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Ferulic acid and its derivatives modulate nitric oxide balance in HUVEC cells

Published online by Cambridge University Press:  11 December 2017

M. Le Sayec ,
G. Serreli ,
E. Thou ,
C. Lacour ,
J.P.E. Spencer  and
G. Corona
M. Le Sayec
Affiliation:
Health Sciences Research Centre, Life Sciences Department, Whitelands College, University of Roehampton, London, UK
G. Serreli
Affiliation:
Health Sciences Research Centre, Life Sciences Department, Whitelands College, University of Roehampton, London, UK
E. Thou
Affiliation:
Health Sciences Research Centre, Life Sciences Department, Whitelands College, University of Roehampton, London, UK
C. Lacour
Affiliation:
Health Sciences Research Centre, Life Sciences Department, Whitelands College, University of Roehampton, London, UK
J.P.E. Spencer
Affiliation:
Molecular Nutrition Group, School of Chemistry, Food and Pharmacy, University of Reading, Reading RG6 6AP, UK
G. Corona
Affiliation:
Health Sciences Research Centre, Life Sciences Department, Whitelands College, University of Roehampton, London, UK
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Abstract

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Nitric oxide (NO) is an important vasodilator involved in the regulation of vascular homeostasis, and plays a crucial role in maintaining a normal endothelial function( Reference Leong 1 ). Dietary phenolic acids such as ferulic acid can improve vascular function( Reference Vauzour 2 ), through maintenance of local levels of NO. Thus the molecular events involved are not fully understood( Reference Corona 3 ) and further mechanistic investigations are required. The aim of this study is to understand the precise cellular mechanisms by which ferulic acid and its metabolites maintain healthy vascular function through modulation of the NO pathway.

Primary Human Umbilical Vein Endothelial Cells were exposed to ferulic acid, isoferulic acid, hydroferulic acid, ferulic acid 4-O-glucuronide, isoferulic Acid 3-O-Sulfate and Dihydroferulic acid 4-O-glucuronide (1μM) for 24 h or 2 h. Apocynin and Nω-Nitro-L-arginine (L-NNA) were used as additional controls. Superoxide production, cyclic GMP cGMP) levels, and Akt1 activation were determined. The statistical analysis of the data (at least 4 biological replicates) was conducted by one-way ANOVA followed by a Tukey post-hoc t-test using the Graphpad 7 software.

* = p < 0·05, ** = p < 0·01, *** = p < 0·001 vs. control

Similarly to apocynin, all compounds tested showed the ability to enhance NO levels, measured as production of cGMP, and significantly decrease superoxide production, Protein expression results obtained through western blotting showed that the treatment with ferulic acid, isoferulic acid, hydroferulic acid and isoferulic Acid 3-O-Sulfate was also able to significantly increase Akt1 activation, measured as the ratio of the phosphorylated (Ser 473) / total protein. Our results indicated that all the tested ferulic acid metabolites can modulated NO balance, measured as cGMP production, by decreasing its degradation (via reduced superoxide formation), however the glucuronide-conjugated metabolites are not able to significantly enhance NO production through the Akt1/eNOS pathway.

This work was supported by the BBSRC (BB/M002802/1)

References

1. Leong, XF et al. (2015) Biomed Res Int May (3). 528757.Google Scholar
2. Vauzour, D et al. (2010) Nutrients (2): 1106–1131.Google Scholar
3. Corona, G, et al. (2014) Nutrition and Aging 2(2–3): 125–132.CrossRefGoogle Scholar