INTRODUCTION
Ascension Island is one of the remotest volcanic islands worldwide, located 07°57′ south of the Equator, near the Mid-Atlantic Ridge, about 1600 km from West Africa and 2400 km from South America. St Helena Island is the nearest island to Ascension, about 1130 km to the south-east.
The coastline of Ascension varies, ranging from sea cliffs and steep slopes (mainly eastern coasts) to sandy beaches and smooth sublittoral areas and rocky beaches (mainly western coasts), thus creating several different types of coastal habitats for various seaweed communities (Price & John, Reference Price and John1980). However, the most important factor governing seaweed development in Ascension is the presence of the marauding blackfish (Melichthys niger Bloch, 1786), the most dominant fish species around the coast and seemingly mainly responsible for the paucity of the island's benthic algal flora (Price & John, Reference Price and John1979, Reference Price and John1980).
Only a few studies regarding the marine seaweed flora of Ascension Island have been carried out previously. Osbeck (Reference Osbeck1757, Reference Osbeck and Koppe1765, Reference Osbeck and White1771) was the first to report marine algae from Ascension, based on drift specimens. In the 19th Century sparse records were provided by Bory (Reference Bory de Saint-Vincent and Duperrey1829) and Askenasy (Reference Askenasy and Engler1888), while about a century later a single green seaweed (Valonia sp.) was reported from the inland saline pools of the island (Chace & Manning, Reference Chace and Manning1972). Finally, a more detailed survey of Ascension's seaweeds was performed in October and November 1976 by Price & John (Reference Price and John1979, Reference Price and John1980). Taking into account their original work, additional records from Ascension Island were included in the series of ‘Seaweeds of the western coast of tropical Africa and adjacent islands: a critical assessment’ (Lawson & Price, Reference Lawson and Price1969; Price et al., Reference Price, John and Lawson1978, Reference Price, John and Lawson1986, Reference Price, John and Lawson1988, Reference Price, John and Lawson1992; John et al., Reference John, Price, Maggs and Lawson1979, Reference John, Lawson, Price, Prud'homme van Reine and Woelkerling1994; Lawson et al., Reference Lawson, Woelkerling, Price, Prud'homme van Reine and John1995; Woelkerling et al., Reference Woelkerling, Lawson, Price, John and Prud'homme van Reine1998) as well as in the updated catalogue of seaweeds of the western coast of Africa and adjacent islands (John et al., Reference John, Prud'homme van Reine, Lawson, Kostermans and Price2004)
After 36 years since the seaweed collections of Price & John (Reference Price and John1980) at Ascension shores, we conducted an extensive seaweed survey in the island's coastal environment, aiming to update the knowledge about Ascension marine benthic macroalgal flora. Older records from the island are also covered, providing a comprehensive check list of the marine seaweeds of Ascension Island.
MATERIALS AND METHODS
The seaweed flora of Ascension Island was investigated during a one-week period in February 2011 and a two-and-a-half-week period in September 2012. Several rocky sites were chosen along the island's shores (Table 1), from the eulittoral zone down to 38 m depth. Similar to previous work by Price & John (Reference Price and John1980) we sampled seaweeds in various types of coastal habitat: rock pools (Catherine Point, Collyer Point, Shelly Beach, Wideawake Plains); blow-hole areas (Catherine Point); turtle ponds (Georgetown); midlittoral rocks and beach rocks (Pillar Bay, Collyer Point, Clarkes Beach); the outfall channel from the island's main power station (Derby wreck); and sublittoral rocks (English Bay, Comfortless Cove, Sudan wreck site, Derby wreck site, Horseshoe Reef, Red Rock Cave, and a sublittoral reef west of Georgetown).
Seaweed taxa recorded in inshore waters of Ascension Island, based both on our collections and previous known literature. Taxa are listed alphabetically for each group (Ulvophyceae, Phaeophyceae and Rhodophyta), in order to make their detection easier. Synonyms used in previous publications are also given. Superscript numbers in taxa refer to notes. The exact location where each taxon was collected during our samplings as well as habitat details are provided (1, Catherine Point; 2, Clarkes Beach; 3, Collyer Point; 4, Comfortless Cove; 5, Derby wreck (in English Bay); 6, English Bay; 7, Georgetown; 8, Horseshoe Reef; 9, Pillar Bay; 10, Red Rock Cave; 11, Shelly Beach; 12, Sublittoral reef west of Georgetown; 13, Sudan wreck; 14, Wideawake Plains).
a, originally described from Ascension Island as Fucus lendigerum Linnaeus (Reference Linnaeus1753), based on drift material collected by Osbeck (Reference Osbeck1757, Reference Osbeck and Koppe1765, Reference Osbeck and White1771). However, neither the following phycologists visiting the island nor we have managed to detect the species, and we cite this species as taxon inquirendum;
b, based on Perrone et al. (Reference Perrone, Cecere and Furnari2006), this species should be treated as taxon inquirendum, since its neotype and other herbarium specimens studied probably correspond to different species;
c, only the tetrasporophytic stage found: Falkenbergia hillebrandii (Bornet) Falkenberg;
d, according to Won et al. (Reference Won, Cho and Fredericq2009) specimens identified using the ‘traditional’ concept for Centroceras clavulatum usually fall into other species. The actual C. clavulatum is restricted to the Pacific Ocean and thus the Price & John (Reference Price and John1980) record probably corresponds to another species of the genus, possibly C. gasparrinii;
e, according to the phylogenetic study by Cho et al. (Reference Cho, Boo, Hommersand, Maggs, McIvor and Fredericq2008) the ‘Ceramium flaccidum’ complex accommodates several Gayliella species. Thus, the single record of C. flaccidum by John et al. (Reference John, Prud'homme van Reine, Lawson, Kostermans and Price2004) requires further investigation.
Seaweeds were collected by snorkelling and SCUBA-diving through the destructive method (scraping the macroalgae off the substratum). Underwater photographs were taken using an Olympus PTWC-01 camera with PTDP-EP05 housing. The material collected was preserved in 4% buffered formalin/seawater and/or mounted on Bristol paper, pressed, air dried and prepared as herbarium specimens and silica-gel samples.
Specimens were studied in the laboratory of the Ascension Conservation Centre, under dissecting or compound microscopes. When necessary, they were sectioned manually with a razor blade. Species were identified down to the lowest possible taxonomic level. In several cases permanent material was prepared as microscope slides using Karo™ corn syrup (ACH Food Companies, Memphis, TN, USA).
Herbarium specimens have been deposited in the herbarium at the University of Aberdeen (School of Biological Sciences). For present-day taxonomic and nomenclatural opinions the following on-line taxonomic databases were consulted: Index Nominum Algarum (Silva, Reference Silva2014) and AlgaeBase (Guiry & Guiry, Reference Guiry and Guiry2014).
Given the limited time and logistic constraints at this remote island, inevitably leading to a limited coverage of the smaller representatives of the flora, collections of seaweed specimens were supplemented by collections of substratum samples stored in sterile tubes during the survey dives. Following return to Europe and based upon a protocol developed for a similar study in the Juan Fernandez Islands (Müller & Ramirez, Reference Müller and Ramirez1994), these samples were incubated in Provasoli-enriched seawater (Starr & Zeikus, Reference Starr and Zeikus1993) under white fluorescent light (12 h day: 12 h night) and a temperature of 18°C, which is slightly below the lower limit of the sea surface temperature present around Ascension. Over the next roughly three months, they were monitored for algal outgrowth, from which uni-algal isolates were made. Isolates were characterized and identified morphologically using a Zeiss PrimoVert inverted microscope and a Zeiss Axio Imager.D2 compound microscope, and by DNA sequencing and comparison with published data. The isolates have been deposited in the Culture Collection of Algae and Protozoa (CCAP, Oban).
DNA extractions were performed on four of the cultured isolates using CTAB buffer as described previously (Gachon et al., Reference Gachon, Strittmatter, Müller, Kleinteich and Küpper2009), or by using the GeneJET plant DNA kit (ThermoScientific; Cat. No. K0791) and following the manufacturer's instructions. Polymerase chain reactions (PCRs) were performed using specific primers for the internal transcribed spacer (ITS) and the small subunit rDNA (SSU). For ITS amplification, the primer pairs PI (Tai et al., Reference Tai, Lindstrom and Saunders2001) and KIRI (Lane et al., Reference Lane, Mayes, Druehl and Saunders2006), or ITS1 and ITS4 (White et al., Reference White, Bruns, Lee, Taylor, Innis, Gelfand, Sninsky and White1990) were used. SSU fragment amplification was achieved using the primer pair NS1 and NS4 (White et al., Reference White, Bruns, Lee, Taylor, Innis, Gelfand, Sninsky and White1990). Table 2 gives details of the primer sequences and some of the PCR conditions used. PCR was carried out with an initial denaturation at 94°C for 5 min, followed by 40 cycles of amplification consisting of denaturation at 94°C for 30 s, then annealing and extension steps as shown in Table 2. The 40 cycles were followed by a final extension at 72°C for 5 min. PCR amplification was performed using BIOTAQ™ DNA Polymerase (5 units μl−1; Bioline) and following the manufacturer's instructions while incorporating 1 μl of template DNA. PCR products were electrophoresed on 1.2% (w/v) agarose gels which were stained in GelRed nucleic acid stain and resulting DNA bands were visualized using a gel imager. Products of interest were sequenced by sending samples to either MWG Eurofins or Source Bioscience.
Sequences of primers used in polymerase chain reaction (PCR) for the amplification of partial nuclear ribosomal internal transcribed spacer and small subunit rDNA genes of 4 algal isolates (Table 3) from Ascension Island. Also detailed are the annealing and extension steps, along with the expected PCR product size.
The alignment of each DNA sequence returned was conducted using the BioEdit Sequence Alignment Editor™ (Hall, Reference Hall1999). For identifying taxa, sequences were compared to published data by means of NCBI BLAST searches (Altschul et al., Reference Altschul, Madden, Schäffer, Zhang, Zhang, Miller and Lipman1997).
RESULTS
In total, 82 marine macroalgae in species and infraspecific level were identified from our samplings (Table 1): 18 green algae (Ulvophyceae); 15 brown algae (Phaeophyceae); and 49 red algae (Rhodophyta). In addition, four taxa belonging to the genera Aglaothamnion, Corallophila, Lithophyllum and Rhodymenia were given under their generic names as identification to species level would require collection of fertile material and none of the material collected contained reproductive structures.
Among our results, 38 species and infraspecific taxa are reported for the first time from Ascension Island: seven green, three brown and 28 red macroalgae, raising the total number of seaweeds recorded in Ascension so far to 112 taxa in species and infraspecific level (Table 1).
Culturing substratum samples and monitoring algal outgrowth resulted in the isolation of four algal species which were further characterized by sequencing their partial SSU–ITS genes (Table 3). Two of them were confirmed (by both morphological identification and sequence homologies) to be Ulvella leptochaete and Stylonema alsidii respectively, of which U. leptochaete was not detected in our field samplings. Sphacelaria rigidula was identified morphologically (based on the presence of propagules) with a closest SSU sequence homology with Sphacelaria sp. UTEX LB800. Finally, some ambiguity remains about the Ulva isolate (Table 3). Based on classical taxonomy it was close to U. clathrata but the SSU was 99% similar to U. pertusa and U. lactuca. For ITS, however, the best BLAST hits (90% identity) were U. prolifera, U. erecta, U. taeniata, Ulva sp., and the two public sequences of U. clathrata showed only 88 and 87% identity. The identity of our isolate will have to be resolved in the context of currently ongoing taxonomic revisions of this genus.
Live isolates of 4 algal taxa included in the present study.
DISCUSSION
Floristics
Seventy-four species and infraspecific taxa had already been reported from Ascension Island in previous studies (Lawson & Price, Reference Lawson and Price1969; Price et al., Reference Price, John and Lawson1978, Reference Price, John and Lawson1986, Reference Price, John and Lawson1988, Reference Price, John and Lawson1992; John et al., Reference John, Price, Maggs and Lawson1979, Reference John, Prud'homme van Reine, Lawson, Kostermans and Price1994, Reference Lane, Mayes, Druehl and Saunders2004; Price & John, Reference Price and John1979, Reference Price and John1980; Lawson et al., Reference Lawson, Woelkerling, Price, Prud'homme van Reine and John1995; Woelkerling et al., Reference Woelkerling, Lawson, Price, John and Prud'homme van Reine1998). Based on our collections, 38 species and infraspecific taxa are added to the Ascension seaweed flora, raising the total number of recorded seaweeds so far to 112 taxa. As a result, the benthic flora of Ascension Island is richer than that of neighbouring St Helena, where only 47 species are known (Bolton et al., Reference Bolton, De Clerck, John, Decker, Griffiths, Prochazka, Ras and Whitfield2003). However, this difference may reflect the fewer studies conducted in St Helena regarding seaweeds.
Among our new records, rare species of interest include the green alga Cladophora corallicola and the red algae Acanthophora ramulosa, Champia cf. puertoricensis, Lophosiphonia adhaerens and Laurencia caduciramulosa. The latter might correspond to a recent introduction to Ascension Island, since it was reported only recently from the Atlantic Ocean: Brazil, Cuba and the Canary Islands (Cassano et al., Reference Cassano, Gil-Rodríguez, Sentíes and Fujii2008; Senties et al., Reference Senties, Areces, Diaz-Larrea and Fujii2010).
Our new records of Erythrotrichia bertholdii, Hydrolithon cruciatum and Vickersia baccata for Ascension, all known from the north-east and subtropical Atlantic Ocean and the Mediterranean Sea (Tsiamis et al., Reference Tsiamis, Montesanto, Panayotidis and Katsaros2011; Guiry & Guiry, Reference Guiry and Guiry2014) extend their distribution range to the tropical Atlantic Ocean. However, the vast majority of the recorded Ascension seaweeds are common in the tropical Atlantic Ocean. The algal flora of Ascension Island has features in common with those of both the eastern and western parts of the tropical and subtropical Atlantic Ocean (John et al., Reference John, Prud'homme van Reine, Lawson, Kostermans and Price2004; Wynne, Reference Wynne2011). Only a few species occurring in Ascension Island are absent from the western tropical and subtropical Atlantic Ocean, such as the red algae Herposiphonia brachyclados, Laurencia brachyclados, Lophosiphonia adhaerens and Rhodymenia holmesii. On the other hand, some red algae found in Ascension, such as Champia cf. puertoricensis, Diplothamnion tetrastichum, Lomentaria corallicola and Monosporus indicus, are lacking from the western coasts of Africa.
Most of the algal species were encountered on sublittoral rocks, holes and crevices or in rock pools. A number of 31 taxa (36% of our collections) were found exclusively in the sublittoral zone, pointing out the importance of carrying out SCUBA diving-based collections in poorly explored areas.
The present study attempted to complement the findings of field-collected samples by culturing portions of substratum collected during dives followed by identification of any isolates obtained by means of light microscopes and molecular analyses. Some success was achieved since one of the four taxa isolated (Ulvella leptochaete) was not discovered in field-collected samples and was identified based on morphological and molecular analysis (Table 3). This approach has proven particularly rewarding in remote locations where time in the field and local laboratory facilities may be limited, for example in the Juan Fernandez Islands (Müller & Ramirez, Reference Müller and Ramirez1994). Besides the green algae Ulva clathrata and Ulvella leptochaete, the red alga Stylonema alsidii and the brown alga Sphacelaria rigidula, we have generated numerous isolates of small multicellular algae whose identification is underway.
Overall marine vegetation
The marine vegetation around Ascension Island seems unchanged since the Price & John (Reference Price and John1979, Reference Price and John1980) visit in 1976. As pointed out by the latter authors, sublittoral marine vegetation seems absent at first sight. Seagrasses do not exist, probably due to strong wave exposure occurring on the sandy beaches. When it comes to the rocky bottom, the only evident dominant seaweeds covering the sublittoral seafloor are the non-geniculate coralline red algae, which seem to remain tolerant to heavy grazing by the blackfish that prevail around Ascension. These corallines cover large surfaces and constitute crucial structuring elements to the seabed communities and dominate the underwater seascape (see also Price & John, Reference Price and John1979, Reference Price and John1980). They either form tower-like, reef-forming structures or loose piles on the seabed (maerl–rhodoliths). Most of them correspond probably to Lithophyllum species (Athanasios Athanasiadis, personal communication) and further investigations are underway to identify the crustose coralline algae at species-level. Finally, conspicuous cyanobacteria can be found growing on coralline formations, which apparently are not grazed by the blackfish.
However, a closer look around Ascension's sublittoral shores reveals the presence of inconspicuous non-coralline seaweeds (see Table 1), with diminutive and repent habit creating turf-like mats, an adaptation to the strong grazing pressure by the blackfish, while in crevices, where access for the blackfish is limited, more flourishing algal communities can be encountered, such as Padina gymnospora and Cladophora spp.
Far more luxuriant and extensive seaweed populations can be found in shallower waters, where access for the blackfish is restricted. Midlittoral rocks and beach rocks, rock pools (including the turtle pond), blow-hole areas and the outfall channel host the most abundant and diverse seaweed clumps in the island, as noted also by Price & John (Reference Price and John1979, Reference Price and John1980), including various seaweeds such as Chaetomorpha antennina, Codium taylorii, Asteronema breviarticulatum, Chnoospora minima, Colpomenia sinuosa, Levringia brasiliensis, Palisada perforata and many others (see Table 1).
Finally, marine vegetation surveys did not reveal any major differences between the two samplings seasons (September and February), possibly due to the small seasonal variation in sea temperature: 23–25°C water temperature during the cold season and up to 28°C during the warm season (Shallow Marine Surveys Group, Stanley, Falkland Islands, unpublished personal communication).
CONCLUSION
Our knowledge about the marine algal flora of Ascension is increased by the addition of 38 species and infraspecific taxa as a result of the present study, and the number of taxa now recorded for the island is 112. Still, there is no doubt that this number is an underestimation, since most parts of the island, and also, in particular the deeper parts of the euphotic benthos, remain unexplored. Therefore, additional surveys are crucial for further exploration of the marine benthic flora of Ascension.
ACKNOWLEDGEMENTS
We are grateful to Nick John, Jimmy Young and Caroline Yon (all in Ascension Island) for sampling assistance. We would also like to thank Konstantinos Kondylis for help with the DNA amplification of macroalgal isolates. We are grateful to the Shallow Marine Surveys Group and the South Atlantic Environmental Research Institute for organizing the expedition. We are also very grateful to the Ascension Island Government, the members of staff at the Conservation Centre and Ascension Island Dive Club for their cooperation, accommodation and hospitality. Finally we are grateful to British Forces South Atlantic Islands for their logistic support.
FINANCIAL SUPPORT
The MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) is gratefully acknowledged for supporting F.C.K. The funding for this work came from a grant to the Shallow Marine Surveys Group from the Darwin Initiative (EIDCF012).
