Addressing the health-related consequences of obesity among racial–ethnic minorities is a primary focus of Healthy People 2020, especially among Hispanic adults where the prevalence is approaching 50 % and is considerably higher than among non-Hispanic whites(1). Further compounding the problem, Hispanic adults typically present higher body fat percentage (BF %) values than non-Hispanic black and non-Hispanic white adults, even at similar BMI values across the lifespan(Reference Heymsfield, Peterson and Thomas2). This is problematic since BMI-based body fat equations have been developed in non-Hispanic populations(Reference Jackson, Stanforth and Gagnon3–Reference Deurenberg, Andreoli and Borg5). Despite these growing concerns and increasing obesity trends, valid and reliable body composition assessment methods in Hispanic populations are extremely scarce(Reference Stolarczyk, Heyward and Goodman6) and hence need further development.
Traditional body composition methods such as underwater weighing (UWW) and air displacement plethysmography (ADP) employ a two-compartment (2C) model approach, which divides the body into fat and fat-free masses (FM and FFM, respectively)(Reference Heymsfield, Lichtman and Baumgartner7,Reference Heymsfield and Lichtman8) . A limitation of a 2C model is the assumption of FFM characteristics, which assumes that aqueous content (TBWFFM) and bone mineral (MoFFM) comprise 73·8 and 5·6 %, respectively, of FFM(Reference Brozek, Grande and Anderson9). Moreover, these assumptions are based upon three white male reference cadavers, which is problematic since body composition is known to vary between and within populations(Reference Brozek, Grande and Anderson9,Reference Nickerson, Tinsley and Fedewa10) . To reduce the potential limitations of the 2C model approach, researchers and clinicians often use a combination of body composition assessment techniques to more accurately measure the components of FFM.
Multi-compartment models, such as the four-compartment (4C) model, can more accurately measure body composition by further accounting for individual variations in total body water (TBW) and bone mineral content (BMC)(Reference Wang, Deurenberg and Guo11–Reference Wang, Pi-Sunyer and Kotler13). Despite acceptance of the 4C model, the administration of this technique is not practical outside of research settings due to the need to acquire body volume (BV) via UWW or ADP (4C-UWW and 4C-ADP, respectively). The administration of UWW and ADP can be difficult for individuals who are (1) hydrophobic, (2) claustrophobic or (3) who experience mobility limitations such as elderly and obese populations(Reference Lee and Gallagher14,Reference McCrory, Gomez and Bernauer15) . Collectively, these issues have led to the development of alternative approaches for the measurement of BV, which include the use of dual-energy X-ray absorptiometry (DXA).
There are multiple DXA-derived BV equations available for the estimation of BV(Reference Smith-Ryan, Mock and Ryan16–Reference Wilson, Fan and Shepherd18). However, the use of a DXA-derived BV equation in a 4C model should be carefully selected, as the most commonly used DXA BV equations were developed utilising a Hologic(Reference Smith-Ryan, Mock and Ryan16,Reference Wilson, Fan and Shepherd18) or a GE-Lunar Prodigy DXA scanner(Reference Wilson, Strauss and Fan17). Previous research has shown that the two DXA BV equations derived from Hologic scanners produce significantly different body composition metrics (e.g. BF %) when employed in a 4C model(Reference Tinsley19). Moreover, the Hologic DXA-derived BV equations yield inaccurate volume estimates when used in 4C models in participants scanned using GE-Lunar Prodigy and GE-Lunar iDXA(Reference McLester, Nickerson and Kliszczewicz20,Reference Nickerson, Tinsley and Esco21) . These findings highlight that the DXA-derived BV equations are probably not interchangeable when utilising devices that differ from the scanner used to develop regression coefficients.
Concerns with current DXA-derived BV equations include the use of ADP as a criterion to develop each equation(Reference Smith-Ryan, Mock and Ryan16–Reference Wilson, Fan and Shepherd18). UWW has traditionally been accepted as the ‘gold standard’ for BV assessments(Reference Brozek, Grande and Anderson9,Reference Siri22,Reference Heymsfield23) . Further, research has demonstrated differences between UWW and ADP for the estimation of body density (Db) and BF %(Reference Collins, Millard-Stafford and Sparling24,Reference Vescovi, Hildebrandt and Miller25) . As a result, the error associated with ADP might be embedded in currently existing DXA-derived BV equations, which would reduce accuracy when used in a 4C model. Finally, each DXA-derived BV equation is estimated utilising the density coefficients of lean mass (LM), FM and BMC. This could potentially introduce error since recent research has shown that the FFM density (DFFM = 1·105 and 1·107 g/cm3), TBWFFM (72·00 and 71·88 %) and MoFFM (4·95 and 5·51 %) of Hispanic males and females, respectively, differ from the aforementioned ‘reference body’ of white males (1·100 g/cm3, 73·8 and 5·6 %, respectively)(Reference Nickerson, Tinsley and Fedewa10). For these reasons, Hispanic FFM characteristics may not be comparable with the LM, FM and BMC densities obtained in validation samples for each DXA-derived BV equation, which consisted primarily of non-Hispanic whites. Therefore, the purpose of the present study was (1) to develop a new DXA-derived BV equation with the GE-Lunar prodigy (DXANickerson) while utilising UWW as a criterion and (2) to cross-validate 4C-DXANickerson, 4C-DXAWilson(Reference Wilson, Strauss and Fan17) and 4C-ADP in Hispanic adults.
Experimental methods
Participants
A sample of 191 Hispanic adults participated in the present study. Participant characteristics are displayed in Table 1. A sample of 120 participants (50 % females) was randomly selected to develop the coefficients reported in Equation 1. The remaining sample of seventy-one participants (females: 41; males: 30) was utilised as a cross-validation sample. Eligible participants were (1) adults between 18 and 45 years of age, (2) self-reported Hispanic ethnicity, (3) were apparently healthy with no self-reported medical conditions that would impact body composition assessments (e.g. dialysis treatment) and (4) <350 lbs (159 kg) due to DXA table restrictions. Pregnant women and those who were pregnant within the previous 12 months were also excluded from the present study. All body composition assessment measurements were performed on the same day during a single visit that lasted approximately 90–120 min. Participants were asked to avoid eating or drinking 12 h prior to participation and to also avoid exercise 24 h before testing. Testing times occurred in the morning from 06.00 to 10.00 hours. Prior to testing, participants provided written informed consent and completed a self-reported medical history questionnaire. The present study was approved by the Institutional Review Board of the host university.
Subject characteristics for development and cross-validation sample
(Mean values and standard deviations; minimum and maximum values)
4C, four-compartment model; UWW, underwater weighing; FFM, fat-free mass; Db, body density; ADP, air displacement plethysmography; TBW, total body water; BMC, bone mineral content; BV, body volume; DXA, dual-energy X-ray absorptiometry.
Procedures
Upon completion of the informed consent and medical history questionnaire, participants’ hydration status was assessed from a urine sample using a handheld refractometer (Atago SUR-NE; Atago Corp. Ltd). Urine specific gravity values <1·029 were required for participation(Reference Armstrong26). After confirming adequate hydration, body mass was measured (to the nearest 0·1 kg) with a calibrated digital weighing scale (Tanita BWB-800; Tanita Corporation), whereas height was measured to the nearest 0·1 cm with a stadiometer (SECA 213; Seca Ltd).
Dual-energy X-ray absorptiometry
DXA (2016 GE-Lunar Prodigy, enCORE v17; GE Lunar Corporation) was used to measure LM, FM and BMC in order to develop the coefficients for the new DXA-derived BV equation and to estimate BV via the Wilson(Reference Wilson, Strauss and Fan17) equation. Prior to each whole-body scan, DXA was calibrated according to the manufacturer’s instructions via a standard calibration block. Participants removed shoes, socks and all jewellery and were instructed to lie supine on the scanning bed with hands by the sides. During all body scans, participants were asked to remain motionless, while Velcro straps were situated around the ankles and knees. Scans lasted approximately 6–10 min. The same researcher positioned all participants on the DXA scanning bed. In addition, the trained researcher analysed each scan to adjust software-determined regions of interest prior to producing the whole-body report. Finally, the prediction equation (Eq. 2) proposed by Wilson(Reference Wilson, Strauss and Fan17) was used to estimate BV for 4C-DXAWilson. The symbol V represents the volumetric densities of fat, lean soft tissue and BMC, whereas the coefficient V Residual represents the residual volume that the three DXA mass compartments do not fully capture in the estimate of total BV.
$${\rm{DXA \ volume }}\,\left( {\rm{litres}} \right)\, = \,\left( {{\rm{FM}}/{V_{{\rm{Fat}}}}} \right)\, + \,\left( {{\rm{LM}}/{V_{{\rm{Lean}}}}} \right)\, + \,\left( {{\rm{BMC}}/{V_{{\rm{BMC}}}}} \right)\, + \,{V_{{\rm{Residual}}}}$$Wilson equation(Reference Wilson, Strauss and Fan17):
$${\rm{DXA \ volume }}\,\left( {\rm{litres}} \right)\, = \,\left( {{\rm{FM}}/0\!\cdot\!87} \right)\, + \,\left( {{\rm{LM}}/1\!\cdot\!072} \right)\,-\,\left( {{\rm{BMC}}/2\!\cdot\!283} \right)\, + \,1\!\cdot\!504$$DXA was also used to calculate BMC for all 4C models. The total body BMC was converted to total body bone mineral (Mo). Mo was calculated as follows: Mo = total body BMC (kg) × 1·0436(Reference Heymsfield, Wang and Heshka27).
Bioimpedance spectroscopy
Bioimpedance spectroscopy was used to determine TBW (ImpTM SFB7; ImpediMed Limited) for all of the 4C models evaluated in the present study. Bioimpedance spectroscopy testing occurred immediately after DXA scans since assessments for the bioimpedance spectroscopy device require participants to lay a minimum of 5 min prior to measurements. The velcro straps were removed following the DXA scan to allow for proper placement during the bioelectrical impedance analysis (BIA) measurement. Also following the DXA scan, excess hair was removed and the skin was cleaned with alcohol pads and dried prior to the BIA measurements at each of the electrode sites to ensure proper conduction of the electrical currents. The participants’ right shoe and sock remained off, and their arms placed ≥30° away from the body with legs separated and not touching. Two single tab electrodes were placed at the distal end of the participant’s (1) right wrist and hand and (2) right ankle and foot, with 5 cm between each set of electrodes in order to measure TBW.
Air displacement plethysmography
The BOD POD® (COSMED USA Inc.) was used to estimate BV for 4C-ADP in the cross-validation sample. Prior to each testing day, the BOD POD® was calibrated according to manufacturer specifications. Participants were required to wear minimal, lycra compression clothing, and lycra swim-caps were provided and required for testing. Body mass was measured using the manufacturer’s scale that interfaces with the BOD POD®. In order to assess BV, participants were instructed to sit in the BOD POD® chamber for two trials of roughly 50 s for each trial. A third trial was necessary if the first two trials did not agree within 150 ml of each other. Thoracic gas volume was estimated for all assessments.
Underwater weighing
UWW was performed in an apparatus specifically designed for densitometry measurements (Vacu-Med). The BV measurement obtained in the development sample was used as the criterion variable in order to develop the new DXA-derived BV equation. In addition, UWW-derived BV was used to calculate 4C-UWW BF % and FFM in the cross-validation sample. All UWW tests were performed last due to the impact that a warm water temperature could have on body temperature. Water temperature for testing was approximately 35–38°C for all participants. Furthermore, each UWW test lasted approximately 10–15 min for participants. Prior to the UWW measurements, participants changed into compression type clothing or a bathing suit. Participants sat on a sling seat during testing. The participants were instructed to perform a maximum expiration and submerge completely underwater. Prior to all UWW testing, residual lung volume was completed using a nitrogen analyser on dry land in a seated position (Vacu-Med).
Four-compartment model calculation
The 4C model is based upon the equation described by Wang et al.(Reference Wang, Shen, Withers, Heymsfield, Lohman and Wang28) and is described as follows:
$$\matrix{\scale 98%{{\rm {FM}} \,\left( {\rm{kg}} \right) = 2\!\cdot\!748\left( {{\rm{BV}}} \right)-0\!\cdot\!699\left( {{\rm{TBW}}} \right) + 1\!\cdot\!129\left( {{\rm{Mo}}} \right)-2\!\cdot\!051\left( {{\rm{BM}}} \right)} \hfill \cr \scale 95%{{\rm{FFM}}\,\left( {{\rm{kg}}} \right) = \left( {{\rm{BM}} - {\rm{FM}}} \right)} \hfill \cr \scale 95%{{\rm{BF}}\,{\rm{\% }} = \left( {{\rm{FM}}/{\rm{BM}}} \right) \times 100} \hfill \cr } $$Statistical analyses
Multivariate linear regression using the enter method was used to derive the new DXA-derived BV equation in the development sample. Data were screened for outliers and normal distribution with skewness or kurtosis >2 indicating non-normal distribution(Reference Cohen, Cohen and West29). Distributions of all independent variables from the DXA scan (FM, LM and BMC) and variables used in the 4C model (BMC, TBW, UWW-derived BV, 4C-ADP, 4C-DXAWilson, 4C-DXANickerson and 4C-UWW) were normal. The BV from UWW was used as the dependent variable, and FM, LM and BMC from DXA were used as independent factors in order to derive the coefficients in Eq. (1). The new coefficients were subsequently utilised to calculate DXA-derived BV in the cross-validation sample in order to determine the accuracy of the new equation in a 4C model for the estimation of BF % and FFM in Hispanic adults.
The validity of 4C-ADP, 4C-DXAWilson and 4C-DXANickerson was based upon comparisons with the 4C-UWW by calculating the constant error, r value, standard error of estimate, total error (TE) and proportional bias(Reference Heyward and Wagner30,Reference Tinsley31) . The mean differences in BF % and FFM (i.e. constant error) among the 4C models were compared with the 4C-UWW (e.g. 4C-DXANickerson – 4C-UWW) and analysed using dependent t tests (SPSS version 24) with the Bonferroni-adjusted α level (P ≤ 0·0166). Differences between the UWW criterion, ADP and DXA measures were assessed using a standardised mean effect size (ES), by dividing the difference between the criterion and alternative measure by the standard deviation of the criterion. Threshold values for the standardised ES were 0·2, 0·5 and 0·8 for small, moderate and large differences, respectively(Reference Lipsey and Wilson32). The following thresholds were used to describe the r values: 0–0·30 small, 0·31–0·49 moderate, 0·50–0·69 large, 0·70–0·89 very large and 0·90–1·00 near-perfect(Reference Hopkins, Marshall and Batterham33). The method of Bland–Altman was used to identify the 95 % limits of agreement (LOA) of the 4C models(Reference Bland and Altman34). An a priori power analysis was performed using G*power (version 3.1.9.3) and indicated that thirty-four participants were needed in order to detect a moderate difference between two body composition assessment methods (power = 0·80, α level = 0·05)(Reference Faul, Erdfelder and Lang35,Reference Faul, Erdfelder and Buchner36) .
Results
Participants were aged 18–45 years (25·74 (sd 7·15) years) and mostly female (53·1 %). Standing height ranged from 147·00 to 193·60 cm (166·76 (sd 8·98) cm), and weight ranged from 36·30 to 136·30 kg (73·01 (sd 17·12) kg). Finally, BMI ranged from 16·46 to 42·34 kg/m2 (26·07 (sd 4·84) kg/m2), with 37·69 and 18·23 % of the sample classified as overweight and obesity, respectively. Additional participant characteristics for the development and cross-validation samples are stratified by sex and presented in Table 1.
Body volume equation
The inverse of the density coefficients of FM, LM and BMC determined for the new DXA-derived BV equation was 0·91 (P < 0·001), 1·06 (P < 0·001) and 16·95 (P = 0·841), respectively, with a residual volume of 0·268 litres.
$${\rm{DX}}{{\rm{A}}_{{\rm{Nickerson}}}}\,{\rm{volume }}\,\left( {\rm{litres}} \right)\, = \,\left( {{\rm{FM}}/0\!\cdot\!91} \right)\, + \,\left( {{\rm{LM}}/1\!\cdot\!06} \right)\, + \,\left( {{\rm{BMC}}/16\!\cdot\!95} \right)\, + \,0\!\cdot\!268 $$Body fat percentage
The validity of 4C-DXAWilson, 4C-DXANickerson and 4C-ADP when compared with 4C-UWW is displayed in Table 2. Small non-significant differences were observed in the cross-validation sample of females (ES = –0·12, P = 0·06) and males (ES = –0·04, P = 0·61), when evaluating the 4C-DXANickerson. Similarly, small non-significant differences were observed with the 4C-ADP in the cross-validation sample of females (ES = –0·08, P = 0·12) and males (ES = –0·11, P = 0·05). Contrarily, 4C-DXAWilson yielded a moderate difference when compared with 4C-UWW in females (ES = 0·48, P < 0·001), but not in males (ES = 0·15, P = 0·06). Near-perfect correlation coefficients were observed for all methods when evaluated in both sexes, as observed r values ranged from 0·903 to 0·961. The TE was lowest for 4C-ADP in both sexes and was fairly similar between 4C-DXAWilson and 4C-DXANickerson in males, whereas the 4C-DXANickerson equation performed better in females. No proportional bias was observed in males for any of the comparisons, as the correlations ranged from –0·03 to 0·13 (all P > 0·05). However, 4C-DXANickerson was the only prediction method that did not produce significant proportional bias when evaluated in females (r 0·11, P = 0·071). The significant proportional bias coefficients observed for 4C-ADP (r 0·19) and 4C-DXAWilson (r 0·23) in females indicate that BF % is overestimated at higher 4C-UWW adiposity levels (both P < 0·001). Fig. 1 displays the Bland–Altman plots (i.e. 95 % LOA) and proportional bias (i.e. trend line) for all BF % measurements.
Validation of dual-energy X-ray absorptiometry (DXA)- and air displacement plethysmography (ADP)-derived body volume (BV) in cross-validation sample of females (n 41) and males (n 30) when using a four-compartment (4C) model
(Mean values and standard deviations)
BF %, body fat percentage; UWW, underwater weighing; FFM, fat-free mass.
Bland–Altman plot for body fat percentage (BF %) in Hispanic females and males. The middle solid line represents the constant error (CE) between the four-compartment (4C) prediction models (4C-DXAWilson, 4C-DXANickerson and 4C-ADP) and the criterion 4C-underwater weighing (UWW). The two outside dashed lines indicate the 95 % CI of the bias (difference) and their means. The dashed–dotted line represents the linear regression fit line. Separate Bland–Altman plots depicting BF % in the 4C prediction models are displayed for (a) 4C-DXAWilson in females; (b) 4C-DXANickerson in females; (c) 4C-ADP in females; (d) 4C-DXAWilson in males; (e) 4C-DXANickerson in males and (f) 4C-ADP in males. DXA, dual-energy X-ray absorptiometry; ADP, air displacement plethysmography.
Fat-free mass
The mean values were not statistically significant in the cross-validation sample of females (ES = 0·05, P = 0·174) and males (ES = 0·01, P = 0·812), when evaluating the 4C-DXANickerson. Similarly, 4C-ADP yielded small mean differences when evaluated in the cross-validation sample of females (ES = 0·02, P = 0·420) and males (ES = 0·05, P = 0·090). Contrarily, 4C-DXAWilson yielded a moderate difference compared with 4C-UWW when evaluated in females (ES = –0·30, P < 0·001), but not in males (ES = –0·09, P < 0·058). Near-perfect correlation coefficients were observed for all methods when evaluated in both sexes, as observed r values ranged from 0·942 to 0·990. The TE was lowest for 4C-ADP in both sexes and was fairly similar between 4C-DXAWilson and 4C-DXANickerson in males. In contrast, the 4C-DXANickerson performed better than the 4C-DXAWilson in females. The 4C-ADP was the only method that produced significant proportional bias when evaluating FFM. Specifically, a negative coefficient was observed in females, which indicates that FFM is underestimated at higher levels. Fig. 2 displays the Bland–Altman plots (i.e. 95 % LOA) and proportional bias (i.e. trend line) for all FFM measurements.
Bland–Altman plot for fat-free mass (FFM) in Hispanic females and males. The middle solid line represents the constant error (CE) between the four-compartment (4C) prediction models (4C-DXAWilson, 4C-DXANickerson and 4C-ADP) and the criterion 4C-underwater weighing (UWW). The two outside dashed lines indicate the 95 % CI of the bias (difference) and their means. The dashed–dotted line represents the linear regression fit line. Separate Bland–Altman plots depicting FFM in the 4C prediction models are displayed for (a) 4C-DXAWilson in females; (b) 4C-DXANickerson in females; (c) 4C-ADP in females; (d) 4C-DXAWilson in males; (e) 4C-DXANickerson in males; (f) 4C-ADP in males. DXA, dual-energy X-ray absorptiometry; ADP, air displacement plethysmography.
Discussion
All three methods (i.e. 4C-DXAWilson, 4C-DXANickerson and 4C-ADP) appear to be interchangeable with 4C-UWW when used in a group of apparently healthy Hispanic males. Nonetheless, due to the lower constant error and TE values, 4C-DXANickerson is recommended over the 4C-DXAWilson in Hispanic males when 4C-ADP and 4C-UWW are not practical or available. In addition, the 4C-DXANickerson performed better than the 4C-DXAWilson when evaluating BF % and FFM in Hispanic females. Lastly, these findings demonstrate that sex and level of fatness may impact the accuracy of 4C-ADP and 4C-DXAWilson, but that 4C-DXANickerson is able to overcome these issues.
The present study results advance previous work. For instance, Nickerson et al.(Reference Nickerson, Esco and Bishop37) found larger standard error of estimates (3·9–4·2 %) and 95 % LOA (±7·9–8·2 %) than the present study when estimating 4C model BF % with the DXA-derived BV equation from Smith-Ryan et al.(Reference Smith-Ryan, Mock and Ryan16) in a group of physically active men and women. In contrast, Blue et al.(Reference Blue, Hirsch and Trexler38) evaluated a group of overweight and obese individuals and revealed that the DXA-derived BV equation from Smith-Ryan et al.(Reference Smith-Ryan, Mock and Ryan16) produced similar TE values (2·92 %) as many of the comparisons observed in the present study. The reason for the differences between Nickerson et al.(Reference Nickerson, Esco and Bishop37) and Blue et al.(Reference Blue, Hirsch and Trexler38) could be related to the criterion measurements utilised to determine BV in the criterion 4C model. For instance, Nickerson et al.(Reference Nickerson, Esco and Bishop37) incorporated UWW-derived BV in their criterion 4C model, whereas Blue et al.(Reference Blue, Hirsch and Trexler38) and Smith-Ryan et al.(Reference Smith-Ryan, Mock and Ryan16) used ADP as a criterion when assessing the validity of their DXA-derived BV equation. Due to these differences, it is not surprising to see the DXA-derived BV equation perform better for Blue et al.(Reference Blue, Hirsch and Trexler38) than Nickerson et al.(Reference Nickerson, Esco and Bishop37). Furthermore, the observed differences in the relative accuracy of the DXA-derived BV equations may be due to the study populations in which they were developed when considering the (1) population differences in FM, FFM and bone mineral density in blacks, Hispanics and whites(Reference Berenson, Breitkopf and Newman39,Reference Alemán-Mateo, Lee and Javed40) , (2) coupled with the age-related increase in FM, decrease in total FFM and decrease in the relative contribution of BMC as a fraction of FFM(Reference Snead, Birge and Kohrt41,Reference Guo, Zeller and Chumlea42) and (3) changes in FFM characteristics for physically active individuals(Reference Collins, Millard-Stafford and Evans43,Reference Nickerson and Tinsley44) .
McLester et al.(Reference McLester, Nickerson and Kliszczewicz20) advanced these findings and found that the Hologic DXA-derived BV equation from Smith-Ryan et al.(Reference Smith-Ryan, Mock and Ryan16) produced larger 95 % LOA than the Wilson et al.(Reference Wilson, Fan and Shepherd18) equation when used in a 4C model for individuals with varying BMI and waist circumference values. Further, both Hologic DXA-derived BV equations produced proportional bias for many comparisons(Reference Smith-Ryan, Mock and Ryan16,Reference Wilson, Fan and Shepherd18) . The findings observed by McLester et al.(Reference McLester, Nickerson and Kliszczewicz20) are similar to the present study, which revealed that the GE-Lunar Prodigy DXA-derived BV equation from Wilson et al.(Reference Wilson, Strauss and Fan17) produced significant proportional bias when evaluated in the cross-validation sample of Hispanic females. There appears to be little physiological reason why the Wilson equation would yield such poor accuracy solely because of sex. Given that BF % among female participants in the present study was roughly 10 % higher than among male participants, it is more likely that the observed bias is due to the sex-related differences in BF % and poor accuracy in obese individuals. Nonetheless, the new DXA-derived BV equation was able to overcome these limitations and produced small constant errors, standard error of estimates, TE and 95 % LOA in addition to the absence of proportional bias. Whether the superior performance is due to biological sex or body composition, allied health professionals are encouraged to adopt the new DXA-derived BV equation for Hispanic adults when assessing body composition with a modified 4C model (i.e. DXA-derived BV and BMC; bioimpedance spectroscopy-derived TBW).
This is the first study to utilise three different laboratory-based BV measures in a multi-compartment model (i.e. UWW, ADP and DXA) at the same time point. Nickerson et al. (Reference Nickerson, Tinsley and Esco21) compared various modified 3C models using skinfold- (i.e. Db/body mass), DXA- and UWW-derived BV and revealed that the aforementioned methods, when used in a multi-compartment model, improve upon stand-alone DXA body composition assessments. The findings from Nickerson et al. (Reference Nickerson, Tinsley and Esco21) demonstrate the importance of accounting for TBW in a multi-compartment model, which is unaccounted for in a stand-alone DXA body composition output. Nonetheless, prior to our findings, previous DXA-derived BV research could only be generalised to a single criterion BV reference being utilised for analysis (i.e. ADP or UWW). The present study utilised UWW instead of ADP as a reference in the 4C model since it has traditionally been regarded as the ‘gold standard’ for BV assessments and has yet to be utilised for the derivation of a DXA-derived BV equation(Reference Brozek, Grande and Anderson9,Reference Siri22,Reference Heymsfield23) . The authors believe that the strength of the present study is bolstered by the use of the UWW criterion for measuring BV. However, it is unclear how the choice of a criterion measure (UWW v. ADP) impacted the development of the DXANickerson equation. Given that the size of the observed differences between the BF % measures comparing the 4C-DXANickerson and 4C-ADP estimates of BF % and FFM appeared to be negligible, it is doubtful that the use of an ADP criterion to derive each of the density coefficients would have drastically changed the results. Determining the impact of criterion choice (UWW v. ADP) on subsequent density coefficients when developing a novel DXA volume equation, and the subsequent accuracy of each equation in cross-validation studies, provides an interesting opportunity for future research but was beyond the scope of the present study.
Uniquely, the present study demonstrates that the GE-Lunar Prodigy DXA-derived BV equation from Wilson et al.(Reference Wilson, Strauss and Fan17) produces similar proportional bias as ADP-derived BV in a 4C model when evaluated in Hispanic males and females. Contrarily, the new DXA-derived BV equation was unaffected and did not produce proportional bias for any comparisons. The reason for the difference could be attributed to a number of different factors worth discussion. For instance, the GE-Lunar Prodigy DXA-derived BV equation from Wilson et al.(Reference Wilson, Strauss and Fan17) was developed using ADP as a criterion measure. In addition, Wilson et al.(Reference Wilson, Strauss and Fan17) utilised a retrospective analysis of clinical patients aged 10–89 years with chronic renal disease, intestinal failure, anorexia, malnutrition, obesity, liver disease, metabolic disorders and others, whereas the present study sample consisted of apparently healthy Hispanic adults 18–45 years of age. It is commonly understood that conditions such as anorexia(Reference Fazeli45), malnutrition(Reference Kueper, Beyth and Liebergall46,Reference Siddique, O’Donoghue and Casey47) and metabolic disorders(Reference Wong, Chin and Suhaimi48) negatively impact bone health. As such, equations derived from a sample of clinical patients may be inaccurate when applied to the general population. In addition, the ethnicity of participants for the Wilson et al.(Reference Wilson, Strauss and Fan17) study was not disclosed. However, given the geographical location of the study site (i.e. Melbourne and Australia) for Wilson et al.(Reference Wilson, Strauss and Fan17), it is logical that the group of Hispanics in the present study differed greatly from the former study population.
The LM coefficient for the new DXA-derived BV equation is fairly similar to the Wilson et al.(Reference Wilson, Strauss and Fan17) equation. However, there are noticeable differences in the intercept as well as the FM and BMC coefficients. In regard to the latter, BMC was a significant predictor (P < 0·001) of DXA-derived BV for Wilson et al.(Reference Wilson, Strauss and Fan17), which differs from the present study findings, which revealed that BMC was not statistically significant (P = 0·841). The present study findings are similar to results obtained by Smith-Ryan et al.(Reference Smith-Ryan, Mock and Ryan16) who also found that BMC was not statistically significant (P = 0·853) when developing a DXA-derived BV equation with a Hologic DXA scanner. The reason for similarities between the present study and Smith-Ryan et al.(Reference Smith-Ryan, Mock and Ryan16) is likely because both populations were apparently healthy. BMC has minimal variation in apparently healthy populations. Contrarily, there can be large BMC differences in diseased and older populations(Reference Nickerson and Tinsley44) such as those included in the Wilson et al.(Reference Wilson, Strauss and Fan17) study sample. Interestingly, MoFFM was similar for all groups (i.e. 4·7–5·2 %) in the present study but varied from the assumed values employed in 2C models (i.e. 5·6 %), which are based upon analysis of non-Hispanic white males. Therefore, the small, but consistent, amount of FFM that is comprised of BMC in our Hispanic population appears to have minimal benefit when used for the estimation of BV. Thus, further research utilising populations with varying BMC is warranted.
Additional considerations for the present study findings are in need of further discussion. For instance, Wilson et al.(Reference Wilson, Strauss and Fan17) noted that all DXA scans were made using the medium-thickness scan mode. This could have introduced bias when scanning thin or overweight/obese participants in the present study. For instance, the DXA model used in the present study has three different scan modes (i.e. thin, standard and thick), which is automatically selected by the software based upon each subject’s physical characteristics. Therefore, the use of a standard scan mode for all participants is not appropriate. For example, a standard scan mode for participants with excessive adiposity, especially some of those in the present study who had BMI values classified as obese (BMI ≥ 30 kg/m2), would likely have produced different regression coefficients. This might be why there was proportional bias for 4C-DXAWilson when assessing the Hispanic female participants with higher BF % values, which further highlights that the level of fatness may have impacted the validity of the Wilson et al.(Reference Wilson, Strauss and Fan17) DXA-derived BV equation.
While this study was carefully designed and executed, it is not without limitations. First, a sample of apparently healthy Hispanic adults limited to the ages of 18–45 years served as the development population. Therefore, results cannot be applied to older Hispanic adults or those with significant health issues. Similarly, it is unknown whether the new DXA-derived BV equation can be generalised in ethnicities that differ from our Hispanic sample. Accordingly, further validation research is warranted to determine whether the 4C-DXANickerson can be applied to populations that differ from the present study sample. It should also be noted that the volume coefficients were developed using measurements from a GE Prodigy model. Aside from the age, sex, race and training status, the specific DXA model should also be considered when examining these results in the context of the larger body of research. This equation should be extensively validated in other DXA models in female and male participants, representing various racial–ethnic groups across the lifespan. Lastly, the present study sought to use only data from Hispanic adults to develop and validate the new DXA-derived BV equation. Ethnicity in the present study was self-reported, and it is unclear how much potential error was introduced by a participant identifying as Hispanic ethnicity despite no Hispanic/Latino ancestry. The authors are unaware of any such instances in the present study, but the possibility of an inaccurate or misleading participant response still exists.
In conclusion, all three methods are valid for the estimation of BF % and FFM in apparently healthy Hispanic males. 4C-ADP produced the lowest TE values, while 4C-DXANickerson produced slightly better coefficients than 4C-DXAWilson. These findings highlight that DXA can be used as a surrogate of ADP in a 4C model for Hispanic males. However, 4C-DXANickerson was the best method when evaluating BF % and FFM in the group of Hispanic females due to the bias observed in 4C-ADP and 4C-DXAWilson. For instance, 4C-DXAWilson produced significant mean differences for BF % and FFM and had significant proportional bias when estimating FFM, whereas 4C-ADP revealed significant proportional bias for both BF % and FFM when evaluated in Hispanic females. Collectively, the present study findings demonstrate that 4C-DXANickerson is a valid measure in Hispanic females and males and is recommended for use in clinics, where DXA is the main body composition assessment technique.
Acknowledgements
The authors would like to acknowledge Gabriel Perez, Andres Orozco, Isabel Delgado, Samantha Narvaez, Misael Duque and Joshua Carrillo for their efforts in the data collection of the present study.
Equipment utilised in the present study was financially supported by Texas A&M International University (start-up funds).
B. S. N. designed the study and collected the data for analysis. All authors interpreted the data. B. S. N. wrote the manuscript with input from M. V. F., C. N. M., J. R. M. and M. R. E. All authors approved the final version of the manuscript.
The authors declare that there are no conflicts of interest.
Supplementary material
For supplementary material referred to in this article, please visit https://doi.org/10.1017/S0007114520000598
