Introduction
Carotid intima-media thickness (cIMT) is a marker of pathological arterial wall changes (arterial injury)(Reference Mitchell, Korcarz and Zagzebski1–Reference Sirtori3). Often cIMT is referred to as subclinical atherosclerosis(Reference Blekkenhorst, Lewis and Bondonno4), although this is controversially discussed(Reference Raggi and Stein2,Reference Sirtori3) . Increased cIMT is a considerable public health concern(Reference Song, Fang and Wang5), and it has been estimated that the worldwide prevalence of increased cIMT (≥1⋅0 mm) is >25 % in individuals aged 30–79 years, equivalent to >1 billion individuals(Reference Song, Fang and Wang5).
Most frequently, cIMT is assessed in the common carotid artery(Reference Shah6). Common cIMT (ccIMT) is an established risk marker for cardiovascular disease (CVD) such as myocardial infarction, sudden cardiac death(Reference Suzuki, Wang and Wilsdon7) and stroke(Reference Kumar, Sharma and Misra8). Positive correlations have been shown between ccIMT and calcification scores of the coronary arteries and the aorta(Reference Gałąska, Kulawiak-Gałąska and Chmara9) as well as between ccIMT and epicardial adipose tissue(Reference Qian, Sun and Jiang10).
The prospective assessment of ccIMT in clinical trials offers the possibility to test the effect of the intervention on the arterial structure, and this constitutes a more direct measure of artery health compared to serological markers, while at the same time avoiding invasive, complicated and very expensive procedures(Reference Mitchell, Korcarz and Zagzebski1,Reference Sirtori3,Reference Katakami, Matsuoka and Shimomura11) . A recent meta-analysis of clinical trials, including dietary interventions, has shown that changes in ccIMT progression are able to predict changes in CVD risk, that even relatively small changes are relevant, and that ccIMT progression can thus be considered a suitable parameter for intervention studies(Reference Willeit, Tschiderer and Allara12).
Current evidence indicates that a dietary pattern with a strong emphasis on healthy plant-based components, a high water intake and lower intakes of unhealthy plant-based foods and of meat is associated with lower ccIMT(Reference Blekkenhorst, Lewis and Bondonno4,Reference Yang, Lin and Liu13–Reference Zuo, Tang and Xiong15) .
Thus, the objective of the present study was to test if a healthy lifestyle intervention would beneficially affect ccIMT within 1 year (among other CVD risk markers(Reference Koeder, Kranz and Anand16)).
Materials and methods
Study design
The study was a non-randomised controlled trial, and ccIMT was assessed at baseline, 6 months and 1 year. The study had been planned with a duration of 2 years, but due to the COVID-19 pandemic we did not include the 1½-year time point (uneven time delays between groups; included in sensitivity analyses) or the 2-year time point (no assessment in the control group).
The intervention consisted of a lifestyle programme, and the control group received no intervention. Randomisation was not feasible, as described previously(Reference Koeder, Hahn and Englert17). Both groups were recruited in two separate and comparable small towns in order to keep the participants of the control group unaware of the lifestyle recommendations given to the intervention group. The intervention group was recruited at a public market (February 2018) and by word of mouth, while the control group was recruited at a local public event (September 2018). The funding was provided for a specific time period, at relatively short notice and there were insufficient resources (time and staff) to recruit and start both study arms at the same time. Therefore, the intervention group started 6 months earlier (April 2018) than the control group study arm (October 2018), with equivalent follow-up intervals. The study was registered in the German Clinical Trials Register (DRKS; reference: DRKS00018775; www.drks.de).
Participants
For the intervention and control groups, a total of 114 and 87 participants, respectively, were recruited. Participants were mostly middle-aged and elderly individuals from the general population in rural northwest Germany. As a community-based intervention, the only inclusion criteria were the physical and mental ability to participate and to be ≥18 years old. This study was conducted according to the guidelines laid down in the Declaration of Helsinki and all procedures involving human subjects were approved by the ethics committee of the Medical Association of Westphalia-Lippe and of the University of Münster (Münster, Germany; reference: 2018-171-f-S; approved 4 April 2018). Written informed consent was obtained from all subjects.
Lifestyle programme
The lifestyle intervention has been described previously(Reference Koeder, Hahn and Englert17). In brief, the intervention included an intensive phase (first 10 weeks; 14 seminars and 8 workshops) and a less intensive phase (monthly seminars). The focus of the intervention was on four areas of lifestyle change: a healthy, predominantly plant-based diet (strongest emphasis), physical activity, stress management and social health. Dietary recommendations were to consume more healthy plant foods (fruit, vegetables, whole grains, legumes, including soya foods, nuts, seeds and healthy oils) and to reduce the intake of unhealthy plant foods (added sugars, salt, refined grains, alcohol excess) and of animal-source foods (particularly meat)(Reference Satija, Bhupathiraju and Spiegelman18). Participants also received a healthy lifestyle handbook, a recipe booklet and a laminated information sheet with an overview of the lifestyle recommendations(Reference Koeder, Hahn and Englert17) (Supplementary material).
Assessment of parameters
Measurements of ccIMT were conducted in accordance with the Mannheim consensus(Reference Touboul, Hennerici and Meairs19), as described previously(Reference Koeder, Hahn and Englert17) (Supplementary material). Dietary intake was assessed with semi-quantitative 3-d food protocols. Adherence to dietary recommendations was assessed with the plant-based diet index (PDI), healthful PDI (hPDI) and unhealthful PDI (uPDI)(Reference Satija, Bhupathiraju and Spiegelman18) (as described previously(Reference Koeder, Kranz and Anand16)). As the association of the intakes of potatoes, fish, eggs and dairy with ccIMT are less certain, whereas hPDI includes these food groups as negatives, we also conducted a post hoc analysis with a modified hPDI (excluding the food groups potatoes, fish, eggs and dairy). Physical activity (in categories) and socio-demographic parameters were assessed using questionnaires.
Study hypotheses
In terms of mean and max ccIMT, the study hypotheses were that the intervention would significantly decrease mean and max ccIMT (within-group and compared to control; from baseline to 1 year and from baseline to 1½ years). The two main hypotheses were regarding the between-group changes (mean and max ccIMT: 1-year changes). Any detected differences in the secondary end points mean and max ccIMT are considered exploratory.
Sample size calculation
The primary outcome parameter of the study was body weight(Reference Koeder, Kranz and Anand16), for which a sample size calculation was conducted, and a further sample size calculation was conducted for the secondary parameter ccIMT (as described previously(Reference Koeder, Hahn and Englert17)).
Statistical analyses
Between-group differences in baseline characteristics were assessed using Fisher's exact test for categorical variables and independent t test and Mann–Whitney U test for normally and non-normally distributed continuous variables, respectively (as described previously(Reference Koeder, Hahn and Englert17)). To evaluate within-group changes, paired t test and Wilcoxon signed-rank test were used for normally and non-normally distributed data, respectively. Correlations were assessed with Spearman's rho correlations. All tests were two-sided.
To evaluate between-group differences in 1-year trajectories of ccIMT, a repeated measures analysis of covariance (ANCOVA) was used, with baseline ccIMT and potential confounders (especially age and sex) as covariates. Holm–Bonferroni correction was conducted to adjust for multiple comparisons. As it has been shown that baseline ccIMT is inversely associated with ccIMT change(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20) and ccIMT may thus more strongly decrease in those with high baseline ccIMT(Reference Murie-Fernandez, Irimia and Toledo21), subgroup analyses were conducted, including only participants with high baseline mean ccIMT (≥0⋅800 mm). Analyses were based on unimputed data (complete case analysis, CCA). In sensitivity analyses, imputed data (last observation carried forward, LOCF) were used. Sensitivity analyses using log-transformed (lg10) ccIMT values were also conducted. All analyses were conducted using IBM SPSS Statistics (Version 25.0. Armonk, NY).
Results
Baseline characteristics
For a total of 126 participants (intervention: 71; control: 55), ccIMT values were available for all three measurement time points (baseline, 6 months and 12 months). The flow of participants through the study is shown in Fig. 1.
Flowchart of participants through the study.
At baseline, the intervention group had a significantly higher age and educational level (Table 1). Baseline hPDI was also significantly higher in the intervention group (by 4⋅3 [95 % CI 2⋅0, 6⋅5] portions/day; P < 0⋅001), while PDI and uPDI were not significantly different between groups. Frequency of intense physical activity was higher in the control group (P = 0⋅031) when assessed by categories, but physical activity (gentle, moderate and intense) was not significantly different between groups when assessed by minutes/week.
Baseline characteristics of evaluable participants (CCA)
Values are means ± sem except for qualitative variables which are expressed as n (%).
CCA, complete case analysis; ccIMT, common carotid intima-media thickness; BMI, body mass index; WC, waist circumference; BP, blood pressure; RHR, resting heart rate; TC, total cholesterol; LDL-C, LDL cholesterol; non-HDL-C, non-HDL cholesterol; REM-C, remnant cholesterol; HDL-C, HDL cholesterol; TAG, triacylglycerols; hs-CRP, high-sensitivity C-reactive protein; Hcy, homocysteine; PDI, plant-based diet index; hPDI, healthful PDI; uPDI, unhealthful PDI; sem, standard error of the mean; IN, intervention; CON: control.
P-values in bold are values below 0.05.
* P-value for between-group comparisons by:
a Fisher's exact test (two-sided).
b Independent t test (two-sided).
c Mann–Whitney U test (two-sided).
TC, measured LDL-C, non-HDL-C, REM-C, HDL-C, TAG, glucose, HbA1c, insulin: n 70 (IN); calculated LDL-C, PDI, hPDI, uPDI: n 70 (IN), n 54 (CON); hs-CRP: n 54 (IN), n 36 (CON); Hcy: n 57 (IN), n 54 (CON); Marital status, educational level: n 54 (CON).
Furthermore, a higher percentage of participants with a history of cancer (intervention: 9⋅9 %; control: 0 %; P = 0⋅018) and a higher percentage of participants with a family history (parents) of myocardial infarction or stroke (intervention: 56⋅3 %; control: 36⋅4 %; P = 0⋅031) were observed in the intervention group.
At baseline, there were no significant between-group differences in terms of ccIMT and a variety of other CVD parameters (Table 1). Similarly, there were no significant between-group differences in terms of alcohol intake frequency or the percentage of participants with (based on baseline values) hypertension, high values for total cholesterol (TC), LDL cholesterol (LDL-C; measured and calculated), non-HDL cholesterol (non-HDL-C), triacylglycerols (TAG), glucose or HbA1c or who had low HDL cholesterol (HDL-C). Furthermore, there were no significant between-group differences in terms of the percentage of those with a history of stroke, with a family history (siblings, grandparents) of myocardial infarction or stroke, or with any of a large variety of diagnosed disease conditions (hypertension, dyslipidaemia, heart disease, stroke, peripheral artery disease, diabetes, retinopathy, peripheral neuropathy, diabetic foot, kidney disease, allergies, gastrointestinal disease, thyroid disease, depression, rheumatoid arthritis, chronic pain, lung disease, bone disease as well as ‘other disease’ and ‘free of diagnosed disease’). There were no significant differences in any of the baseline characteristics (as listed in Table 1) between the group of participants with complete ccIMT data and with incomplete ccIMT data (P > 0⋅07).
Seminar attendance
Compliance in the intervention group, defined as seminar attendance during the 10-week intensive phase of the lifestyle intervention, was relatively high: 60 out of the 71 evaluable participants (84⋅5 %) attended ≥10 (out of 14) seminars.
Repeatability of ccIMT measurements
Repeatability (within-assay precision) of the two repeated measurements of mean and max ccIMT was good at all time points (mean ccIMT: r > 0⋅94; max ccIMT: r > 0⋅88). Mean differences in repeated measurements were small for both mean ccIMT (between 0⋅000 and 0⋅005 mm) and max ccIMT (between 0⋅003 and 0⋅007 mm).
Mean ccIMT changes from baseline to 1 year
From baseline to 1 year, mean ccIMT significantly increased in both the intervention (0⋅026 [95 % CI 0⋅012, 0⋅039] mm; P = 0⋅001; n 71) and control group (0⋅045 [95 % CI 0⋅033, 0⋅056] mm; P < 0⋅001; n 55; Fig. 2; Supplementary Table S1). The 1-year trajectory of mean ccIMT was significantly lower in the intervention group compared to control (between-group difference: –0⋅012 [95 % CI –0⋅022, –0⋅002] mm; P = 0⋅022; adjusted for baseline mean ccIMT). This result remained significant after Holm–Bonferroni correction. This result was also confirmed when adjusting for baseline mean ccIMT, age and sex (P = 0⋅038; Fig. 2; Supplementary Table S1) or additionally for educational level, history of cancer, family history (parents) of myocardial infarction or stroke, and baseline hPDI (P = 0⋅045). Furthermore, this result was confirmed after adjusting (in addition to baseline mean ccIMT, age and sex) for baseline smoker status and alcohol intake (P = 0⋅040), for changes in smoker status and alcohol intake (P = 0⋅028), for baseline glucose, HbA1c, systolic BP and pulse pressure (P = 0⋅029), for baseline homocysteine (Hcy; P = 0⋅041) or for changes (Δ[baseline, 1 year]) in diastolic BP (P = 0⋅039). Apart from baseline mean ccIMT, none of the covariates had a significant influence on the models. Using log-transformed covariates confirmed the results.
1-year mean and max ccIMT trajectories. Values are means and 95 % confidence intervals (adjusted for baseline). Whole group: (a and d) (IN: n 71; CON: n 55); subgroup with baseline mean ccIMT <0⋅8 mm: (b and e) (IN: n 53; CON: n 43); subgroup with baseline mean ccIMT ≥0⋅8 mm: (c and f) (IN: n 18; CON: n 12); P-values for between-group difference in 1-year trajectories of ccIMT (by ANCOVA; adjusted for baseline): (a) P = 0⋅022; (b) P = 0⋅970; (c) P < 0⋅001; (d) P = 0⋅117; (e) P = 0⋅965; (f) P = 0⋅023. ccIMT, common carotid intima-media thickness; IN, intervention group; CON, control group.
However, in a sensitivity analysis comparing the 1½-year trajectories of mean ccIMT, the between-group difference was non-significant (–0⋅010 [95 % CI –0⋅022, 0⋅003] mm; P = 0⋅119; adjusted for baseline; intervention: n 63; control: n 47; Supplementary Table S2). Similarly, a sensitivity analysis with imputed data (LOCF) demonstrated no significant between-group difference in 1-year trajectories of mean ccIMT (P = 0⋅815; adjusted for baseline; intervention: n 101; control: n 75; Supplementary Table S3). A sensitivity analysis with imputed data (LOCF) comparing the 1½-year trajectories also attenuated the results (P = 0⋅853; adjusted for baseline). In addition, the favourable effect of the intervention was also attenuated in sensitivity analyses using log-transformed (lg10) mean ccIMT values (1-year trajectories: P = 0⋅051; 1½-year trajectories: P = 0⋅277; adjusted for baseline).
Max ccIMT changes from baseline to 1 year
From baseline to 1 year, max ccIMT non-significantly increased in the intervention group (0⋅006 [95 % CI −0⋅015, 0⋅028] mm; P = 0⋅165; n 71) but significantly increased in the control group (0⋅034 [95 % CI 0⋅015, 0⋅053] mm; P = 0⋅001; n 55; Fig. 2; absolute values at each time point are can be found in Supplementary Table S1). The 1-year trajectory of max ccIMT was not significantly different between the intervention and control groups (between-group difference: −0⋅013 [95 % CI −0⋅030, 0⋅003] mm; P = 0⋅117; adjusted for baseline max ccIMT). This between-group difference remained non-significant after adjusting for baseline max ccIMT, age and sex (P = 0⋅098; Fig. 2; absolute values at each time point can be found in Supplementary Table S1). In contrast, the 1-year trajectory of max ccIMT was significantly lower after adjusting for baseline max ccIMT, age, sex, educational level, history of cancer, family history (parents) of myocardial infarction or stroke, and baseline hPDI (P = 0⋅041) or for baseline max ccIMT, age, sex and changes in smoker status and alcohol intake (P = 0⋅045). This result was again attenuated when adjusting (in addition to baseline max ccIMT, age and sex) for baseline smoker status and alcohol intake (P = 0⋅093), for baseline TAG, glucose, HbA1c, systolic BP, pulse pressure and waist circumference (P = 0⋅061), for baseline hs-CRP (P = 0⋅154), for baseline Hcy (P = 0⋅093) or for changes (Δ[baseline, 1 year]) in body weight and BMI (P = 0⋅164). Apart from baseline max ccIMT, none of the covariates had a significant influence on the models. Using log-transformed covariates confirmed the results.
Similarly, this result was confirmed by a sensitivity analysis comparing the 1½-year trajectories of max ccIMT (−0⋅014 [95 % CI −0⋅034, 0⋅006] mm; P = 0⋅164; adjusted for baseline; Supplementary Table S2), a sensitivity analysis with imputed data (LOCF; P = 0⋅756; adjusted for baseline; Supplementary Table S3) as well as a sensitivity analysis with imputed data (LOCF) comparing the 1½-year trajectories of max ccIMT (P = 0⋅846; adjusted for baseline). Similarly, this result was confirmed in sensitivity analyses using log-transformed (lg10) max ccIMT values (1-year trajectories: P = 0⋅200; 1½-year trajectories: P = 0⋅295; adjusted for baseline).
Mean ccIMT changes from baseline to 1 year (subgroup analysis)
In a subgroup analysis including only participants with mean ccIMT ≥0⋅800 mm, from baseline to 1 year, mean ccIMT non-significantly decreased in the intervention group (−0⋅016 [95 % CI −0⋅050, 0⋅017] mm; P = 0⋅311; n 18) and significantly increased in the control group (0⋅065 [95 % CI 0⋅033, 0⋅096] mm; P = 0⋅001; n 12; Fig. 2; Supplementary Table S4). The 1-year trajectory of mean ccIMT was significantly lower in the intervention group than in control (between-group difference: −0⋅051 [95 % CI −0⋅075, −0⋅027] mm; P < 0⋅001; adjusted for baseline mean ccIMT). This result remained significant after adjusting for baseline mean ccIMT, sex and age (P < 0⋅001; Fig. 2; absolute values at each time point can be found in Supplementary Table S4). Due to the low number of cases in the subgroup, only these covariates were adjusted for. However, in sensitivity analyses, further covariates were added: the between-group difference remained significant after adjusting for baseline mean ccIMT, age, sex, educational level, history of cancer, family history (parents) of myocardial infarction or stroke and baseline hPDI (P < 0⋅001). Similarly, this result was confirmed when adjusting (in addition to baseline mean ccIMT, age and sex) for baseline smoker status and alcohol intake (P < 0⋅001), for changes in smoker status and alcohol intake (P < 0⋅001), for baseline glucose, HbA1c, systolic BP and pulse pressure (P < 0⋅001), for baseline Hcy (P < 0⋅001) or for changes (Δ[baseline, 1 year]) in diastolic BP (P < 0⋅001). Apart from baseline mean ccIMT, age and sex, none of the covariates had a significant influence on any of the models. Using log-transformed covariates confirmed the results.
Furthermore, this result was confirmed in a sensitivity analysis comparing the 1½-year trajectories (P = 0⋅001; adjusted for baseline; Supplementary Table S5), a sensitivity analysis with imputed data (LOCF) comparing the 1-year trajectories (P < 0⋅001; adjusted for baseline; intervention: n 23; control: n 15; Supplementary Table S6), and a sensitivity analysis with imputed data (LOCF) comparing the 1½-year trajectories (P = 0⋅002; adjusted for baseline; P < 0⋅001, adjusted for baseline, age and sex).
In addition, the favourable effect of the intervention was confirmed in sensitivity analyses using log-transformed (lg10) mean ccIMT values (1-year trajectories: P < 0⋅001; 1½-year trajectories: P = 0⋅001; adjusted for baseline). Furthermore, a sensitivity analysis with a cut-off value of 0⋅790 mm (the 75th percentile of baseline mean ccIMT in our study population) confirmed the results (intervention: n 19; control: n 12; between-group difference: −0⋅059 [95 % CI −0⋅082, −0⋅035] mm; P < 0⋅001; adjusted for age and sex).
Max ccIMT changes from baseline to 1 year (subgroup analysis)
In the same subgroup, from baseline to 1 year, max ccIMT non-significantly decreased in the intervention group (−0⋅023 [95 % CI −0⋅071, 0⋅025] mm; P = 0⋅327; n 18) and non-significantly increased in the control group (0⋅041 [95 % CI −0⋅020, 0⋅102] mm; P = 0⋅168; n 12; Fig. 2; absolute values at each time point can be found in Supplementary Table S4). The 1-year trajectory of max ccIMT was significantly lower in the intervention group than in control (between-group difference: −0⋅046 [95 % CI −0⋅085, −0⋅007] mm; P = 0⋅023; adjusted for baseline max ccIMT). Adjusting for baseline max ccIMT, age and sex confirmed this result (P = 0⋅003). In sensitivity analyses, this result was confirmed when adjusting (in addition to baseline max ccIMT, age and sex) for educational level, history of cancer, family history (parents) of myocardial infarction or stroke and baseline hPDI (P = 0⋅007), for baseline smoker status and alcohol intake (P = 0⋅001), for changes in smoker status and alcohol intake (P = 0⋅004), for baseline TAG, glucose, HbA1c, systolic BP, pulse pressure and waist circumference (P = 0⋅003), for baseline hs-CRP (P = 0⋅027), for baseline Hcy (P = 0⋅008) or for changes (Δ[baseline, 1 year]) in body weight and BMI (P = 0⋅001). Apart from baseline max ccIMT, age and sex, none of these covariates had a significant influence on any of the models. Using log-transformed covariates confirmed the results.
A sensitivity analysis comparing the 1½-year trajectories of max ccIMT attenuated the result when adjusting for baseline max ccIMT (P = 0⋅061; adjusted for baseline; Supplementary Table S5) but confirmed the result when adjusting for baseline max ccIMT, age and sex (P = 0⋅011; Supplementary Table S5). This result was also confirmed by a sensitivity analysis with imputed data (LOCF) comparing the 1-year trajectories (P = 0⋅017; adjusted for baseline; Supplementary Table S6) and a sensitivity analysis with imputed data (LOCF) comparing the 1½-year trajectories of max ccIMT (P = 0⋅023, adjusted for baseline; P = 0⋅002, adjusted for baseline, age and sex).
In addition, the favourable effect of the intervention was also confirmed in sensitivity analyses using log-transformed (lg10) max ccIMT values (1-year trajectories: P = 0⋅004; 1½-year trajectories: P = 0⋅020; adjusted for baseline and age). Furthermore, a sensitivity analysis with a cut-off value of 0⋅790 mm (the 75th percentile of baseline mean ccIMT in our study population) confirmed the results (intervention: n 19; control: n 12; between-group difference: −0⋅065 [95 % CI −0⋅104, −0⋅026] mm; P = 0⋅002; adjusted for age and sex).
Changes in diet scores and physical activity from baseline to 1 year
Compared to control, in the intervention group, the 1-year trajectories of PDI and hPDI were higher by 2⋅7 (95 % CI 1⋅7, 3⋅6) food portions/day and 3⋅9 (95 % CI 2⋅7, 5⋅0) food portions/day, respectively, while the 1-year trajectory of uPDI showed a decrease of −2⋅7 (95 % CI −3⋅7, −1⋅7) food portions/day (between-group differences: P < 0⋅001; adjusted for baseline). Sensitivity analyses confirmed that the favourable dietary changes were maintained at 1½ years (between-group differences: P < 0⋅001; adjusted for baseline).
A post hoc analysis showed that in the intervention group the 1-year trajectory of a modified hPDI (excluding the food groups potatoes, fish, eggs and dairy) was higher by 3⋅7 (95 % CI 2⋅7, 4⋅6) food portions/day (compared to control; between-group difference: P < 0⋅001; adjusted for baseline). These results were maintained at 1½ years (between-group differences: P < 0⋅001; adjusted for baseline).
At the food group level, results confirmed that participants of the intervention group were adhering to the recommendations, with significantly increased intakes (1-year trajectories) of fruit, vegetables, whole grains, legumes and nuts (approximately half a food portion/day higher, compared to control) as well as small increases in vegetable oil and fish intake. Conversely, in the intervention group, decreased intakes of meat, sweets/desserts and refined grains (approximately −0⋅3 to −0⋅4 portions/day) were observed, with small decreases also in the intakes of margarine, eggs and miscellaneous animal-source foods (compared to control). There were no significant between-group differences regarding the intakes (1-year trajectories) of tea, coffee, fruit juice, potatoes, sugar-sweetened beverages, animal fats, dairy or alcohol. No significant between-group differences were observed for the 1-year trajectories of intense, moderate or gentle physical activity (P > 0⋅09). This was confirmed for the 1½-year trajectories of physical activity (P > 0⋅10).
Bivariate correlations of changes (Δ[baseline, 1 year]) in ccIMT and other markers
Bivariate correlations of changes in mean and max ccIMT with changes in other CVD markers as well as changes in dietary scores are shown in Table 2. For mean ccIMT change, a significant (although weak) correlation was only observed with changes in diastolic BP (Table 2). For max ccIMT change, significant correlations were only observed for changes in body weight, BMI and uPDI (Table 2). In addition, mean ccIMT change inversely correlated with baseline mean ccIMT (r = −0⋅179; P = 0⋅044), and max ccIMT change inversely correlated with baseline max ccIMT (r = −0⋅259; P = 0⋅003).
Bivariate correlations of changes (Δ[baseline, 1 year]) in ccIMT and other markers
Correlations coefficients: spearman's rho; ccIMT, common carotid intima-media thickness; BMI, body mass index; WC, waist circumference; BP, blood pressure; RHR, resting heart rate; TC, total cholesterol; LDL-C, LDL cholesterol; non-HDL-C, non-HDL cholesterol; REM-C, remnant cholesterol; HDL-C, HDL cholesterol; TAG, triacylglycerols; hs-CRP, high-sensitivity C-reactive protein; Hcy, homocysteine; PDI, plant-based diet index; hPDI, healthful PDI; uPDI, unhealthful PDI.
P-values in bold are values below 0.05.
In the subgroup of participants with baseline mean ccIMT ≥0⋅800 mm, changes in uPDI positively correlated with changes in mean ccIMT (r = 0⋅370; P = 0⋅048) and max ccIMT (r = 0⋅539; P = 0⋅003) [n 29], while no significant correlations between changes in mean or max ccIMT and changes in PDI, hPDI or CVD markers were observed.
A post hoc analysis showed that changes in the modified hPDI (excluding the food groups potatoes, fish, eggs and dairy) inversely correlated with max ccIMT change (r = −0⋅229; P = 0⋅012). This result was confirmed in the subgroup of participants with high baseline ccIMT (r = −0⋅460; P = 0⋅012). Mean ccIMT change did not significantly correlate with changes in modified hPDI.
Mean and max ccIMT changes stratified by baseline risk factors
1-year trajectories of mean ccIMT and max ccIMT did not significantly differ between men and women (P = 0⋅313; P = 0⋅208) or between participants with or without overweight (P = 0⋅365; P = 0⋅707) or with or without obesity (P = 0⋅940; P = 0⋅938). Similarly, 1-year trajectories of mean ccIMT and max ccIMT did not significantly differ by smoker status (P = 0⋅467; P = 0⋅449), categories of alcohol intake (P = 0⋅547; P = 0⋅591), marital status (P = 0⋅921; P = 0⋅931) or educational level (P = 0⋅578; P = 0⋅579; all adjusted for baseline).
Discussion
The present study had the aim of examining the effect of a community-based lifestyle intervention on changes in mean and max ccIMT (among other CVD markers(Reference Koeder, Kranz and Anand16)). While the intervention resulted in significant dietary improvements, in line with the recommendations given, physical activity did not significantly increase over the course of 1 year. Previously we reported that, with all participants included, the intervention had no significant effect on ccIMT within 6 months in the whole study group but that in a subgroup of participants with high baseline ccIMT (mean ccIMT ≥0⋅800 mm) the intervention resulted in significantly lower (more favourable) mean and max ccIMT changes compared to control(Reference Koeder, Hahn and Englert17). The present 1-year analyses (and 1½-year sensitivity analyses) confirm this result of significantly slowed down mean ccIMT and max ccIMT progression in the subgroup with high baseline ccIMT. This result is in line with the results of the PREDIMED-Navarra study, a 1-year controlled intervention with a traditional Mediterranean diet, which showed significant effects on ccIMT only in the subgroup with high baseline ccIMT (mean ccIMT ≥0⋅9 mm)(Reference Murie-Fernandez, Irimia and Toledo21). Comparable subgroup results from other studies seem to not have been published(Reference Agewall, Fagerberg and Berglund22).
A recent meta-analysis suggests that every 0⋅010 mm reduction in mean ccIMT progression can reduce CVD event risk by 10–15 %(Reference Willeit, Tschiderer and Allara12). Therefore, the between-group difference (−0⋅051 [95 % CI −0⋅075, −0⋅027] mm) observed in the high-baseline subgroup of the present study appears to be clinically relevant. In contrast to the previously reported 6-month results(Reference Koeder, Hahn and Englert17), with all participants included, the present 1-year analysis showed a significantly lower (slowed down) mean ccIMT progression in the intervention group. However, this between-group difference (−0⋅012 [95 % CI −0⋅022, −0⋅002] mm) was small and was attenuated in sensitivity analyses comparing the 1½-year trajectories. Nevertheless, it can be hypothesised that the clinical benefit of this effect may increase over time, if participants maintain the achieved lifestyle changes in the future(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20,Reference Maiorino, Bellastella and Petrizzo23) .
To date, only a small number of controlled trials have assessed the effect of a combined lifestyle intervention (including diet and other lifestyle factors) on ccIMT(Reference Agewall, Fagerberg and Berglund22,Reference Vamvakis, Gkaliagkousi and Lazaridis24–Reference Wildman, Schott and Brockwell28) , and only two of these trials were conducted with generally healthy participants(Reference Okada, Maeda and Tatsukawa27,Reference Wildman, Schott and Brockwell28) : in a suburban population in Japan, Okada et al. observed no significant effect of a 2-year lifestyle modification on mean ccIMT change (compared to control; dietary recommendations were based on the goals of the National Cholesterol Education Program; <30 % total fat, <7 % saturated fat and <200 mg dietary cholesterol per day)(Reference Okada, Maeda and Tatsukawa27). Similarly, in a study with perimenopausal women in the United States, Wildman et al. observed no significant effect of a 4-year diet (≤25 % total fat, ≤7 % saturated fat, ≤100 mg dietary cholesterol and 1300 kcal/d) and exercise intervention on mean ccIMT change (compared to control)(Reference Wildman, Schott and Brockwell28). In terms of diet-only interventions (i.e. interventions focused solely on a change in dietary pattern), no controlled trials assessing ccIMT change seem to have been conducted with generally healthy participants. Thus, our study appears to be the first controlled trial (lifestyle or dietary) to have shown a significant intervention effect on ccIMT in a community-based sample of mostly generally healthy participants from the general population.
Apart from our study, only one controlled trial appears to have assessed the effect of a combined lifestyle intervention including a strong focus on a predominantly plant-based diet on ccIMT(Reference Aldana, Greenlaw and Salberg25). In the present study, Aldana et al. observed no effect of a 1-year intervention including a very low-fat plant-based diet (Ornish Program) on ccIMT in patients with clinically confirmed coronary artery disease (compared to usual care)(Reference Aldana, Greenlaw and Salberg25). In contrast to our study, healthful plant-based high-fat foods (such as nuts, extra virgin olive oil or cold-pressed rapeseed oil) were not recommended in the Ornish Program(Reference Aldana, Greenlaw and Salberg25), whereas more recent evidence suggests that these foods may not just improve cardiovascular health in general(Reference de Souza, Dehghan and Mente29) but may also beneficially affect ccIMT(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20,Reference Murie-Fernandez, Irimia and Toledo21) . In terms of diet-only interventions, five controlled trials have assessed the effect of moving towards a more plant-based diet on ccIMT: four of these studies used a Mediterranean diet (in Spain(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20,Reference Murie-Fernandez, Irimia and Toledo21,Reference Sala-Vila, Romero-Mamani and Gilabert30) and Italy(Reference Maiorino, Bellastella and Petrizzo23)), and one study used a Mediterranean-like diet (in Norway(Reference Ellingsen, Seljeflot and Arnesen31,Reference Hjerkinn, Abdelnoor and Breivik32) ). All of these studies were conducted with participants at higher CVD risk, with four out of these five studies showing a significant favourable effect on ccIMT(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20,Reference Murie-Fernandez, Irimia and Toledo21,Reference Maiorino, Bellastella and Petrizzo23,Reference Ellingsen, Seljeflot and Arnesen31,Reference Hjerkinn, Abdelnoor and Breivik32) . In contrast, two controlled trials observed that a low-carbohydrate diet had no significant effect on ccIMT in type 2 diabetes patients after 1 year (United States(Reference Bhanpuri, Hallberg and Williams33)) and 1½ years (Taiwan(Reference Chen, Huang and Chen34)), respectively. As no comparable studies could be identified, our study appears to be the first controlled trial (lifestyle or dietary) which tested the effect of recommending a predominantly plant-based diet (centred around fruit, vegetables, whole grains, legumes, nuts/seeds and healthy oils) on ccIMT in a study sample of mostly generally healthy participants(Reference Ellingsen, Seljeflot and Arnesen31).
Similarly, our study appears to be the first controlled trial (lifestyle or dietary) to assess correlations between ccIMT changes and changes in PDI, hPDI and uPDI as well as between ccIMT changes and Hcy changes. Apart from a dietary intervention study by Petersen et al. (Reference Petersen, Clifton and Blanch35), our study also appears to be the only controlled trial (lifestyle or dietary) to have correlated ccIMT changes with hs-CRP changes. While we observed that uPDI change positively correlated with max ccIMT change (and in the subgroup analyses with both mean and max ccIMT changes), we did not observe significant correlations of ccIMT change with changes in Hcy or hs-CRP. As in our study, Petersen et al. found no significant correlation of mean ccIMT change with hs-CRP change(Reference Petersen, Clifton and Blanch35). The positive correlations of uPDI change with changes in mean ccIMT (r = 0⋅370) and max ccIMT (r = 0⋅539), which we observed in the high-baseline subgroup, indicate that the favourable effects on ccIMT were partially mediated by the observed decrease in uPDI. The results do not indicate a strong role of PDI or hPDI changes in the observed favourable effects. However, the significant inverse correlation of a modified hPDI (excluding the food groups potatoes, fish, eggs and dairy) with max ccIMT change which we observed indicates that an increased intake of healthful plant foods and a decreased intake of certain animal-source foods may also contribute to lower max ccIMT progression, although we observed a significant beneficial effect on max ccIMT change only in the subgroup analysis.
Among diet-only interventions, Jimenez et al. and Maiorino et al. observed a favourable effect on mean ccIMT in their intervention groups following a traditional Mediterranean diet after 5 and 7 years (coronary heart disease patients; Spain(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20)) and after 4 and ~8 years (type 2 diabetes patients; Italy(Reference Maiorino, Bellastella and Petrizzo23)), respectively. It should be noted that both studies compared their intervention groups to control groups following healthy low-fat diets(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20,Reference Maiorino, Bellastella and Petrizzo23) , which may underestimate(Reference Julibert, Del Bibiloni and Tur36) but may also overestimate the effect (e.g. Jimenez et al. observed a decrease in Mediterranean diet adherence in the low-fat control(Reference Jimenez-Torres, Alcalá-Diaz and Torres-Peña20)). In addition to the significant effects at 4 and ~8 years, Maiorino et al. observed a non-significant, favourable effect on mean ccIMT change at 2 years (between-group difference: P = 0⋅050(Reference Maiorino, Bellastella and Petrizzo23)), which indicates that longer study durations may increase the likelihood of observing significant effects on ccIMT. While in our study dietary improvements (compared to control) were similarly maintained at 1 year and 1½ years, it should be considered that in lifestyle and dietary interventions, adherence to recommendations may decrease over time, especially in generally healthy participants, and that dietary changes may occur in the no-intervention control group. Furthermore, our study indicates that dropout rates can be high in study populations of generally healthy individuals, potentially making long-term (≥4 years)(Reference Maiorino, Bellastella and Petrizzo23) follow-up less feasible. We observed that the small but significant intervention effect on mean ccIMT at 1 year (P = 0⋅022) became non-significant at 1½ years (P = 0⋅119), which may have been influenced by the lower number of participants available at 1½ years. In their study with type 1 and type 2 diabetes patients in Australia, Petersen et al. were able to demonstrate a significant favourable effect of repeated counselling from a dietitian on mean and max ccIMT after 1 year (compared to usual diet)(Reference Petersen, Clifton and Blanch35). Petersen et al. observed that mean ccIMT change was ~0⋅016 mm lower in the intervention group (compared to control)(Reference Petersen, Clifton and Blanch35), whereas our study found that mean ccIMT was lower by 0⋅012 mm in the intervention group. While the dietary instructions given to the intervention group by Petersen et al. were to increase the intakes of fruit (not fruit juice), vegetables and dairy (milk or yoghurt, not cheese) and at 3 months increased intakes of fruit (+179 g/d), vegetables (+46 g/d) and yoghurt (+38 g/d) were observed (compared to control), these increases were not maintained at 1 year(Reference Petersen, Clifton and Blanch35). In comparison, we observed increased intakes (1-year trajectories) of about half a food portion/day for fruit, vegetables, whole grains and legumes (equivalent to an increase of about 50–75 g/d each) as well as additional dietary changes (including a reduction in the intakes of meat, refined grains and sweets) in the intervention group (compared to control).
Our subgroup analyses confirm that significant effects on ccIMT may be more easily demonstrated in individuals with higher baseline ccIMT(Reference Murie-Fernandez, Irimia and Toledo21), and such higher ccIMT values are more likely to be present in study samples of individuals with confirmed CVD risk factors such as hypercholesterolaemia(Reference Ellingsen, Seljeflot and Arnesen31) or diabetes(Reference Maiorino, Bellastella and Petrizzo23,Reference Petersen, Clifton and Blanch35) . Nevertheless, it is of high public health relevance to develop tools (such as our intervention programme) which already initiate CVD prevention measures in individuals who are still at low to moderate CVD risk(Reference Nieto-Martínez, González-Rivas and Mechanick37). Consequently, it appears justifiable to conduct further lifestyle/dietary interventions assessing ccIMT change in generally healthy participants. It should also be considered that with participants at higher CVD risk a control group for which no effect (no intervention) or a lower effect can be expected may be ethically problematic(Reference Morain38). Like Petersen et al. (Reference Petersen, Clifton and Blanch35) (and no other controlled lifestyle/dietary trial), our study showed a significant effect on mean ccIMT change after 1 year. At a follow-up of <1 year, significant favourable effects of lifestyle/dietary pattern modification on ccIMT change (compared to control) have only been shown in type 2 diabetes patients (after 6 months; South Korea)(Reference Kim, Lee and Kang26) as well as in our subgroup analysis at 6 months(Reference Koeder, Hahn and Englert17).
A cut-off value for mean ccIMT of 0⋅8 mm, as used in our study, has been widely utilised as a threshold for describing what constitutes elevated mean ccIMT values(Reference Connors, Flores-Torres and Stern39). It has furthermore been proposed that the age-, sex- and race-specific 75th percentile of mean ccIMT (derived from large cohort studies) should be used as a cut-off value(Reference Mitchell, Korcarz and Zagzebski1,Reference Müller-Scholden, Kirchhof and Morbach40) . In our study population (evaluable participants; n 126), the 75th percentile of baseline mean ccIMT was 0⋅786 mm, which confirms the usefulness of 0⋅800 mm as a cut-off value in our study. Sensitivity analyses with a cut-off value of 0⋅790 mm confirmed the results.
Only a small number of controlled trials have tested the effect of exercise-only interventions on ccIMT(Reference Hetherington-Rauth, Magalhães and Júdice41–Reference Farahati, Hosseini and Moazzami46). One small study demonstrated a significant favourable effect of exercise training on ccIMT (compared to control)(Reference Hetherington-Rauth, Magalhães and Júdice41,Reference Magalhães, Melo and Correia43) , and one other study observed a significant beneficial effect only in a subgroup analysis of patients without identified carotid plaques(Reference Byrkjeland, Stensæth and Anderssen42). The other studies did not demonstrate significant effects compared to control.
Age-related ccIMT increase is partly mediated by increased sympathetic nerve activity in vascular smooth muscle(Reference Holwerda, Luehrs and DuBose47). Psychological stress is associated with increased sympathetic activity(Reference Holwerda, Luehrs and Gremaud48), blood pressure(Reference Liu, Li and Li49) and ccIMT(Reference Wiernik, Lemogne and Thomas50), and this highlights the importance of psychological stress management as a component of healthy lifestyle recommendations(Reference Conversano, Orrù and Pozza51,Reference Stier-Jarmer, Throner and Kirschneck52) . In the present study, we observed a significant reduction in body weight in the intervention group (compared to control). Weight loss is associated with decreased sympathetic activity(Reference Costa, Moreira and Moreira53) and decreased ccIMT(Reference Altin, Erol and Aydin54), and in the present study a positive correlation was observed between changes in body weight and changes in max ccIMT (P = 0⋅020) and mean ccIMT (P = 0⋅066). However, in the high-baseline subgroup, changes in body weight did not significantly correlate with changes in mean ccIMT (P = 0⋅387) or max ccIMT (P = 0⋅640). Similarly, adjusting for body weight or BMI changes did not significantly influence the ANCOVA models (P > 0⋅69; unpublished results). This indicates that body weight reduction was not a main driver of the favourable effects on ccIMT which were observed in the subgroup.
Strengths and limitations
A strength of the present study is the use of a no-intervention control group, a strict standardised measurement protocol(Reference Mitchell, Korcarz and Zagzebski1,Reference Touboul, Hennerici and Meairs19,Reference Frigerio, Werba and Amato55) , several follow-up time points, high repeatability(Reference Connors, Flores-Torres and Stern39) and having all ccIMT measurements conducted by the same technician and with the same device(Reference Mitchell, Korcarz and Zagzebski1,Reference Müller-Scholden, Kirchhof and Morbach40) .
Two relevant limitations are the non-randomised design and that the intervention study arm started 6 months earlier than the control group (same follow-up durations). While our findings in the subgroup with high baseline ccIMT seem robust, residual confounding may have remained, particularly since our trial was non-randomised and, as a community-based study, the study sample was non-homogeneous(Reference Willeit, Tschiderer and Allara12,Reference Welch, Antoine and Berta56) . However, baseline characteristics indicate that both study groups were comparable. Furthermore, seasonal changes may have influenced the results, for example by way of improved vitamin D status during the summer(Reference Säidifard, Tangestani and Djafarian57). However, in the subgroup, results of the 6-month(Reference Koeder, Hahn and Englert17), 1-year and 1½-year analyses consistently demonstrated lower mean and max ccIMT trajectories in the intervention group. This indicates that these results are not strongly confounded by seasonal effects. A further limitation is that the relatively small sample size did not allow for a complex statistical analysis of all potential confounders.
Conclusion
The results indicate that healthy lifestyle changes, as they were addressed in the Healthy Lifestyle Community Programme (cohort 2), can effectively reduce mean and max ccIMT if baseline ccIMT is above a value of 0⋅800 mm, indicating an elevated risk. The observed favourable effect of the intervention in participants with high baseline ccIMT likely constitutes a true deceleration of mean and max ccIMT progression. These results appear to be robust and are likely applicable to similar populations. Although in the present study most participants did not have elevated baseline ccIMT values, we still observed a significant and relevant beneficial intervention effect on ccIMT in the analysis including all participants. While the clinical benefit of the observed effect is likely greater in those with elevated ccIMT, it is equally an advantage to maintain normal ccIMT values within the normal range for as long as possible, if one takes ccIMT as a predictor of CVD risk. In the subgroup of individuals with low baseline ccIMT (<0⋅800 mm), lifestyle changes alone may not be sufficient or the lifestyle changes observed in the present study may not have been substantial enough to significantly improve ccIMT in the short term. As mean and max ccIMT are secondary end points, the results should be considered exploratory.
Supplementary material
The supplementary material for this article can be found at https://doi.org/10.1017/jns.2022.46
Acknowledgements
We would like to thank all participants as well as Alwine Kraatz who took part in the investigation, Norman Bitterlich for his help with statistical analysis, Dominik Zeljko for conducting the ccIMT measurements and Reinhold Gellner for his valuable contributions in the application process for ethical approval and his medical advice during the study.
This work was conducted as part of the project ‘münster.land.leben’ and was funded by the German Federal Ministry of Education and Research (BMBF) and the Joint Science Conference (GWK) within the programme ‘Innovative Hochschule’ (H.E., grant number: 03IHS062A). The funders had no role in the study design, data collection, analysis or interpretation, writing of the report or the decision to publish the findings.
C. K.: conceptualisation, methodology, validation, formal analysis, investigation, data curation, writing – original draft, writing – review and editing, visualisation, project administration; S. H.: conceptualisation, methodology, validation, investigation, data curation, writing – review and editing, project administration; R.-M. K.: conceptualisation, methodology, validation, investigation, data curation, writing – review and editing, project administration; C. A.: conceptualisation, methodology, validation, investigation, data curation, writing – review and editing, project administration; D. A.: investigation, data curation, writing – review and editing; N. S.: conceptualisation, methodology, investigation, data curation, writing – review and editing, project administration; A. H.: conceptualisation, methodology, formal analysis, writing – original draft, writing – review and editing, supervision; H. E.: conceptualisation, methodology, investigation, writing – original draft, writing – review and editing, project administration, supervision, funding acquisition.
There are no conflicts of interest.
Open access
