Weeds are often spatially aggregated in maize fields, and the level ofaggregation varies across and within fields. Several annual weed species arepresent in maize fields before postemergence herbicide application, andherbicides applied will control several species at a time. The goal of thisstudy was to assess the spatial distribution of multispecies weedinfestation in maize fields. Ground-based imagery was used to map weedinfestations in rain-fed maize fields. Image segmentation was used toextract weed cover information from geocoded images, and an expert-basedthreshold of 0.102% weed cover was used to generate maps of weedpresence/absence. From 19 site-years, 13 (68%) demonstrated a random spatialdistribution, whereas six site-years demonstrated an aggregated spatialpattern of either monocotyledons, dicotyledons, or both groups. The resultsof this study indicated that monocotyledonous and dicotyledonous weed groupswere not spatially segregated, but discriminating these weed groups slightlyincreased the chances of detecting an aggregated pattern. It was concludedthat weeds were not always spatially aggregated in maize fields. Thesefindings emphasize the need for techniques allowing the assessment of weedaggregation prior to conducting site-specific weed management.