Hostname: page-component-77f85d65b8-6bnxx Total loading time: 0 Render date: 2026-04-12T21:19:23.343Z Has data issue: false hasContentIssue false
  • English
  • Français

Pyruvate kinase type M2: a crossroad in the tumor metabolome

Published online by Cambridge University Press:  09 March 2007

S. Mazurek ,
H. Grimm ,
C. B. Boschek ,
P. Vaupel  and
E. Eigenbrodt
S. Mazurek
Affiliation:
Institute for Biochemistry & Endocrinology, Veterinary Faculty, University of Giessen, Frankfurter Strasse 100, 35392 Giessen, Germany
H. Grimm
Affiliation:
Centre of Surgery, Medical Faculty, University of Giessen, Klinikstrasse 29, 35392 Giessen, Germany
C. B. Boschek
Affiliation:
Institute for Medical Virology, Medical Faculty, University of Giessen, Frankfurter Strasse 107, 35392 Giessen, Germany
P. Vaupel
Affiliation:
Institute of Physiology and Pathophysiology, Medical Faculty, University of Mainz, Duesbergweg 6, 55099 Mainz, Germany
E. Eigenbrodt*
Affiliation:
Institute for Biochemistry & Endocrinology, Veterinary Faculty, University of Giessen, Frankfurter Strasse 100, 35392 Giessen, Germany
*
*Corresponding author: Dr E. Eigenbrodt, fax +49 641 99 38179, email Erich.Eigenbrodt@vetmed.uni-giessen.de
Rights & Permissions [Opens in a new window]

Abstract

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'

Information

Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue S1 , January 2002 , pp. S23 - S29
Copyright
Copyright © The Nutrition Society 2002

References

Atkinson, DE & Fall, L (1967) Adenosine triphosphate conservation in biosynthetic regulation. The Journal of Biological Chemistry 10, 3241–3242.CrossRefGoogle Scholar
Atkinson, DE & Walton, GM (1967) Adenosine triphosphate conservation in metabolic regulation. The Journal of Biological Chemistry 10, 3239–3241.CrossRefGoogle Scholar
Aulwurm, UR & Brand, KA (2000) Increased formation of reactive oxygen species due to glucose depletion in primary cultures of rat thymocytes inhibits proliferation. European Journal of Biochemistry 267, 5693–5698.CrossRefGoogle ScholarPubMed
Bereiter-Hahn, J, Münnich, A & Woiteneck, P (1998) Dependence of energy metabolism on the density of cells in culture. Cell Structure and function 23, 85–93.CrossRefGoogle ScholarPubMed
Bond, CJ, Jurica, MS, Mesecar, A & Stoddard, BL (2000) Determinants of allosteric activation of yeast pyruvate kinase and identification of novel effectors using computational screening. Biochemistry 39, 15333–15343.CrossRefGoogle ScholarPubMed
Boros, LG, Puigjaner, J, Cascante, M, Lee, WNP, Brandes, JL, Bassilian, S, Yusuf, FI, Williams, RD, Muscarella, P, Melvin, WS & Schirmer, WJ (1997) Oxythiamine and dehydroepiandrosterone inhibit the nonoxidative synthesis of ribose and tumor cell proliferation. Cancer Research 57, 4242–4248.Google ScholarPubMed
Boscá, L & Corredor, C (1984) Is phosphofructokinase the rate limiting step of glycolysis?. Trends in Biochemical Sciences 9, 372–373.CrossRefGoogle Scholar
Brand, K, von Hintzenstern, J, Langer, K & Fekl, W (1987) Pathways of glutamine and glutamate metabolism in resting and proliferating rat thymocytes: Comparison between free and peptide-bound glutamine. Journal of Cellular Physiology 132, 559–564.CrossRefGoogle ScholarPubMed
Brazill, DT, Thorner, J & Martin, S (1997) Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae. Journal of Bacteriology 179, 4415–4418.CrossRefGoogle ScholarPubMed
Chesney, J, Mitchell, R, Benigni, F, Bacher, M, Spiegel, L, Al-Abed, Y, Han, JH, Metz, C & Bucala, R (1999) An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect. Proceedings of the National Academy of Sciences USA 96, 3047–3052.CrossRefGoogle ScholarPubMed
Chopade, BA, Shankar, S, Sundin, GW, Mukhopadhyay, S & Chakrabarty, AM (1997) Characterization of membrane-assciated Pseudomonas aeruginosa ras-like protein Pra, a GTP-binding protein that forms complexes with truncated nucleoside diphosphate kinase and pyruvate kinase to modulate GTP synthesis. Journal of Bacteriology 179, 2181–2188.CrossRefGoogle Scholar
Durante, P, Gueuning, MA, Darville, MI, Hue, L & Rousseau, GG (1999) Apoptosis induced by growth factor withdrawal in fibroblasts overproducing fructose 2,6-bisphosphate. FEBS Letters 448, 239–243.CrossRefGoogle ScholarPubMed
Eck, HP, Drings, P, Dröge, W (1989) Plasma glutamate levels, lymphocyte reactivity and death rate in patients with bronchial carcinoma. Journal of Cancer Research and Clinical Oncology 115, 571–574.CrossRefGoogle ScholarPubMed
Eigenbrodt, E, Fister, P & Reinacher, M (1985) New perspectives on carbohydrate metabolism in tumor cells. In Regulation of Carbohydrate Metabolism, pp. 141–179 [Beitner, R, editor]. Boca Raton, FL: CRC Press.Google Scholar
Eigenbrodt, E, Reinacher, M, Scheefers-Borchel, U, Scheefers, H & Friis, RR (1992) A double role for pyruvate kinase type M2 in the expansion of phosphometabolite pools found in tumor cells. In Critical Reviews in Oncogenesis, pp. 91–115 [Perucho, M, editor]. Boca Raton, FL: CRC Press.Google Scholar
Eigenbrodt, E, Gerbracht, U, Mazurek, S, Presek, P & Friis, R (1994) Carbohydrate metabolism and neoplasia: New perspectives for diagnosis and therapy. In Biochemical and Molecular Aspects of Selected Cancers, pp. 311–381 [Pretlow, TG and Pretlow, TP, editors]. San Diego: Academic Press volume 2.Google Scholar
Eigenbrodt, E, Kallinowski, F, Ott, M, Mazurek, S & Vaupel, P (1998a) Pyruvate kinase and the interaction of amino acid and carbohydrate metabolism in solid tumors. Anticancer Research 18, 3267–3274.Google ScholarPubMed
Eigenbrodt, E, Mazurek, S & Friis, RR (1998) Double role of pyruvate kinase type M2 in the regulation of phosphometabolite pools. In Cell Growth and Oncogenesis, pp. 15–30 [Bannasch, P, Kanduc, D, Papa, S and Tager, JM, editors]. Basel, Switzerland: Birkhäuser Verlag.CrossRefGoogle Scholar
Engel, M, Seifert, M, Theisinger, B, Seyfert, U & Welter, C (1998) Glyceraldehyde 3-phosphate dehydrogenase and Nm23-H1/nucleoside diphosphate kinase A. The Journal of Biological Chemistry 273, 20058–20065.CrossRefGoogle ScholarPubMed
Gosalvez, M, Pérez-García, J & Weinhouse, S (1974) Competition for ADP between pyruvate kinase and mitochondrial oxidative phosphorylation as a control mechanism in glycolysis. European Journal of Biochemistry 46, 133–140.CrossRefGoogle ScholarPubMed
Grimm, H, Tibell, A, Norrlind, B, Blecher, C, Wilker, S & Schwemmle, K (1994) Immunoregulation by parenteral lipids: impact of the n-3 to n-6 fatty acid ratio. Journal of Parenteral and Enteral Nutrition 18, 417–421.CrossRefGoogle ScholarPubMed
Grimm, H, Tibell, A, Norrlind, B, Schott, J & Bohle, RM (1995) Nutrition and allorejection impact of lipids. Transplant Immunology 3, 62–67.CrossRefGoogle ScholarPubMed
Hess, B (1963) Control of metabolic rates. In Control in Respiration and Fermentation, pp. 333–350 [Hess, B, Wright, B, editors]. New York: Ronald Press.Google Scholar
Jiang, WG, Bryce, RP & Horrobin, DF (1998) Essential fatty acids: molecular and cellular basis of their anti-cancer action and clinical implications. Critical Reviews in Oncology/Hematology 27, 179–209.CrossRefGoogle ScholarPubMed
Kallinowski, F, Fortmeyer, HP, Runkel, S & Vaupel, P (1987) Substrate utilization of human tumor xenografts in vivo. Journal of Nutritional Growth Cancer 4, 155–166.Google Scholar
Kallinowski, F, Vaupel, P, Runkel, S, Berg, G, Fortmeyer, HP, Baessler, KH & Wagner, K (1988) Glucose uptake, lactate release, ketone body turnover, metabolic micromilieu, and pH distributions in human breast cancer xenografts in nude rats. Cancer Research 48, 7264–7272.Google ScholarPubMed
Kew, S, Wells, SM, Yaqoob, P, Wallace, FA, Miles, EA & Calder, PC (1999) Dietary glutamine enhances murine T-lymphocyte responsiveness. Journal of Nutrition 129, 1524–1531.CrossRefGoogle ScholarPubMed
Lee, WNP, Byerley, LO, Bassilian, S, Ajie, HO, Clark, I, Edmond, J & Bergner, EA (1995) Isotopomer study of lipogenesis in human hepatoma cells in culture: contribution of carbon and reducing hydrogen from glucose. Analytica Biochemica 226, 100–112.CrossRefGoogle ScholarPubMed
Lee, WNP, Boros, LG, Puigjaner, J, Bassilian, S, Lim, S & Cascante, M (1998) Mass isotopomer study of the nonoxidative pathways of the pentose cycle with [1,2-13C2] glucose. American Journal of Physiology 274:E843–E851.Google ScholarPubMed
Live, TR & Kaminskas, E (1975) Changes in adenylate energy charge in Ehrlich ascites tumor cells deprived of serum, glucose, or amino acids. The Journal of Biological Chemistry 250, 1786–1789.CrossRefGoogle ScholarPubMed
Luesink, EJ, Beumer, CMA, Kuipers, OP & De Vos, WM (1999) Molecular characterization of the lactococcus lactis ptsH1 operon and analysis of the regulatory role of HPr. Journal of Bacteriology 181, 764–771.CrossRefGoogle Scholar
Maeba, P & Sanwal, BD (1968) The regulation of pyruvate kinase of Escherichia coli by fructose diphosphate and adenylic acid. The Journal of Biological Chemistry 243, 448–450.CrossRefGoogle ScholarPubMed
Marchut, E, Gumínska, M & Kķdryna, T (1986) The inhibitory effect of various fatty acids on aerobic glycolysis in Ehrlich ascites tumour cells. Acta Biochimica Polonica 33, 7–16.Google ScholarPubMed
Mazurek, S, Hugo, F, Failing, K & Eigenbrodt, E (1996) Studies on associations of glycolytic and glutaminolytic enzymes in MCF-7 cells: role of p36. Journal of Cellular Physiology 167, 238–250.3.0.CO;2-Q>CrossRefGoogle ScholarPubMed
Mazurek, S, Boschek, CB & Eigenbrodt, E (1997a) The role of phosphometabolites in cell proliferation, energy metabolism, and tumor therapy. Journal of Bioenergetics and Biomembranes 29, 315–330.CrossRefGoogle ScholarPubMed
Mazurek, S, Michel, A & Eigenbrodt, E (1997b) Effect of extracellular AMP on cell proliferation and metabolism of breast cancer cell lines with high and low glycolytic rates. The Journal of Biological Chemistry 272, 4941–4952.CrossRefGoogle ScholarPubMed
Mazurek, S, Grimm, H, Wilker, S, Leib, S & Eigenbrodt, E (1998) Metabolic characteristics of different malignant cancer cell lines. Anticancer Research 18, 3275–3282.Google ScholarPubMed
Mazurek, S, Weise, G, Wüst, G, Schäfer-Schwebel, A, Eigenbrodt, E & Friis, RR (1999a) Energy metabolism in the involuting mammary gland. in vivo 13, 467–478.Google ScholarPubMed
Mazurek, S, Eigenbrodt, E, Failing, K & Steinberg, P (1999b) Alterations in the glycolytic and glutaminolytic pathways after malignant transformation of rat liver oval cells. Journal of Cellular Physiology 181, 136–146.3.0.CO;2-T>CrossRefGoogle ScholarPubMed
Mazurek, S, Grimm, H, Oehmke, M, Weisse, G, Teigelkamp, S & Eigenbrodt, E (2000) Tumor M2-PK and glutaminolytic enzymes in the metabolic shift of tumor cells. Anticancer Research 20, 5151–5154.Google ScholarPubMed
Mazurek, S, Zwerschke, W, Jansen-Dürr, P & Eigenbrodt, E (2001) Effects of the HPV-16 E7 oncoprotein on glycolysis and glutaminolysis: Role of pyruvate kinase type M2 and the glycolytic enzyme complex. Biochemical Journal 356, 247–256.CrossRefGoogle ScholarPubMed
McKeehan, WL (1982) Glycolysis, glutaminolysis and cell proliferation. Cell Biology International Reports 6, 635–650.CrossRefGoogle ScholarPubMed
Melo, RF, Stevan, FR, Campello, AP, Carnieri, EGS & Martinelli de Oliveira, MB (1998) Occurrence of the crabtree effect in Hela cells. Cell Biochemistry and Function 16, 99–105.3.0.CO;2-2>CrossRefGoogle ScholarPubMed
Nelson, DL & Kornberg, A (1970a) Biochemical studies of bacterial sporulation and germination. Phosphate metabolism during sporulation. The Journal of Biological Chemistry 245, 1137–1145.CrossRefGoogle ScholarPubMed
Nelson, DL & Kornberg, A (1970b) Biochemical studies of bacterial sporulation and germination. Phosphate metabolism during germination. The Journal of Biological Chemistry 245, 1146–1155.CrossRefGoogle ScholarPubMed
Newsholme, EA & Calder, PC (1997) The proposed role of glutamine in some cells of the immune system and speculative consequences for the whole animal. Nutrition 13, 728–730.CrossRefGoogle ScholarPubMed
Nguyen, TN, Wang, HJ, Zalzal, S, Nanci, A & Nabi, IR (2000) Purification and characterization of beta-actin-rich tumor cell pseudopodia: role of glycolysis. Experimental Cell Research 258, 171–183.CrossRefGoogle ScholarPubMed
Oremek, GM, Teigelkamp, S, Kramer, W, Eigenbrodt, E, Usadel, K-H (1999) The pyruvate kinase isoenzyme tumor M2 (Tu M2-PK) as a tumor marker for renal carcinoma. Anticancer Research 19, 2599–2602.Google ScholarPubMed
Perez, JX, Roig, T, Manzano, A, Dalmau, M, Boada, J, Ventura, F, Rosa, JL, Bermudez, J & Bartrons, R (2000) Overexpression of fructose 2,6-bisphosphatase decreases glycolysis and delays cell cycle progression. American Journal of Physiological Cell Physiology 279, C1359–C1365.CrossRefGoogle ScholarPubMed
Pizer, ES, Chrest, FJ, DiGiuseppe, JA & Han, WF (1998) Pharmacological inhibitors of mammalian fatty acid synthase suppress DNA replication and induce apoptosis in tumor cell lines. Cancer Research 58, 4611–4615.Google ScholarPubMed
Popanda, O, Fox, G & Thielmann, HW (1998) Modulation of DNA polymerases α, δ and ε by lactate dehydrogenase and 3-phosphoglycerate kinase. Biochimica et Biophysica Acta 1397, 102–117.CrossRefGoogle ScholarPubMed
Presek, P, Reinacher, M & Eigenbrodt, E (1988) Pyruvate kinase type M2 is phosphorylated at tyrosine residues in cells transformed by Rous sarcoma virus. FEBS Letters 242, 194–198.CrossRefGoogle ScholarPubMed
Punnonen, K, Hietanen, E, Auvinen, O & Punnonen, R (1989) Phospholipids and fatty acids in breast cancer tissue. Journal of Cancer Research and Clinical Oncology 115, 575–578.CrossRefGoogle ScholarPubMed
Rhaese, H-J, Grade, R & Dichtelmüller, H (1976) Studies on the control of development. European Journal of Biochemistry 64, 205–213.CrossRefGoogle ScholarPubMed
Schramm, A, Siebers, B, Tjaden, B, Brinkmann, H & Hensel, R (2000) Pyruvate kinase of the hyperthermophilic Crenarchaeote Thermoproteus tenax: Physiological role and phylogenetic aspects. Journal of Bacteriology 182, 2001–2009.CrossRefGoogle ScholarPubMed
Sebolt, JS & Weber, G (1984) Negative correlation of L-glutamine concentration with proliferation rate in rat hepatomas. Life Sciences 34, 301–306.CrossRefGoogle ScholarPubMed
Shim, H, Chun, YS, Lewis, BC & Dang, CV (1998) A unique glucose dependent apoptotic pathway induced by c-Myc. Proceedings of the National Academy of Sciences, USA 95, 1511–1516.CrossRefGoogle ScholarPubMed
Teusink, B, Walsh, MC, van Dam, K & Westerhoff, HV (1998) The danger of metabolic pathways with turbo design. Trends in Biochemical Sciences 23, 162–169.CrossRefGoogle ScholarPubMed
Thevelein, JM & Hohmann, S (1995) Trehalose synthase: guard to the gate of glycolysis in yeast?. Trends in Biochemical Sciences 20, 3–10.CrossRefGoogle Scholar
Thomas, S & Fell, DA (1998) A control analysis exploration of the role of ATP utilisation in glycolytic-flux control and glycolytic metabolite concentration regulation. European Journal of Biochemistry 258, 956–967.CrossRefGoogle ScholarPubMed
Tuominen, FW & Bernlohr, RW (1971) Pyruvate kinase of the spore-forming bacterium, Bacillus licheniformis. The Journal of Biological Chemistry 246, 1746–1755.CrossRefGoogle ScholarPubMed
Valle, F, Muňoz, E, Ponce, E, Flores, N & Bolivar, F (1996) Basic and applied aspects of metabolic diversity: the phosphoenolpyruvate node. Journal of Industrial Microbiology 17, 458–462.Google Scholar
Vaupel, P, Fortmeyer, HP, Runkel, S & Kallinowski, F (1987) Blood flow, oxygen consumption, and tissue oxygenation of human breast cancer xenografts in nude rats. Cancer Research 47, 3496–3503.Google ScholarPubMed
Wang, H & Iynedjian, PB (1997) Acute glucose intolerance in insulinoma cells with unbalanced overexpression of glucokinase. The Journal of Biological Chemistry 272, 25731–25736.CrossRefGoogle ScholarPubMed
Wheeler, MD, Ikejema, K, Enomoto, N, Stacklewitz, RF, Seabra, V, Zhong, Z, Yin, M, Schemmer, P, Rose, ML, Rusyn, I, Bradfprd, B & Thurman, RG (1999) Glycine: a new anti-inflammatory immunonutrient. Cellular Molecular Life Sciences 56, 843–856.CrossRefGoogle ScholarPubMed
Zwerschke, W, Mazurek, S, Massimi, P, Banks, L, Eigenbrodt, E, Jansen-Dürr, P (1999) Modulation of type M2 pyruvate kinase activity by the human papillomavirus type 16 E7 oncoprotein. Proceedings of the National Academy of Sciences, USA 96, 1291–1296.CrossRefGoogle ScholarPubMed