A soil bioassay experiment was conducted in Blacksburg, VA, to determine the effect of isoxaben application timings and rates on duration of weed control. Flats containing soil were imbedded into the field. Isoxaben was applied in the spring, fall, and spring followed by fall (double application) at 0.56, 0.84, and 1.12 kg ai/ha. Flats were moved to a greenhouse at 0, 1, 3, 6, and 9 months after treatment (MAT) and seeded with yellow rocket, buckhorn plantain, and spotted spurge for the bioassays. Weed counts from treated flats were compared to those in untreated flats to determine percent control. Fall and spring followed by fall applications provided approximately 20% greater control of yellow rocket 3 MAT and 30% greater control 6 MAT, compared to a single spring application of isoxaben. Isoxaben at all rates controlled yellow rocket > 70% at 6 mo after fall application. At 3 MAT, fall and spring plus fall-applied isoxaben provided about 15 and 20% greater buckhorn plantain control, respectively, compared to spring application. The two highest rates of isoxaben controlled buckhorn plantain > 70% at 3 mo after fall application, but provided poor control at 6 MAT. Control of spotted spurge was similar among the three application timings for isoxaben and was unacceptable at most evaluation dates. Overall, isoxaben applied at 1.12 kg/ha provided better control of all three weed species for a longer time than the reference herbicide, oxadiazon, applied at 3.36 kg/ha.