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Effect of exercise and high carbohydrates diet in the T-lymphocytes of Peyer's patches of Balb/c mice

Published online by Cambridge University Press:  04 August 2011

B. E. Martínez-Carrillo ,
M. G. Zúñiga-Torres ,
R. A. Jarillo-Luna ,
R. Valdés-Ramos ,
V. Rivera-Aguilar ,
R. Campos-Rodriguez ,
R. V. Pardo-Morales  and
C. Rosales-Gómez
B. E. Martínez-Carrillo
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México, México Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, México
M. G. Zúñiga-Torres
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México, México
R. A. Jarillo-Luna
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, México
R. Valdés-Ramos
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México, México
V. Rivera-Aguilar
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, México
R. Campos-Rodriguez
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, México
R. V. Pardo-Morales
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México, México
C. Rosales-Gómez
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, México
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Abstract

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It has been shown that exercise induces considerable changes in the body, particularly in the mucosal immune system(Reference Pedersen and Hoffman-Goetz1); the current lifestyles have promoted an increase in the consumption of high carbohydrate diets (↑CHO); these two factors result in particular effects on Peyer's patches (PP)(Reference Rogers, Berrigan and Zaharoff2). The objective of this study was to evaluate the effect of moderate exercise and ↑CHO on PP's T-cells (TLc) in Balb/c young mice. Thirty-two 21-d-old male mice were divided into four groups, two groups were taught to swim for 30 min 5 d per week during 9 weeks; one of which was fed a commercial control diet (CONTROL/E) and the other was fed a high carbohydrate diet (↑CHO/E) (DIO Rodent Purified Diet, Cat. 58Y2; energy: 4.65 kcal/g (19.4556 kJ/g), the other two groups were fed the same diets but were not made to swim (CONTROL and ↑CHO). Small intestines were dissected, PP were cut, macerated, filtered and centrifuged to obtain the lymphocyte pellet; cells were stained with anti-CD3+, CD4+ and CD8+ antibodies and analysed by flow cytometry (Facs Diva, BD®). The experimental diet increased the weight of mice who exercised (23.5, sd 1.3) compared with the non-exercise group (19.4, sd 1.8) and the control group (20.2, sd 1.4), P⩽0.005. CD3+ TLc were decreased in the ↑CHO/E group (31.1, sd 1.0) compared with the CONTROL/E group (38.8, sd 2.5), T 8.103, P⩽0.001; CD3+/CD4+ (23.2, sd 0.2), CD3+/CD8+ (3.5±0.5) also decreased compared with CONTROL/E group (33.5, sd 1.97 and 4.9, sd 0.5; t 14.8 and t 6.014 respectively, P⩽0.001). The combination of exercise and ↑CHO decreased significantly PP TLc, we can conclude that the intake of a high carbohydrate diet together with exercise attenuates lymphocytes transit, as well as increasing the weight of the mice (Fig. 1).

Fig. 1.

Percentage of lymphocytes CD3+, CD3+/CD4+ and CD3+/CD8+ from PP from Balb/c mice, feeding with standard diet (CONTROL) or high carbohydrate diet (↑CHO); with or without 30 min of exercise (/E). Values are show in means and standard deviations (sd) n 8, differences were statistically significant by Student's t test at P⩽0.001. CONTROL: group with standard diet without exercise; ↑CHO: group with high carbohydrate diet; CONTROL/E: group with standard diet with exercise; ↑CHO/E: group feeding with high carbohydrate diet, and with exercise.

This project was financed by Universidad Autónoma del Estado de México.

References

1.Pedersen, BK & Hoffman-Goetz, L (2000) Physiol Rev 80, 1055–1081.CrossRefGoogle Scholar
2.Rogers, CJ, Berrigan, D, Zaharoff, DA et al. (2008) J Nutr 138, 115–122.CrossRefGoogle Scholar
Figure 0

Fig. 1. Percentage of lymphocytes CD3+, CD3+/CD4+ and CD3+/CD8+ from PP from Balb/c mice, feeding with standard diet (CONTROL) or high carbohydrate diet (↑CHO); with or without 30 min of exercise (/E). Values are show in means and standard deviations (sd) n 8, differences were statistically significant by Student's t test at P⩽0.001. CONTROL: group with standard diet without exercise; ↑CHO: group with high carbohydrate diet; CONTROL/E: group with standard diet with exercise; ↑CHO/E: group feeding with high carbohydrate diet, and with exercise.