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Comparison of meat versus soya based high-protein diets on faecal microbiota and microbial metabolites

Published online by Cambridge University Press:  19 October 2020

S.W. Gratz ,
L. Scobbie ,
A.J. Richardson ,
X. Zhang ,
C. Fyfe ,
F.M. Farquharson ,
G. Duncan ,
J. Filipe ,
W.Y. Zhu  and
A.M. Johnstone
...Show all authors
S.W. Gratz
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
L. Scobbie
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
A.J. Richardson
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
X. Zhang
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China
C. Fyfe
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
F.M. Farquharson
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
G. Duncan
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
J. Filipe
Affiliation:
Biomathematics and Statistics Scotland, Aberdeen, UK
W.Y. Zhu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China
A.M. Johnstone
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
P. Louis
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
W.R. Russell
Affiliation:
Rowett Institute, University of Aberdeen, Aberdeen, UK
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Abstract

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High-protein, low carbohydrate diets are popular and efficient weight loss regimes, but we have previously shown that meat based high-protein diets had a negative effect on faecal metabolites and gut microbiota (Reference Russell, Gratz, Duncan and Holtrop1). We therefore conducted a study to investigate whether vegetarian high-protein diets would have the same effect.

After 3 days on a balanced weight maintenance diet, 20 obese men received two high-protein weight loss diets with either beef or soya as the main protein source for 2 weeks each (Reference Neacsu, Fyfe, Horgan and Johnstone2). Faecal samples were collected at the end of each dietary period and faecal microbial DNA was extracted for qPCR bacterial profiling. Faecal water was extracted by high-speed centrifugation for short chain fatty acid (SCFA) analysis by GC and metabolite analysis by LC-MS/MS. Data were analysed by ANOVA with volunteer as a random effect and diet as a fixed effect and where diet effect was significant (P < 0.05), means were compared with post hoc t-test.

The soya diet resulted in significant (p < 0.05) shifts in gut microbiota composition increasing the proportions of Faecalibacterium prausnitzii and Lactobacillus spp. while decreasing the proportions of bacteria related to some Ruminococcus spp., Oscillibacter, Desulfovibrio spp. and Methanogens compared to the meat diet. Total faecal SCFA and the proportions of individual SCFA did not differ between individuals consuming meat or soya diets (p > 0.05). Protein-derived metabolites (phenylacetic acid, phenylpyruvic acid and the proportion of branched-chain fatty acids iso-butyrate and iso-valerate) tended to be higher on both high-protein diets with no significant differences between soya and meat diets (p > 0.05).

Several toxic and proliferative polyamines were significantly lower (p < 0.05) on the soya diet compared to meat (cadaverine 69.4% decrease, piperidine 66.0% decrease, spermidine 49.4% decrease, spermine 81.5% decrease).

Heterocyclic amines were also significantly (p < 0.05) lower on the soya diet compared to meat (IQ 26.7% decrease, MeIQ 36.7% decrease, MeIQx 44.8% decrease, 4,8DiMeIQx 69.6% decrease, DMIP 30.9% decrease). N-nitrosocompounds were also significantly lower on soya diet compared to meat (41.1% decrease) whereas genotoxicity of faecal waters remained unaltered by diet changes (p = 0.56).

These results suggest that high-protein diets based on plant protein are less problematic in terms of intestinal health when compared to meat-based diets and might offer a safe option to support weight loss regimes.

References

Russell, WR, Gratz, SW, Duncan, S, Holtrop, G, et al. (2011) Am J Clin Nutr 93, 1062–1072.Google Scholar
Neacsu, M, Fyfe, C, Horgan, G & Johnstone, AM (2014) Am J Clin Nutr 100, 548–558.Google Scholar