Mesotrione, topramezone, and tembotrione inhibit 4-hydroxyphenylpyruvatedioxygenase (HPPD), an enzyme integral to carotenoid biosynthesis. Researchwas conducted to evaluate the response of hybrid bermudagrass followingmesotrione (280, 350, and 420 g ai ha−1), topramezone (18, 25,and 38 g ai ha−1), and tembotrione (92, 184, and 276 g ai ha−1) applications. Measurements of visual bleaching (VB) andchlorophyll fluorescence yield (Fv/Fm) were evaluated3, 5, 7, 14, 21, 28, and 35 d after application (DAA). Leaf tissues weresampled on the same dates and assayed for chlorophyll and carotenoidpigments using high-performance liquid chromatography (HPLC). Responses ofplants treated with topramezone and tembotrione were similar; theseherbicides caused more VB and greater reductions in Fv/Fm, total chlorophyll, lutein, and xanthophyllcycle pigment concentrations than mesotrione 5 to 21 DAA. Increasingmesotrione application rate did not increase VB or lead to greaterreductions in total chlorophyll, lutein, or xanthophyll pigmentconcentrations. Alternatively, increasing topramezone and tembotrioneapplication rates above 18 and 92 g ha−1, respectively, extendedVB and pigment reductions. Of the three HPPD-inhibitors tested, topramezonewas the most active, because the low (18 g ha−1) rate oftopramezone reduced lutein and total xanthophyll pigment concentrations morethan the low rate of tembotrione (92 g ha−1) during periods ofmaximum activity (14 to 21 DAA). No necrosis was observed with any of thetreatments, suggesting tank mixtures of topramezone with other herbicidesmight be required to provide long-term control of hybrid bermudagrass.