We report new AMS radiocarbon dates of 16 samples from the Holocene deposits of the Vistula Spit, a large coastal barrier landform on the Southern Baltic coast. Collection of the samples was conducted directly from the sedimentary succession excavated during 2020–2022 construction of the Vistula Spit shipping canal. The dated material represents several paleosol horizons and peat lenses buried in the dune deposits, as well as their substrate – beach and shallow marine deposits.