Biofabrication practices in Biodesign commonly rely on preformed cultures, standardized strains, and established protocols, often without critically addressing their provenance and ecological implications. This tension is particularly evident in SCOBY cultivation, where microbial cultures are frequently exchanged as fragments with uncertain origin. In response, and informed by Regenerative Ecologies frameworks, that advocate for ecological literacy and situated engagement with living systems, this contribution addresses how SCOBY can be grown ‘from scratch’. The investigation draws on a doctoral study engaging with acetic fermentation practices in Thailand and Germany, including apprenticeships, foraging, and solo experimentation with vernacular techniques. These experiences demonstrated that SCOBY can emerge from situated ecologies, highlighting the adaptive and relational nature of this microbial process. Building on these insights, this paper introduces an open-source method enabling practitioners to grow SCOBYs through engagement with situated plants and microbes. From these insights, Situated Growing Design is proposed as an emerging trajectory and practical approach for translating regenerative aspirations into growing material practices that foster closer engagements with bioregional configurations.