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Differential cellular immune response in inductive and effectors sites after oral administration of quercetin

Published online by Cambridge University Press:  04 August 2011

E. M. Insani ,
A. C. Mignaqui ,
A. A. Pazos ,
M. E. Roux  and
N. H. Slobodianik
E. M. Insani
Affiliation:
Institute of Food Technology (INTA), Buenos Aires, Argentina
A. C. Mignaqui
Affiliation:
Institute of Food Technology (INTA), Buenos Aires, Argentina
A. A. Pazos
Affiliation:
Institute of Food Technology (INTA), Buenos Aires, Argentina
M. E. Roux
Affiliation:
Laboratory of Mucosal Immunity, Department of Cellular Biology, University of Buenos Aires, Buenos Aires, Argentina CONICET, Buenos Aires, Argentina
N. H. Slobodianik
Affiliation:
Department of Nutrition, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina
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Abstract

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The aim of this research was to compare the immune response after oral administration of quercetin(Reference Insani, Mignaqui and Pazos1) in mesenteric lymphoid nodes (MLN; inductive site) and lamina propria of intestinal villi (IV; effector site)(Reference Nagler-Anderson2).

An experimental model was used to evaluate the effect of protein malnutrition and allergic response. Weanling rats of Wistar strain were fed a protein-free diet until they lost 25% of their initial body weight. Re-feeding was performed by the administration of an experimental diet containing 20% casein as the only source of protein (Re-nourished group; R). Other experimental groups received this experimental diet plus quercetin (R+Q1 or R+Q2) (Q1=140 μg/kg body weight per d; Q2=280 μg/kg body weight per d; mean value), added to drinking water during 40 d. Three well-nourished groups were used as normal controls (C) which were fed with standard commercial diet or the same diet plus quercetin (C+Q1 and C+Q2). The MLN and small intestine were removed and properly processed, the last ones by Saint-Marie´s technique(Reference Saint Marie3). IgE+ B-cells were measured (Indirect Immunofluorescent Assay). The animal protocol was approved by the ethical committee of the University of Buenos Aires.

Results showed that in MLN R group presented higher % of IgE+ B-cells compared with C (42±2; 28±2) (P<0.0001). This value diminished to 17±2 in R+Q1, normalising the values under the normal control, C. With double dose of quercetin, R+Q2 was statistically lower than R but this dose resulted less efficient to diminish % of IgE+ B-cells (R+Q2=34±2) (Figs. 1 and 2).

Fig. 1. IgE+ B-cells in MLN.

Fig. 2. IgE+ B-cells in IV.

In IV, the effector site of the immune response, R group presented the highest number of IgE+ B-cells reaching to 165±7 (C 62±7; P<0.0001). In this case, double dose of quercetin was more efficient for lowering IgE+ B-cells compared with Q1 (R+Q, 2 71±6; R+Q1, 99±6).

In conclusion, quercetin intake presented differential effect for IgE+ B-cells related to inductive or effectors sites of immune response. These findings have to be taken into account to describe the effect of bioactive compound of nutritional interest.

References

1.Insani, EM, Mignaqui, AC, Pazos, AA et al. (2010) Proc Nutr Soc 69 (OCE3), E286.CrossRefGoogle Scholar
2.Nagler-Anderson, C (2001) Nat Rev/Immunol 1, 59–67.Google Scholar
3.Saint Marie, G (1962) J Histochem Cytochem 10, 250–256.CrossRefGoogle Scholar
Figure 0

Fig. 1. IgE+ B-cells in MLN.

Figure 1

Fig. 2. IgE+ B-cells in IV.