Introduction
Among the next tools to search for signs of past or present life in our Solar System, several instruments based on the biochip technology have been proposed in the framework of planetary exploration. A biochip is a miniaturized device composed of molecular recognition tools (or affinity receptors) like antibodies (Parro et al. Reference Parro2005, Reference Parro2011a; Sims et al. Reference Sims, Cullen, Bannister, Grant, Henry, Jones, McKnight, Thompson and Wilson2005, Reference Sims2012; Baqué et al. Reference Baqué, Le Postollec, Coussot, Moreau, Desvignes, Incerti, Moretto, Dobrijevic and Vandenabeele-Trambouze2011b; de Diego-Castilla et al. Reference de Diego-Castilla, Cruz-Gil, Mateo-Martí, Fernández-Calvo, Rivas and Parro2011) or aptamers (Baqué et al. Reference Baqué2011a), which allows the detection of hundreds of different compounds in a single assay. Widely developed for biotechnology use and medical or environmental diagnostics (see e.g. Wang Reference Wang2006), miniaturized instruments based on biochips have been indeed proposed and studied for biosignature detection in an astrobiological context since more than 15 years (McKay et al. Reference McKay, Steele, Allen, Thomas-Keptra, Schweitzer, Priscu, Sears, Avci and Firman2000; Parro et al. Reference Parro2005; Sims et al. Reference Sims, Cullen, Bannister, Grant, Henry, Jones, McKnight, Thompson and Wilson2005; Le Postollec et al. Reference Le Postollec2007).
Mars, one of the most probable planetary body where to find signs of extinct or extant life outside of Earth, is the target of many upcoming dedicated missions: ESA-Roscosmos’ ExoMars rover in 2016–2018, the National Aeronautics and Space Administration (NASA)'s Mars2020 rover (a follow-up of the Curiosity rover) and the Icebreaker mission concept proposed for a 2021 launch to be part of NASA's Discovery program (McKay et al. Reference McKay2013). Different space instruments based on the biochip technology and using antibodies have been proposed for these future missions: the Life Marker Chip (LMC) (Martins Reference Martins2011; Sims et al. Reference Sims2012; Sephton et al. Reference Sephton, Sims, Court, Luong and Cullen2013), and the Signs Of Life Detector (SOLID) (Parro et al. Reference Parro2005, Reference Parro2008, Reference Parro2011a, Reference Parrob). Another project, the Biochip for Organic Matter Analysis in Space (BiOMAS), proposes to combine both antibodies and aptamers in a single instrument (Le Postollec et al. Reference Le Postollec2007; Baqué et al. Reference Baqué2011a, Reference Baqué, Le Postollec, Coussot, Moreau, Desvignes, Incerti, Moretto, Dobrijevic and Vandenabeele-Trambouzeb). Recently, first in the framework of Mars2020 announcement of opportunity, and then in the framework of NASA's Discovery 2014 announcement of opportunity, these different teams have united to work on the SOLID instrument proposal for the Icebreaker mission and thus to contribute with their different expertise to improve the technological readiness level of a biochip-based instrument for space exploration (McKay et al. Reference McKay2013; Smith & Parro Reference Smith and Parro2014; Manchado et al. Reference Manchado, Sebastián, Romeral, Sobrado-Vallecillo, Herrero, Compostizo, Gómez-Elvira and Parro2015).
Indeed, although biochips are known to be very sensitive tools to detect specific target molecules, their sensitivity is related to the presence of functional affinity receptors. In order to develop a ‘space biochip’, it is thus necessary to ensure that these biological receptors will survive space hazards. In particular, due to the very sparse data on this topic, it is important to determine the behaviour of these biological receptors under cosmic particles irradiation.
Le Postollec et al. (Reference Le Postollec2009a) performed simulations with the Geant4 Monte Carlo toolkit in order to estimate the radiation environment that a biochip would face if it were placed into a rover dedicated to explore Mars’ surface. Ionizing doses accumulated and fluxes of particles entering the biochip have been established for both the Earth-Mars transit and the journey on Mars’ surface. Neutrons and gammas appear as dominant radiation species on Mars’ soil whereas protons dominate during the interplanetary travel. These results have been confirmed by other studies done by McKenna-Lawlor et al. (Reference McKenna-Lawlor, Gonçalves, Keating, Reitz and Matthiä2012) and Derveni et al. (Reference Derveni, Hands, Allen, Sims and Cullen2012). Moreover, these simulations can today be confronted to the real radiation environment of an actual mission to Mars as it was monitored by the Radiation Assessment Detector instrument on-board the Mars science laboratory spacecraft on cruise to Mars and continue to be recorded by the rover Curiosity directly on its surface (Hassler et al. Reference Hassler2013; Zeitlin et al. Reference Zeitlin2013; Kim et al. Reference Kim2014). Indeed, the total cosmic radiation dose rate of 210 ± 40 µGy day−1 (Hassler et al. Reference Hassler2013) recorded at Gale Crater by Curiosity and the one measured inside the Mars Science Laboratory spacecraft during its cruise to Mars (481 ± 80 µGy day−1) (Zeitlin et al. Reference Zeitlin2013) proved to be in the same order of magnitude as model predictions with respectively ~840 µGy day−1 (without any shielding) for the Martian surface and ~240 µGy day−1 for the Earth–Mars transit considering only galactic cosmic rays contribution (Le Postollec et al. Reference Le Postollec2009a).
Considering the lack of experimental data about cosmic rays effect on antibodies, particularly under lyophilized (freeze-dried) state, our team decided to investigate the effects of different types of particles at several energies. Our objective is first to study and measure cosmic rays effects on biological receptors and second to define well-adapted protections for a biochip-based instrument if we find evidences that cosmic rays might have deleterious effect on their performances. In the first study, Le Postollec et al. (Reference Le Postollec, Coussot, Baqué, Incerti, Desvignes, Moretto, Dobrijevic and Vandenabeele-Trambouze2009b) performed neutrons irradiation on both antibodies and fluorescein dyes (used for detection of recognition events) at two energies (0.6 and 6 MeV) and with different fluences. Sample analyses demonstrated that, in tested conditions, neutrons do not affect antibody recognition capability and fluorescence dye intensity. More recently, the effects of 2 MeV protons on antibody performances (Baqué et al. Reference Baqué, Le Postollec, Coussot, Moreau, Desvignes, Incerti, Moretto, Dobrijevic and Vandenabeele-Trambouze2011b) were investigated. These studies showed that this irradiation process did not affect the performances of antibodies as molecular recognition tools. In addition, printed antibody and Alexa-647 fluorescent dye were demonstrated to be stable between 1.18 and 1.33 MeV gamma radiation (de Diego-Castilla et al. Reference de Diego-Castilla, Cruz-Gil, Mateo-Martí, Fernández-Calvo, Rivas and Parro2011). Finally, Derveni et al. (Reference Derveni, Hands, Allen, Sims and Cullen2012) tested five antibodies freeze-dried in a variety of protective molecular matrices and exposed to 50 MeV protons. They showed that at a representative Mars-mission-dose, none of the antibodies studied exhibited any evidence of activity loss due to the radiation.
In the present paper, we broaden these previous studies to test the effect of electrons, carbon ions, protons (at different energies) and neutrons (at higher energies) on the recognition capability of antibodies (summarized in Fig. 1). As protons and neutrons dominate the radiation environment during the Earth–Mars transit and on the Martian surface, we tested different high-energy particles from 15 to 50 MeV at different fluences. Moreover, other damaging particles are significantly present in cosmic and solar radiations such as carbon ions and electrons.
Simulated spectra of particle fluxes, as a function of energy, during the Earth–Mars transit (left) and at Mars’ surface (right) with the energy range of particles investigated in this study (red zone). This figure is an adaptation of Fig. 8 in Le Postollec et al. (Reference Le Postollec2009a).
Chemicals and biological materials used to perform the experiments are given in the second section ‘Material’. The third section ‘Method’ describes samples preparation, particles irradiation parameters and analysis protocols. ‘Results’ are presented in the fourth section. The last section ‘Discussion/Conclusion’ draws conclusions on this study.
Material
Monoclonal anti-Horseradish Peroxydase (HRP) antibodies were obtained from Antibodies-online (Germany), HRP (type II), O-Phenylenediamine dihydrochloride (OPD), NaH2PO4, Tween® 20, sodium acetate, sucrose, sodium azide, NaOH, H2O2, BSA, (L)-Histidine and (D)-Arginine L-tyrosinamide, fluorescein and Tris(hydroxymethyl) aminomethane were purchased from Sigma Aldrich (Saint-Quentin, France). NaCl and MgCl2 were obtained from Chimie-Plus laboratoires (Bruyères de Pouilly, France) and Panreac Quimica (Barcelona, Spain), respectively.
Chemicals were analytical grade and were used as received. DNA-Bind™ plates were obtained from Corning (Netherlands) and Maxisorp™ plates were obtained from VWR (France). Optical density of the reaction products was measured on a Tecan Infinite M200 microplate reader (Lyon, France) at 492 nm.
Method
Sample preparation
Our Biochip models are small polymer containers, called micro-wells, where antibodies samples are placed for the experiments. DNA-Bind™ plates were used for covalent grafting (N-Oxysuccinimide functionalization allows random amine binding, Moreau et al. Reference Moreau, Faye, Baqué, Desvignes, Coussot, Pascal and Vandenabeele-Trambouze2011) whereas Maxisorp™ ones were used as sample containers for free antibodies. The samples preparation was done following the same protocol as in Baqué et al. (Reference Baqué2011a).
Briefly, antibodies were irradiated under two different states: grafted and free. All samples were freeze-dried using the freeze-drying buffer described in Baqué et al. (Reference Baqué2011a, Reference Baqué, Le Postollec, Coussot, Moreau, Desvignes, Incerti, Moretto, Dobrijevic and Vandenabeele-Trambouzeb) and then sealed in a FoodSaver™ bag in dry atmosphere (silica gel was added in the bag) and stored in the dark at 4 °C before irradiation. All irradiation effects were estimated on freeze-dried samples.
Irradiation parameters
Conditioning of samples during irradiation
In order to prevent potential degradations due to environmental changes (contact with air, moisture, potential organic contaminants, etc.), all samples were irradiated under their protecting packaging.
Micro-wells were irradiated directly in their sealed bags. The effect of the sealed bag, considering its thickness and composition, was assessed using simulations performed with the Geant4 toolkit (Agostinelli et al. Reference Agostinelli2003; Allison et al. Reference Allison2006). We determined that the influence of the bag during irradiations was negligible as very few particles were stopped by this additional plastic layer and very few secondary particles were created (data not shown).
Methodology adopted to choose irradiation parameters
Numerical simulations give a basis to select the types of particles, energies and fluences that we have to consider for irradiation experiments. However, this choice mainly depends on technical constraints and the availability of irradiation facilities. As an example, it is generally not possible to conduct ground-based experiments with the very low flux of particles and the long duration of irradiation (months or years) encountered in interplanetary space. In addition, due to analysis constraints (limit of detection, uncertainties), it is also necessary to choose adequate irradiation parameters to ensure that potential effects of particles on our targets will be measurable.
In the present study, when possible, we have chosen to use fluences in the same order of magnitude as the surface density of grafted antibodies. The objective of our experiments was to study the interaction between different types of particles and the antibody molecule. Indeed, we wanted to determine if some particles could have a ‘direct effect’ on the recognition molecule: when a particle interacts with the molecule, is there degradation or is the molecule completely insensitive to particle interaction? This approach can allow the identification of particles and energies more deleterious to antibodies (if existing) and the results obtained could help for studying the implementation of biochips on further exploration missions whatever the target object in the Solar System. For instance, it could give precious data on the shielding design that must be developed considering the expected irradiation environment.
To determine the density of antibodies grafted into a well, we used an innovative quantification technique called amino density estimation by colorimetric assay (ADECA) (Coussot et al. Reference Coussot, Faye, Ibrahim, Ramonda, Dobrijevic, Postollec, Granier and Vandenabeele-Trambouze2011a, Reference Coussot, Perrin, Moreau, Dobrijevic, Postollec and Vandenabeele-Trambouzeb; Moreau et al. Reference Moreau, Faye, Baqué, Desvignes, Coussot, Pascal and Vandenabeele-Trambouze2011) that was well adapted to our purpose. The grafting density of antibodies was defined around 8.8 × 1011 antibodies cm−2 with roughly 2.8 × 1011 antibodies on the bottom and 5 × 1011 antibodies on the sidewalls.
The fluence of particles reaching the antibodies was assessed using numerical simulations performed with the Geant4 toolkit. Indeed, considering the geometry of the well, it is obvious that antibodies grafted on the sides do not receive the same fluence of particles as antibodies located at the bottom of the well. With a fluence of 3 × 1012 particles cm−2, the fluence of particles on the sidewalls was derived from the Geant4 simulations to be 2.4 × 10−2 times the total fluence so 7.2 × 1010 particles cm−2. Therefore, we can assess that 41% of antibodies grafted in a well have a significant chance to interact with at least one particle. With this method, direct effects of particles on antibodies can be detected if existing.
Lower- and higher-fluences were also tested in some cases, with for example a fluence of protons ten times lower (3 × 1011 particles cm−2) or a fluence of neutrons ten times higher (3 × 1013 particles cm−2). In these cases, we estimate that 13 and 74% of grafted antibodies interacted with a particle, respectively.
Free antibody samples were prepared at a concentration of 15 × 1016 antibodies well−1. The exact disposition of antibodies into the well is not defined but it is assumed that they form several layers at the bottom of the well during freeze-drying. Therefore it is not possible to determine the number of antibodies that could interact with incident particles since each particle can penetrate in a column of piled antibodies.
Neutron irradiation
Neutron irradiation was performed at the cyclotron of Louvain-la-Neuve, in Belgium. The high flux neutron irradiation facility uses a primary 50 MeV deuteron beam on a beryllium target. The energy spectrum of the outcoming neutron beam is dominated by a peak in the region of 23 MeV. The mean energy of neutrons is 16.56 MeV.
The current was set to 7 µA. Samples were positioned at two different distances so that they received two different fluences. At a 12 cm distance, the fluence was F H = 3 × 1013 neutrons cm−2 and the diameter of the beam was about 4.2 cm for 80% of homogeneity. Whereas at a 40.5 cm distance, the fluence was F L = 3 × 1012 neutrons cm−2 and the diameter of the beam was about 10.2 cm for 80% of homogeneity. Samples were irradiated during approximately 22 min.
Proton irradiation
Proton irradiation was also performed at the cyclotron of Louvain-La-Neuve, on the Light Ion Facility (Fig. 2 Top). This mono-energetic proton beam line can produce up to 109 protons cm−2 s−1 with energies from 10 to 75 MeV (Berger et al. Reference Berger, Ryckewaert, Harboe-Sorensen and Adams1997). The beam diameter is set to 10 cm and a ± 10% of homogeneity is ensured.
Top: proton irradiation using the Light Ion Facility (LIF) at the cyclotron of Louvain-la-Neuve. The source is located on the left in this picture and the samples are placed on the right behind a metal slide with a 10 cm diameter hole. Several removable disks are placed between the source and the samples to allow the modulation of protons energy. Bottom left: picture of the facility at the Institut Bergonié where samples were irradiated with 9 MeV electrons. Bottom right: mobile device developed to ensure the ELISA plate motion during carbon ions irradiation at LNS (Laboratori Nazionali del Sud; Catania).
Three irradiation campaigns took place between June 2010 and 2012. Our samples were irradiated with five different energies: 14.4, 20.9, 25.9, 29.4 and 50.5 MeV. The proton flux was set to 5 × 108 protons cm−2 s−1 so that the irradiations lasted 1 h 40 min to reach the fluence of 3 × 1012 protons cm−2 for all the tested energies and 10 min to reach 3 × 1011 protons cm−2 for 25.9 and 50.5 MeV.
Electron irradiation
Electron irradiation was performed at the Institut Bergonié (Bordeaux, France) (Fig. 2 Bottom Left). The beam was calibrated to deliver 9 MeV electrons and it was scanned through a square collimator of 6 cm side. Samples were positioned at 1 m from the source. The flux delivered by the facility was 200 MU min−1 (Monitor Unit) with 1 MU corresponding to 5.38 × 106 electrons impacting the bottom of the well (Gobet et al. Reference GobetSubmitted; unpublished data). Therefore, to deal with reasonable irradiation durations, we decided to irradiate samples during 70 min corresponding to a fluence of 2.35 × 1011 electrons cm−2.
Carbon ions irradiation
Carbon ions irradiation was performed at the LNS (Laboratori Nazionali del Sud) facility of the INFN (Instituto Nazionale di Fisica Nucleare) in Catania. Samples were presented vertically in front of the beam. A specific mask was designed to fix the ELISA plate containing samples on a mobile device (Fig. 2 bottom right) so that the whole plate could be irradiated at once without any intervention in the irradiation room.
The beam was scanned through a square collimator of 17 mm side. Calibration for the delivered dose has been done by means of a parallel plate ionization chamber. Radiochromic films have also been used for minimizing gaps and overlaps between irradiated areas in order to ensure a homogeneous irradiation of all samples.
The beam delivered 12C ions with an energy of 62 MeV nuc−1. For this experiment, the fluence applied was different from other experiments as it was not reasonable to reach 3 × 1012 carbon ions cm−2 in an adequate delay and safe conditions. Therefore, we decided to study if energetic carbon ions could have an indirect effect on antibodies, i.e. if those particles of such energy could interact with the sample environment so that it could destabilize the whole system and degrade antibodies recognition performances. The fluence was set to 2.16 × 106 particles cm−2 and was determined using results obtained with CREME 96 by Le Postollec et al. (Reference Le Postollec2009a): it corresponds to the flux of 12C 62 MeV nuc−1 ions at 1 A.U. (Astronomical Unit) delivered during 18 months (representing an upper limit for a Mars mission). The irradiation of each square area lasted less than 20 s to reach the requested fluence so that the whole plate was irradiated within about 15 min.
Analysis protocol
Antibodies
After irradiation, analyses were performed in order to define the irradiation effects on the antibody performance. Protocols used here were detailed in previous studies (Baqué et al. Reference Baqué2011a) and are summarized below.
Grafted antibodies were analysed with a direct enzyme-linked immunosorbent assay (ELISA) test (Baqué et al. Reference Baqué2011a). This method, called A2HRP, focuses only on the recognition capability of the antibody's antigen binding site (paratope) and does not give an insight on the degradation of the entire antibody structure (Moreau et al. Reference Moreau, Faye, Baqué, Desvignes, Coussot, Pascal and Vandenabeele-Trambouze2011). Briefly, the number of active antigen binding sites was measured by quantifying the amount of antigen (HRP) specifically retained by the antibodies. Indeed, the amount of HRP could be easily quantified using external standards of free HRP as we have demonstrated that the enzymatic reactivity of HRP was identical for free HRP, or HRP complexed to both free or grafted antibody (Moreau et al. Reference Moreau, Faye, Baqué, Desvignes, Coussot, Pascal and Vandenabeele-Trambouze2011).
Free antibodies were analysed with a competitive ELISA test (Baqué et al. Reference Baqué2011a). Briefly, in micro-well plates with freshly grafted anti-HRP antibodies, a defined amount of HRP is placed in competition with diluted amounts of irradiated samples or controls. After washing, the amount of HRP measured in the micro-well is inversely proportional to the amount of active antibody in the sample. Based on competitive curves, we calculated the half maximal inhibitory concentration (IC50). In our experiment, this concentration represents the amount of competitive antibody that should be added to inhibit 50% of antigen binding to grafted antibodies. Between two competitive experiments, both HRP and grafted antibody concentrations are maintained identical. Thus IC50 values are influenced by the affinity of competitive antibodies for the HRP. If the apparent affinity of competitive antibodies is reduced, then the IC50 measured will increase.
Reference samples
To evaluate the possible irradiation effects on our samples, different references and controls were prepared. Irradiation effect on antibody was evaluated by comparing irradiated samples to non-irradiated controls (NIC). NIC were treated simultaneously and in the same manner as the irradiated samples, though they were not submitted to irradiation. In order to estimate the effects of transport, temperature cycles and light exposure on biochip performances, reference samples were used. These reference samples (R4 °C) were prepared at the same time as irradiated samples and NIC and were stored in the laboratory at 4 °C in the dark until analysis. As described by Baqué et al. (Reference Baqué2011a), all of the antibodies were freeze-dried using a specific buffer, which maintains the anti-HRP antibody recognition capabilities after freeze-drying and during storage to liquid reference levels. Results for grafted antibodies are therefore presented as percentages of active antibodies for more clarity and in order to normalize all acquired data during the several irradiation campaigns. This percentage is calculated by taking the amount of HRP retained by NIC to 100%. NIC and R4 °C were confronted for each campaign to reflect any damage caused by transport, handling etc.
Statistical treatment
Irradiation effects were evaluated by comparing the mean signal values obtained for NIC and for irradiated samples. Thus, Student's t-tests were used to compare irradiated samples distribution and references distribution, taking into account the number of repetitions (from 4 to 18) and the standard deviation (SD) of each distribution. The differences between these two distributions were considered statistically significant with a 95% level of confidence when the calculated P-values were below the 0.05 threshold value.
Results
Grafted antibodies
All the experiments performed on grafted antibodies are summarized in Table 1. This table presents the type, energy and fluence of particles tested. It also specifies the antibody grafting surface density allowing an assessment of the percentage of antibodies receiving at least one particle for each tested fluence. The percentage of antibodies still active after irradiation, calculated against NIC, reveals possible degradation induced only by radiation exposure. Indeed, the effects of transport conditions are evaluated by confronting NIC with reference samples stored in the laboratory (R4 °C), as described in the section ‘Methodology adopted to choose irradiation parameters’. However, as for all the tested conditions NIC proved to be significantly equal to R4 °C (not shown), only irradiation effects are presented here.
Influence of neutron, proton, electron and carbon radiation effects on grafted antibodies recognition capability at different fluences
The percentages of active antibodies were normalized using the non-irradiated controls (NIC) that were thus fixed at 100%. The percentage of antibodies receiving at least one particle was calculated according to the antibody surface density, the tested fluence and the sample geometry.
SD, standard deviation; n is the number of measurements.
P-value <0.05 (in bold) indicate samples that are different to NIC at 95% of confidence.
a Antibody surface density is equal to 8.8 × 1011 Ab cm−2 for all experiments.
Irradiation on the other hand had different effects on the tested antibodies. Indeed, although no effect was detected with neutrons, electrons and 12C, significant effects were observed with protons. Surprisingly, for high fluences, protons between 20 and 30 MeV significantly altered the antibody recognition performances, with losses around 30–35% and P-values between 10−4 and 10−8, but not at lower and higher energies. Similarly, even at a lower fluence 25 MeV protons produced a significant recognition loss, though limited to only 10–20%, whereas at 50 MeV no significant recognition loss was recorded. In our model the antibody surface density was maintained identical for the different exposure experiments therefore only 13% of antibodies should have received at least one particle at the lowest proton fluence against 42% at the highest. The protons’ energy appears thus as a more damaging factor than the fluence, as only a certain energy range (20–30 MeV) produced significant damage to antibodies regardless of the fluence applied. However, by diminishing the ratio between the antibody surface density and the particles’ fluence by a factor 3 (42% against 13% of antibodies receiving at least one particle between high and low fluences, respectively) the effect of irradiation was greatly attenuated for 25 MeV protons (65% against 84% of active antibodies, respectively).
The other tested particles did not induce significant changes in antibody recognition capabilities even at very high neutron fluence (3 × 1013 particles cm−2) or with heavy carbon ions at high energy (62 MeV n−1).
Free antibodies
Free antibodies were irradiated by 25 and 50 MeV protons and 17 MeV neutrons at different fluences (3 × 1011 and 3 × 1012 particles cm−2 for protons and 3 × 1012 and 3 × 1013 particles cm−2 for neutrons). Results are summarized in Table 2. The irradiation effect was estimated following the methodology described in Baqué et al. (Reference Baqué2011a). Briefly, when the half maximal IC50 significantly increases, it indicates that in average, the antibodies have lost recognition capabilities since HRP has only one epitope to which the antibody binds (Moreau et al. Reference Moreau, Faye, Baqué, Desvignes, Coussot, Pascal and Vandenabeele-Trambouze2011).
Influence of neutron and proton irradiation on free-antibody recognition capability at different fluences. IC50 (μg mL−1), half maximal inhibitory concentration
NIC, non-irradiated controls; SD, standard deviation; n is the number of measurements. The percentages of active antibodies were estimated in comparison with NIC.
P-values <0.05 (in bold) indicate samples that are different to NIC at 95% of confidence.
No modification of free antibody recognition capabilities under proton irradiations at 50 MeV was observed. However, at 25 MeV, we highlight here a significant recognition capability loss for free antibodies, leading to a significant increase in IC50 compared with the NIC. The increase in IC50 value indicates that, in average, the antibody activity has been deteriorated by 25 MeV protons irradiation leading to partial or complete antigen recognition site degradation. Based on a simplistic model, which considers that IC50 changes are only linked to a total loss of recognition capability, we can however estimate the percentage of active antibodies compared with NIC as reported in Table 2. A maximum of 50% of antibodies appear to have lost their recognition capability when irradiated with a high fluence of 25 MeV protons. Although the other recorded changes in IC50 values after proton or neutron irradiation appear also quite high, with 20–30% damaged antibodies (most notably after a high neutron flux), they were not significantly different from the controls. These results however point out a high variability in the samples, which can be problematic for repeatability measurements of future space instruments.
Discussion/Conclusion
Based on Monte Carlo simulations of the radiation environment faced by a biochip dedicated to explore Mars’ surface (Le Postollec et al. Reference Le Postollec2009a), our team performed several ground-based irradiation experiments on biochip recognition molecules. Even though protons and neutrons clearly dominate the radiation spectrum during the Earth–Mars transit and on the Martian surface, other particles might be equally deleterious to biological molecules such as the antibodies used in biochips. Furthermore, a wide range of particle energy and fluence can be considered according to the envisaged mission to Mars but also to other planetary bodies of interest in the Solar System. In the present study, the irradiation effects of protons, neutrons, electrons and carbon ions on the recognition capabilities of antibodies were therefore investigated at different energies and fluences. Two antibodies formulations were submitted to irradiation in order to broadly represent any future biochip-based space instruments as both grafted and free antibodies are considered. Our experimental approach consisted of using particle fluences in the same order of magnitude as grafted antibodies surface density in order to measure any damaging effect occurring when a particle interacts with an antibody.
Among the tested particles, only protons significantly altered the antibodies recognition capabilities. These damaging effects were however recorded only for a certain energy range between 20 and 30 MeV at both high and low fluences but confirmed for both formulations (free and grafted antibodies). Indeed, at higher and lower protons energies the antibodies recognition capabilities were not significantly altered. Irradiations of free antibodies lead moreover to a high variability in the estimated recognition capabilities of our antibodies samples.
Therefore, although the energy range of deleterious particles appears quite limited, a biochip instrument performance would not be affected for a typical mission to Mars, as the fluences of particles in this energy range will be significantly lower than the antibody surface density. However, this result underlines that attention must be paid to the ratio between antibody surface density and particles fluences expected for a given mission. The biochip instrument must be designed so that antibody surface density is much greater than incident protons fluence. Instrument shielding and/or antibodies grafting density should be consequently adapted.
In a similar ground-based study performed on five antibodies freeze-dried in different protective molecular matrices, Derveni et al. (Reference Derveni, Hands, Allen, Sims and Cullen2012) pointed out the more damaging role of processing and packaging than irradiation. Using doses of protons and neutrons at high energies (50 and 47 MeV, respectively), comparable with the ones used in the present work, they did not detect any evidence of activity loss due to irradiation for a typical mission dose (1011–1012 protons cm−2 and 107–108 neutrons cm−2). However, using 1013 protons cm−2, most of the antibodies lost their activity. Thanks to these results they suggested that further shielding or alternative radiation protection approaches would need to be considered for long duration missions to other astrobiological targets. Our present work confirms this suggestion. We propose that the ratio between the fluence of protons and the surface density of antibodies has to be much lower than unity to prevent important loss of activity.
The main limitation of ground-based studies is that each constraint is generally studied individually and for a limited period of time that is not representative of a real space mission. In particular, the effect of cosmic rays is generally studied at a given energy (or a limited range of energies) and for one type of particle in a single experiment.
Moreover, additional constraints and hazards are expected for a space instrument. Long term storage, temperature variations, contamination risks, launch, landing and transportations vibrations and shocks should all be taken into account in the design and testing of a space dedicated instrument. For these reasons, a real space exposure of biochip prototypes has been attempted in the past by the LMC team for a short-term mission aboard the BIOPAN platform on a Russian Foton spacecraft (Derveni et al. Reference Derveni, Allen, Sawakuchi, Yukihara, Richter, Sims and Cullen2013) and ground-based and field studies have been performed for the SOLID prototype (Parro et al. Reference Parro2008; Sobrado et al. Reference Sobrado, Martín-Soler and Martín-Gago2014). Furthermore, in the frame of the BiOMAS project, biochip samples are currently exposed to real space conditions inside the EXPOSE-R2 platform of ESA, part of the Photochemistry on the Space Station project, which was installed on the outside of the Zvezda module of the International Space Station (ISS) in August 2014 (Vigier et al. Reference Vigier2013).
The long-duration exposure of the EXPOSE missions (Rabbow et al. Reference Rabbow2009, Reference Rabbow2012, Reference Rabbow2015) range from 12 to 18 months in the LEO environment of the ISS. The radiation environment at this altitude, although not equivalent to interplanetary space or the Martian surface, will allow anyway for a much better estimate of the long-term resistance of immunoassays instruments for space applications.
Nevertheless, due to the high number of potentially hazardous factors encountered during a space mission, ground-based studies are essential to isolate the most damaging ones and thus propose adequate shielding or handling procedures.
Thus, our results from ground-based irradiation campaigns globally indicate that cosmic rays might not alter the final performance of a biochip-based instrument in a typical Martian mission, when antibodies are used as binders to detect the presence or the absence of a target compound. The damaging effects of 20–30 MeV protons recorded in the present study should not however be overlooked and further testing on-ground will be necessary to support and interpret data from real space exposure missions.
Acknowledgements
The research leading to these results has received funding from the European Union Seventh Framework Programme FP7/2007-2013 under Grant Agreement no 262010 – ENSAR. We would like to thank the French National Space Agency (CNES) (Convention number DCT/SI/IM/2009-17733) and the Interdisciplinary CNRS program ‘Environnements Planétaires et Origines de la Vie’ for financial support. We also thank the Louvain-la-Neuve cyclotron facility staff, the Institut Bergonié staff and the staff from the Laboratori Nazionali del Sud of the Instituto Nazionale di Fisica Nucleare for their help during irradiation experiments.
