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The metabolism of some folates in the rat

Published online by Cambridge University Press:  25 March 2008

J. R. G. Beavon  and
J. A. Blair
J. R. G. Beavon
Affiliation:
Department of Chemistry, The University of Aston in Birmingham, Gosta Green, Birmingham B4 7ET
J. A. Blair
Affiliation:
Department of Chemistry, The University of Aston in Birmingham, Gosta Green, Birmingham B4 7ET
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Abstract

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1. A number of folates labelled with 14C were administered orally to rats, at various doses, and urinary, faecal and hepatic folates examined.

2. 10-Formylpteroylmonoglutamic acid (10CHO—PGA) entered the folate pool very slowly, and is thought to be relatively ineffective in nutrition.

3. 10-Formyl[2-14C]tetrahydrofolic acid (10CHO—[2-14C]THF) entered the folate pool very rapidly. 5-Methyl[2-14C]tetrahydrofolate (5CH3—[2-14C]THF) was the major urinary folate.

4. 5-Formyl[2-14C]tetrahydrofolic acid (5CHO—[2-14C]THF) entered the folate pool only to a small extent. 5CHO—[2-14C]THF, given intravenously, produced no urinary 5CH3—[2-14C]THF in the first 6 h.

5. 10-Methylidyne[2-14C]tetrahydrofolic acid was metabolized to an extent which was dependant on the dose. At doses of 3 and 30 μg/kg body-weight, 5CH3—[2-14C]THF represented 5·4 and 20% respectively of urinary folates and for 10CHO—[2-14]PGA, the values were 16% of total urinary folates after the higher dose, and 78·5% after the lower dose.

6. Results obtained for the metabolism of 5CH3—THF varied depending on the position of the labelling: 514CH3–THF gave no labelled urinary folate, the methyl group being lost rapidly. When 5CH3—[2-14C]THF was given, it appeared as the major urinary folate.

7. Folates found in the liver after oral administration of labelled folates were identified by thin-layer chromatography; only folate monoglutamates were identified.

Information

Type
General Nutrition
Information
British Journal of Nutrition , Volume 33 , Issue 3 , May 1975 , pp. 299 - 308
Copyright
Copyright © The Nutrition Society 1975

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