In summer, 2011, we investigated suspected glyphosate-resistant (GR) kochiain three chem-fallow fields (designated F1, F2, F3, each farmed by adifferent grower) in southern Alberta. This study characterizes glyphosateresistance in those populations, based on data from dose–responseexperiments. In a greenhouse experiment, the three populations exhibited aresistance factor ranging from 4 to 6 based on shoot biomass response (GR50 ratios), or 5 to 7 based on survival response (LD50 ratios). Similar results were found in a fielddose–response experiment at Lethbridge, AB, in spring 2012 using the F2kochia population. In fall 2011, we surveyed 46 fields within a 20-km radiusof the three chem-fallow fields for GR kochia. In the greenhouse,populations were screened with glyphosate at 900 g ae ha−1. Sevenpopulations were confirmed as GR, the farthest site located about 13 km fromthe three originally confirmed populations. An additional GR population morethan 100 km away was later confirmed. Populations were screened foracetolactate synthase (ALS)–inhibitor (thifensulfuron : tribenuron) anddicamba resistance in the greenhouse, with molecular characterization ofALS-inhibitor resistance in the F1, F2, and F3 populations. All GRpopulations were resistant to the ALS-inhibiting herbicide, but susceptibleto dicamba. ALS-inhibitor resistance in kochia was conferred by Pro197, Asp376, or Trp574 amino acidsubstitutions. Based upon a simple empirical model with a parameter forselection pressure, calculated from weed relative abundance and glyphosateefficacy, and a parameter for seedbank longevity, kochia, wild oat, andgreen foxtail were the top three weeds, respectively, predicted at risk ofselection for glyphosate resistance in the semiarid Grassland region of theCanadian prairies; wild oat, green foxtail, and cleavers species werepredicted at greatest risk in the subhumid Parkland region. This studyconfirms the first occurrence of a GR weed in western Canada. Futureresearch on GR kochia will include monitoring, biology and ecology, fitness,mechanism of resistance, and best management practices.