The increasing incidence of obesity is considered as a major health problem due to its co-morbidity of a number of diseases, including type 2 diabetes mellitus, CVD, and certain types of cancer(Reference Seidell1, Reference Pi-Sunyer2). Obesity is the result of a positive energy balance due to energy intake exceeding energy expenditure. In the system of body-weight regulation several pathways are involved and therefore weight management requires a multi-factorial approach(Reference Westerterp-Plantenga, Luscombe-Marsh, Lejeune, Diepvens, Nieuwenhuizen, Engelen, Deutz, Azzout-Marniche, Tome and Westerterp3). Recent findings suggest that a relatively elevated protein intake seems to play a role during weight loss as well as during weight maintenance thereafter(Reference Skov, Toubro, Ronn, Holm and Astrup4–Reference Clifton, Keogh and Noakes7). In addition to the protein-induced satiety that has been shown after a high-protein diet, protein-induced satiety has also been shown after a single meal(Reference Bertenshaw, Lluch and Yeomans8–Reference Halton and Hu10). Several studies on different types of protein affecting satiety have been executed(Reference Hall, Millward, Long and Morgan11–Reference Uhe, Collier and O'Dea16). The question remains, however, whether the larger satiating effects of high-protein meals hold for each specific type of protein.
Casein is a part of milk protein; it comprises 80 % of the protein content of bovine milk(Reference Miller, Witherly and Clark17). Casein is considered as a ‘slow’ protein because it coagulates in the stomach and delays gastric emptying(Reference Boirie, Dangin, Gachon, Vasson, Maubois and Beaufrère18). The slower digestion rate of casein results in smaller but prolonged increased postprandial plasma amino acid levels(Reference Boirie, Dangin, Gachon, Vasson, Maubois and Beaufrère18, Reference Dangin, Boirie, Garcia-Rodenas, Gachon, Fauquant, Callier, Ballèvre and Beaufrère19). If the extent of postprandial increase in circulating amino acids influences satiety, as was hypothesised by the amino static theory of Mellinkoff et al. (Reference Mellinkoff, Frankland, Boyle and Greipel20), consumption of different levels of casein-protein in a single meal should result in differences in subsequent satiety. We investigated possible differences in satiety ratings between a high- and a normal-casein-protein concentration and the mechanisms accompanying those differences. Casein was offered in a breakfast consisting of 20 % of the subject-specific daily energy requirements, with amounts of casein that represent the highest allowed protein intake per d, i.e. 25 % of energy (En%) from protein v. the lowest (normal) protein intake per d, 10 % of energy from protein(Reference Spaaij and Pijls21). Protein was exchanged with fat; carbohydrate content was kept constant at a level of 55 En% because of its effects on protein metabolism(Reference Munro22), resulting in a comparison of a high-protein–low-fat breakfast with a normal-protein–normal-fat breakfast with casein as the single protein type.
The aim of the present study was to compare the effects of a high v. a normal amount of casein-protein-containing breakfast on plasma amino acid concentrations, appetite profile, such as ratings of hunger, satiety, fullness, and desire to eat, plasma glucose, and possibly related plasma hormone levels of insulin, glucagon-like peptide 1 (GLP-1), and ghrelin and subsequent energy intake. In order to determine the moment in time that may be sensitive to show a possible difference in food intake we first assessed appetite profile ratings and ‘satiety’ hormones for 4 h and in the subsequent experiment energy intake was measured at the determined moment in time.
Subjects and methods
Subjects
Thirty healthy male and female volunteers (BMI 22–30 kg/m2; age 18–40 years) were recruited by advertisements in local newspapers and on notice boards at the university. They underwent a screening including medical history, measurement of body weight and height and cognitively restrained eating using a Dutch translation of the Three Factor Eating Questionnaire(Reference Stunkard and Messick23, Reference Westerterp-Plantenga, Westerterp, Rubbens, Verwegen, Richelet and Gardette24). Twenty-five subjects (eleven male, fourteen female) were selected on being in good health, non-smokers, non-vegetarian, not cognitively dietary restrained, not using medication apart from oral contraceptives and at most moderate alcohol users. Their mean age was 22 (sem 1) years, and their body weight was 74·4 (sem 1·8) kg (BMI 23·9 (sem 0·3) kg/m2). A written informed consent was obtained from these participants and the study protocol was approved by the Medical Ethical Committee of the Academic Hospital Maastricht.
Study design
A randomised, single-blind, within-subject experimental study was performed. All subjects came to the university on two occasions, separated by at least 1 week. On each test day subjects received a subject-specific standardised breakfast and appetite ratings and blood parameters were obtained for 4 h after breakfast.
After 2 months, when the sensitive moment in time was determined based on appetite profile ratings and concentrations of metabolites, subjects again came to the university on two occasions in a randomised, single-blind design, separated by at least 1 week. On each test day subjects received a subject-specific standardised breakfast and an ad libitum lunch was offered at the previously determined sensitive moment in time.
Breakfast
Breakfast was offered as a custard with casein (Calcium Caseinate S; DMV International, Veghel, The Netherlands) as the single protein source, with either protein, carbohydrate and fat at 10, 55 and 35 En% (normal protein) or protein, carbohydrate and fat at 25, 55 and 20 En% (high protein). The breakfast contained 20 % of daily energy requirements, calculated as BMR, according to the Harris–Benedict equations, multiplied by an activity index of 1·75(Reference Harris and Benedict25). The mean energy content of the breakfast was 2·52 (sem 0·07) MJ and the provided breakfasts were completely finished.
The custards were produced by NIZO Food Research b.v. (Ede, The Netherlands) and had tapioca starch (Farinex VA50T; AVEBE, Veendam, The Netherlands and Perfectamyl 3108; AVEBE, Veendam, The Netherlands) and sunflower-seed oil (Reddy; NV Vandemoortele, Roosendaal, The Netherlands) respectively as the carbohydrate and fat sources and were citrus–vanilla (Citrus, Vanilla; J.B. de Lange, Belfeld, The Netherlands) flavoured. Extensive product development and use of a taste panel led to custards that did not differ significantly in colour, taste or viscosity. The amino acid composition of the custards is presented in Table 1.
Amino acid content of the breakfasts given as a custard with either 10 % of energy from casein-protein or 25 % of energy from casein-protein (g amino acids/100 g custard)
* Glutamic acid = glutamine+glutamate.
† Aspartic acid = asparagine.
Lunch
Lunch consisted of Turkish bread (400 g) with egg salad (400 g) with 13, 41 and 46 En% protein, carbohydrate and fat with an energy density of 11·4 kJ/g. Subjects were instructed to eat till they were comfortably full.
Study protocol
The protocol started at 08.00 hours after an overnight fast from 22.00 hours. A Venflon catheter was placed in a superficial dorsal vein of the hand for blood sampling. To obtain arterialised venous blood samples the hand was placed in a thermostatically controlled hot box at 60°C for 20 min before the sampling time. A basal blood sample was taken and appetite ratings were scored. After 5 min, a second basal blood sample was obtained and breakfast was offered (t = 0 min) and completed within 20 min. After the first and the last bite, taste perception was scored. Appetite ratings were completed just before breakfast and at 20, 40, 60, 80, 100, 120, 180 and 240 min after breakfast. Blood samples for urea and amino acid determination were obtained at − 5 min and subsequently at the same time points as the appetite ratings; blood samples for the determination of glucose, insulin and ghrelin concentrations were obtained before and at 40, 60, 120 and 180 min after breakfast. Venous blood samples for the determination of GLP-1 concentration were obtained separately before, and at 30, 60, 90, 120 and 180 min after breakfast by means of a Venflon catheter placed in an antecubital vein(Reference Adam and Westerterp-Plantenga26). Subjects were allowed to drink maximally two glasses of water spread over the morning.
In the second set of experiments, the protocol started after an overnight fast from 22.00 hours to 08.30 hours with scoring appetite ratings. Breakfast was offered (t = 0 min) and completed within 20 min. Lunch was offered at the previously determined sensitive moment in time. Subjects were allowed to drink three glasses of water spread over the entire test period.
Measurements
Appetite profile
To determine the appetite profile, hunger, fullness, satiety, and desire to eat were rated on 100 mm visual analogue scales (VAS), anchored with ‘not at all’ and ‘extremely’ during the test day. VAS are often used to measure subjective appetite sensations and the validity and reproducibility have been shown in several studies(Reference Stubbs, Hughes, Johnstone, Rowley, Reid, Elia, Stratton, Delargy, King and Blundell27, Reference Flint, Raben, Blundell and Astrup28). Subjects were instructed to rate themselves by marking the scale at the point that was most appropriate to their feeling at that time. The distance from this point to the left end of the scale was measured in mm; changes from baseline (Δ) were calculated by subtracting the baseline score ( − 5 min) from the score at a certain time point.
Taste perception
Taste perception profiles of the custards were assessed after the first and the last bite of the breakfast using 100 mm VAS, anchored with ‘not at all’ and ‘extremely’ on the aspects of pleasantness, sweetness, sourness, saltiness, bitterness, savouriness, crispiness, and creaminess.
Energy intake
Lunch was weighed before and after eating and energy intake was calculated by multiplying the difference of the weight of the lunch by the energy value of the lunch as determined by the product labels (11·4 kJ/g).
Blood parameters
Blood was distributed into EDTA tubes for glucose, insulin and ghrelin measurement. For GLP-1 measurement blood was collected in EDTA tubes with added dipeptidyl peptidase IV inhibitor. For amino acid and urea determination, blood was collected in lithium heparin tubes. Blood samples were centrifuged at 4°C for 10 min at 3000 rpm. Hydrochloric acid and phenylmethylsulfonyl fluoride were added to plasma for active ghrelin determination. For amino acid analysis, 250 μl plasma was deproteinised by mixing it with 20 mg dry sulfosalicylic acid. For analysis of urea, 200 μl plasma was deproteinised by mixing it with 20 μl of a TCA solution (500 g/l). All samples were stored at − 80°C until further analysis.
Plasma glucose concentrations were determined using the hexokinase method (Glucose HK 125 kit; ABX Diagnostics, Montpellier, France). Insulin concentrations were measured by RIA (Linco Research Inc., St Charles, MO, USA). Plasma active ghrelin concentrations were measured by ELISA (Linco Research Inc.). Plasma active GLP-1 samples were analysed using ELISA (EGLP-35K; Linco Research Inc.).
Plasma concentrations of amino acids were determined with the use of a fully automated HPLC (Pharmacia, Woerden, The Netherlands), after precolumn derivatisation with o-phthaldialdehyde(Reference van Eijk, Rooyakkers and Deutz29). Plasma urea was analysed spectrophotometrically on a COBAS Mira S (Roche Diagnostica, Hoffman-La Roche, Basel, Switzerland).
Statistical analysis
Data are presented as mean changes from baseline with their standard errors, unless otherwise indicated(Reference Senn30). The area under the curve (AUC) of changes from baseline over time (4 h for appetite ratings, amino acid and urea concentrations; 3 h for glucose, insulin, GLP-1 and ghrelin concentrations) was calculated using the trapezoidal method. A repeated-measures ANOVA was carried out to determine possible differences between the high- and normal-protein breakfast. After the second set of experiments, a repeated-measures ANOVA was carried out to determine possible differences in energy intake between the breakfasts. A P value < 0·05 was regarded as statistically significant. Statistical procedures were performed using StatView 5.0 (SAS Institute Inc., USA, 1998).
Results
Appetite profile
Baseline ratings for appetite scores were not different among treatments (Table 2). The AUC of fullness ratings was increased after the breakfast with 25 % of energy from casein compared with the breakfast with 10 % of energy from casein (8522 (sem 872) v. 5459 (sem 974) mm VAS; P < 0·01; Fig. 1). Fullness ratings also were increased after a breakfast with 25 En% casein compared with a breakfast with 10 En% casein at several moments in time including at 180 and 240 min after breakfast (P < 0·01 and P < 0·01; Fig. 1). Satiety ratings were increased after the breakfast with 25 % of energy from casein compared with the breakfast with 10 % of energy from casein at 180 and 240 min (P < 0·05 and P < 0·05; Fig. 1).
(Mean values with their standard errors)
* Repeated-measures ANOVA.
Changes in satiety (a), fullness (b), hunger (c) and desire to eat (d) (all in mm visual analogue scale; VAS) after a casein-breakfast given as a custard with either 10 % of energy from casein-protein (△) or 25 % of energy from casein-protein (▲) expressed as change compared with baseline in twenty-five subjects (men and women). Values are means, with standard errors represented by vertical bars. *P < 0·05, **P < 0·01 (repeated-measures ANOVA).
Taste perception
Ratings of taste perception profiles and of pleasantness of taste of the custards were not different between the breakfast with 25 % of energy from casein and the breakfast with 10 % of energy from protein (Table 3).
(Mean values with their standard errors)
* Repeated-measures ANOVA; no significant differences.
Glucose
Baseline plasma glucose concentrations were not different among treatments (Table 2). The glucose response expressed as AUC was increased after the breakfast with 10 % of energy from casein (123·70 (sem 14·25) mmol/l × h) compared with the breakfast with 25 % of energy from casein (68·04 (sem 18·11) mmol/l × h; P < 0·05). Glucose concentration was increased after the breakfast with 10 % of energy from casein compared with the breakfast with 25 % of energy from casein at 40 and 60 min after breakfast (P < 0·05 and P < 0·05; Fig. 2).
Changes in glucose concentrations (mmol/l) after a casein-breakfast given as a custard with either 10 % of energy from casein-protein (△) or 25 % of energy from casein-protein (▲) expressed as change compared with baseline in twenty-five subjects (men and women). Values are means, with standard errors represented by vertical bars. *P < 0·05 (repeated-measures ANOVA).
Insulin
Baseline plasma insulin concentrations were not different among treatments (Table 2). Insulin concentration was increased after the breakfast with 10 % of energy from casein compared with the breakfast with 25 % of energy from casein at 40 min after breakfast (P < 0·05; Fig. 3).
Changes in insulin concentrations (mU/l) after a casein-breakfast given as a custard with either 10 % of energy from casein-protein (△) or 25 % of energy from casein-protein (▲) expressed as change compared with baseline in twenty-five subjects (men and women). Values are means, with standard errors represented by vertical bars. *P < 0·05 (repeated-measures ANOVA).
Glucagon-like peptide 1 and ghrelin
Baseline plasma GLP-1 and ghrelin concentrations were not different among treatments (Table 2). There were no significant differences in GLP-1 or ghrelin concentrations between a high- and a normal-casein breakfast (data not shown).
Amino acids and urea
Baseline plasma amino acid and urea concentrations were not different among treatments (Table 4). The AUC of the response of glutamate, asparagine, serine, glutamine, histidine, glycine, threonine, citrulline, arginine, alanine, taurine, α-aminobutyric acid, tyrosine, valine, methionine, isoleucine, phenylalanine, tryptophan, leucine, ornithine, lysine, branched-chain amino acids, total amino acids and urea are presented in Table 4; significant differences between treatments are indicated.
(Mean values with their standard errors)
*P < 0·05, **P < 0·01, ***P < 0·001.
† Repeated-measures ANOVA.
Compared with the breakfast with 10 % of energy from casein, almost all amino acids showed a prolonged elevation with a typical pattern after the breakfast with 25 % of energy from casein. Plasma amino acid concentrations rose immediately after breakfast to peak values at 40 min after breakfast. Then concentrations slightly decreased, with concentrations increasing again from 80 min onwards. The second peak levels were reached at 180 min after breakfast. To illustrate this phenomenon, Fig. 4 presents the plasma amino acid concentrations over time of the branched-chain amino acids and total amino acids. The prolonged elevated concentrations were shown with nearly all amino acids. Total amino acids and branched-chain amino acids as well as glutamate, asparagine, serine, glutamine, histidine, threonine, arginine, alanine, α-aminobutyric acid, tyrosine, valine, methionine, isoleucine, phenylalanine, tryptophan, leucine, ornithine and lysine concentrations were increased at 180 and 240 min after the breakfast with 25 % of energy from casein compared with the breakfast with 10 % of energy from casein (P < 0·05).
Changes in plasma branched-chain amino acid (BCAA) concentrations (μmol/l) (a) and plasma total amino acid (total AA) concentrations (μmol/l) (b) after a casein-breakfast given as a custard with either 10 % of energy from casein-protein (△) or 25 % of energy from casein-protein (▲) expressed as change compared with baseline in twenty-five subjects (men and women). Values are means, with standard errors represented by vertical bars. ***P < 0·001 (repeated-measures ANOVA).
The urea response expressed as AUC was increased after the breakfast with 25 % of energy from casein compared with a breakfast with 10 % of energy from casein (P < 0·001; Table 4).
Energy intake
Based on the results of appetite profile ratings and the concentrations of amino acids the ad libitum lunch was offered at 180 min after breakfast.
Energy intake at lunch was 3133 (sem 226) and 3080 (sem 229) kJ after the breakfasts with 10 % and 25 % of energy from protein, respectively (NS).
Discussion
Ratings of satiety and fullness were higher after a subject-specific breakfast consisting of 20 % of total daily energy requirements with casein at a concentration of 25 % of energy from protein compared with the breakfast with 10 % of energy from casein, particularly at 3 and 4 h after breakfast. Energy intake at lunch was not different after a high- or normal-casein breakfast. Sometimes it is suggested that protein-induced satiety is partly due to specific sensory effects(Reference Bertenshaw, Lluch and Yeomans8). There is, however, hardly any evidence for this suggestion, especially not in the case of amounts of protein of about 30 g in combination with carbohydrate and fat in a meal. Clearly, most amino acids evoke taste-aversive responses because they have a bitter or sour taste(Reference Kirimura, Shimizi, Kimizuka, Ninomiya and Katsuya31). This is why we did not use pure amino acids but applied complete proteins. Nevertheless, to avoid any specific sensory effect, food technology was involved to optimise taste and hedonic value of the breakfasts. The custards were vanilla–lemon flavoured and after being tested by a professional taste panel of NIZO Food Research taste perception and hedonic values again were evaluated by the subjects (see Table 2) and were excluded from affecting appetite profile ratings differently.
The increased satiety after the breakfast with 25 % of energy from casein compared with the breakfast with 10 % of energy from casein coincided with prolonged elevated concentrations of amino acids. Since postprandial amino acid profiles are likely to reflect rates of digestion, absorption and metabolism, the prolonged elevated concentrations indicate a difference in protein kinetics between the two breakfasts. Previously it has been shown that casein coagulates in the stomach which delays gastric emptying; therefore casein is considered as a ‘slow’ protein. The higher the casein concentration the slower the release of food into the duodenum(Reference Boirie, Dangin, Gachon, Vasson, Maubois and Beaufrère18, Reference Dangin, Boirie, Garcia-Rodenas, Gachon, Fauquant, Callier, Ballèvre and Beaufrère19, Reference Mahé, Roos, Benamouzig, Davin, Luengo, Gagnon, Gaussergès, Rautureau and Tomé32). This is reflected by the typical pattern of amino acid concentrations over time. The largest differences in amino acid concentrations between the breakfasts with 25 % and 10 % of energy from casein existed at 3 and 4 h after breakfast. These prolonged elevated concentrations may have contributed to the increased satiety ratings after the breakfast with 25 % of energy from casein, which is in line with Mellinkoff's amino static theory that states that a larger rise in plasma amino acids increases satiety(Reference Mellinkoff, Frankland, Boyle and Greipel20). The increased satiety ratings after the breakfast with 25 % of energy from casein may thus be explained by increased concentrations of amino acids caused by the delayed gastric emptying of casein.
Surprisingly, the insulin concentration was increased after the breakfast with 10 % of energy from casein whereas the glucose response expressed as AUC also was significantly increased after the breakfast with 10 % of energy from casein compared with the breakfast with 25 % of energy from casein whereas the carbohydrate content of the two breakfasts was exactly the same. The slower release of food into the duodenum after the high-casein breakfast also delayed and diminished the rise of glucose and subsequently insulin concentrations in the circulation. Previously, insulin concentrations have been shown not to increase after consumption of a meal with ‘slow’ proteins in healthy young adults(Reference Dangin, Boirie, Garcia-Rodenas, Gachon, Fauquant, Callier, Ballèvre and Beaufrère19). Protein kinetics, reflected by changes in plasma amino acid concentrations, were different between the high- and normal-casein breakfast. The high-casein breakfast revealed a plasma amino acid pattern that is typical for a ‘slow’ protein and that was, besides glucose and insulin responses, also reflected by the changes in GLP-1 and ghrelin concentrations. The absence of significant differences in GLP-1 or ghrelin concentrations between the high- and normal-casein breakfasts may be the result of the delayed gastric emptying and thus retarded entrance of food in the intestine followed by a diminished physiological response of GLP-1 secretion and a less pronounced decrease in ghrelin concentration. In summary, the breakfast with 25 % of energy from casein delayed gastric emptying more compared with the breakfast with 10 % of energy from casein resulting in less pronounced changes in insulin, GLP-1 and ghrelin.
In the literature differences in ‘satiety’ hormone responses between the different macronutrients have been shown(Reference Bowen, Noakes and Clifton12, Reference Bowen, Noakes, Trenerry and Clifton13, Reference Foster-Schubert, Overduin, Prudom, Liu, Callahan, Gaylinn, Thorner and Cummings33, Reference Erdmann, Lippl and Schusdziarra34). In a review by Cummings it is stated that protein intake does not affect ghrelin response particularly(Reference Cummings35). For instance, no differences in ghrelin concentrations after a high-protein (30 En% protein) compared with a normal-protein diet (10 En% protein) were observed, when the high- or normal-protein diet was given during three meals over 1 d(Reference Lejeune, Westerterp, Adam, Luscombe-Marsh and Westerterp-Plantenga36). Foster-Schubert, however, reported a stronger suppression of ghrelin by proteins compared with fat or carbohydrates(Reference Foster-Schubert, Overduin, Prudom, Liu, Callahan, Gaylinn, Thorner and Cummings33), with a test meal extremely high in protein with hardly any of the other macronutrients present. This makes comparisons with less extreme meals, such as in the present study a moderately high-protein meal that is representative for a relatively high daily protein intake of 25 % of energy from protein with a normal amount of carbohydrates (55 En%) and a low amount of fat (20 En%), difficult. Other observations showed that different types of protein modified ‘satiety’ hormone responses differently in some(Reference Hall, Millward, Long and Morgan11, Reference Lang, Bellisle, Alamowitch, Craplet, Bornet, Slama and Guy-Grand15) but not all studies(Reference Lang, Bellisle, Oppert, Craplet, Bornet, Slama and Guy-Grand14, Reference Uhe, Collier and O'Dea16). In the present study there were no differences in GLP-1 and ghrelin between two levels of casein-protein, probably due to the fact that casein is a ‘slow’ protein. This would result in a compensation of the effect of concentration by pace of nutrient-stimulated hormone release.
Despite significantly increased ratings of satiety after the breakfast with 25 % of energy from casein at 3 h after breakfast, energy intake was similar after the high- and normal-casein breakfast. Apparently the difference in satiety ratings of 12–15 mm VAS was not large enough to induce a significant effect on food intake. Previously, Diepvens et al. also reported a significant suppressive effect of a preload on appetite ratings whereas there was no effect on ad libitum energy intake 4 h after the preload. They concluded that the difference in hunger scores may be too small to exert an effect on subsequent energy intake and that timing is of major importance to observe significant effects on food intake (K Diepvens, J Steijns, P Zuurendonk and MS Westerterp-Plantenga, unpublished results). In the past there have been experiments that showed differences in subsequent energy intake between types of protein offered as a preload without significant differences in appetite ratings(Reference Hall, Millward, Long and Morgan11, Reference Anderson, Tecimer, Shah and Zafar37, Reference Borzoei, Neovius, Barkeling, Teixeira-Pinto and Rossner38). In case subsequent energy intake is affected without pre-prandial indications of appetite profile ratings, it may well be that the combination of the digested food from the previous preload or meal with the new digested food in the gut may evoke uncomfortable feelings that stop further energy intake. Furthermore, differences in timing may explain different results; timing is essential in studying ad libitum energy intake after a preload or a meal as shown by Anderson et al. (Reference Anderson, Tecimer, Shah and Zafar37). In accordance with other studies (K Diepvens, J Steijns, P Zuurendonk and MS Westerterp-Plantenga, unpublished results), the present study shows that differences in appetite ratings thus need to be at least larger than 15 mm VAS in order to have a significant effect on subsequent energy intake. Although the high-casein breakfast was rated as more satiating than the normal-casein breakfast the difference was not large enough to induce a reduction in energy intake.
Urea concentrations were elevated more after the high-casein breakfast compared with the normal-protein breakfast with casein. The high urea concentrations reflect an excess of amino acids and a state of positive protein balance after the high-protein breakfast. Postprandial protein synthesis has high ATP costs(Reference van Milgen39) and when amino acids are given in excess of protein deposition, amino acid oxidation plays a major role in energy expenditure and protein oxidation(Reference Pannemans, Wagenmakers, Westerterp, Schaafsma and Halliday40, Reference Robinson, Jaccard, Persaud, Jackson, Jequier and Schutz41) that previously has been shown to be related to diet-induced thermogenesis and increased satiety(Reference Crovetti, Porrini, Santangelo and Testolin9, Reference Westerterp-Plantenga, Rolland, Wilson and Westerterp42).
This is the first study that investigated acute differences in satiety between two concentrations of casein; previously the satiating properties of casein only have been compared with other protein types(Reference Hall, Millward, Long and Morgan11, Reference Lang, Bellisle, Oppert, Craplet, Bornet, Slama and Guy-Grand14). The present study shows that a breakfast with 25 % of energy from casein is rated as being more satiating than a breakfast with 10 % of energy from casein at 3 and 4 h after breakfast, coinciding with prolonged elevated concentrations of plasma amino acids, but does not reduce subsequent energy intake.
Acknowledgements
M. A. B. V., A. G. N., A. H.-W., K. R. W., M. P. K. J. E., R. J. M. B., N. E. P. D. and M. S. W.-P. designed the study. M. A. B. V. and A. H.-W. collected and analysed the data. M. A. B. V. wrote the manuscript and A. G. N., K. R. W., M. P. K. J. E., N. E. P. D. and M. S. W.-P. contributed to interpretation of the data and reviewed the manuscript. The study was executed under supervision of A. G. N., K. R. W. and M. S. W.-P. None of the authors had a personal or financial conflict of interest.
