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Cryptosporidium species and subtype analysis from dairy calves in Spain

Published online by Cambridge University Press:  04 November 2008

J. QUILEZ ,
E. TORRES ,
R. M. CHALMERS ,
G. ROBINSON ,
E. DEL CACHO  and
C. SANCHEZ-ACEDO
J. QUILEZ*
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain
E. TORRES
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain
R. M. CHALMERS
Affiliation:
UK Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea SA2 8QA, UK
G. ROBINSON
Affiliation:
UK Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea SA2 8QA, UK
E. DEL CACHO
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain
C. SANCHEZ-ACEDO
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain
*
*Corresponding author: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain. Tel: +34 976 762150. Fax: +34 976 761612. E-mail: jquilez@unizar.es
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Summary

Faecal specimens from 287 diarrhoeic calves younger than 21 days, collected over a 2-year period (2006–2007) from 82 dairy cattle farms in 14 provinces across the north of Spain, were examined for the presence of Cryptosporidium oocysts. Overall, 63 farms (76·8%) and 166 calves (57·8%) tested positive by microscopy. In order to elucidate the genetic diversity, selected positive specimens from 149 calves originating from 61 farms in the 14 provinces were examined by genotyping and subtyping techniques. Cryptosporidium parvum was the only species identified by PCR-RFLP of SSU rDNA from all 149 isolates and sequencing of a subset of 50 isolates, except for 2 specimens that were identified as C. bovis. Sequence analyses of the glycoprotein (GP60) gene revealed that most C. parvum isolates (98%) belonged to the subtype family IIa and 2 isolates were identified as the novel subtype IIdA23G1. Subtype IIaA15G2R1 was the most common and widely distributed (80·3% of the 61 farms), followed by subtype IIaA16G3R1 (14·7%), whereas the remaining IIa subtypes (IIaA16G2R1, IIaA17G2R1, IIaA18G3R1, IIaA19G3R1) were restricted to 1–3 farms. All these C. parvum IIa subtypes have previously been described in human patients, indicating that most isolates from diarrhoeic calves in northern Spain have zoonotic potential.

Keywords

Cryptosporidiumdairy calvesPCR-RFLPGP60 subtypesSpain

Information

Type
Research Article
Information
Parasitology , Volume 135 , Issue 14 , December 2008 , pp. 1613 - 1620
Copyright
Copyright © 2008 Cambridge University Press

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