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Study into the polyphenol content and antioxidant activity of rapeseed pomace extracts

Published online by Cambridge University Press:  20 June 2016

F. Pohl ,
M. Goua ,
G. Bermano ,
W. R. Russell ,
P. Maciel  and
P. Kong Thoo Lin
F. Pohl
Affiliation:
School of Pharmacy and Life Science, Robert Gordon University, Aberdeen, AB10 1GJ
M. Goua
Affiliation:
School of Pharmacy and Life Science, Robert Gordon University, Aberdeen, AB10 1GJ
G. Bermano
Affiliation:
School of Pharmacy and Life Science, Robert Gordon University, Aberdeen, AB10 1GJ
W. R. Russell
Affiliation:
Rowett Institute of Nutrition and Health, University of Aberdeen, AB21 9SB
P. Maciel
Affiliation:
Life and Health Science Institute (ICVS), Universidade do Minho, Braga, Portugal
P. Kong Thoo Lin
Affiliation:
School of Pharmacy and Life Science, Robert Gordon University, Aberdeen, AB10 1GJ
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Abstract

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This abstract was awarded the student prize.

Rapeseed pomace (RSP) is a waste product obtained after edible oil production from Brassica napus Reference Szydłowska-Czerniak 1 . This study aimed to determine the polyphenol content, radical scavenging activity, ferric iron reducing antioxidant power and the oxygen-radical absorbance capacity assay, of RSP extracts, with regard to their potential application in the treatment or prevention of neurodegenerative diseasesReference Kennedy and Wightman 2 . High performance liquid chromatography/mass spectrometry was applied to determine ubiquitous polyphenolic compoundsReference Russell 3 .

Three different extraction methods (Soxhlet-, ultra sonic assisted- and accelerated solvent extractions) were applied on 2 harvest years (2012, 2014). From the extracts obtained, the total phenolic content (Folin–Ciocalteu assay) was found to be between 5·54 (SD 0·28) and 2·48 (SD 0·06) gallic acid equivalents/100 mg dry weight. The ferric iron reducing antioxidant power (FRAP assay) is ranging from 0·834 (SD 0·01) to 0·34 (SD 0·01) Trolox equivalents/100μg. The IC50 values for the radical scavenging (DPPH) assay were found to be between 49·23 (SD 14·00) and 180·30 (SD 16·16) μg/mL. Liquid chromatography-mass spectrometry of the RSP extracts showed substantial presence of several phenolic compounds, the most abundant one being sinapic acid (7496·7 (SD198·9) − 1923·3 (SD18·4) mg/kg). Significant differences (2-way ANOVA, p < 0·0001) were found between the 3 different extraction techniques for all the tested antioxidant properties. The strong antioxidant properties demonstrated by the RSP extracts in this study warrants further investigation for their potential use in the treatment or prevention of oxidative stress related diseases.

This work was supported financially by the Moulton Barrett Scholarship from TENOVUS Scotland and Macintosh of Glendaveny by providing the rapeseed pomace.

References

1. Szydłowska-Czerniak, A. Rapeseed and its products–sources of bioactive compounds: a review of their characteristics and analysis. Crit. Rev. Food Sci. Nutr. 53, 307–30 (2013).CrossRefGoogle ScholarPubMed
2. Kennedy, D. O. & Wightman, E. L. Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain function. Adv. Nutr. 2, 32–50 (2011).CrossRefGoogle ScholarPubMed
3. Russell, W. R. et al. High-protein, reduced-carbohydrate weight-loss diets promote metabolite profiles likely to be detrimental to colonic health. Am. J. Clin. Nutr. 93, 1062–1072 (2011).CrossRefGoogle ScholarPubMed