Allosteric communication is established by networks through which strain energy generated at the allosteric site by an allosteric event, such as ligand binding, can propagate to the functional site. Exerted on multiple molecules in the cell, it can wield a biased function. Here, we discuss allosteric networks and allosteric signaling bias. Networks are graphs specified by nodes (residues) and edges (their connections). Allosteric bias is a property of a population. It is described by allosteric effector-specific dynamic distributions of conformational ensembles, as classically exemplified by G protein-coupled receptors (GPCRs). An ensemble describes the likelihood of a specific (strong/weak) allosteric signal propagating to a specific functional site. A network description provides the propagation route in a specific conformation, pinpointing key residues whose mutations could promote drug resistance. Efficiency is influenced by path length, relative stabilities and allosteric transitions. Through specific contacts, specific ligands can bias signaling in proteins, for example, in receptor tyrosine kinases (RTKs) toward specific phosphorylation sites and cell signaling activation. Thus, rather than the two – active and inactive – states, and a single pathway, we consider multiple states and favored pathways. This allows us to consider biased allosteric switches among minor, invisible states and observable outcomes. Within this framework, we further consider signaling strength and duration as key determinants of cell fate: If weak and sustained, it may induce differentiation; If bursts of strong and short, proliferation.

Recent progress in using real-time kinematic (RTK) positioning has motivated the exploration of its application due to its high accuracy and efficiency. However, poorly-observed satellite data will cause unfixed ambiguities and markedly biased solutions. A novel partial ambiguity resolution method, named the irrespective of integer ambiguity resolution (IIAR) model, is proposed and applied to improve the reliability of ambiguity resolution. The proposed method contains initial ambiguity resolution and irrespective of integer ambiguity processes. The initial ambiguity resolution process applies an iterative partial ambiguity resolution method to obtain an approximate solution. The irrespective of integer ambiguity process transforms the approximate solution to a high-precision solution. Experiments show that the approximate solution is unreliable when the initial ambiguity resolution process has small redundancy, and the proposed method can obtain better results for those cases. The IIAR method showed about a 40% improvement of multi-GNSS ambiguity success rate and about a 25% improvement of standard deviation. Therefore, these results show that the proposed IIAR method can improve the results of multi-GNSS RTK positioning significantly.

Archaeologists have long recognized that precise three-dimensional coordinates are critical for recording objects and features across sites and landscapes. Traditionally, for relatively small areas, an optical transit or, more recently, an electronic distance measurement device (EDM) has been used to acquire these three-dimensional points. While effective, such systems have significant limitations in that they require a clear line of site. Real-time kinematic (RTK) GPS/GNSS systems (Global Positioning System/Global Navigation Satellite Systems) have been available for well over a decade, and can provide quick and accurate point measurements over a wide area without many of the limitation of older technologies. The cost of such systems, however, has generally been prohibitive for archaeologists, and so their use has been rare. Recently, a new generation of low-cost systems has become available, making this technology more accessible to a wider user base. This article describes the use, accuracy, and limitations of one such low-cost system, the Emlid Reach RS, to show why this is an important tool for archaeological fieldwork.

This paper proposes a model for combined Global Positioning System (GPS) and BeiDou Navigation Satellite System (BDS) Real-Time Kinematic (RTK) positioning. The approach uses only one common reference ambiguity, for example, that of GPS L1, and estimates the pseudo-range and carrier phase system and frequency biases. The validations show that these biases are stable during a continuous reference ambiguity period and can be easily estimated, and the other estimated double-differenced ambiguities, such as those of GPS L2, BDS L1, and BDS L2, are not affected. Therefore, our approach solves the problems of a frequently changing reference satellite. In addition, because all the carrier phase observations use the same reference ambiguity, a relationship is established between the different systems and frequencies, and the strength of the combined model is thus increased.

Centimetre-level RTK solutions are mainly influenced by satellite orbit errors, ionospheric and tropospheric delays, and measurement noise (including multipath effects). Estimation and mitigation of the main errors for the CM-level Compass RTK solutions over medium-long baselines are investigated. Tests conducted for this research lead to the following conclusions:

1. 1. For 100 km baselines, a 4 cm error in height component will be induced by a 10 m orbit error. For longer baselines, rapid precise ephemeris will be needed for CM-level accuracy RTK solutions.

2. 2. The residual ionospheric delay error can be eliminated using the optimal triple-frequency ionosphere-free linear combination with the coefficients of 2·6087, −0·5175 and −1·0912 respectively for observations on f1, f2 and f3 frequencies. This combination is optimal in terms of its noise level, e.g., the noise is only amplified three times. It can be used for high accuracy RTK positioning.

3. 3. The residual tropospheric delay can be resolved for the introduced relative zenith tropospheric delay (RZTD) parameters.

Ultra-wideband (UWB) ranging radios, an emerging technology that offers precise, short distance range measurements are investigated as a method to augment carrier-phase GPS positioning. A commercially available UWB ranging system is used in a tightly-coupled GPS and UWB real-time kinematic (RTK) system. The performance of the tightly-coupled system is evaluated in static and kinematic testing. This work demonstrates that UWB errors can be successfully estimated in a real-time filter. The results of static testing show that the integrated solution provides better accuracy, better ability to resolve integer ambiguities and enhanced fixed ambiguity solution availability compared with GPS alone. In kinematic testing in a degraded GPS environment, sub-decimetre accuracy was maintained.