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Effect of high fat diet and moderate exercise on T-lymphocytes of Peyer's patches mice

Published online by Cambridge University Press:  04 August 2011

M. G. Zúñiga-Torres ,
B. E. Martínez-Carrillo ,
R. A. Jarillo-Luna ,
V. Rivera-Aguilar ,
R. Campos-Rodriguez ,
R. V. Pardo-Morales ,
C. Rosales-Gómez  and
R. Valdés-Ramos
M. G. Zúñiga-Torres
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México
B. E. Martínez-Carrillo
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico
R. A. Jarillo-Luna
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico
V. Rivera-Aguilar
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico
R. Campos-Rodriguez
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico
R. V. Pardo-Morales
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México
C. Rosales-Gómez
Affiliation:
Laboratorio de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico
R. Valdés-Ramos
Affiliation:
Center for Research and Graduate Studies in Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México
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Abstract

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Moderate physical exercise alone has a positive influence on the immune system(Reference Pedersen and Hoffman-Goetz1) but the effect of a high fat diet (↑LIPIDS)(Reference Kaminogawa and Nanno2) combined with moderate physical exercise in young people has not been established; the objective of this study was to assess whether a high fat diet and moderate physical exercise modifies the T-lymphocyte (TLc) population of Peyer's patches (PP) of young Balb/c mice. Thirty-two 21-d-old male mice were divided into four groups, two groups swam for 30 min, 5 d per week during 9 weeks (E groups) and two groups were left without exercise; one of the exercise and one without exercise were fed a standard diet (CONTROL) and the other two groups were fed a high fat diet (↑LIPIDS) (DIO Rodent Purified Diet, Cat. 58V8; energy: 3.78 kcal/g (15.81552 kJ/d)). Small intestines were dissected, PP were cut, macerated, filtered and centrifuged to obtain the lymphocyte pellet, cells were stained with anti-CD3+, CD4+ and CD8+ antibodies. The diet only increased the weight of mice who made exercise (25.7, sd 0.0) compared with non-exercise group (21.5, sd 2.8) and the control group (20.2, sd 1.4), P<0.005. CD3+ TLc (25.6, sd 1.0), CD3+/CD4+ (20.8, sd 1.3) and CD3+/CD8+ (2.62, sd 0.3) decreased significantly in the group ↑LIPIDS/E (in comparison with CONTROL/E: 38.8, sd 2.5, t 13.87; 33.5, sd 2.0, t 15.01; 4.93, sd 0.5, t 10.96, respectively), P⩽0.001. Thus, the diet did not change the weight of the animals but significantly altered the amount of T-cells, this probably due to the high content of saturated fat in the diet (2.91% from soya oil; 20.69% from lard) (Fig. 1).

Fig. 1.

Percentage of lymphocytes CD3+, CD3+/CD4+ and CD3+/CD8+ from PP of Balb/c mice, feeding with standard diet (CONTROL) or high fat diet (↑LIPIDS); with 30 min exercise (/E), or without it. Values are show in means (sd) (n 8), differences were statistically significant by Student's t test at P⩽0.001. CONTROL: group with standard diet without exercise; ↑LIPIDS: group with high fat diet; CONTROL/E: group with standard diet with exercise; ↑LIPIDS/E: group feeding with high fat diet and with exercise.

This project was financed by Universidad Autónoma del Estado de México.

References

1.Pedersen, BK & Hoffman-Goetz, L (2000) Physiol Rev 80, 1055–1081.CrossRefGoogle Scholar
2.Kaminogawa, S & Nanno, M (2004) Altern Med 1, 241–250.Google Scholar
Figure 0

Fig. 1. Percentage of lymphocytes CD3+, CD3+/CD4+ and CD3+/CD8+ from PP of Balb/c mice, feeding with standard diet (CONTROL) or high fat diet (↑LIPIDS); with 30 min exercise (/E), or without it. Values are show in means (sd) (n 8), differences were statistically significant by Student's t test at P⩽0.001. CONTROL: group with standard diet without exercise; ↑LIPIDS: group with high fat diet; CONTROL/E: group with standard diet with exercise; ↑LIPIDS/E: group feeding with high fat diet and with exercise.