Introduction
In their overall assessment of the European Red List of freshwater fish, Freyhof & Brooks (Reference Freyhof and Brooks2011) concluded that nearly 80% of native species in Europe are endemic and 37% are threatened, which is high compared to other taxonomic groups (e.g. 23% of amphibians and 15% of mammals are threatened; Freyhof & Brooks, Reference Freyhof and Brooks2011). There is evidence that 13 fish species have already gone extinct in Europe, with five others facing impending extinction. The greatest threats to native European fish are pollution, habitat destruction and invasive alien species (Dudgeon et al., Reference Dudgeon, Arthington, Gessner, Kawabata, Knowler and Lévéque2006). The effects of climate change (e.g. drought) have been shown to drive the deterioration of freshwater ecosystems, and fish living in shallow waters of wetlands are in particular danger (Heino et al., Reference Heino, Virkkala and Toivonen2009; Pratchett et al., Reference Pratchett, Bay, Gehrke, Koehn, Osborne and Pressey2011; Jeppesen et al., Reference Jeppesen, Mehner, Winfield, Kangur, Sarvala and Gerdeaux2012; Ellis et al., Reference Ellis, Stoessel, Hammer, Wedderburn, Suitor and Hall2013). Nonetheless, fish are underrepresented in the conservation literature, with a clear bias in favour of birds and mammals (Seddon et al., Reference Seddon, Soorae and Launay2005; Bajomi et al., Reference Bajomi, Pullin, Stewart and Takács-Sánta2010).
Fen habitats are particularly exposed to environmental regulation by people, and in Europe their area has decreased by 62% to 187,000 km2 since the 1850s (Rosenthal et al., Reference Rosenthal, Hildebrandt, Zöckler, Hengstenberg, Mossakowski, Lakomy, Burfeindt, Schneider-Sliwa, Schaub and Gerold1998; Joosten & Couwenberg, Reference Joosten, Couwenberg, Succow and Joosten2001; Brinson & Malvárez, Reference Brinson and Malvárez2002). In Hungary 97% of fens have been destroyed by regulation of watercourses, draining and ploughing (Tatár, Reference Tatár2010). Small, isolated remnant marshland populations are particularly sensitive to environmental alterations, which often lead to a decrease in genetic diversity and an increase in mortality, as in the case of the European mudminnow Umbra krameri. Freyhof & Brooks (Reference Freyhof and Brooks2011) urged the establishment of Freshwater Key Biodiversity Areas and the development of species protection plans for European freshwater fish species. They also suggested establishing habitat monitoring and ex situ programmes, restrictions on settling of non-native fish species, and revision of the relevant legislation. One of the most important roles of a species protection plan is to identify and rectify factors that caused the initial extinction or decline (Fischer & Lindenmayer, Reference Fischer and Lindenmayer2000; IUCN/SSC, 2013; Cochran-Biederman et al., Reference Cochran-Biederman, Wyman, French and Loppnow2015). Accordingly, we summarize the actions and results of our pilot conservation programme for the European mudminnow (Tatár et al., Reference Tatár, Sallai, Demény, Urbányi, Tóth and Müller2010).
The European mudminnow is small-bodied (7–8 cm), has a short lifespan (4 years), is the only native representative of the Umbridae family in Europe and is a relic and endemic fish species of the Danube catchment area (Gaudant, Reference Gaudant2012). It occurs sporadically along the Danube River between Vienna and the Danube Delta. Some populations live in the lower stretches of the River Dniester but the species’ main range is in the Carpathian Basin (Kottelat & Freyhof, Reference Kottelat and Freyhof2007; Kuehne & Olden, Reference Kuehne and Olden2014). The European mudminnow lives in marshes, fens, vegetated backwaters and channels with clean water (Pekárik et al., Reference Pekárik, Hajdú and Koščo2014). The main threats to the species are habitat loss as a result of dredging of channels, the destruction of river and stream floodplains, the loss of fens and marshes (Wanzenböck, Reference Wanzenböck1995; Kuehne & Olden, Reference Kuehne and Olden2014) and the spread of the invasive Chinese sleeper Perccottus glenii. This voracious competitor and predator of the European mudminnow is expanding its range in Eurasia and has colonized the catchment area of the Danube and Dniester rivers (Reshetnikov & Ficetola, Reference Reshetnikov and Ficetola2011; Reshetnikov, Reference Reshetnikov2013). It is estimated that populations of European mudminnow have declined by > 30% (Freyhof, Reference Freyhof2011). The species is categorized as Vulnerable on the IUCN Red List on the basis of its restricted and fragmented habitat (Freyhof, Reference Freyhof2011) and it also features on the Red Lists of seven European countries (Müller et al., Reference Müller, Balován, Tatár, Müllerné-Trenovszki, Urbányi and Demény2011).
Several successful attempts have been made to conserve populations of the species, primarily by means of habitat restoration and the reintroduction of rescued stocks (Wanzenböck, Reference Wanzenböck1995; Keckeis & Sehr, Reference Keckeis and Sehr2014); for example, in the Slovenian Beloviči backwater populations of European mudminnow, as well as weatherfish Misgurnus fossilis and crucian carp Carassius carassius, increased significantly after restoration of their habitat (Povž, Reference Povž1990). European mudminnow fry were also introduced successfully to waters in Austria (Benesch, Reference Benesch2004) and close to the River Morva in Slovakia, which is part of the species’ native range (Valachovič & Kováč, Reference Valachovič and Kováč1998). However, translocations from Beloviči oxbow to a gravel pit (Povž, Reference Povž1995a) were unsuccessful. After the original population had gone extinct, the NGO Biotica Ecological Society restocked fry into small water bodies in the Lower Dniester area (Aps et al., Reference Aps, Sharp and Kutonova2004). A successful reintroduction was carried out in Fertő-Hanság National Park (north-west Hungary) by translocating wild individuals from other areas (Ambrus & Sallai, Reference Ambrus and Sallai2014; A. Ambrus, pers. comm). Although some of these trials proved to be successful at a local scale, there is no detailed action plan for the conservation of declining populations of the European mudminnow across its diminishing and fragmented range.
Our aim was to develop and test a comprehensive methodology to promote the rescue and strengthening of populations of European mudminnow, for which habitat loss is one of the most important threats (Sallai, Reference Sallai and Barna2005; Kuehne & Olden, Reference Kuehne and Olden2014). Considering the decreased population size, an important challenge is to preserve the species’ remnant genetic diversity, distributed across small and isolated habitat patches (Takács et al., Reference Takács, Erős, Specziár, Sály, Vitál and Ferincz2015). Accordingly, we implemented a pilot experiment that included:
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(1) determining habitat characteristics required by the species, for consideration in the planning of surrogate habitats,
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(2) creating new fen and marsh surrogate habitats to supplement lost native areas and harbour rescued populations (creating new self-sustaining populations in Szada Pilot Area),
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(3) rescuing broodstocks from habitats known to be damaged or exposed to contamination,
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(4) checking the quality of the created habitats before stocking them with European mudminnow,
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(5) propagating and rearing European mudminnow under controlled laboratory conditions for reintroduction and for the conservation of genetic diversity, and
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(6) monitoring the quality of the created habitats after stocking.
Behavioural degradation could be minimized by introducing rescued broodstock to the new surrogate habitats quickly rather than holding them in the laboratory (Hammer et al., Reference Hammer, Barnes, Piller and Sortino2012; Ellis et al., Reference Ellis, Stoessel, Hammer, Wedderburn, Suitor and Hall2013). The key behavioural aspects for the survival of stocked fish are the ability to eat and avoid being eaten. Fish are often necessarily reared on artificial diets because of the cost, limited supply and potential disease risk of wild foods, but this potentially reduces their foraging efficiency in the wild (Hammer et al., Reference Hammer, Barnes, Piller and Sortino2012).
We hypothesized that carefully designed artificial habitats would be appropriate surrogate habitats for the European mudminnow to establish permanent self-sustaining stocks, and that either breeding in the laboratory or reproduction of broodstocks in the artificial habitats would yield enough offspring to reinforce threatened populations or replace extinct ones. To support the assessment of the ecological status of the created habitats, we included in the study two other threatened components of marshland fish assemblages, the weatherfish Misgurnus fossilis and the crucian carp Carassius carassius.
Study area
For habitat construction experiments we selected nine natural lowland sites within Hungary's Carpathian Basin (Fig. 1; Table 1) that had existing or previously known populations of European mudminnow (Sallai, Reference Sallai and Barna2005). The chosen habitats represented the environmental range occupied by the species, and included fens, ponds, slow-flowing and vegetated streams, and canals. Two more sites were added subsequently because of threats to the species: Gőgő-Szenke Stream, which was threatened by anthropogenic pollution, and a fen in the construction area of the South M0 highway bridge (Ráckeve Danube Branch Natura 2000 Site, Czuczor Island), which was about to be filled up as part of the expansion of the highway.
Fig. 1 (a) Sites of surveyed European mudminnow Umbra krameri populations and habitats (Table 1 and Table S2) in Hungary, with numbers of caught individuals and stocked captive-bred individuals. White circles represent extinct populations. The inset shows the numbers of fish caught for propagation and stocked offspring in Illés Ponds I, III, IV, VI of Szada Pilot Area (Table 3). (b) Location of natural and artificial ponds (Pócos and Illés Ponds, respectively) in Szada Pilot Area. *Parent fish were captured in Pócos Pond B (site 9; 6 individuals) and Illés Pond IV (13 individuals). The latter habitat is connected to Pócos Pond B.
Table 1 Fish assemblages and macrophyte coverage recorded during surveys at sites of existing or previously known populations of European mudminnow Umbra krameri in Hungary's Carpathian Basin (Fig. 1), with site number and survey date in parentheses.
1Protected/rare marshland fish species
2Invasive species
The 20 ha Szada Pilot Area (Fig. 1) was chosen for the creation of new and revitalized fen and marsh habitats (Illés Ponds I–VI) based on the following criteria. Firstly, it is a drained wetland area with a few small and isolated native populations of European mudminnow (in Pócos Ponds A and B, sampling sites 8 and 9; Fig. 1). Secondly, the groundwater level does not drop below 1.5 m even during droughts, thus facilitating the creation and maintenance of fen and marsh habitats. Thirdly, this area is adjacent to the EU's Natura 2000 network of protected areas (Natura 2000, 2012), and thus there should be no further risk of significant human impact. Finally, it is close to the laboratory of Szent István University (Gödöllő) where breeding took place.
Methods
Assessment of habitats and new surrogate habitats (Illés Ponds)
The ecological requirements of the European mudminnow were determined based on a comprehensive literature review and field research in native habitats. In total, 11 natural habitats of the species in the three main regions of Hungary were selected at random for surveys, from a habitat list of National Parks (Fig. 1).
Water quality analysis and botanical investigations were conducted between February and September, and hydrobiological surveys were carried out in May and June during 2008–2015. Dissolved oxygen, pH and conductivity were recorded using portable water quality meters (Voltcraft DO-100, PH-100 ATC and LWT-01, Conrad Electronic, Hirschau, Germany) and other chemical properties of the water (phosphate, ammonium, nitrite and nitrate ion concentrations) were measured using a VISOCOLOR® ECO test kit (Macherey-Nagel, Dűren, Germany). Abundance and number of taxa of macroinvertebrates (i.e. food base of fish) were assessed using a kick-and-sweep sampling method (mesh size 950 μm, frame size 25 × 25 cm). Macroinvertebrate samples were preserved in 4% formaldehyde or 96% ethanol prior to identification in a laboratory. Botanical investigations included assessment of the macrophyte coverage and identification of the dominant taxa at each site. Algal or bacterial blooms observed were recorded.
Fish assemblages were sampled during 2008–2012 using electric fishing (IG200, Hans Grassl, Schönau am Königssee, Germany) along randomly selected 150 m long transects. Samples were taken by wading upstream in streams and canals, and covering the whole volume of small, still waters (i.e. ponds and fens; < 500 m3). Captured fish were identified and measured immediately, counted and released. The same procedure and methodology used for natural habitats was also used to monitor the pre- and post-stocking water quality of Illés Ponds.
Creation and monitoring of new surrogate habitats
Based on a literature review and field surveys of the natural habitats of the European mudminnow (Supplementary Tables S1–S5) we designed Illés Ponds in the Szada Pilot Area (Fig. 1). During 2008–2010 we established six groundwater-fed ponds (i.e. Illés Ponds I–VI; each with 50–60 m3 volume, 30–40 m2 surface area, 1–1.5 and 2.5 m mean and maximum depths, respectively) by dredging degraded terrestrial habitats dominated by the invasive plants Solidago spp. Rather than creating one large lake we designed several smaller ponds with a high shoreline-to-surface ratio, which is important for the development of diverse and abundant macrophyte and macroinvertebrate assemblages (Gee et al., Reference Gee, Smith, Lee and Griffiths1997; Cremona et al., Reference Cremona, Planas and Lucotte2008). To increase environmental diversity we created irregular shorelines and bottoms. The ponds were created such that 50–70% of their surface area was in the shade of the surrounding trees and shrubs to prevent excessive warming and algal blooms, which are not tolerated by marshland fish. Shading was increased by introducing macrophytes to the ponds. Aquatic vegetation also decreases the nitrate, nitrite and ammonium content of water, which also inhibits the growth of algae. We planted the ponds with indigenous aquatic macrophytes Ceratophyllum demersum and Lemna minor from a nearby water body; the common reed Phragmites australis was present in the Szada Pilot Area and colonized the littoral of Illés Ponds spontaneously. As we wished to preserve the genetic identity of each of the rescued broodstocks, Illés Ponds were constructed in such a way as to ensure the isolation from each other and from the surrounding aquatic habitats (except Illés Pond IV, which was created as a refuge extension of the over-vegetated and shallow natural Pócos Pond B). Isolation of experimental ponds from surrounding watercourses also prevents immigration of invasive fish.
After their construction Illés Ponds were monitored regularly for water chemistry, macrophytes and macroinvertebrates (Supplementary Tables S1–S5). Following 8–23-month colonization periods, ponds that were considered to be appropriate were stocked with European mudminnow. After stocking, monitoring of ponds was supplemented with seasonal sampling of fish, applying the same method outlined above for small, still water bodies.
Saving threatened stocks, captive breeding and releases
During our field trips and by maintaining contact with other conservationists countrywide we actively searched for situations where populations of European mudminnow were threatened by human activities. In such cases, fish were captured using electric fishing, placed in a plastic barrel filled with oxygenated water and transported to a laboratory at the Szent István University.
In the laboratory broodstocks were held in separate aquaria to preserve their genetic identity for a 2-week acclimatization period prior to attempted breeding using two procedures. The first group was placed in breeding tanks with 15°C water temperature and a photoperiod similar to that of the spawning season, in April; the bottom of the tanks was covered by a green plastic net to prevent cannibalism of eggs and larvae. (Green netting was used for camouflage amidst the aquatic vegetation.) The second group received carp pituitary extract treatment to induce propagation. Larvae were then reared in aquaria and in an artificial 10 m3 pond lined with foil. For more details of propagation and rearing see Müller et al. (Reference Müller, Balován, Tatár, Müllerné-Trenovszki, Urbányi and Demény2011) and Demény et al. (Reference Demény, Müllerné Trenovszki, Tatár, Sipos, Urbányi and Müller2014).
Captured fish, and their laboratory-reared offspring, originating from different populations were stocked in separate Illés Ponds. Offspring from captive breeding and from natural spawning in Illés Ponds were used to supplement populations in native habitats where the parents originated. Laboratory-reared weatherfish and crucian carp were also stocked in Illés Ponds.
Results
Environmental characteristics of European mudminnow habitats
According to published data and the results of our surveys the physical and chemical water quality of natural habitats of the European mudminnow varies widely (Supplementary Table S1). The species’ habitats usually have a low dissolved oxygen concentration; for example, we measured oxygen levels of 0.87 and 0.31 mg l−1 in Pócos Pond A and at Ócsa Landscape Protection Area, respectively. Our field surveys revealed that macrophyte coverage in these habitats can vary widely (0–100%), with a mean value of 61% (Table 1). The European mudminnow generally occurs in shallow (0.5–1.5 m), shaded and often low-volume waterbodies. Data on the abundance of macroinvertebrates in samples from various native habitats are summarized in Supplementary Table S2.
Fish assemblages of European mudminnow habitats
Of the nine investigated sites where the presence of the European mudminnow had been confirmed previously, we found the species only at four (Table 1). We also found the species at the two additional sites threatened by anthropogenic impacts (i.e. the fen at the South M0 Bridge and Gőgő-Szenke Stream). In general, fish assemblages of still-water habitats comprised fewer species (1–4) than those of streams and canals (2–11; Table 1). There was a negative relationship between the occurrence of the European mudminnow and the presence of invasive species. We found the European mudminnow in abundance only at five sites that lacked invasive species or had a low abundance of Prussian carp Carassius gibelio (i.e. Gőgő-Szenke Stream). In contrast, five former habitats of the European mudminnow where the species is no longer present are now populated by invasive fish, including the black bullhead Ameiurus melas, Prussian carp, pumpkinseed Lepomis gibbosus, Chinese sleeper Perccottus glenii and stone moroko Pseudorasbora parva. We found only one exception; the Felső-Tápió stream harboured a small number of European mudminnow as well as an abundance of invasive species. The weatherfish was the most common native species captured over all sites.
Creation and pre-stocking monitoring of surrogate habitats
Pre-stocking water quality, macrophyte and macroinvertebrate (i.e. food base) monitoring (Supplementary Tables S1–S3) revealed that Illés Ponds I, III, IV and VI met the species’ conservation criteria following a short primary succession period, and thus they were assigned for stocking with European mudminnow during 2010–2011. In Illés Pond II there were regular blooms of cyanobacteria, and proliferation of sulphur bacteria was also a common phenomenon. In 2012 we observed high nitrite concentrations (Supplementary Table S1) and blooms of Cladophora sp. in Illés Pond V. Consequently Illés Ponds II and V were excluded from the conservation programme.
Captive breeding and saving threatened stocks
In 2010 a total of 42 adult European mudminnow were rescued and transported to the laboratory from three threatened sites: the Pócos Pond B (site 9), which nearly dried up in that year; the heavily polluted Gőgő-Szenke Stream (site 10); and the fen in the construction area of the South M0 Bridge (site 11; Table 2). Induced propagation with carp pituitary treatment was attempted three times but no larvae were hatched. In contrast, fish that did not receive any treatment formed bonded pairs and laid eggs on the bottom of the breeding tank. From this 864 juveniles were introduced to the Szada Pilot Area and the parents’ original habitat (Table 3). Laboratory-reared juveniles reached a mean standard body length of 4.7 cm in aquaria by early August; they reached a mean body length of 5.5 cm and sexual maturity (females visibly carried developing eggs) in an artificial pond by mid September.
Table 2 Outcome for European mudminnow saved from three sites (Fig. 1), bred in the laboratory and released at three artificial ponds, with capture site, number of individuals captured, mortality during the acclimatization period, number of individuals bred in the laboratory, post-breeding mortality, number of sexually mature individuals released after laboratory breeding, and release date and location. More than half of mortalities were attributable to poor condition, age or disease.
Table 3 Details of releases of European mudminnow offspring of captive bred and nursed stocks.
1 From laboratory breeding of 13 parent fish captured in Illés Pond IV on 21 March 2012 and re-released into the same pond on 21 April 2012
2 Natural offspring of saved adults released to Illés Pond III
3 Csupics Island is a nearby and similar natural habitat to the partially destructed fen at the South M0 Bridge (Czuczor Island).
Stocking surrogate habitats with marshland fish
Environmental assessment of Illés Pond I 14 months after its creation indicated favourable conditions for marshland fish (Tables S1 & S3). However, we had not yet rescued any European mudminnow at that time and therefore we stocked 50 one-summer-old juveniles of weatherfish and crucian carp in the pond in autumn 2009. Test fishing in 2010 revealed that these fish had survived in the pond (Table 4). We subsequently stocked Illés Ponds I, III, IV and VI with European mudminnow. After propagation in the laboratory we released a total of 20 saved adult European mudminnow into Illés Ponds I, III and IV in spring 2010 and summer 2011 (Table 2). Between spring 2010 and summer 2012 we stocked Illés Ponds I, III, IV and VI with 593 laboratory-reared juveniles aged 43–188 days (Table 3). During 2011–2012 we stocked Illés Ponds III and VI with 220 weatherfish and 108 crucian carp juveniles, respectively, which had been bred under laboratory conditions.
Table 4 Results of monitoring in Illés Ponds (Fig. 1), with numbers of European mudminnow, crucian carp and weatherfish, mean recapture rate (no. recaptured relative to no. stocked) and mean coverage of macrophytes. As Illés Pond IV is connected to Pócos Pond B to provide a refugee for natural marsh fish assemblages in dry periods, recapture rate is not relevant. In some cases low water temperature probably decreased the sampling efficiency.
1 257 captured larvae of natural progeny were used to reinforce the threatened parental population (Table 3).
2 Weatherfish larvae released at small size (c. 1 cm) were probably still smaller than the mesh size.
3 On some occasions fish sampling was ineffective because of dense vegetation, and thus these results are incomplete.
4 Illés Pond IV is connected to native Pócos Pond B, and therefore the exact place of spawning cannot be identified.
Post-stocking monitoring of surrogate habitats
Results of water quality and macroinvertebrate assessments following the stocking of Illés Ponds I, III, IV and VI with European mudminnow are in Supplementary Tables S4 and S5. Monitoring revealed that water quality, number of macroinvertebrate taxa, and macroinvertebrate abundance of these ponds are mainly within the range of natural habitats. Test fishing showed that introduced fish survived in their new habitats. Observed mean recapture rates were 17% for European mudminnow, 48% for crucian carp and 13% for weatherfish (Table 4). Natural reproduction of European mudminnow was evident in the year following the first introductions (Table 4). Recruitment was most abundant in the densely vegetated Illés Pond III. Although we released more adults into Illés Pond I (n = 13) than into Illés Pond III (n = 5), reproduction success was weaker in the former, probably because of the low macrophyte coverage.
Reinforcement of natural European mudminnow populations
Given the success of our experiments we were able to reinforce and reintroduce populations of European mudminnow in the threatened habitats where parent fish had originated. Following an administrative measure to eliminate pollution we reintroduced 100 39-day-old and 50 208-day-old laboratory-reared individuals into Gőgő-Szenke Stream in May and October 2010, respectively (Table 3). Laboratory-reared 429-day-old offspring (n = 121) of parents that originated from the fen at the South M0 Bridge were introduced to a similar, nearby habitat, Ráckeve Danube Branch at Szigetcsép (Table 3). Following successful spawning of European mudminnow, offspring were partially harvested from Illés Pond III and 257 individuals of c. 131 and 180 days old were reintroduced to the preserved remnant of their native habitat, the fen at the South M0 Bridge, in August and September 2011, respectively. The threatened population of Pócos Pond B was reinforced indirectly by ensuring its connectivity with Illés Pond IV.
Discussion
Environmental characteristics of European mudminnow habitats
We recorded extremely low dissolved oxygen concentrations in several habitats. This circumstance is tolerated by the European mudminnow, which uses its swim-bladder as an auxiliary breathing organ (Geyer & Mann, Reference Geyer and Mann1939). The ability to survive periods of low oxygen concentrations confers a competitive advantage over other fish species in marsh and fen environments. Pekárik et al. (Reference Pekárik, Hajdú and Koščo2014) found that both the probability of the presence of European mudminnow and its abundance tend to increase with macrophyte coverage. The mean value of macrophyte coverage was 52 and 9% (free-floating and submerged plants, respectively) in the surveyed habitats, which differs from the assessment of Pekárik et al. (Reference Pekárik, Hajdú and Koščo2014), who concluded that the optimal macrophyte coverage is c. 45 and 39%.
Current status of native European mudminnow populations
More than half (five of the nine investigated) of the previously known populations were not found in our surveys, indicating the species may be at considerable risk of extinction. Other studies have reached similar conclusions (Sallai, Reference Sallai and Barna2005; Kuehne & Olden, Reference Kuehne and Olden2014) and identified two main sources of threats. Most of the species’ habitat was lost during the extensive regulation of rivers and wetlands in the 19th and 20th centuries (Takács et al., Reference Takács, Erős, Specziár, Sály, Vitál and Ferincz2015). However, habitat loss is ongoing, as illustrated by the case of the fen at the South M0 Bridge, where construction work related to transport infrastructure and expansion of recreational areas are resulting in the loss of wetland habitats.
The second evident threat is the spread of invasive fish species. Invasive species were abundant in all former habitats of the European mudminnow where we failed to capture any individuals. Conversely, where the European mudminnow was present invasive species were generally absent or occurred only in small numbers. The most voracious invader in these habitats is the Chinese sleeper, which is known to have negative impacts on the European mudminnow (Sallai, Reference Sallai and Barna2005; Reshetnikov, Reference Reshetnikov2013). The European mudminnow may coexist with most invasive fish species (Keresztessy, Reference Keresztessy1995; Povž, Reference Povž1995a) but in the long term it does not survive the settlement of the Chinese sleeper (Sallai, Reference Sallai and Barna2005; Reshetnikov, Reference Reshetnikov2013). Although there are signs of both competition from and predation by other invasive species (Ferincz et al., Reference Ferincz, Staszny, Weipert, Sütő, Soczó and Ács2014), the extent of these relationships has not yet been quantified. It is likely that, at least in some cases, invasive species are not the primary cause of the European mudminnow's population decline. The proliferation of invasive species in wetland habitats could be an indicator of environmental degradation (e.g. regulated water level, dredging of macrophytes, increased nutrient load), and thus their effect on the European mudminnow could be considered to be indirect. Habitats of the European mudminnow generally contain species-poor fish assemblages; among the most common associate species are the weatherfish and the crucian carp (Sallai, Reference Sallai and Barna2005; Pekárik et al., Reference Pekárik, Hajdú and Koščo2014), which are adapted to survive periods of extremely low oxygen concentrations and low water levels (Geyer, Reference Geyer1940; Povž, Reference Povž1995b). Habitat alteration may facilitate the establishment of other species, including invasive fishes and, therefore, it is important to conserve and restore wetland habitats in their original form.
Both refuge fen habitats in the Szada Pilot Area (i.e. Pócos Ponds A and B) contained European mudminnow populations and were free of invasive species, which supports the relevance of this area for a species conservation experiment.
Establishment and pre-stocking monitoring of surrogate habitats
Illés Ponds, created based on our experiences studying native habitats of the European mudminnow, revealed variable environmental characteristics. Although these ponds are located close together in the same degraded wetland landscape, they vary in their water quality, macrophyte flora and macroinvertebrate fauna. Four of the ponds proved to be suitable for marshland fish conservation, being occupied by rich macroinvertebrate, amphibian and reptilian assemblages, including rare and protected species (e.g. the raft spider Dolomedes fimbriatus, the smooth newt Lissotriton vulgaris, the common spadefoot Pelobates fuscus, the grass snake Natrix natrix and the European pond turtle Emys orbicularis).
The creation of several small habitat patches (i.e. ponds) instead of a more extended and connected fen system has several advantages, especially in monitoring and controlling ongoing biological and environmental processes, preventing the spread of disease, and keeping invasive species out of the system. It also facilitates the preservation and maintenance of broodstocks from different populations with unique genetic pools (Marić et al., Reference Marić, Snoj, Sekulić, Krpo-Ćetković, Šanda and Jojić2015; Takács et al., Reference Takács, Erős, Specziár, Sály, Vitál and Ferincz2015).
Pre-stocking monitoring of created habitats is advisable, as habitat quality and prey availability influence the outcome of introductions of freshwater fish (Griffith et al., Reference Griffith, Scott, Carpenter and Reed1989; Ellis et al., Reference Ellis, Stoessel, Hammer, Wedderburn, Suitor and Hall2013; Cochran-Biederman et al., Reference Cochran-Biederman, Wyman, French and Loppnow2015). The unfavourable conditions in Illés Ponds II and V may be attributable to the soil structure and the slow flux of groundwater. Furthermore, shading from riparian vegetation could prohibit the establishment of macrophytes, thus facilitating planktonic eutrophication.
Saving fish from threatened habitats
Our study targeted three threatened populations of European mudminnow: the fen at the South M0 Bridge construction area and the Gőgő-Szenke Stream, both of which had suffered direct anthropogenic impacts, and Pócos Pond B, where habitat loss had occurred as a result of drought. We believe that at least the first two might have gone extinct without our action. Rapid rescue and relocation of broodstocks from these sites to the laboratory and to surrogate habitats created in the Szada Pilot Area ensured the survival of these gene pools.
Rescuing and maintaining broodstocks from threatened populations is a commonly used conservation tool (Hammer et al., Reference Hammer, Barnes, Piller and Sortino2012; Ellis et al., Reference Ellis, Stoessel, Hammer, Wedderburn, Suitor and Hall2013). However, captive maintenance may raise problems of reduced genetic diversity given the small number of parent fish (Philippart, Reference Philippart1995), and behavioural alterations under laboratory conditions (Philippart, Reference Philippart1995; Lynch & O'Hely, Reference Lynch and O'Hely2001). Nevertheless, many wild populations of European mudminnow could be small, especially during droughts. It should be emphasized that many of these isolated populations may represent unique gene pools and therefore important management units (Takács et al., Reference Takács, Erős, Specziár, Sály, Vitál and Ferincz2015).
Captive breeding
Reintroductions of threatened species depend on successful captive-breeding programmes (Witzenberger & Hochkirch, Reference Witzenberger and Hochkirch2011). The majority of freshwater fish species can be propagated using hormone treatments, even out of their reproductive season (Muscalu-Nagy et al., Reference Muscalu-Nagy, Appelbaum and Gospič2011; Zakęś et al., Reference Zakęś, Szczepkowski, Partyka and Wunderlich2013). However, all our attempts to induce controlled propagation of European mudminnow failed. Despite there being no reports of successful induced propagation in this species, this outcome is still surprising, particularly as fish spawned spontaneously in the aquaria under temperature and light conditions similar to those in the spawning season. Spontaneous spawning of European mudminnow has also been observed by other researchers (Geyer, Reference Geyer1940; Povž, Reference Povž1990; Bohlen, Reference Bohlen1995; Kováč, Reference Kováč1997; Müller et al., Reference Müller, Balován, Tatár, Müllerné-Trenovszki, Urbányi and Demény2011; Demény et al., Reference Demény, Müllerné Trenovszki, Tatár, Sipos, Urbányi and Müller2014; Kucska et al., Reference Kucska, Kabai, Hajdú, Várkonyi, Varga and Müllerné-Trenovszki2016). Consequently, laboratory-bred offspring may be used for species conservation purposes but their availability could be limited both in quantity and timing. We obtained a sufficient quantity of larvae from laboratory spawning to stock the Illés Ponds, and the larvae were easily reared to sizes suitable for stocking.
Survival of fish in surrogate habitats
The most critical measure of any surrogate habitat is whether the introduced species can survive and reproduce there (i.e. establish a self-sustaining population; Fischer & Lindenmayer, Reference Fischer and Lindenmayer2000; Cochran-Biederman et al., Reference Cochran-Biederman, Wyman, French and Loppnow2015). Although we only monitored a limited post-stocking period, the results suggest that stocked European mudminnow, as well as weatherfish and crucian carp, survived in the four Illés Ponds that were initially determined to be suitable environments for fish conservation. Reproductive success was also evident in Illés Ponds I and III. In Illés Pond IV we also observed some offspring; however, as this pond is connected to native Pócos Pond B, the exact place of spawning could not be identified. In the case of Illés Pond VI, post-stocking monitoring did not cover a sufficiently long period to evaluate reproductive success. However, it should be emphasized that in densely vegetated habitats such as Illés Ponds, fish sampling is ineffective and results are biased, as electronarcotized individuals, especially small-bodied juveniles, remain hidden among the dense macrovegetation. Furthermore, considering the small size of stocked individuals recapture rates probably significantly underestimated actual survival rates.
Illés Ponds were designed for permanent use, with introduced broodstocks to be maintained without further disturbance except regular monitoring. Long-term monitoring of these populations will ultimately reveal whether our ex situ conservation experiment has been successful; however, benefits of Illés Ponds are already apparent in that we were able to harvest offspring hatched there and reintroduce them to native habitats.
Reinforcement of natural populations
The ideal outcome for a conservation rescue is that threatened and rescued populations recover in their original restored habitat or in an equivalent natural or artificial surrogate habitat within the range of the conservation unit (Fischer & Lindenmayer, Reference Fischer and Lindenmayer2000; Olden et al., Reference Olden, Kennard, Lawler and Poff2011; Ellis et al., Reference Ellis, Stoessel, Hammer, Wedderburn, Suitor and Hall2013). We deemed our efforts to save and conserve populations of European mudminnow as successful when we reintroduced all three threatened populations to their original habitats while preserving their genetic identity.
Conclusion
Populations of European mudminnow have declined or disappeared over most of their native range and are threatened by habitat loss and invasive species. Our pilot programme is the first comprehensive species conservation programme that includes rescuing individuals from threatened populations, captive breeding and rearing, creation of surrogate habitats, introduction of saved and captive-bred stocks and reinforcement of threatened parent populations of European marshland fish. Our experience in the conservation of the European mudminnow has been positive and our results attest to the wide-scale relevance of such complex approaches in preserving other wetland species and biodiversity. However, to facilitate larger-scale conservation actions further research is needed to resolve the problem of reliable induced propagation in this species. In addition range-wide investigations are needed to improve our knowledge of the ecological processes in which the European mudminnow participates and to identify the most threatened populations, habitats for restoration and potential areas for creation of new surrogate habitats. Conservation actions should be synchronized and a range-wide conservation plan should be developed, taking into consideration the genetic guide on relevant conservation and management units, based on information about which populations may be used for recruiting (i.e. serving a pool of parent fish in artificial breeding programmes) and stocking into reconstructed habitats in different geographical areas, and which populations have the genetic integrity to be preserved without mixing them with other populations (Takács et al., Reference Takács, Erős, Specziár, Sály, Vitál and Ferincz2015).
Acknowledgements
We are grateful to Bence Balován (Tavirózsa Association), Zsolt Boczonádi (Szent István University), Eszter Buza (Szent István University), Ferenc Demény (Szent István University), György Hajdú (Umbra Association), Katalin Keresztessy (Szent István University), Sándor Krenedits (Tavirózsa Association), David McFetridge (London School of Business and Finance), Zoltán Sallai (Hortobágy National Park Directorate), Dávid Szőts (Nagy Gáspár City Library), Dr Péter Takács (Balaton Limnological Institute), Dr András Takács-Sánta (Eötvös Loránd University), László Vécsey (Municipality of Szada), Prof. Gábor Vida (Eötvös Loránd University) and Gábor Várbíró (Hungarian Academy of Sciences). We thank Prof. Doug Armstrong (Massey University), Prof. Robin J. Pakeman (James Hutton Institute), Dr Daniel L. Jeffries (University of Lausanne) and Nathan Spillman (Society for Conservation Biology) for their useful comments.
Support from Magyar Telekom Nyrt. helped to start the European Mudminnow Conservation Pilot Programme. Our research was funded by the Hungarian Ministry of Environment and Water and the Hungarian Ministry of Rural Development (Green Source Programme 2008–2009, 2011–2012), the National Innovation Office, and Research Centre of Excellence-9878/2015/FEKUT.
Author contributions
ST conducted the water quality and botanical investigations and planned the habitat reconstruction and assessment. BB assisted with summarizing the programme results and writing the manuscript. AS analysed the water quality and botanical data. BT carried out fish fauna studies and analysed the results. MMT and TM carried out captive breeding and rearing of European mudminnow, European weatherfish and crucian carp. BU analysed the data on the macroinvertebrate and fish fauna. BC and JS carried out surveys of macroinvertebrate fauna.
Biographical sketches
Sándor Tatár is a biologist whose main focus is on the vegetation, hydrobiology and restoration of wetlands. Bálint Bajomi works on the reintroduction of threatened animal species. András Specziár studies the ecology and diversity of fish and chironomid assemblages. Balázs Tóth works on in situ protection and monitoring of fish. Magdolna Müllerné Trenovszki, Béla Urbányi and Tamás Müller work on the ex situ conservation of protected and threatened fish species. Béla Csányi and József Szekeres monitor surface waters in light of the EU Water Framework Directive, and assess the status of their macroinvertebrate, faunal and fish assemblages.
