Hostname: page-component-89b8bd64d-5bvrz Total loading time: 0 Render date: 2026-05-07T07:53:08.168Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular cloning of a putative agglutination/immobilization antigen located on the surface of a novel agglutination/immobilization serotype of Cryptocaryon irritans

Published online by Cambridge University Press:  13 June 2008

A. HATANAKA ,
N. UMEDA  and
N. HIRAZAWA
A. HATANAKA*
Affiliation:
Central Research Laboratories of Nippon Suisan Kaisha Ltd, 559-6 Kitano-Machi, Hachioji, Tokyo 192-0906, Japan
N. UMEDA
Affiliation:
Marine Biological Technology Center of Nippon Suisan Kaisha Ltd, 508-8 Ariakeura Tsurumi, Saiki-Shi, Oita 876-1204, Japan
N. HIRAZAWA
Affiliation:
Central Research Laboratories of Nippon Suisan Kaisha Ltd, 559-6 Kitano-Machi, Hachioji, Tokyo 192-0906, Japan
*
*Corresponding author: Central Research Laboratories of Nippon Suisan Kaisha Ltd, 559-6 Kitano-Machi, Hachioji, Tokyo 192-0906, Japan. Tel: +81 426 56 5195. Fax: +81 426 56 5188. E-mail: hatanaka@nissui.co.jp
Get access Rights & Permissions [Opens in a new window]

Summary

A surface agglutination/immobilization antigen was purified from the novel agglutination/immobilization serotype (serotype G37) of the ciliated protozoan Cryptocaryon irritans, a parasite of seawater fishes. Serum from fish immunized with C. irritans theronts had agglutination/immobilization activity against theronts in vitro. However, fish and rabbit antisera raised against serotype G32 (reported previously) caused little agglutination/immobilization of serotype G37 theronts. Immunological analysis indicated that the 37 kDa theront surface membrane protein may be the agglutination/immobilization antigen of this serotype. The full-length 37 kDa antigen cDNA contained 1171 base pairs, encoding a 331–amino acid protein with hydrophobic N- and C-termini, which are characteristically found in proteins containing a C-terminal glycosylphosphatidylinositol anchor. In addition, the genetically characterized nucleotide sequences of the first internal transcribed spacer region of ribosomal DNA of these 2 serotypes were compared. The internal transcribed spacer rDNA sequence of serotype G32 was identical to that of isolates from Pingtung, Taiwan, and from the USA. On the other hand, the sequences of serotype G37 were not identical to those of any C. irritans isolate.

Keywords

Cryptocaryon irritanswhite spot diseasevaccinationagglutination/immobilization antigenserotyperibosomal DNA (rDNA)

Information

Type
Original Articles
Information
Parasitology , Volume 135 , Issue 9 , August 2008 , pp. 1043 - 1052
Copyright
Copyright © 2008 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

Azzouz, N., Ranck, J. L. and Capdeville, Y. (1990). Purification of the temperature-specific surface antigen of Paramecium primaurelia with its glycosyl-phosphatidylinositol membrane anchor. Protein Expression and Purification 1, 13–18.Google Scholar
Caron, F. and Meyer, E. (1989). Molecular basis of surface antigen variation in paramecia. Annual review of Microbiology 43, 23–42.CrossRefGoogle ScholarPubMed
Clark, T. G., Dickerson, H. W. and Findly, R. C. (1988). Immune response of channel catfish to ciliary antigens of Ichthyophthirius multifiliis. Developmental and Comparative Immunology 12, 581–594.CrossRefGoogle ScholarPubMed
Colorni, A. and Burgess, P. J. (1997). Cryptocaryon irritans Brown 1951, the cause of white spot disease in marine fish: an update. Aquarium Sciences and Conservation 1, 217–238.CrossRefGoogle Scholar
Dickerson, H. W. and Clark, T. G. (1996). Immune response of fishes to ciliates. Annual Review of Fish Disease 6, 107–120.CrossRefGoogle Scholar
Dickerson, H. W., Clark, T. G. and Findly, R. C. (1993). Serotype variation among isolates of Ichthyophthirius multifiliis based on immobilization. Journal of Eukaryotic Microbiology 40, 816–820.CrossRefGoogle ScholarPubMed
Diggles, B. K. and Adlard, R. D. (1997). Intraspecific variation in Cryptocaryon irritans. The Journal of Eukaryotic Microbiology 44, 25–32.CrossRefGoogle ScholarPubMed
Eisenhaber, B., Bork, P. and Eisenhaber, F. (1999). Prediction of potential GPI-modification sites in proprotein sequences. Journal of Molecular Biology 292, 741–758.CrossRefGoogle ScholarPubMed
Gerber, L. D., Kodukula, K. and Udenfriend, S. (1992). Phosphatidylinositol glycan (PI-G) anchored membrane proteins. Amino acid requirements adjacent to the site of cleavage and PI-G attachment in the COOH-terminal signal peptide. The Journal of Biological Chemistry 267, 12168–12173.Google Scholar
Hatanaka,A. ,A., Umeda, N., Yamashita, S. and Hirazawa, N. (2007). Identification and characterization of a putative agglutination/immobilization antigen on the surface of Cryptocaryon irritans. Parasitology 134, 1163–1174.Google Scholar
Hirazawa, N., Oshima, S., Hara, T., Mitsuboshi, T. and Hata, K. (2001). Antiparasitic effect of medium-chain fatty acids against the ciliate Cryptocaryon irritans infestation in the red sea bream Pagrus major. Aquaculture 198, 219–228.Google Scholar
Ko, Y. G. and Thompson, G. A. Jr. (1992). Immobilization antigens from Tetrahymena thermophila are glycosyl-phosphatidylinositol-linked proteins. The Journal of Protozoology 39, 719–723.Google Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, Lomdon 227, 680.CrossRefGoogle ScholarPubMed
Smith, D. L., Berkowitz, M. S., Potoczak, D., Krause, M., Raab, C., Quinn, F. and Doerder, F. P. (1992). Characterization of the T, L, I, S, M and P cell surface (immobilization) antigens of Tetrahymena thermophila: molecular weights, molecular isoforms, and crossreactivity of antisera. The Journal of Protozoology 39, 420–428.CrossRefGoogle Scholar
Sun, H. Y., Zhu, X. Q., Xie, M. Q., Wu, X. Y., Li, A. X., Lin, R. Q. and Song, H. Q. (2006). Characterization of Cryptocaryon irritans isolates from marine fishes in Mainland China by ITS ribosomal DNA sequences. Parasitology Research 99, 160–166.Google Scholar
Swennes, A. G., Findly, R. C. and Dickerson, H. W. (2007). Cross-immunity and antibody responses to different immobilization serotypes of Ichthyophthirius multifiliis. Fish Shellfish Immunology 22, 589–597.Google Scholar
Wang, X., Clark, T. G., Noe, J. and Dickerson, H. W. (2002). Immunisation of channel catfish, Ictalurus punctatus, with Ichthyophthirius multifiliis immobilisation antigens elicits serotype-specific protection. Fish Shellfish Immunology 13, 337–350.CrossRefGoogle ScholarPubMed
Yambot, A. V., Song, Y. L. and Sung, H. H. (2003). Characterization of Cryptocaryon irritans, a parasite isolated from marine fishes in Taiwan. Diseases of Aquatic Organisms 54, 147–156.Google Scholar
Yoshinaga, T. and Dickerson, H. W. (1994). Laboratory propagation of Cryptocaryon irritans Brown, 1951 on salt water-adapted black mollies (Poecilia latipinna). Journal of Aquatic Animal Health 6, 197–201.2.3.CO;2>CrossRefGoogle Scholar