Sperm Cryopreservation

David Mortimer; Lars Björndahl; Christopher L. R. Barratt; José Antonio Castilla; Roelof Menkveld; Ulrik Kvist; Juan G. Alvarez; Trine B. Haugen

doi:10.1017/9781009181648.010

Chapter 10 - Sperm Cryopreservation

Published online by Cambridge University Press: 16 February 2022

David Mortimer ,

Lars Björndahl ,

Christopher L. R. Barratt ,

José Antonio Castilla ,

Juan G. Alvarez and

David Mortimer: Affiliation:
Oozoa Biomedical Inc., Vancouver
Lars Björndahl: Affiliation:
Karolinska Institutet, Stockholm
Christopher L. R. Barratt: Affiliation:
University of Dundee
José Antonio Castilla: Affiliation:
HU Virgen de las Nieves, Granada
Roelof Menkveld: Affiliation:
Stellenbosch University, South Africa
Ulrik Kvist: Affiliation:
Karolinska Institutet, Stockholm
Juan G. Alvarez: Affiliation:
Centro ANDROGEN, La Coruña
Trine B. Haugen: Affiliation:
Oslo Metropolitan University

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Summary

The basic princples of cryobiology are described for both slow freezing and vitrification of spermatozoa. Specific aspects of cryopreserving human spermatozoa are discussed in detail, incluidng the formulation of cryopreservation media and their proper use. Alternative packaging devices are discussed in relation to the achievement of correct cooling and warming curves as well as effective biocontainment. High security straws are recommended as the best method to use from both perspectives, and a standard operating procedure (SOP) for easy use at the bench is provided. SOPs for human sperm vitrification techniques are also gven. Quality control and risk management aspects of sperm freezing and for cryobank organzation are described. Finally, there is a section on sperm donation.

Keywords

sperm freezing cryopreservaton vitrification cryobanking quality management risk management sperm donation sperm donor

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Type: Chapter
Information: A Practical Guide to Basic Laboratory Andrology , pp. 218 - 255

DOI: https://doi.org/10.1017/9781009181648.010 [Opens in a new window]

Publisher: Cambridge University Press

Print publication year: 2022

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