The principles for safety assessment of GM crops are reflected in the internationally binding Cartagena Protocol on Biosafety. However, only in countries with a functional biosafety regulatory system will people be able to take full advantage of the bioeconomy. Based on experience and reviews of biosafety capacity building and training initiatives, some best practices are identified. Generally, countries with existing capacity for biotechnology R&D, and that have political support, have functioning regulatory systems and have benefited most from technical assistance programmes. The majority of developing countries are not yet able to implement their biosafety frameworks. Agencies implementing training initiatives need to balance them with commitment from governments. National frameworks can be tailored effectively for large, agricultural producing countries on one hand, and countries that primarily import agricultural commodities on the other. Regional collaboration would enable countries to share expertise and safety assessments. This chapter proposes that biosafety training should be linked to hands-on decision making to improve regulatory implementation in developing countries.