Objectives/Goals: Chronic stress may accelerate biological aging yet is often overlooked in clinical settings. Many tools to assess stress exist, but a comprehensive measure of cumulative stress across the lifespan is unavailable. This study validates a novel measure of lifetime stress for use as a screening tool in clinical practice. Methods/Study Population: Patients (n > 220) enrolled in brain health research registry at the Washington University St. Louis Knight Alzheimer Disease Research Center completed in-person surveys at baseline and after six months. Baseline measures included the everyday discrimination scale (EDS), total adverse experience (TAE), and demographics. Age and evaluating life course stress experience (ELSE) scores were measured six months later. Ongoing analysis includes age-adjusted correlations of ELSE scores with TAE and EDS scores. We will investigate the correlation with race and ethnicity and sex assigned at birth. We will explore the relationship between ELSE score and multidimensional intersectionality. Results/Anticipated Results: The sample was 87% Black or African American, 8% White, 4% Hispanic, 82% female, and 18% male, with a mean age of 66 ± 10 years. Age-adjusted relationships between patient characteristics and ELSE scores will be analyzed. Additionally, ELSE responses will be compared against age, EDS, and TAE measurements. Intersectionality between race-ethnicity, sex, and gender will be examined. We hypothesize ELSE scores will vary by demographic. Preliminary results indicate the ELSE scale correlates with established life stress measures, accounting for cumulative stress exposure across a lifespan independent of specific stressor topics. Discussion/Significance of Impact: The ELSE scale is a viable tool for clinical screening of chronic stress exposure over a lifespan. Its implementation will allow clinicians to identify patients at high risk for accelerated aging, facilitating targeted interventions and advancing equity in healthcare delivery.

This analysis demonstrates theoretically that a lateral bending wave propagating along the walls of a two-dimensional channel filled with a viscous incompressible fluid will induce a mean flow. In addition to this ‘pure’ bending wave, another possible condition is investigated: that of the superposition of an area contraction wave propagating with the same speed as the lateral bending wave. This second condition, called complex wave motion, takes into account the slight occlusion which occurs naturally at the amplitude peaks when a finite amplitude bending wave propagates along the walls of a container. A perturbation solution is found which satisfies Navier—Stokes equations for the case in which wave amplitude/wavelength is ‘small’. However the wave amplitude is finite, in the sense that it is of the same order as the channel width. Under these conditions, the occlusion at the amplitude peaks is allowed to be of the same order as the channel width. For the case of a pure bending wave the motion induced by the peristaltis is found to be of second order in the perturbation parameter, whereas in the more realistic case a first-order pumping effect is obtained.

Electrothermal-Chemical Synthesis (ETCS) is a relatively new process for the synthesis of nanocrystalline powders. With ETCS nanoparticles are formed through the controlled chemical reaction of a metal plasma, produced by cathode erosion, with a gas. By controlling the quench rate of the plasma precisely, the average particle size can be varied from as small as 6–8 nm up to 100 nm and above. The process is quite versatile and can be used to produce a wide variety of nanopowders including metals, oxides, and nitrides. However, before the nanoparticles produced by this process (or any other process for that matter) can find wide scale commercial acceptance, their physical properties must be characterized. Among many characterization techniques, electron microscopy can provide structural, chemical, and morphological information on nanoparticles. In this paper, we will discuss the SEM and TEM characterization of metal and metal oxide nanoparticles powders produced by ETCS.

The correspondence decision problem was first formulated and shown to be recursively unsolvable in Post (1946). The method of proof was to reduce the known unsolvable decision problem for the class of normal systems on a, b to the correspondence decision problem. In the present paper the concept of a standard Post normal system is used so as to obtain some equivalence reductions of combinatorial systems. In particular the following main result is obtained.