Introduction
Most species of large carnivores are threatened as a result of their large territorial requirements, their naturally low densities and direct persecution by humans (Noss et al., Reference Noss, Quigley, Hornocker, Merrill and Paquet1996; Woodroffe & Ginsberg, Reference Woodroffe and Ginsberg1998). Knowledge of the presence of carnivores in human-dominated landscapes constitutes the basis for determining the conservation status of these species (Karanth & Nichols, Reference Chalukian2002; Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002). However, their secretive behaviour and low densities make research on, and monitoring of, large carnivores difficult (Karanth & Chellam, Reference Karanth and Chellam2009). Indirect evidence of animal presence (e.g. tracks and scats) is a valuable resource for surveying the distribution of secretive species (Wemmer et al., Reference Wemmer, Kunz, Lundie-Jenkins, McShea, Wilson, Cole, Nichols, Rudran and Foster1996; Karanth & Nichols, Reference Karanth and Nichols2002), and sign and questionnaire surveys are commonly used to determine the presence of large carnivores (McNab & Polisar, Reference McNab, Polisar, Medellín, Equihua, Chetkiewicz, Crawshaw, Rabinowitz and Redford2002; Altrichter et al., Reference Altrichter, Boaglio and Perovic2006; Carroll & Miquelle, Reference Carroll and Miquelle2006). However, limited funds and personnel often make it difficult to obtain this type of data across extensive areas and over long periods of time (Karanth & Nichols, Reference Karanth and Nichols2002; Danielsen et al., Reference Danielsen, Burgess and Balmford2005), and it is even more challenging when the search involves different countries with different cultures and languages.
Locally-based participatory monitoring programmes have demonstrated potential for surveying large areas and are particularly valuable in developing countries where the involvement of local volunteers may prompt awareness and management interventions (Danielsen et al., Reference Danielsen, Mendoza, Tagtag, Alviola, Balete and Enghoff2003, Reference Danielsen, Mendoza, Tagtag, Alviola, Balete and Jensen2007). In spite of relatively low cost and the possibility of covering large areas, participatory monitoring networks require tools for motivating the volunteers and maintaining their interest (Bell et al., Reference Bell, Marzano, Cent, Kobierska, Podjed and Vandzinskaite2008). In developing countries, where resources are often limited and the culture of volunteering is not necessarily common or organized, simple, inexpensive and motivating methodologies are required to guarantee the success of participatory monitoring activities (Danielsen et al., Reference Danielsen, Mendoza, Tagtag, Alviola, Balete and Enghoff2003, Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009; Bell et al., Reference Bell, Marzano, Cent, Kobierska, Podjed and Vandzinskaite2008). Charismatic species have been important motivators for promoting local participation (Bowen-Jones & Entwistle, Reference Bowen-Jones and Entwistle2002; Gray & Kalpers, Reference Gray and Kalpers2005; Danielsen et al., Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009) and large carnivores are frequently selected as flagship species in conservation programmes, although mainly for fund-raising and educational purposes (Dalerum et al., Reference Dalerum, Somers, Kunkel and Cameron2008; Karanth & Chellam, Reference Karanth and Chellam2009). However, as these species are often perceived as a threat to human lives and livestock by local people, the utility of their image as flagship species where local support is needed has been disputed (Bowen-Jones & Entwistle, Reference Bowen-Jones and Entwistle2002).
The jaguar Panthera onca and puma Puma concolor are the largest carnivores of the threatened Upper Paraná Atlantic Forest on the tri-national border area formed by Argentina, Brazil and Paraguay (Myers et al., Reference Myers, Mittermeier, Mittermeier, da Fonseca and Kent2000; Fig. 1). The jaguar is a species of particular conservation concern in this region. In Argentina it is both an Endangered species (Díaz & Ojeda, Reference Díaz and Ojeda2000) and a National Natural Monument (Law #25,463), and it is categorized as Vulnerable in Brazil (Chiarello et al., Reference Chiarello, Aguiar, Cerqueira, Melo, Rodrigues, Silva, Machado, Drummond and Paglia2008) and as Critically Endangered in Paraguay (SEAM, 2010). Because of its large territorial demands and requirement for an adequate prey base, the jaguar has been identified as an umbrella species to design a biodiversity conservation landscape for the Upper Paraná Atlantic Forest in a tri-national conservation initiative (the Biodiversity Vision; Di Bitetti et al., Reference Di Bitetti, Placci and Dietz2003). In addition, two areas in this part of the Atlantic Forest were selected by a group of jaguar experts as significant areas for the conservation of the species (Jaguar Conservation Units; Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002). Therefore, studying and monitoring jaguars in the Upper Paraná Atlantic Forest were considered priorities for the species and for ecoregional conservation strategies (Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002; Di Bitetti et al., Reference Di Bitetti, Placci and Dietz2003).
Main protected areas and forest remnants in the core area of the Upper Paraná Atlantic Forest ecoregion, in which puma Puma concolor and jaguar Panthera onca presence was surveyed. The rectangle on the inset indicates the location of the main map in South America.
Here we discuss the use of locally based monitoring programmes as a tool for a large-scale survey, using as a case study a project in which large carnivores were used as flagship species to promote participation. We present an updated distribution map for pumas and jaguars in the Upper Paraná Atlantic Forest resulting from a collaborative effort of local people, professionals and institutions in Argentina, Brazil and Paraguay. We also assess the effect of habitat loss on the distribution of pumas and jaguars in this Forest and the advantages of local participation and collaborative work for monitoring large carnivores at a regional scale.
Study area
We conducted this study in the core areas for long-term conservation of the Upper Paraná Atlantic Forest identified by the Biodiversity Vision (Di Bitetti et al., Reference Di Bitetti, Placci and Dietz2003; Fig. 1). The Upper Paraná Atlantic Forest is the largest of the 15 ecoregions that comprise the South American Atlantic Forest. It was formerly characterized by 470,000 km2 of subtropical semi-deciduous rain forests but < 8% of its original forest cover remains (Di Bitetti et al., Reference Di Bitetti, Placci and Dietz2003; Fig. 1). This ecoregion contains high levels of biodiversity and a diversity of human cultures (Galindo-Leal & de Gusmão Câmara, Reference de Gusmão Câmara, Galindo-Leal and de Gusmão Câmara2003), with a high, unevenly distributed human density and diverse economic activities (Jacobsen, Reference Jacobsen, Galindo-Leal and de Gusmão Câmara2003). The Upper Paraná Atlantic Forest is distributed in eastern Paraguay, most of Misiones Province in Argentina and portions of southern Brazil (Fig. 1). The largest area of this forest is in Brazil but most large fragments (> 100 km2) are in eastern Paraguay and the Misiones Green Corridor in Argentina, a corridor connecting two large Brazilian protected areas (Di Bitetti et al., Reference Di Bitetti, Placci and Dietz2003; Fig. 1).
Methods
We used two monitoring approaches to survey the puma and jaguar in the Upper Paraná Atlantic Forest. In Paraguay, Argentina and the two Brazilian protected areas connected by the Misiones Green Corridor (Fig. 1) we implemented a locally based participatory monitoring network: the Jaguar Project Monitoring Network. Additionally, we used data gathered during studies of jaguars and pumas by LC, DS, KCA and FL in the Upper Paraná–Pontal do Paranapanema Region, the northern portion of the Brazilian Upper Paraná Atlantic Forest (Fig. 2).
Distribution of the data records (n = 2,666) collected by research groups in the Upper Paraná–Pontal do Paranapanema Region (UPPR) and by the Jaguar Project Monitoring Network (JPMN). Dashed lines indicate the surveyed areas estimated using the density of records (99% kernel density estimator; see text for details).
The Jaguar Project Monitoring Network
In October 2002 we held a workshop in Puerto Iguazú, Argentina, integrating researchers and stakeholders from the main institutions involved in the conservation and management of the Upper Paraná Atlantic Forest. In this workshop we defined the methodology and identified potential participants for constructing a network of volunteers to monitor the presence of pumas and jaguars. Between mid 2002 and 2008 we conducted 70 field training workshops on sampling techniques and data collection for people living or working in areas where large carnivores are potentially present. Initially most of the participants were park rangers and field biologists (both men and women) and members of local governmental and non-governmental institutions, who supported this initiative and provided data from protected areas and their surroundings. However, as each volunteer encouraged the integration of more people into the network, we included farmers, ranchers, forestry workers, army patrols and students, whose joint efforts allowed us to obtain information from many regions both within and beyond protected and forested areas.
We trained the participants to search for and collect track imprints and faeces of large carnivores following simple instructions: ‘to collect tracks wider than 6 cm with the impression of the heel pad and four toes, and faecal samples > 2 cm diameter with prey content (hair, bones, hoofs)’. The main objective was to ensure that collaborators would collect every potential presence sign of pumas or jaguars, without the need for species-specific sign recognition in the field. Additionally, we designed a simple and rapid methodology of data collection to allow participants to incorporate the monitoring as a routine activity in their normal work without demanding extra costs and time. We provided them with a kit containing instructions and supplies for making plaster moulds of tracks and to collect and store faecal samples (Appendix 1). The kit also contained easy-to-complete cards to record data about the samples (date, collector name, site, environment). For faeces sampling, volunteers used disposable gloves and labelled paper bags. Faecal samples were dried by the volunteers inside the paper bags and stored with silica gel (Amato et al., Reference Amato, Rabinowitz and Hunter2006). Volunteers also recorded sightings and livestock depredation reports following pre-designed questionnaires. A coordinator (CDA) periodically visited or contacted the volunteers to compile data and provide extra kit supplies.
All participants worked for free, with the only motivation being that of collaboration for the conservation and management of both carnivore species. To help maintain motivation we prepared and distributed bulletins to inform participants about the progress of the project and provide practical information (e.g. tips for searching for presence signs). We also produced and distributed complementary materials, such as a track identification book (De Angelo et al., Reference De Angelo, Paviolo, Di Blanco and Di Bitetti2008), to help improve the quality of data collected. Additionally, we organized a tri-national workshop once per year (Appendix 2) to discuss progress and future plans with volunteers.
Although data collection was by volunteers, data selection, species identification and final analysis were by professionals, using accurate techniques and conservative criteria to avoid potential false positives of species’ presence. To identify tracks we followed the protocol established by De Angelo et al. (Reference De Angelo, Paviolo and Di Bitetti2010), using multiple measurements combined with a discriminant function analysis (Appendix 3). We extracted a piece of the best-preserved faecal samples for DNA identification of species following methods developed by Haag et al. (Reference Haag, Santos, De Angelo, Srbek-Araujo, Sana and Morato2009; Appendix 3). We classified sightings and livestock depredation events by pumas or jaguars according to their reliability (high: direct detection by a reliable observer or with physical evidence; medium: indirect detection obtained by a reliable informer but without physical evidence; low: not able to establish reliability with confidence). We did not consider records of low reliability as presence data, and we only considered records with medium reliability if they came from regions with physical evidence of species' presence recorded in nearby areas (< 10 km). To these data we added presence data collected by research projects (e.g. photographs from camera traps; Paviolo et al., Reference Paviolo, De Angelo, Di Blanco and Di Bitetti2008, Reference Paviolo, Di Blanco, De Angelo and Di Bitetti2009) and information from governmental agencies (e.g. denunciations compiled and verified by the Ministry of Ecology of Misiones or the National Park Administration of Argentina).
Collaborative research in the Upper Paraná–Pontal do Paranapanema Region
Since the late 1990s various research groups have been gathering information on large predators in the Upper Paraná–Pontal do Paranapanema Region (Crawshaw, Reference Crawshaw, Morato, Rodrigues, Eizirik, Mangini, Azevedo and Marinho2006). The records of jaguar and puma presence collected between 2002 and 2008 by these research groups include tracks, camera-trap photographs, records of individuals captured or killed and radio-tracking data (Cullen et al., Reference Cullen, Abreu, Sana and Nava2005; Cullen, Reference Cullen2006; Abreu et al., Reference Abreu, Mellek, Lima and Cullen2009; Pró-Carnívoros, 2009; IPÊ, 2010).
Data interpretation
We determined the geographical coordinates of each record using the information provided by collectors, local maps and satellite images. Less accurate records, such as locations of low certainty, degraded faecal samples, older records and material that we could not accurately identify as being from pumas or jaguars were only used to estimate the size of the surveyed area. We used the kernel density estimator tool (Bayer, Reference Bayer2004) to estimate the area covered by our survey. Kernel density estimation is a non-parametric way of estimating the probability density function of a random variable, incorporating information about both spatial distribution and density, and is typically used in home-range studies (Worton, Reference Worton1989).
To construct distribution maps of each species we used all presence points obtained between July 2002 and July 2008 that were confidently identified as puma or jaguar and were precisely located. We examined the locations of both species in relation to native forest cover (using forest cover estimated by supervised classification of Landsat Satellite images from 2004 by De Angelo, Reference De Angelo2009, where native marshland habitat was included as native forest) and protected areas (using a compilation of protected areas maps for the Upper Paraná Atlantic Forest region from De Angelo, Reference De Angelo2009).
Results
Between 2002 and 2008 we trained c. 320 people to collect data on the presence of pumas and jaguars in the Jaguar Project Monitoring Network, representing 40 institutions (governmental, non-governmental and private) and individuals (farmers, students and others). Although most of the volunteers were park rangers (30% including governmental and private protected areas), many collaborators were personnel or owners of private properties/companies (14%, mainly timber and forestry companies), NGO members (12%), students (11%), researchers (10%, including biologists, anthropologists and forestry engineers), local government staff (8%), army or border security patrols (5%), tourism guides (5%) and farmers (4%). Volunteer participation in the network was dynamic and not all people were active throughout the 6 years but at least 100 volunteers participated for the entire monitoring period. This collaborative effort resulted in 2,667 records (Fig. 2): 33.2% from researchers working in the Upper Paraná–Pontal do Paranapanema Region and the remainder from the Jaguar Project Monitoring Network. The distribution of all records covered an area of 92,890 km2 (99% kernel, h = 13,000 m; Fig. 2) but most of the data were concentrated in 54,181 km2 (95% kernel, h = 13,000 m). The surveyed area included 86% of the protected areas of the Upper Paraná Atlantic Forest (Appendix 4) and covered 66.2% of the remaining forest, including 68% of fragments 100–1,000 km2 in size and seven of the eight largest forest fragments > 1,000 km2 (considering the Green Corridor divided by main roads into four large forest fragments; Fig. 2). Of the total data 61.2% of records were classified as reliable evidence of the presence of pumas or jaguars (Table 1).
Confirmed records of puma Puma concolor and jaguar Panthera onca in the Upper Paraná Atlantic Forest (Fig. 1) between July 2002 and July 2008.
1 Details of track and faecal sample identification in Haag et al. (Reference Haag, Santos, De Angelo, Srbek-Araujo, Sana and Morato2009), De Angelo et al. (Reference De Angelo, Paviolo and Di Bitetti2010) and Appendix 3
2 Includes skin and tissue samples, camera-trap photographs and poached animals
3 Data from various surveys by research groups in the Upper Paraná–Pontal do Paranapanema Region, in the northern Upper Paraná Atlantic Forest. It includes captured/removed individuals, poached individuals, tracks, sightings, camera-trap photographs and conflicts with cattle.
4 Telemetry locations were obtained from one puma and 10 radio-collared jaguars (details in Cullen, Reference Cullen2006).
5 Total without considering radio-tracking locations (total including radio-tracking locations in parentheses).
Records of pumas were more abundant than those of the jaguar, independently of the type of record (mean = 2.25 ± SE 0.63 times more abundant; Table 1), with the exception of radio-tracking records that were obtained from an unbalanced number of animals (one puma versus 10 jaguars monitored; Cullen, Reference Cullen2006). The puma was present in most of the study area except for those areas with high human disturbance (Fig. 3). The jaguar, in contrast, was concentrated mainly in the Green Corridor of Argentina–Brazil and in the largest forest fragments in Brazil and Paraguay (Fig. 3). In Brazil we recorded jaguars only in or near the largest protected areas (Fig. 3, Appendix 4): Morro do Diabo State Park, Ivinhema State Park, Ilha Grande National Park, Perobas Biological Reserve, Iguaçu National Park and Turvo State Park. In Paraguay the largest protected areas also contained most of the country’s jaguar records (Mbaracayú Natural Reserve, Morombí Private Reserve, Limoy and Itabó Biological Refuges and the southernmost record in Paraguay, in San Rafael Reserve Area for National Park, S 26°38′13.7″ W 55°39′42.1″; Fig. 3, Appendix 4). We recorded the southernmost jaguars in the entire Upper Paraná Atlantic Forest in Turvo State Park in Brazil (S 27°10′14.6″ W 53°51′4.9″) and in Moconá Provincial Park in Argentina (S27°9′33.4″ W 53°53′28.7″). Puma records extend further south in both Argentina and Brazil (Fig. 3).
(a) Puma and (b) jaguar distribution in the Upper Paraná Atlantic Forest as determined by collaborative and participatory monitoring between July 2002 and July 2008. Jaguar Conservation Units (Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002): (A) Green Corridor, (B) Upper Paraná–Pontal do Paranapanema Region. Note the numerous areas with jaguar records detected outside the Jaguar Conservation Units, particularly in Paraguay. Horizontal black arrows indicate the area of the Paraguayan Upper Paraná Atlantic Forest with large forest fragments that are poorly surveyed and where there is a high probability of the presence of both species. See caption to Fig. 2 for details of delimitation of the surveyed areas in the UPPR (Upper Paraná–Pontal do Paranapanema Region) and JPMN (Jaguar Project Monitoring Network).
We obtained most puma (83%) and jaguar (83%) records in areas covered by native forest or native marshland habitat, and we confirmed puma presence in more forest fragments than jaguars (Fig. 4). Both species occurred in all the larger fragments (> 100 km2) but < 2% of surveyed forest fragments < 10 km2 in area had evidence of the species. We detected pumas in a higher proportion of small and medium sized fragments than jaguars (Fig. 4) and the total area covered by fragments with confirmed puma presence (19,266 km2) was larger than the total area with confirmed presence of jaguars (16,585 km2). For both species these areas are < 25% of the total area surveyed.
Percentage of surveyed forest fragments with confirmed puma and jaguar presence, by fragment size categories (in parentheses: number of fragments with presence/total number of fragments in category included in the surveyed area).
We detected jaguars in seven large forest fragments (i.e. > 100 km2) outside the Jaguar Conservation Units defined for this region (Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002): Salto Encantado and Cuña Pirú provincial parks in Argentina, Morro do Diabo State Park in Brazil and five fragments in Paraguay (Fig. 3).
Discussion
Partnership for regional surveys of large carnivores
Participatory monitoring and collaboration among scientists allowed us to obtain and compile data on the distribution of two secretive species, the puma and jaguar, in most of the remnants of the Upper Paraná Atlantic Forest ecoregion. This survey had two important characteristics. One was the interaction between research groups from different countries, combining their local knowledge to understand patterns occurring at a regional scale. Partnership between researchers has produced useful results for jaguars and other species in other parts of the jaguar’s distribution (Grigione et al., Reference Grigione, Menke, López-González, List, Banda and Carrera2009) and at a continental scale (Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002).
The second characteristic was the participatory monitoring carried out by a wide network of locally based teams. Danielsen et al. (Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009) suggested a typology for monitoring programmes according to the degree of local participation. Following this typology the Jaguar Project Monitoring Network could be classified as ‘collaborative monitoring with external data interpretation’ because local people participated not only in data collection but also in the design of the monitoring, whereas data analyses and decision making were by professionals. Danielsen et al. (Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009) defined eight characteristics of the benefits and demands of participatory monitoring, and Table 2 summarizes these for the Jaguar Project Monitoring Network.
The implementation of the Jaguar Project Monitoring Network faced not only the challenges associated with the survey of a large area by people from different countries and cultures but also communication difficulties (e.g. isolated areas, difficult access by vehicle and no telephones). Nevertheless, it yielded a large amount of data and involved many local people, with no costs for participants and low costs for external institutions (Table 2). Considering Danielsen et al.’s (Reference Danielsen, Burgess and Balmford2005) summary, external cost investment in the Jaguar Project Monitoring Network (< USD 0.01 ha-1 year-1) is among the lowest costs estimated for similar participatory monitoring programmes (USD 0.01–0.13 ha-1 year-1) and much lower than the cost estimated for professional surveys (c. USD 3.6 ha-1 year-1; Danielsen et al., Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009; Table 2). Although professional surveys (e.g. with camera traps) may allow more detailed information to be obtained (e.g. density estimates), their application at broader scales may not only be more expensive but also practically infeasible or unsustainable for long periods of time. Locally based surveys have the advantage of being able to maintain permanent long-term monitoring in many areas simultaneously, which is the best survey technique for obtaining data in areas where large carnivores live at extremely low densities (Karanth & Nichols, Reference Karanth and Nichols2002).
Evaluation of the Jaguar Project Monitoring Network according to the main characteristics of monitoring schemes suggested by Danielsen et al. (Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009).
* Some examples of combined management interventions with the involvement of different parts of the Jaguar Project Monitoring Network, as communicated in the local, national and international press, can be found in Territorio Digital (2004, 2008) Mullen (Reference Mullen2006), La Nación (2007) and Misiones Online (2009)
The low cost associated with the Jaguar Project Monitoring Network was not only the result of a simple affordable methodology but also of the commitment of participants and the support received from many local governmental and non-governmental institutions that allowed the monitoring to be incorporated in the routine activities of their personnel. Volunteers showed great interest and enthusiasm for seeking evidence of large predators, and the subsequent recognition for their work from the coordinator and other volunteers promoted increased efforts. Enthusiasm, credit and desire for learning have been recognized as important drivers for maintaining interest amongst volunteers (Danielsen et al., Reference Danielsen, Mendoza, Tagtag, Alviola, Balete and Jensen2007; Bell et al., Reference Bell, Marzano, Cent, Kobierska, Podjed and Vandzinskaite2008). However, the powerful image of the species monitored was essential to enthuse and involve local people and institutions in the Network, and both species, but mainly the jaguar, were important motivators for people and institutions, and also contributed to the growth of the Network (e.g. people who spontaneously offered to join the Network because of their interest in jaguars). This demonstrated that with adequate motivation and methods, volunteer work is possible in developing countries even though volunteering is not as culturally common as in developed countries (Danielsen et al., Reference Danielsen, Mendoza, Tagtag, Alviola, Balete and Enghoff2003, Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009; Bell et al., Reference Bell, Marzano, Cent, Kobierska, Podjed and Vandzinskaite2008).
Comparable experiences of motivation were reported by Poulsen & Luanglath (Reference Poulsen and Luanglath2005) in participatory biodiversity monitoring in Laos and by park rangers monitoring gorilla Gorilla beringei beringei populations in Rwanda, Uganda and the Democratic Republic of Congo (Gray & Kalpers, Reference Gray and Kalpers2005). As in the Jaguar Project Monitoring Network, gorillas were used as flagship species to help maintain participants’ interest and motivation. Through the Network we showed that, despite their conflicting image for cattle ranchers and some local people (Conforti & Azevedo, Reference Conforti and Azevedo2003), large carnivores may have special value as flagship species when they have positive associations for the selected focal audience (Bowen-Jones & Entwistle, Reference Bowen-Jones and Entwistle2002; Dalerum et al., Reference Dalerum, Somers, Kunkel and Cameron2008). Although most participants in the Network had a positive relationship with jaguars some ranchers, who may be expected to have a negative relationship with the species, joined the network because of their concern for the conservation of felids. Although we did not put much effort into the recruitment of ranchers as volunteers, we believe that rancher-based local monitoring with adequate advice from professionals (i.e. agronomists, sociologists) and government involvement may prove a useful tool not only in monitoring predators but also in reducing human–predator conflicts.
In spite of low costs and strong local support, the Jaguar Project Monitoring Network demanded large and permanent coordination efforts (e.g. personal contact with volunteers and communication activities). Because the coordinator (CDA) could not commit himself to this endeavour for > 5 years and because funds were not secured for the long-term (the project was conceived and funded mostly by NGOs), the continuity of the coordination of the Network and the monitoring was not ensured, and finished in 2008. Discontinuous funding is a common problem for sustaining monitoring over long periods of time (Brashares & Sam, Reference Brashares and Sam2005; Poulsen & Luanglath, Reference Poulsen and Luanglath2005). The involvement of local institutions is essential to guarantee monitoring continuity over time without permanent external funds, enhancing the important role that involvement of local institutions has for participatory monitoring (Danielsen et al., Reference Danielsen, Burgess and Balmford2005; Gray & Kalpers, Reference Gray and Kalpers2005; Poulsen & Luanglath, Reference Poulsen and Luanglath2005).
Similar surveys have frequently left identification of records in charge of local people (e.g. using interviews; McNab & Polisar, Reference McNab, Polisar, Medellín, Equihua, Chetkiewicz, Crawshaw, Rabinowitz and Redford2002; Altrichter et al., Reference Altrichter, Boaglio and Perovic2006). However, jaguars have a conspicuous cultural value in local communities in the Upper Paraná Atlantic Forest (Conforti & Azevedo, Reference Conforti and Azevedo2003) and their strong image and the similarity of their signs with those of pumas may cause a bias towards overrating jaguar presence and to misidentify the signs of pumas as belonging to jaguars, as occurs with other large carnivores (Lynam, Reference Lynam, Medellín, Equihua, Chetkiewicz, Crawshaw, Rabinowitz and Redford2002). The use of physical evidence and precise identification methods reduces this problem but demands more professional involvement. However, professional participation may not only increase accuracy and precision but also result in a wider use of the data gathered (Table 2).
There are two other important aspects of local participation in monitoring biodiversity: its implications for promptness of decision making and its potential for enhancing local stakeholder capacity (Danielsen et al., Reference Danielsen, Burgess, Balmford, Donald, Funder and Jones2009). The Jaguar Project Monitoring Network had significant outcomes in both aspects (Table 2) but, most importantly, it promoted collaborative work in conservation and management of large felids at a regional scale through the involvement and training of people from different institutions, professions and countries. Since the creation of the Network complaints of potential conflicts (e.g. felid sightings in populated areas, cattle killed) are communicated not only to local authorities but also to Network members, favouring the intervention of many institutions. As a result, a protocol of action is being drawn up and will be implemented through the bi-national action plan for jaguars in the Argentina–Brazil Green Corridor. This plan incorporates not only this protocol but also the information and ideas collected in the various different meetings in which the Network volunteers participated (Table 2). Additionally, the information gathered proved useful for other regional conservation initiatives: an action plan for jaguars in Paraguay is in the initial stages and, in the Upper Paraná–Pontal do Paranapanema Region, a jaguar action plan is being developed (Table 2).
Jaguars and pumas in the Upper Paraná Atlantic Forest
Although many human pressures can affect the persistence of species, habitat destruction has particularly harmful effects on large carnivores that require extensive territories (Karanth & Chellam, Reference Karanth and Chellam2009). Pumas and jaguars in the Upper Paraná Atlantic Forest have been seriously affected by forest loss and fragmentation. A clear indication of this is that these species are almost exclusively associated with medium and large fragments of native forest in a region where most of the landscape has been transformed to anthropogenic land uses (Figs 3 & 4).
The puma still occupies most of its continental distribution but has disappeared or became rare in those areas with the highest human pressures (Sunquist & Sunquist, Reference Sunquist and Sunquist2002). In the Upper Paraná Atlantic Forest pumas are present in a significant proportion of the forest remnants, including many areas where the jaguar was not recorded (Fig. 3). However, fragments with puma presence represent < 25% of the surveyed area and we did not find evidence of pumas in areas with intensive agriculture or with high human presence (Figs 3 & 4). Therefore, although pumas have apparently suffered less range contraction than jaguars, habitat loss and fragmentation along with the impacts of intense logging and poaching have resulted in the decrease of puma populations in the Upper Paraná Atlantic Forest (Paviolo et al., Reference Paviolo, Di Blanco, De Angelo and Di Bitetti2009).
The continental distribution of the jaguar has contracted severely (Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002; Zeller, Reference Zeller2007). In Brazil, where c. 50% of the continental range of the jaguar persists, habitat loss is the most important cause of jaguar decline (Tôrres et al., Reference Tôrres, De Marco, Diniz Filho and Silveira2008). In the Brazilian Atlantic Forest habitat destruction has been high (de Gusmão Câmara, Reference de Gusmão Câmara, Galindo-Leal and de Gusmão Câmara2003), and Mazzolli (Reference Mazzolli2009) described a south-to-north reduction of jaguar distribution in coastal Atlantic Forest caused by habitat fragmentation and poaching of jaguars. In the Upper Paraná Atlantic Forest of Brazil the main forest fragments are within protected areas, and it is only in these areas where the jaguar persists (Fig. 3, Appendix 4). Habitat loss is probably the main reason for jaguar range contraction in the Brazilian Upper Paraná Atlantic Forest but poaching of jaguars and their prey is also affecting jaguars in this region (Conforti & Azevedo, Reference Conforti and Azevedo2003; Cullen et al., Reference Cullen, Abreu, Sana and Nava2005; Abreu et al., Reference Abreu, Mellek, Lima and Cullen2009). Reducing these threats is essential for jaguar survival (Cullen et al., Reference Cullen, Abreu, Sana and Nava2005) but habitat recovery and connectivity are necessary to increase dispersal between populations and to reduce the genetic loss that fragmentation is producing in this region (Haag et al., Reference Haag, Santos, Sana, Morato, Cullen and Crawshaw2010).
In Paraguay we also obtained most jaguar records from within protected areas that harbour the most extensive forest fragments. The largest jaguar population in eastern Paraguay is probably located in Mbaracayú Nature Reserve and surrounding areas (Fig. 3). However, important forest remnants not surveyed by the Jaguar Project Monitoring Network persist in Canindeyú, Amambay and San Pedro departments, where we obtained informal data of puma and jaguar presence (Fig. 3). These areas could be important for maintenance of the connectivity among jaguar populations of the Atlantic Forest and chacoan and cerrado jaguar populations in western Paraguay. Although 30 years ago the Paraguayan Upper Paraná Atlantic Forest was a vast continuous forest it has suffered recent and rapid conversion (Huang et al., Reference Huang, Kim, Altstatt, Townshend, Davis and Song2007). Many of the areas with jaguar presence in Paraguay correspond with recently reduced and isolated fragments, and many of these isolated and small jaguar populations will probably disappear in the short-term. Although other pressures exist, habitat loss and fragmentation are also the main threats to the jaguar in eastern Paraguay.
In the Argentinian Upper Paraná Atlantic Forest jaguar records were concentrated in the Green Corridor (Fig. 4) where a large and continuous corridor of forest remains. Argentina has had the highest national rate of jaguar range contraction (Di Bitetti et al., Reference Di Bitetti, De Angelo, Paviolo, Schiaffino, Perovic, Brown, Martinez Ortiz, Acerbi and Corcuera2006), occurring in a south-to-north pattern. The last records of jaguars in Corrientes Province (south of Misiones province) date from the 1970s (Giraudo & Povedano, Reference Giraudo, Povedano, Galindo-Leal and de Gusmão Câmara2003) and the latest reliable records we obtained for southern Misiones date from the early 1990s. Since the mid 1990s the southernmost jaguar records in Misiones are in the central and eastern parts of the province (Giraudo & Povedano, Reference Giraudo, Povedano, Galindo-Leal and de Gusmão Câmara2003) where jaguars were detected by the Jaguar Project Monitoring Network (Fig. 3). These areas, and Turvo State Park in Brazil, are currently the southernmost limit of jaguar distribution (Sanderson et al., Reference Sanderson, Redford, Chetkiewicz, Medellin, Rabinowitz, Robinson and Taber2002; Zeller, Reference Zeller2007). Although habitat loss is affecting jaguar range in the Argentinian Upper Paraná Atlantic Forest, many areas with large forest fragments outside the Green Corridor harbour pumas but not jaguars (Fig. 3), suggesting that other factors have caused the disappearance of jaguars in these areas. A vast extent of the Argentinian Forest has been heavily logged and there is high poaching pressure on the jaguar and its prey, probably the main causes of a recent decline of jaguars in the Green Corridor (Paviolo et al., Reference Paviolo, De Angelo, Di Blanco and Di Bitetti2008). Although maintenance of forest cover is essential, reducing other human threats is critical for survival of the jaguar in this area (Paviolo et al., Reference Paviolo, De Angelo, Di Blanco and Di Bitetti2008).
The monitoring programme allowed us to detect both species in areas where there was no information available or where they were considered rare or locally extinct. The data have contributed not only to a more complete understanding of the effect of habitat loss on both species (e.g. through habitat suitability modelling, De Angelo et al., 2011, and genetic studies, Haag et al., Reference Haag, Santos, Sana, Morato, Cullen and Crawshaw2010) but is also helping in conservation and management actions (Table 2). An updated and more detailed definition of the Jaguar Conservation Units (Fig. 3) will help to improve local, regional and continental conservation plans (Rabinowitz & Zeller, Reference Rabinowitz and Zeller2010). A re-evaluation of the conservation landscape designed by Di Bitetti et al. (Reference Di Bitetti, Placci and Dietz2003) for the Upper Paraná Atlantic Forest is in progress, and this is another important outcome for which the data collected in this participatory survey were utilized.
Acknowledgements
We are very grateful to all the volunteers, collaborators and institutions who participated in the Jaguar Project Monitoring Network, particularly to C. Boiero, P. Crawshaw Jr, Y. Di Blanco, E. Eizirik, A. García, M. Gómez, E. Krauczuk, M. Jaramillo, R. Melzew, E. Pizzio, G. Placci, M. Rinas, A. Rodrigues, K. Schiaffino, S. Welcz, the National Parks Administration (Argentina), the Ministry of Ecology of Misiones Province (Argentina), Itaipu Binacional (Paraguay/Brazil), Pro-Cosara (Paraguay), Instituto de Derecho y Economía Ambiental (Paraguay), and our hosting institutions. Financial support was provided by the National Research Council of Argentina (CONICET), Fundación Vida Silvestre Argentina, WWF–USA, WWF–International, WWF–Switzerland, WWF Education for Nature Program, Species Action Fund (WWF–US) and Lincoln Park Zoo.
Appendices
The appendices for this article are available online at http://journals.cambridge.org
Biographical sketches
The authors work on various aspects of the ecology, genetics, conservation and management of biodiversity in Argentina, Brazil and Paraguay. As scientists, managers or conservationists the authors belong to a variety of governmental and non-governmental institutions but share a common interest in biodiversity conservation, and are particularly concerned about the conservation of pumas and jaguars as keystone and umbrella species in the Atlantic Forest. In this study they combined their efforts and expertise, taking advantage of their diverse capabilities to assess the population status of large felids from a regional perspective and undertake coordinated conservation actions.
