This paper studies twisted signature invariants and twisted linking forms, with a view toward obstructions to knot concordance. Given a knot K and a representation
$\rho $ of the knot group, we define a twisted signature function
$\sigma _{K,\rho } \colon S^1 \to \mathbb {Z}$. This invariant satisfies many of the same algebraic properties as the classical Levine-Tristram signature
$\sigma _K$. When the representation is abelian,
$\sigma _{K,\rho }$ recovers
$\sigma _K$, while for appropriate metabelian representations,
$\sigma _{K,\rho }$ is closely related to the Casson-Gordon invariants. Additionally, we prove satellite formulas for
$\sigma _{K,\rho }$ and for twisted Blanchfield forms.