Poor diet and physical inactivity are widely prevalent risk factors of chronic diseases such as CVD and diabetes(1). However, accurate and reliable measurement of dietary intake and physical activity at the population level is challenging, particularly in developing countries(Reference Rzewnicki, Auweele and De Bourdeaudhuij2, (Reference Craig, Marshall and Sjostrom3).
Various techniques, employing questionnaires, records or objective measurements, have been applied for the assessment of dietary intake and physical activity. However, only a few, such as the doubly labelled water (DLW) method(Reference Coward4), have proved sufficiently precise and unbiased for the estimation of energy expenditure and for the validation of dietary energy intake (DEI)(Reference Seale, Rumpler, Conway and Miles5, (Reference Starling, Matthews, Ades and Poehlman6). Although the DLW method provides an accurate estimation of the mean DEI and physical activity level (PAL), its use in large epidemiological surveys is limited owing to scarcity of the stable isotope and its inhibitory cost(Reference Irwin, Ainsworth and Conway7).
Dietary intake is reported inaccurately, most often under-reported(Reference Hill and Davies8). The DLW method has been used to measure energy expenditure and validate energy intake calculated from food records, diet recalls or diet histories in several studies(Reference Black, Prentice, Goldberg, Jebb, Bingham, Livingstone and Coward9–(Reference Black, Bingham, Johansson and Coward12), thereby revealing a substantial bias towards underestimation of reported energy intake. Under-reporting of energy expenditure is said to be less common than that of DEI(Reference Lof and Forsum13, (Reference Rasmussen, Matthiessen, Biltoft-Jensen and Tetens14), and misreporters of energy intake do not share the same characteristics as misreporters of energy expenditure(Reference Rasmussen, Matthiessen, Biltoft-Jensen and Tetens14). Thus, estimates of PAL, obtained through a physical activity questionnaire, may be used to evaluate the validity of reported energy intake in the same subject and to identify subjects with a tendency to under-report energy intake(Reference Lof and Forsum13). On the other hand, some studies have indicated that self-reported instruments have a tendency towards over-reporting of physical activity(Reference Rzewnicki, Auweele and De Bourdeaudhuij2, (Reference Sallis and Saelens15) owing> to the influence of social desirability and social approval(Reference Adams, Matthews, Ebbeling, Moore, Cunningham, Fulton and Hebert16).
Methods of assessing energy intake and expenditure have been widely studied in the developed world(Reference Racette, Schoeller and Lushner17, (18) while similar information is scarce in the developing world. There is an apparent need to identify feasible methods that offer an acceptable level of accuracy for estimating DEI and PAL at a population level.
Methods
The present study was conducted as part of a larger research project for surveillance of risk factors of chronic diseases in Butajira District, Ethiopia. The district is inhabited predominantly by the ethnic group collectively known as the ‘Guraghe’. Farming and petty trading are the main means of livelihood in the area. Butajira District hosts the Butajira Rural Health Program (BRHP), which is an ongoing demographic surveillance initiated in 1986(Reference Berhane, Wall and Kebede19). Adults in the age group 18–64 years were randomly selected from among the urban and rural populations, using the BRHP sampling frame. Pregnant women were excluded from the analysis.
The study employed a cross-sectional community-based design, where two instruments were concurrently applied on the same study participants and the corresponding estimates of energy intake and expenditure were compared. The test–retest reliability of part of the instruments was also examined on a sub-sample of the study participants.
The survey team, data collectors and supervisors were recruited from local youth who had completed high-school education and spoke one or more of the local dialects in addition to Amharic, the national language in Ethiopia. The team was trained on how to identify eligible study subjects, on basic skills of interviewing and recording data, as well as on proper techniques of anthropometric measurements.
Data were collected through interviews and anthropometric measurements. Questionnaires were prepared to allow the collection of data on ‘habitual’ food intake and usual pattern of physical activity. Thus, study participants were asked to list all the foods and drinks they consume on a usual day. The type and amount of food consumed, the method of preparation (fried, boiled, etc.) and the ingredients (for assorted foods) were queried and recorded in a questionnaire prepared in a table format. The size of household utensils commonly used for serving meals was described by respondents as small, medium or large.
During training of the survey team visits were made to households of volunteer team members, who were also local residents, to observe the common domestic utensils and foods. Pictures of these common types of foods and utensils were taken. The weight of usual sizes (full, half, quarter, etc.) of commonly consumed foods was measured on a few samples. The volume of some household utensils, such as cups, bowls and spoons of common sizes, was also measured. Pictures taken during the visit were used in the training of the survey team.
Participants were asked to list, consecutively, all the activities they undertake in a usual day. The type of activities and the corresponding durations were queried and recorded on the questionnaire. The duration of sleep could be estimated from the usual time they go to bed and get up from bed.
Weight was measured using an ordinary bathroom scale and recorded to the nearest 0·5 kg. Height was measured using a locally produced wooden measuring board and recorded to the nearest 1 cm. Subjects wore minimal (light) clothing and removed their shoes or hats during the measurement. The interview and measurement techniques were standardised through training and pre-testing in the field. The clarity of the questions and their cultural acceptability in the study population were assessed during the pre-test of survey instruments.
Individuals were enrolled into the study on the basis of their informed consent. Ethical conduct was maintained throughout the process of the study.
BMR was estimated separately for males and females across different age groups using the Schofield equation(Reference Schofield20). Reported food intake was converted into DEI using the Ethiopian food composition tables(21). The type of food, its main ingredients and the quantity (as estimated from the weight or volume) were used in the process of estimating energy intake.
Total energy expenditure (TEE) of individuals was estimated from the type and duration of reported activities and their respective BMR. MET (metabolic energy equivalent task) factors were assigned to each type of activity and multiplied by the duration to produce energy expenditure due to each type of activity(Reference Ainsworth, Haskell, Leon, Jacobs, Montoye, Sallis and Paffenbarger22, (Reference Ainsworth, Haskell and Whitt23). A MET factor of 1 was assigned for sleep. TEE was calculated as the sum of all MET due to activity or sleep, during a period of 24 h, multiplied by the BMR of the individual.
PAL was calculated as the ratio of TEE to the estimated BMR (i.e. PAL = TEE/BMR) and food intake level (FIL) was determined as the ratio of DEI to estimated BMR (i.e. FIL = DEI/BMR). The reported dietary intake was evaluated at the group level by comparing the mean DEI against the mean TEE, and at the individual level by determining the cut-off limits for a plausible energy intake (FIL) as described by Goldberg et al.(Reference Goldberg, Black, Jebb, Cole, Murgatroyd, Coward and Prentice24). The Goldberg method was used to calculate the 95 % confidence limits (CL) of FIL assuming a given PAL requirement, below and above which it is unlikely that the mean intake represents either habitual intake for weight maintenance or a random low intake.
Study participants were categorised into low, medium and high activity levels using their estimated PAL and the classification of the FAO/WHO/United Nations University (UNU) Expert Consultation(25), so that different PAL cut-off values would be applied at each level. The Goldberg cut-off values for the 95 % CI of the agreement between FIL and PAL were determined, and the individual FIL was then compared with the respective group PAL cut-off. Under-reporters were defined as individuals with FIL values below the lower PAL cut-off for the 95 % CI of the agreement between FIL and PAL. Acceptable reporters were those with FIL values ranging between the lower and upper limits of the 95 % CI for PAL in the respective group. Those with FIL greater than the upper limit of the 95 % CI of PAL were classified as over-reporters(Reference Goldberg, Black, Jebb, Cole, Murgatroyd, Coward and Prentice24, (Reference Black26).
The following formula(Reference Goldberg, Black, Jebb, Cole, Murgatroyd, Coward and Prentice24, (Reference Black27) was applied:
where PAL is the mean PAL of a group at a given activity level, SD is the number of standard deviations corresponding to the CI chosen (−2 for the lower CL and +2 for the upper CL), S is the overall CV for PAL, taking into account the variability in energy intake and BMR, and n is the number of individuals included.
S is given by the equation:
where 
is the within-subject variation in energy intake, d is the number of days of diet assessment, 
is the within-subject variation in repeated BMR measurements or the precision of estimated compared with measured BMR, and 
is the between-subject variation in PAL.
The values used for each factor were 
(Reference Bingham28, (Reference Nelson, Black, Morris and Cole29), 
(for estimated BMR), 
(Reference Black27), d = 28 d and n = 1, in reference to each individual study participant.
In dietary assessment methods that presume to measure ‘habitual’ intake, such as the dietary history method, on a large sample (>500), it is suggested that the number of days used in the calculation (d) is conventionally taken as 28, because the CI for the PAL changes little beyond this level(Reference Black26, (Reference Black and Cole30).
As the primary purpose of the present study was to examine the validity of the reported DEI and TEE at a population level, the two measurements were compared as recommended by Black(Reference Black27). For studies intending to assess ‘habitual’ DEI at a population level with a sample size greater than 100, Black suggests that the mean FIL may be directly compared with the PAL because in larger studies the 95 % CI is barely different from the PAL chosen for comparison.
Data were analysed using the Statistical Package for the Social Sciences statistical software package version 11 (SPSS Inc., Chicago, IL, USA). Comparison of mean values and proportions were made using Student’s t test and the χ 2 test, respectively. The Bland–Altman plot(Reference Bland and Altman31) was used to plot the difference between DEI and TEE against their average. Although the physical activity questionnaire was used as the reference instrument in the comparison, it was not intended to be a true ‘gold standard’, and thus the Bland–Altman analysis allowed an alternative comparison between the two methods.
Results
A total of 619 adults were enrolled into the study from both rural and urban areas of Butajira District. The majority were in the age group 18–44 years, female, married and Moslem. About half of the study participants were unable to read or write. Farmers, merchants/petty traders and housewives constituted the three main types of occupation among the study population (Table 1).
Sociodemographic characteristics of the study population
Mean weight and height were slightly, but significantly, higher among males than females (P < 0·05 and P < 0·01, respectively). However, mean BMI was comparable between the two sexes, 19·67 kg/m2 in males and 19·57 kg/m2 in females (Table 2). About 37 % of the study participants had BMI < 18·5 kg/m2. BMR, estimated using age- and sex-specific equations, was significantly higher (P < 0·001) in males than in females (Table 2).
Anthropometric characteristics and predicted BMRFootnote *
* BMR was estimated using the Schofield equation(Reference Schofield20).
Assessment of the test–retest reliability of the dietary history questionnaire at an interval of 7 d, in a sub-sample of the study population, yielded a significant correlation (r 2 = 0·56, P < 0·01) between the two measurements, although the mean DEI was significantly lower (−0.59 MJ (−139·82 kcal), t = −2·07, P = 0·041) in the first measurement. The pooled mean within-individual CV in DEI derived from the repeat measurement was 36·2 %.
The reported mean duration of activities was 11·5 (sd 2·3) h in men and 8·8 (sd 3·6) h in women. The mean DEI was significantly higher (P < 0·005) in males, 7·96 (sd 3·34) MJ (1901·6 (sd 798·9) kcal), than in females, 7·20 (sd 2·71) MJ (1719·9 (sd 647·5) kcal). The mean DEI:TEE ratio was similar between men, 0·93 (sd 0·44), and women, 0·94 (sd 0·41). The resulting FIL was not significantly different between males, 1·31 (sd 0·56), and females, 1·39 (sd 0·53). On the other hand, while the mean TEE, 8·89 (sd 2·04) MJ (2124·1 (sd 488.1) kcal), was significantly higher among men, the corresponding PAL, 1·54 (sd 0·37), was significantly higher in females, which might be due to the higher BMR in males. The mean (sd) and 95 % CI for the four parameters are indicated in Table 3.
Distribution of DEI, TEE, FIL and PAL by sex in Butajira District, Ethiopia
DEI, dietary energy intake; TEE, total energy expenditure; FIL, food intake level; PAL, physical activity level.
Based on the PAL classification by FAO/WHO/UNU(25), 76·6 % of the study population fell in the sedentary or light activity lifestyle category, with a PAL < 1·7. The proportion of the population with an active or moderately active lifestyle was 14·2 %, while the remaining 9·2 % of the population were classified as having a vigorous or vigorously active lifestyle. A stratified analysis of PAL classified more women into the vigorously active lifestyle (25·3 % v. 6·1 %) while more men were classified under the sedentary or light activity lifestyle (78·7 % v. 64·5 %). The difference in the distribution of PAL between the two sexes was statistically significant (χ 2 = 37·93, P < 0·001).
Mean TEE and DEI, as well as the resulting PAL and FIL, were compared across different population groups. As presented in Table 4, mean TEE and PAL were significantly higher than DEI and FIL, respectively, among the urban population group. On the other hand, among the rural population, the mean differences between TEE and DEI, as well as between PAL and FIL, were not significantly different from zero.
Comparison of (a) mean TEE and DEI, and (b) mean PAL and FIL, across residence and sex in Butajira District, Ethiopia
TEE, total energy expenditure; DEI, dietary energy intake; PAL, physical activity level; FIL, food intake level; CL, confidence limit.
We also evaluated the reported DEI or FIL at an individual level so as to identify acceptable reporters and differentiate their characteristics from those of possible under-reporters and over-reporters. The findings revealed that 32·3 % of the study participants were under-reporters, while 57·0 % were acceptable reporters and the remaining 10·7 % were over-reporters.
Table 5 indicates the PAL values used to classify study participants into low, medium and high activity levels, the mean PAL of each group, and the lower and upper Goldberg cut-off points calculated for each PAL value.
Goldberg cut-off points and 95 % CL of FIL, which differentiate acceptable reporters from under-reporters and over-reporters
CL, confidence limit; FIL, food intake level; PAL, physical activity level.
*PAL classification modified from FAO/WHO/United Nations University, 2004(25).
†95 % CL calculated using the following assumptions: CV2WEI=23 %, CV2WB=8·5 %, CV2tP=15 %, and assuming 28 d for the dietary history method.
The consistency between the group- and individual-level evaluation, i.e. assessment of the overall bias in reporting DEI at the group level and identifying individual under-reporters, was explored by comparing the mean TEE, DEI, PAL and FIL across under-reporters, acceptable reporters and over-reporters. This revealed that, among the three groups, the most comparable estimates between TEE and DEI, or PAL and FIL, were obtained among acceptable reporters, where the mean differences between the parameters were not significantly different from zero. Acceptable reporters had a mean TEE of 8·21 (95 % CI 8·01, 8·42) MJ (1963 (95 % CI 1914, 2012) kcal) and mean DEI of 8·13 (95 % CI 7·93, 8·34) MJ (1944 (95 % CI 1895, 1993) kcal) (Table 6).
Comparison of (a) mean TEE and DEI, and (b) mean PAL and FIL, among under-reporters and non under-reporters in Butajira District, Ethiopia
TEE, total energy expenditure; DEI, dietary energy intake; PAL, physical activity level; FIL, food intake level.
Acceptable reporters also had the most desired ratio (closest to 1) between mean DEI and TEE, 1·01 (95 % CI 0·99, 1·04), unlike under-reporters (0·53) or over-reporters (1·74). Classification of the study subjects according to their reporting status improved the correlation between DEI and TEE, from r = 0·108 before the classification to r = 0·543 among under-reporters, r = 0·531 among acceptable reporters and r = 0·469 among over-reporters. All of the correlations were significant at P < 0·01.
Figure 1 presents the Bland–Altman plot of the difference between DEI and TEE against the average between DEI and TEE, separately for urban and rural study participants. It is evident from the figure that acceptable reporters are symmetrically distributed (concentrated) along the line representing zero difference between DEI and TEE, and around the average DEI + TEE of 8·37 MJ (2000 kcal). However, under-reporters and over-reporters are asymmetrically distributed below and above the acceptable reporters, respectively.
Bland–Altman plot of the difference between dietary energy intake (DEI) and total energy expenditure (TEE) against the average of DEI and TEE for acceptable reporters (
), over-reporters (
) and under-reporters (
). Horizontal lines represent the mean (±2 sd) for the difference between DEI and TEE. To convert from kcal to MJ, multiply by 4·184 and divide by 1000
Under-reporters were more common among the urban sample, and at low average DEI + TEE values, while over-reporters were more common in the rural sample and at higher average DEI + TEE levels. The plot identified under-reporters at all levels of average energy intake, while over-reporters were mainly concentrated at high energy intake levels.
The observed high level of under-reporting among urban population groups prompted further comparison between the two populations. Thus significant differences (P < 0·001) were also detected in mean DEI and the corresponding FIL between the two groups. The mean DEI in urban areas of Butajira District was 6·55 (95 % CI 6·27, 6·84) MJ (1566 (95 % CI 1498, 1634) kcal), while in the rural areas it was 8·41 (95 % CI 8·06, 8·75) MJ (2009 (95 % CI 1926, 2091) kcal), whereas TEE and PAL were not significantly different between the two groups (P > 0·05). The mean DEI:TEE ratio was also significantly lower (t = −6·93, P < 0·001) among urban residents, 0·82 (sd 0·38), than in rural ones, 1·05 (sd 0·43) (Table 7).
Comparison of DEI, TEE, FIL and PAL by residence in Butajira District, Ethiopia
DEI, dietary energy intake; TEE, total energy expenditure; FIL, food intake level; PAL, physical activity level.
Discussion
We compared energy intake and expenditure from self-reported questionnaires on the basis of the principle of energy balance(Reference Goldberg, Black, Jebb, Cole, Murgatroyd, Coward and Prentice24, (Reference Black27). Under conditions of energy balance energy intake equals energy expenditure, and the measurement of energy expenditure may be used to examine the validity of energy intake(25). As with all self-reported measurements, there were obvious concerns with the accuracy of both instruments. Thus, we employed the Goldberg technique(Reference Goldberg, Black, Jebb, Cole, Murgatroyd, Coward and Prentice24) to classify the study population into low, medium and high activity levels, in order to identify misreporting and improve the validity of estimates of DEI and TEE from the self-reported questionnaires.
The estimated mean DEI in the present study is closely comparable with earlier reports of per capita daily energy intake from urban (7·27 MJ (1738 kcal)) and rural (9·17 MJ (2191 kcal)) areas of Ethiopia in 2000. A similar report for 1996 indicated 8·04 MJ (1921 kcal) for urban and 8·13 MJ (1942 kcal) for rural areas. The reported energy intake for urban centres located close to the present study site varied between 6·14 and 7·06 MJ (1467 and 1688 kcal)(32). The low DEI in the study population is consistent with the high prevalence of chronic energy deficiency or adult undernutrition (37 %) in the area.
Mean DEI was significantly higher in rural residents and males compared with their urban and female counterparts, respectively. The observed difference may be due largely to under-reporting as evidenced by the low FIL (1·18) in urban areas. The mean DEI:TEE ratio (1·05) speaks to a better accuracy of the DEI report among rural populations compared with the much lower ratio (0·82) in urban areas. The difference in mean DEI:TEE ratio between the two population groups was statistically significant (t = −6·93, P < 0·001).
Studies that have compared mean DEI from several methods simultaneously in the same subjects(Reference Livingstone and Black33, (Reference Black34) give inconsistent results concerning the relative accuracy of the different dietary assessment methods. In some the dietary history method provided more accurate mean estimates of DEI than found in diet records(Reference Black, Welch and Bingham35), while the reverse has also been observed in others(Reference Black, Jebb, Bingham, Runswich and Poppitt36).
In a review of eleven studies where reported energy intake was compared with energy expenditure by the DLW method(Reference Livingstone and Black33), the overall mean DEI:TEE values for men and women respectively were 0·87 (sd 0·09) and 0·85 (sd 0·09). The most accurate DEI:TEE ratios (1·06) were reported from studies where food intake was observed. There was no significant difference across the other methods, such as dietary history, weighed record, estimated record, 24 h recall or FFQ. The overall mean DEI:TEE estimated by the different studies, as compared with the DLW method, was 0·86. Another summary of DLW studies with measurements of both DEI and TEE found that, in forty-four studies, the mean DEI:TEE ratio was 0·84, and thirty-five out of fifty-six subgroups had a reported mean DEI:TEE below 0·90(Reference Black34).
Although we have not used the DLW or any other objective measure of TEE in the present study, use of the dietary history questionnaire against the physical activity questionnaire gave DEI:TEE ratio of very high accuracy (1·05) among the rural study participants. This value, along with the ratios of 0·93 in males and 0·94 in females, substantiates comparability of the questionnaires for measuring mean DEI and TEE at the population level. The mean FIL in our study, 1·31 in males and 1·39 in females, was lower than in most reports. In the analysis of nearly 600 DLW measurements the mean PAL in free-living people was greater than 1·55. The range of usual PAL for normally active persons was estimated to range from 1·3 to 2·0 in women and 2·4 in men(Reference Black and Cole30).
Application of different PAL cut-off points at three activity levels classified close to 57 % of the study population as acceptable reporters of DEI, while about 36 % were under-reporters. Black(Reference Black26) affirmed that the use of cut-off values depending on individual level of physical activity provides a better definition of under-reporters. The Goldberg technique is said to have poor sensitivity for defining misreporters at the individual level. Moreover, due to the wide confidence limits, it will only allow identification of extreme degrees of misreporting(Reference Black27). Thus the present study might have not identified all possible under-reporters of DEI.
The level of under-reporting in the current study population is comparable with other reports. In a review of twenty-two studies with measurements of TEE by the DLW method, the proportion of acceptable reporters was 62 %, while 34 % were under-reporters and 4 % were over-reporters(Reference Black26). A few studies conducted in developing countries have reported rates of under-reporting using the FFQ: 22·5 % in men and 38·6 % in women in Jamaica(Reference Mendez, Wynter, Wilks and Forrester37), 43 % in South Africa(Reference MacIntyre, Venter and Vorster38).
Evaluation of the accuracy of reported DEI aims to attain a DEI:TEE ratio of 1. Thus population groups with ratios closest to 1 could be regarded as providing valid reports. In the present study, acceptable reporters, identified through the application of PAL cut-offs, provided the most desired DEI:TEE ratio 1·01, which further substantiates agreement between the methods of evaluation applied at the group and individual levels. The improved correlation between reported DEI and TEE following the classification by reporting status provides further evidence for the importance of the Goldberg method.
The Bland–Altman plot(Reference Bland and Altman31) was in agreement with the other approaches of comparison employed in the present study, graphically revealing the distinct distribution of acceptable reporters from the under-reporters and over-reporters. The plot illustrated that under-reporters were more concentrated at lower average DEI levels while over-reporters were more common at higher levels of DEI. The plot visually reaffirms the ability of the Goldberg technique we employed to differentiate different types of misreporters of DEI.
In the present study mean DEI among acceptable reporters was markedly higher than the overall mean before the classification. This finding, of a higher proportion of under-reporters, signifies that use of a dietary history questionnaire without an in-built validation component could bias the outcome of studies which examine the association between diet and diseases, leading to flawed interpretation.
In addition to systematic misreporting, unacceptably low DEI might result from erroneous estimation of the amount of food consumed, or while converting food intake into nutrient intake. Although Ethiopia is one of the few African countries with a national food composition table, not all food items reported in the study area could be obtained in the same composition in the tables. Errors in the process of data collection might have also contributed to misreporting.
Errors could also be introduced while assigning MET values to a given activity type. The Compendium of Physical Activities(39) was intended primarily for use in epidemiological studies to standardise the assignment of MET intensities in physical activity questionnaires. Individual variations in energy expenditure for the same activity could result in inaccurate assignment of MET values as the compendium does not account for differences in biological, geographic or environmental conditions in which the activities are performed. However, we assume that such errors may not markedly alter the population mean values as they are likely to be distributed randomly.
Neither energy expenditure nor BMR was measured objectively in the present study. Thus, the lack of biomarkers, such as the DLW method, is an apparent limitation. On the other hand, the stated limitation was the basis for attempting to produce more practical methods with reasonable accuracy and reliability. Our study design did not allow us to quantify or account for misreporting of physical activity, which might have been under-reported or over-reported. Thus correlated error, which is common feature of concurrent comparison studies, is a possible limitation in our study too.
The questionnaire-based assessment of habitual dietary intake and physical activity offers an inexpensive and practical alternative for estimating DEI and PAL of populations in low-income settings. As energy intake and expenditure are interdependent behaviours, the assessment of dietary intake and physical activity should be linked to one another so as to enable evaluation of the energy balance and cross-examination of the validity of each with the other.
Acknowledgements
Financial support for this study was made available by the WHO, Geneva, and through a special grant from the Swedish Council for Social and Work Life Research (FAS), No. 2003-0075. The Butajira Rural Health Program and study participants residing at the demographic surveillance site are greatly acknowledged.
