain) and vegetarians (the ‘health conscious’ cohort in Oxford, UK). For the purpose of dietary analyses, the twenty-three administrative EPIC centres were redefined into twenty-seven geographical areas(Reference Slimani, Kaaks and Ferrari27). A total of nineteen of the twenty-seven redefined EPIC centres had both male and female participants, and eight recruited only women (France; Norway; Utrecht, The Netherlands; and Naples, Italy).
Dietary data used in the present paper were obtained from the EPIC calibration study, in which a 24 h dietary recall (24-HDR) was administered to a stratified random sample of approximately 8 % (36 994 subjects) of the entire EPIC cohort(Reference Slimani, Kaaks and Ferrari27). A total of 35 628 subjects with 24-HDR data from twenty-six centres were included in the present analysis, after exclusion of sixteen subjects due to missing baseline FFQ data, 941 subjects aged less than 35 years or over 74 years (because these age groups had low representation) and all participants from the health conscious group (409 subjects), because vegetarians and vegans consume a very different diet from the rest of the cohort(Reference Slimani, Fahey and Welch28). The present study was conducted according to the guidelines laid down in the Declaration of Helsinki, and all procedures involving human subjects were approved by ethical review boards from the International Agency for Research on Cancer (IARC) and all local participating centres approved the study. Written informed consent was obtained from all subjects.
Dietary and lifestyle information
Dietary information was obtained through a single standardised 24-HDR interview using computerised software (EPIC-Soft®; International Agency for Research on Cancer)(Reference Slimani, Deharveng and Charrondiere29, Reference Slimani, Ferrari and Ocke30), which was administered face-to-face in all centres, except in Norway, where it was done by telephonic interview(Reference Brustad, Skeie and Braaten31). In the EPIC study, about 2000 aggregated food items were reported per country in the 24-HDR, of which approximately forty food items were not present in our food composition database (FCDB). Most of these food items, except coffee substitutes and cola drinks, do not contain flavonoids in their composition (such as water, sugar, margarine, caramel, white chocolate, tonic soft drink, chewing gum, artificial sweetener, salt and gelatine) or they are infrequently consumed in the EPIC population (such as bamboo sprouts, carambola, pumpkin flower, orgeat, rose hips, physalis and vanilla). Data on other lifestyle factors, including education, anthropometry, physical activity (combining both occupation, household and leisure time activities) and smoking history, were collected at baseline through standardised questionnaires and have been described elsewhere(Reference Riboli, Hunt and Slimani26, Reference Slimani, Kaaks and Ferrari27, Reference Wareham, Jakes and Rennie32). Data on age, as well as on body weight and height, were mostly self-reported by the participants during the 24-HDR interview. The mean time interval between these baseline measures and the 24-HDR interview varied by country, from 1 d to 3 years later(Reference Slimani, Kaaks and Ferrari27).
Flavonoid Food Composition Database
Our FCDB gathered composition data on six flavonoid classes: anthocyanidins (cyanidin, delphinidin, malvidin, pelargonidin, peonidin and petunidin), flavanols (flavan-3-ol monomers (catechin, epigallocatechin, epicatechin, epicatechin-3-gallate, epigallocatechin-3-gallate, gallocatechin and catechin-3-gallate), PA (dimers, trimers, 4–6-mers, 7–10-mers and >10-mers) and theaflavins (theaflavin, theaflavin-3,3′-digallate, theaflavin-3′-gallate and theaflavin-3-gallate)), flavonols (isorhamnetin, kaempferol, myricetin and quercetin), flavones (apigenin and luteolin), flavanones (eriodictyol, hesperetin and naringenin) and isoflavones (daidzein, genistein, glycetin, biochanin A, formononetin and equol)(Reference Zamora-Ros, Knaze and Luján-Barroso33–Reference Zamora-Ros, Knaze and Luján-Barroso36). Thearubigins were not included in the present study because they were obtained by a non-specific spectrophotometric method(7).
Flavonoid data were mainly obtained from the United States Department of Agriculture FCDB on flavonoids (version 2.1 released in 2007, although more recently a new version has been developed in 2011)(7), isoflavones (updated in 2008)(37) and PA (released in 2004)(38), and the Phenol-Explorer FCDB (developed in 2009)(Reference Neveu, Perez-Jimenez and Vos6) and the UK Food Standards Agency FCDB on isoflavones (released in 2010)(Reference Ward, Kuhnle and Mulligan39). The United States Department of Agriculture and Phenol-Explorer FCDB contain flavonoid data from a systematic and comprehensive collection of worldwide analytical data. Furthermore, our flavonoid FCDB was expanded by using retention factors, calculating flavonoid content of recipes, estimating missing values based on similar foods (by botanical family and plant part), obtaining consumption data for food group items and employing botanical data for logical zeros. The retention factors applied to all flavonoid classes, except isoflavones, were 0·70, 0·35 and 0·25 after frying, cooking in a microwave oven and boiling, respectively(Reference Crozier, Lean and McDonald40). These retention factors were not applied to isoflavones, because their cooking losses are usually minimal(Reference Horn-Ross, Barnes and Lee41). The final FCDB on flavonoids contained 1877 food items and 10 % of these food items had missing values.
Statistical analyses
Dietary flavonoid values were calculated using generalised linear models and presented as means with standard errors stratified by sex and the redefined centres, which were ordered geographically from south to north. These models were adjusted for age (continuous variable) and weighted by season and weekday of the 24-HDR to control for different distributions of participants across seasons and weekdays of the recall. The contribution of each individual compound and class of flavonoids to the total intake was calculated as a percentage according to the two European regions (MED countries: all centres in Greece, Spain, Italy and the south of France; non-MED countries: all centres in the north-east and north-west of France, Germany, the Netherlands, UK general population, Denmark, Sweden and Norway). The contribution of each food group to overall flavonoid intake by European region was also computed as a percentage.
Differences in flavonoid intakes stratified by European region were also compared using general linear models according to the categories of sex, age (35–44, 45–54, 55–64 or 65–74 years), BMI ( < 25, 25 to < 30 or ≥ 30 kg/m2), educational level (none, primary completed, technical/professional, secondary school or university degree), smoking status (never smoker, present smoker or former smoker) and level of physical activity (inactive, moderately inactive, moderately active or active). All these models were adjusted for sex (categorical), age (continuous), centre (categorical), BMI (continuous) and energy intake (continuous) and weighted by season and weekday. P values < 0·05 (two-tailed) were considered significant. All analyses were conducted using the SPSS Statistics software (version 19.0; SPSS Inc.).
Results
For both sexes, consumers with the highest total flavonoid intake were from the UK general population (in men 548·8 mg/d and in women 501·7 mg/d). Meanwhile, consumers with the lowest total flavonoid intake were in Greece (in men 250·7 mg/d and in women 203·6 mg/d; Fig. 1). There was no statistically significant difference in flavonoid intake when adjusted for sex, age, centre, BMI and energy consumption and weighted by season and weekday of 24-HDR between the MED region (370·2 mg/d) and the non-MED region (373·7 mg/d; P= 0·349; Table 2). Men had a statistically higher intake of total flavonoids than women in MED countries, while the inverse was found in non-MED countries. Younger people had significantly lower flavonoid consumption than older people in MED and non-MED regions. An inverse trend between BMI and total flavonoid intake was observed in both regions. According to educational level, participants with technical/professional studies and university graduates had the highest consumption of flavonoids in MED and non-MED countries, respectively. Never and former smokers had the highest flavonoid intakes in both MED and non-MED countries. Physically active participants had the highest intakes, particularly in the MED region.
Adjusted daily flavonoid intake (mg/d), stratified by sex and centre ordered from south to north, adjusted for age and weighted by season and weekday of dietary recall. GRE, Greece; SPA, Spain; ITA, Italy, FRA, France, GER, Germany; NED, The Netherlands; DEN, Denmark; SWE, Sweden; NOR, Norway; ■, men; 
, women. Values are means, with standard errors represented by vertical bars.
Estimated mean total flavonoid intake (mg/d) in Mediterranean (MED) and non-MED countries of the European Prospective Investigation into Cancer and Nutrition cohort by socio-demographic and lifestyle factors* (Mean values with their standard errors)
* Adjusted for sex, age, centre, energy intake and BMI and weighted by season and weekday of recall. P value is for differences in means.
† Differences between each stratification variable in each region.
‡ Differences between MED-countries and non-MED countries.
Table 3 shows the flavonoid class contributors to the total flavonoid intake. Flavanol class was the main contributor in both regions, ranging from 72·5 to 75·0 %. More specifically, in MED countries, PA contributed 59·0 % and flavan-3-ol monomers only 13·1 %, whereas in non-MED region, PA and flavan-3-ol monomers contributed 48·2 and 24·9 % respectively. Flavonols, flavanones and anthocyanidins were intermediate contributors in all regions (7·3–10·1 %). Finally, the contributions of flavones and isoflavones were minor ( < 1·5 %).
Percentage contribution of classes of total flavonoid intake in the European Prospective Investigation into Cancer and Nutrition cohort*
MED, Mediterranean.
* Adjusted for age, sex and weighted by season and weekday of dietary recall.
The main food sources of dietary flavonoid intake according to European region are presented in Table 4. In non-MED countries, tea and fruits were the main food items contributing 25·7 and 32·8 % of total flavonoid intake, respectively. Other moderate contributors consisted of wines, juices, cereals, sweets and chocolate products. However, in MED countries, fruits (55·1 %, mainly apples and pears), wines (16·7 %), tea (6·8 %) and vegetables (4·5 %) were the most important food sources.
Percentage contributions of food groups and some main foods to the intake of flavonoids by European region*
* Values are percentages derived from models adjusted for age and sex and weighted by season and weekday of recall.
Discussion
The present study is the first large descriptive study presenting differences in the total dietary flavonoid intake, the related food sources and socio-demographic and lifestyle determinants between MED and non-MED countries. The results could be readily compared among centres and among regions, because a standardised 24-HDR (EPIC-Soft) and a common flavonoid FCDB were used for the entire EPIC cohort.
In the present study, there was no significant difference in total flavonoid intake between MED (370·2 mg/d) and non-MED countries (373·7 mg/d; P< 0·349), although a large variation in flavonoid intake and food sources was observed among centres. In MED countries, a wide range in total dietary flavonoids was found, ranging from 203·6 mg/d for Greek women to 527·6 mg/d for men from Turin (Italy). The highest flavonoid class contributor was by far PA (59·0 %), with its main food sources being fruits, wines and tea(Reference Knaze, Zamora-Ros and Luján-Barroso35), as was also reported in an Italian case–control study of gastric cancer(Reference Rossi, Rosato and Bosetti17) and in the Spanish EPIC cohort(Reference Zamora-Ros, Andres-Lacueva and Lamuela-Raventos19). Greek men and women were the lowest flavonoid consumers in the present study and these findings are consistent with a recent study(Reference Dilis and Trichopoulou18). Furthermore, a south to north gradient of increasing total flavonoids was observed within Spain and Italy, as previously described in the EPIC-Spain cohort(Reference Zamora-Ros, Andres-Lacueva and Lamuela-Raventos19). This gradient could be due to participants in northern areas of MED countries consuming more apples and pears and red wine than southern areas(Reference Agudo, Slimani and Ocke42, Reference Sieri, Agudo and Kesse43).
In non-MED countries, there was also a broad range of flavonoid intakes from 275·7 mg/d for women from Malmö (Sweden) to 548·8 mg/d for men of the UK general population. In this region, the main food sources were clearly tea (25·7 %) and fruits (32·8 %) and the major flavonoid class contributors were PA and flavan-3-ol monomers. Countries where tea is widely consumed, such as the UK, The Netherlands, Denmark or Australia will generally have high flavonoid intake, as a result of the sum of flavan-3-ol monomers and flavanol-derived compounds (theaflavins and thearubigins), the latter being the highest contributing class(Reference Johannot and Somerset21–Reference Hertog, Hollman and Katan24). However, in the present study, thearubigins were not included because they were obtained by an unsuitable method(7). Excluding thearubigins from the analysis, PA became the major flavonoid contributor in the non-MED region, especially in countries where tea consumption is less prevalent, for instance, in our Scandinavian centres (data not shown) or in a recent Finnish study.(Reference Ovaskainen, Torronen and Koponen16).
Differences between MED and non-MED countries were also explained by some socio-demographic, anthropometric and lifestyle factors. With respect to sex, men had a higher flavonoid intake than women in MED countries as men tend to drink more wine, particularly red wine(Reference Zamora-Ros, Andres-Lacueva and Lamuela-Raventos19). However, in non-MED countries, the opposite was found, as women tended to consume more tea than men(Reference Arts, Hollman and Feskens44). Flavonoid intake was also shown to increase with age in both these regions(Reference Zamora-Ros, Andres-Lacueva and Lamuela-Raventos19, Reference Arts, Hollman and Feskens44). Again, the observed differences were largely due to variations in wine and tea consumption. A US study(Reference Chun, Chung and Song14) showed that flavonoid intake increased up to the age group of 51–70 years, but in the age group >70 years, the consumption decreased significantly. In the present study, this reduction was also clearly detected in the oldest age group (65–74 years). Also, in the present study, participants living in the non-MED region and having a BMI of less than 25 kg/m2 had a significantly higher flavonoid consumption than those in any other BMI groups. In the MED countries, no statistical difference was observed between normal and overweight participants and flavonoid intake. However, in a previous analysis from the EPIC-Spain cohort, overweight people had the highest flavonoid intake; the difference was small but statistically significant(Reference Zamora-Ros, Andres-Lacueva and Lamuela-Raventos19). In the non-MED region, less-educated adults had the lowest flavonoid intake, while people with a university degree had the highest; thus, flavonoid intake seems to increase with higher educational level. In contrast, in an US study, flavonoid intake was higher among those with a higher poverty income ratio(Reference Chun, Chung and Song14). People with a higher education and/or income are usually more conscious of good nutrition and health, so they may take more care of themselves and consume more healthy foods such as fresh fruit and vegetables(Reference James, Nelson and Ralph45, Reference Schroder, Marrugat and Covas46). However, in MED countries, the highest flavonoid intake was observed in the subjects of technical/professional education, who also tended to have the highest wine intakes(Reference Marques-Vidal and Dias47). Never and former smokers had a higher flavonoid intake than present smokers in both MED and non-MED countries, as was observed previously in the EPIC-Spain cohort(Reference Zamora-Ros, Andres-Lacueva and Lamuela-Raventos19). However, smoking status was not associated with flavonoid intake in the USA(Reference Chun, Chung and Song14). In both regions, the trend of increasing flavonoid intake with more physical activity was probably observed because physically active people generally consume more fruits and vegetables, as shown in the EPIC-Spain cohort(Reference Agudo and Pera48).
Since the in vitro evidence on health effects of flavonoids was published(Reference Cazarolli, Zanatta and Alberton10, Reference Yao, Jiang and Shi11), some epidemiological studies have reported inverse associations between flavonoid intake and chronic diseases such as CVD(Reference Hooper, Kroon and Rimm49) and some cancers(Reference Rossi, Rosato and Bosetti17, Reference Arts, Jacobs and Gross50–Reference Rossi, Negri and Talamini53). Despite the comparable flavonoid intakes between MED and non-MED countries in the present study, there is a well-established south to north gradient of CVD mortality(Reference Levi, Lucchini and Negri54). This might be explained in part by the different consumption patterns of flavonoid classes and their food sources, as observed in the present study. In addition, other established dietary and lifestyle risk factors of CVD are more prevalent in non-MED countries(Reference Martinez-Gonzalez, Bes-Rastrollo and Serra-Majem5, Reference Jakes, Day and Khaw55, Reference Linseisen, Welch and Ocke56). Further basic and epidemiological studies are needed to clarify the potential role of flavonoids against CVD and other chronic diseases.
The present study has a number of relevant strengths. The first is the large number of participants in the EPIC calibration subcohort. The second is the complete list of flavonoids used. The third strength is the use of the most updated and relatively large FCDB on worldwide analytical flavonoid data, which was expanded with plant–plant estimations, thus obtaining a final database with 1877 food items with only 10 % of unknown values. The fourth stems from the collection of dietary data using a standardised 24-HDR in the whole cohort(Reference Slimani and Valsta57); thus, the results are easily comparable across the countries. Nevertheless, some limitations were encountered. As not all the EPIC cohorts are population based, these findings cannot be extrapolated to the general population of each region(Reference Slimani, Fahey and Welch28). A relevant weakness is the likely underestimation of real flavonoid intake, due to the unknown food flavonoid composition data (10 % of data in our FCDB and thearubigin data), and the omission of some food items (such as coffee substitutes) and herb/plant supplement intakes in the present analysis (which are up to 5 % of the population in Denmark, the highest supplement consuming country)(Reference Skeie, Braaten and Hjartaker58). Another limitation is the use of a single 24-HDR, which is less likely to reflect true usual individual diet. However, analyses were weighted by season and weekday of dietary recall and 24-HDR is a useful method to describe the average dietary intake of a group, particularly when estimated from a large number of subjects(Reference Willet59).
In summary, the data obtained in the present study show total flavonoid intake among the twenty-six EPIC participating centres in ten European countries. We also describe the major dietary flavonoid class contributors and the main food sources that differentiated the MED and non-MED regions. There was no significant difference in total flavonoid intake between MED and non-MED countries, although there were differences in flavonoid classes and their food sources between both regions. Significant socio-demographic and lifestyle factors related to total flavonoid intakes were also highlighted by European region. These descriptive data should be of value in the future as a first step in dietary flavonoid assessment and their potential role in health and disease.
Acknowledgements
The authors declare no conflict of interest. The present work was carried out with the financial support of the European Commission: Public Health and Consumer Protection Directorate 1993 to 2004; Research Directorate-General 2005; Ligue contre le Cancer, Institut Gustave Roussy, Mutuelle Générale de l'Education Nationale, Institut National de la Santé et de la Recherche Médicale (INSERM) (France); German Cancer Aid; German Cancer Research Center; German Federal Ministry of Education and Research; Danish Cancer Society; the Spanish Ministry of Health (ISCIII RETICC RD06/0020); Spanish Regional Governments of Andalusia, Asturias, Basque Country, Murcia (No. 6236) and Navarra and the Catalan Institute of Oncology; Cancer Research UK; Medical Research Council, UK; the Stroke Association, UK; British Heart Foundation; Department of Health, UK; Food Standards Agency, UK; the Wellcome Trust, UK; the Hellenic Health Foundation and the Stavros Niarchos Foundation; Italian Association for Research on Cancer; Compagnia San Paolo, Italy; Dutch Ministry of Public Health, Welfare and Sports; Dutch Ministry of Health; Dutch Prevention Funds; LK Research Funds; Dutch ZON (Zorg Onderzoek Nederland); World Cancer Research Fund (WCRF); Statistics Netherlands (The Netherlands); Swedish Cancer Society; Swedish Scientific Council; Regional Government of Skane, Sweden; Nordforsk – Center of Excellence Programme; some authors are partners of Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS), a network of excellence of the 6th Framework Programme of the European Commission. R. Z.-R. is thankful for a postdoctoral ‘Sara Borrell’ programme (CD09/00133) from the MEC and Instituto de Salud Carlos III. The author contributions are as follows: R. Z.-R. and C. A. G. designed the research; R. Z.-R. and V. K. conducted the research; R. Z.-R. and L. L.-B. performed the statistical analysis; R. Z.-R. wrote the manuscript. All authors read, critically reviewed and approved the final manuscript.
