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Freshwater mussels are declining rapidly worldwide. Propagation has the potential to restore numbers of these remarkable organisms, preventing extinction of rare species and maintaining the many benefits that they bring to aquatic ecosystems. Written by practitioners with firsthand experience of propagation programs, this practical book is a thorough guide to the subject, taking readers through the process from start to finish. The latest propagation and culture techniques are explored as readers follow freshwater mussels through their amazing and complex life cycle. Topics covered include the basics of building a culture facility, collecting and maintaining brood stock, collecting host species, infesting host species with larval mussels, collecting and culturing juvenile mussels, releasing juveniles to the wild, and post-release monitoring. This will be valuable reading for any biologist interested in the conservation of freshwater mussel populations.
The effects on leucine uptake and its incorporation into protein by cucumber (Cucumis sativus L. ‘Ashley’) cotyledon tissue following treatments with 2,4-bis(isopropylamino)-6-(methylthio)-s-triazine (prometryne) were determined. Prometryne decreased 14C-leucine uptake both in the light and in the dark. Prometryne decreased leucine incorporation into protein only when the discs were also exposed to over 4 hr of light. Percent of absorbed leucine incorporated into protein was increased by a 4 hr exposure in the light to 10−4M prometryne following a 24-hr dark exposure to this same concentration. Similar exposure to 10~5M prometryne had no effect. Percent of leucine incorporated into protein was decreased by 10-5M prometryne following a 24 hr exposure to this same concentration in the light. However, under these conditions 10−4M prometryne had no effect. It is speculated that the increased percentage of leucine incorporated into protein may be related to increased availability of the absorbed leucine for use in protein synthesis rather than an effect of the herbicide on the rate of protein synthesis