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The association between cannabis and psychosis is established, but the role of underlying genetics is unclear. We used data from the EU-GEI case-control study and UK Biobank to examine the independent and combined effect of heavy cannabis use and schizophrenia polygenic risk score (PRS) on risk for psychosis.
Methods
Genome-wide association study summary statistics from the Psychiatric Genomics Consortium and the Genomic Psychiatry Cohort were used to calculate schizophrenia and cannabis use disorder (CUD) PRS for 1098 participants from the EU-GEI study and 143600 from the UK Biobank. Both datasets had information on cannabis use.
Results
In both samples, schizophrenia PRS and cannabis use independently increased risk of psychosis. Schizophrenia PRS was not associated with patterns of cannabis use in the EU-GEI cases or controls or UK Biobank cases. It was associated with lifetime and daily cannabis use among UK Biobank participants without psychosis, but the effect was substantially reduced when CUD PRS was included in the model. In the EU-GEI sample, regular users of high-potency cannabis had the highest odds of being a case independently of schizophrenia PRS (OR daily use high-potency cannabis adjusted for PRS = 5.09, 95% CI 3.08–8.43, p = 3.21 × 10−10). We found no evidence of interaction between schizophrenia PRS and patterns of cannabis use.
Conclusions
Regular use of high-potency cannabis remains a strong predictor of psychotic disorder independently of schizophrenia PRS, which does not seem to be associated with heavy cannabis use. These are important findings at a time of increasing use and potency of cannabis worldwide.
Many clinical trials leverage real-world data. Typically, these data are manually abstracted from electronic health records (EHRs) and entered into electronic case report forms (CRFs), a time and labor-intensive process that is also error-prone and may miss information. Automated transfer of data from EHRs to eCRFs has the potential to reduce data abstraction and entry burden as well as improve data quality and safety.
Methods:
We conducted a test of automated EHR-to-CRF data transfer for 40 participants in a clinical trial of hospitalized COVID-19 patients. We determined which coordinator-entered data could be automated from the EHR (coverage), and the frequency with which the values from the automated EHR feed and values entered by study personnel for the actual study matched exactly (concordance).
Results:
The automated EHR feed populated 10,081/11,952 (84%) coordinator-completed values. For fields where both the automation and study personnel provided data, the values matched exactly 89% of the time. Highest concordance was for daily lab results (94%), which also required the most personnel resources (30 minutes per participant). In a detailed analysis of 196 instances where personnel and automation entered values differed, both a study coordinator and a data analyst agreed that 152 (78%) instances were a result of data entry error.
Conclusions:
An automated EHR feed has the potential to significantly decrease study personnel effort while improving the accuracy of CRF data.
The purpose of this study was to describe the longitudinal trajectories and bidirectional relationships of the physical-social and emotional functioning (EF) dimensions of positive aging and to identify their baseline characteristics.
Methods:
Women age 65 and older who enrolled in one or more Women's Health Initiative clinical trials (WHI CTs) and who had positive aging indicators measured at baseline and years 1, 3, 6, and 9 were included in these analyses (N = 2281). Analytic strategies included latent class growth modeling to identify longitudinal trajectories and multinomial logistic regression to examine the effects of baseline predictors on these trajectories.
Results:
A five-trajectory model was chosen to best represent the data. For Physical-Social Functioning (PSF), trajectory groups included Low Maintainer (8.3%), Mid-Low Improver (10.4%), Medium Decliner (10.7%), Mid-High Maintainer (31.2%), and High Maintainer (39.4%); for EF, trajectories included Low Maintainer (3%), Mid-Low Improver (9%), Medium Decliner (7.7%), Mid-High Maintainer (22.8%), and High Maintainer (57.5%). Cross-classification of the groups of trajectories demonstrated that the impact of a high and stable EF on PSF might be greater than the reverse. Low depression symptoms, low pain, and high social support were the most consistent predictors of high EF trajectories.
Conclusion:
Aging women are heterogeneous in terms of positive aging indicators for up to 9 years of follow-up. Interventions aimed at promoting sustainable EF might have diffused effects on other domains of healthy aging.
To identify risk factors associated with methicillin-resistant Staphylococcus aureus (MRSA) acquisition in long-term care facility (LTCF) residents.
Design.
Multicenter, prospective cohort followed over 6 months.
Setting.
Three Veterans Affairs (VA) LTCFs.
Participants.
All current and new residents except those with short stay (<2 weeks).
Methods.
MRSA carriage was assessed by serial nares cultures and classified into 3 groups: persistent (all cultures positive), intermittent (at least 1 but not all cultures positive), and noncarrier (no cultures positive). MRSA acquisition was defined by an initial negative culture followed by more than 2 positive cultures with no subsequent negative cultures. Epidemiologic data were collected to identify risk factors, and MRSA isolates were typed by pulsed-field gel electrophoresis (PFGE).
Results.
Among 412 residents at 3 LTCFs, overall MRSA prevalence was 58%, with similar distributions of carriage at all 3 facilities: 20% persistent, 39% intermittent, 41% noncarriers. Of 254 residents with an initial negative swab, 25 (10%) acquired MRSA over the 6 months; rates were similar at all 3 LTCFs, with no clusters evident. Multivariable analysis demonstrated that receipt of systemic antimicrobials during the study was the only significant risk factor for MRSA acquisition (odds ratio, 7.8 [95% confidence interval, 2.1–28.6]; P = .002). MRSA strains from acquisitions were related by PFGE to those from a roommate in 9/25 (36%) cases; 6 of these 9 roommate sources were persistent carriers.
Conclusions.
MRSA colonization prevalence was high at 3 separate VA LTCFs. MRSA acquisition was strongly associated with antimicrobial exposure. Roommate sources were often persistent carriers, but transmission from roommates accounted for only approximately one-third of MRSA acquisitions.
LC50 and LT50 values for two multicapsid nuclear polyhedrosis viruses isolated from the alfalfa looper, Autographa californica (Speyer), and a mint looper, Rachiplusia ou (Guenée), were determined against black cutworm, Agrotis ipsilon (Hufnagel), neonate larvae, 1-day-old larvae reared at 15 °C before testing, and 1-day-old larvae reared at 27 °C before testing. The results showed that black cutworm larvae have low to moderate susceptibility to these viruses. As the larvae developed, their susceptibility to the viruses rapidly declined. Initial growth of larvae surviving sublethal dosages of these viruses was reduced, but pupal weights of these larvae were not significantly different from untreated controls.
A study was conducted to determine whether intramammary antibiotic treatment of heifer mammary glands following the first milking after calving was effective for reducing the percentage of mammary quarters infected during early lactation. Jersey and Holstein heifers from two research herds were assigned to one of three treatment groups: (1) no intramammary infusion following the first milking after parturition, (2) intramammary infusion of all quarters with pirlimycin hydrochloride following the first milking after parturition and (3) intramammary infusion of all quarters with novobiocin sodium plus penicillin G procaine following the first milking after parturition. Almost 93% of Jersey heifers (40/43) and 73·1% of quarters (125/171) were infected at the first milking. Almost 77% of quarters (33/43) were cured following treatment with pirlimycin, 61·8% (21/34) were cured following treatment with penicillin-novobiocin and 39·6% (19/48) of infections were eliminated spontaneously in the untreated control group. Significantly fewer infections were observed in pirlimycin or penicillin-novobiocin treated mammary glands of Jersey heifers during early lactation than in untreated control mammary glands. Almost 89% of Holstein heifers (32/36) and 52·8% of quarters (76/144) were infected at the first milking. About 57% (12/21) of quarters were cured following treatment with pirlimycin, 41·4% (12/29) were cured following treatment with penicillin-novobiocin and 23·1% (6/26) of infections were eliminated spontaneously in the untreated negative control group. Significantly fewer infections were observed in pirlimycin treated mammary glands of Holstein heifers during early lactation than in untreated control mammary glands. However, no significant differences were observed following penicillin-novobiocin treatment of Holstein heifers after the first milking of lactation compared with untreated control quarters. Coagulase-negative staphylococci, Streptococcus uberis and Streptococcus dysgalactiae subsp dysgalactiae were isolated most frequently in heifers from both herds.
The present paper reports on the phonological form of one child's
productive vocabulary from age 0;10 to 1;8 with primary focus on his
production of multisyllabic targets. A large percentage of his multisyllabic
vocabulary was produced as one syllable until the age of 1;6.
This limitation was not due to a tendency to extract only single syllables
from the speech stream, but rather due primarily to a limitation on
production. While some portion of his one-syllable productions could be
interpreted as the result of single syllable extraction, a sizeable portion
affirmed that he extracted the target size correctly by his inclusion of first
and final target phonemes in his productions (e.g. [po] for piano and
[kiz] for candies). The resolution of this limitation coincides with his
move toward two-word speech. We conclude that there is a developmental and
perhaps maturational limitation in the capacity to carry
out the processes underlying word and sentence production.
This paper reports on the use of frequency as a control parameter in the generation of microwave-excited plasmas for applications such as chemical vapor deposition, chemical vapor infiltration, or plasma etching. By changing the excitation frequency of a plasma in a multi-mode chamber, the locations of the maxima and minima of the power density within the chamber are also changed. This allows localization of the plasma discharge in the precise processing area desired in order to increase the process' efficiency. In a similar fashion, sweeping of the processing frequency during the process cycle can theoretically “scan” the plasma across an arbitrarily-shaped target surface. A series of experiments was conducted to evaluate the flexibility of variable frequency microwave energy as a plasma process tool. The results of these tests are presented.
Milk quotas, based on an average fat content, severely limit milk production on UK farms. Predictions of the time-course of lactation are incorporated into most computerized herd management programs but these models take no account of food inputs, body weight change or milk composition. Dynamic models are generally used to simulate metabolic pathways and, as such, have little direct relevance to commercial milk production. Dynamic models can be converted to an adaptive-predictive model that partitions food energy into milk and non-milk constituents. This paper reports the development of an adaptive-predictive model to partition food into milk and non-milk components. Additional functions further partition milk energy into the principal constituents, fat, protein and lactose.
Manipulations of the lactation cycle are achieved through nutritional regimens and generally result in a restriction of the physiological lactation cycle. Most models of lactation used in management programmes only describe the average time course of lactation for groups of cows on a given feeding regimen. This is inadequate for the variety of feeding systems in use on British farms. In the present paper several existing models of lactation have been critically evaluated. An integral equation approach has been developed to rationalize experimental observations and currently accepted biological concepts.
Four vitamin B12 assays were compared using blood sera from Friesian cows on winter diets or grazing. In herd 1, ten animals were blood-sampled three times at monthly intervals and the vitamin B12 concentration of the sera determined by the Poteriochromonas malhamensis and Lactobacillus delbrueckii assays. At all three sampling dates the results produced by the P. malhamensis assay were significantly greater than those produced by the L. delbrueckii assay. Cows in herd 2 were divided into two groups, each of 30 animals. One group was given a soluble glass bolus releasing cobalt and the other was unsupplemented. Milk yields were recorded throughout the experimental period and herbage samples were taken at intervals for cobalt determinations. Blood samples were taken at intervals from January to September 1983 and the vitamin B12 concentration of the sera determined by four different assay methods: the P. malhamensis and L. delbrueckii microbiological assays and the Becton Dickinson and RIA Products ‘No-boil’ radioassays. The last of these failed to detect vitamin B12 in any sample. There was a significant difference between the results obtained by the three other assays, with the Becton Dickinson radioassay consistently producing the lowest result and the P. malhamensis assay the highest result of the three. There was no significant effect of cobalt supplementation on milk yield.
TwO Latin-square experiments using lactating Jersey cows have been made to determine the response to 4 levels of acetic acid given as a supplement to diets fed at Woodman's Standards. The response in milk yield and composition to daily supplementary infusions of acetic acid is curvilinear, the point of inflexion occurring between the levels of 450 and 600 ml acetic acid/d. The direction of responses to the 150-ml level of infusion varied between the experiments. At the 600-ml level of addition the non-significant increases in milk fat, protein and lactose yield led to a significant increase in solids corrected milk yield. The effects of acetic acid infusion on the composition of the milk fat were determined.
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