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Evaluation of cellular and antioxidant effects of casein hydrolysates

Published online by Cambridge University Press:  19 November 2010

N. Lahart ,
S. A. Aherne ,
D. O'Sullivan ,
R. J. FitzGerald  and
N. M. O'Brien
N. Lahart
Affiliation:
School of Food and Nutritional Sciences, University College Cork, Republic of Ireland
S. A. Aherne
Affiliation:
School of Food and Nutritional Sciences, University College Cork, Republic of Ireland
D. O'Sullivan
Affiliation:
Department of Life Sciences, University of Limerick, Republic of Ireland
R. J. FitzGerald
Affiliation:
Department of Life Sciences, University of Limerick, Republic of Ireland
N. M. O'Brien
Affiliation:
School of Food and Nutritional Sciences, University College Cork, Republic of Ireland
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Abstract

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Casein hydrolysates can influence numerous processes within the body, including gastrointestinal, immunological, neurological and nutritional responses(Reference Phelan, Aherne and FitzGerald1). Previously, we assessed eight different casein hydrolysates for potential bioactivity and found that these samples exerted varying effects on human Jurkat T cell viability, proliferation, antioxidant activity and cytokine production(Reference Phelan, Aherne and O'Sullivan2). Consequently, the casein hydrolysate that exerted the most bioactive effects was further hydrolysed to varying degrees and the subsequent hydrolysates were labelled B–E. Intact sodium caseinate was included as an unhydrolysed control (A). The aim of the present study was to assess if the bioactive effects of casein hydrolysates A–E were dependent on the extent (or degree) of hydrolysis.

Jurkat T cells were supplemented with 0.5% (v/v) casein hydrolysates B–E and intact sodium caseinate (A) for 24 h at 37°C. Cell viability was determined using the MTT assay (Roche Diagnostics, West Sussex, UK). Membrane integrity was assessed using the lactate dehydrogenase (LDH, EC 1.1.1.27) release assay. Cellular glutathione (GSH) content was measured by the method of Hissin and Hilf(Reference Hissin and Hilf3) and data were expressed as the percentage of untreated (control) cells.

CH: casein hydrolysate.

Values are means for four independent experiments.

* Significantly different from control (untreated) cells: ANOVA, followed by Dunnett's test; P<0.05.

Casein (A) and its hydrolysates B, C, D and F did not affect cell viability as determined by the MTT assay, which measures mitochondrial activity. Treatment with sample E for 24 h significantly (P<0.05) reduced the viability of Jurkat T cells. In terms of membrane integrity (LDH release), none of the casein hydrolysates induced adverse effects. When compared with control cells, samples B, C, D and F significantly increased (P<0.05) cellular GSH content. On the other hand, the presence of intact sodium caseinate (A) significantly reduced the GSH content of Jurkat cells. In conclusion, there is no relationship between the degree of hydrolysis of this casein hydrolysate and its bioactive effects on human Jurkat T cells.

This work was supported by the Food Institutional Research Measure (FIRM) as administered by the National Development Plan 2006–2011.

References

1.Phelan, M, Aherne, SA, FitzGerald, RJ et al. (2009) Int Dairy J 19, 643–654.CrossRefGoogle Scholar
2.Phelan, M, Aherne, SA, O'Sullivan, D et al. (2009) Int Dairy J 19, 279–285.CrossRefGoogle Scholar
3.Hissin, PJ & Hilf, R (1976) Anal Biochem 74, 214–226.CrossRefGoogle Scholar
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