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The effect of molybdenum on the conversion of sulphate to sulphide and microbial-protein-sulphur in the rumen of sheep

Published online by Cambridge University Press:  25 March 2008

J. M. Gawthorne  and
C. J. Nader
J. M. Gawthorne
Affiliation:
CSIRO, Division of Nutritional Biochemistry, Kintore Avenue, Adelaide, South Australia 5000, Australia
C. J. Nader
Affiliation:
CSIRO, Division of Nutritional Biochemistry, Kintore Avenue, Adelaide, South Australia 5000, Australia
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Abstract

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1. [35S]sulphate was used to measure the apparent turnover of sulphate, sulphide and microbial-protein-S in the rumen contents of four sheep that were intraruminally infused with 10 g sodium sulphate/d alone, or together with 126 mg sodium molybdate (50 mg molybdenum/d).

2. Infusion of molybdate increased the concentration of sulphate in rumen fluid from 2.2 to 7.2 μg S/ml and decreased the rate of reduction of sulphate to sulphide by 50%. Although the rate of sulphide production was slower, the concentration of sulphide in rumen contents was increased. A dual role for molybdate in the metabolism of sulphide in the rumen is suggested to explain these changes.

3. In animals that were not infused with molybdate, only one-third of the S (3.0 g/d) that passed through the sulphate pool in rumen fluid was converted to sulphide, decreasing to one-sixth when molybdate was infused.

4. The turnover of S amino acids in microbial protein was not significantly affected by molybdate. Only 52–57% of the S amino acid content of microbial protein was synthesized de novo by way of the sulphide pool.

Information

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 35 , Issue 1 , January 1976 , pp. 11 - 23
Copyright
Copyright © The Nutrition Society 1976

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