Introduction. Traditionally the control of fruit flies is based on the useof pesticides, which increase production costs and have led to many environmentalconcerns. Our study aimed to evaluate the effect of neem cake on the larval and pupalstages of Ceratitis capitata. Materials and methods. Thirdinstar larvae were transferred to plastic vials with neem cake in different proportions.The treatments consisted of vermiculite with neem cake in the concentrations: control(vermiculite only), then (10, 25, 50, 75 and 100)% of neem seed cake. The bioassay wascompletely randomized, with six treatments, ten replicates and ten third instar larvae perreplicate. The results were statistically analyzed. Results. Atconcentrations of 75% or more of neem cake in vermiculite, practically no adult fliesemerged. At 50%, more than 80% of the adults did not emerge and the pupal period wasprolonged. The sex ratio of the adults was not affected after the contact of the pupaewith the neem cake. The estimated lethal concentrations to kill 50% and 90% of thepopulation were 30.6% and 51.6%, respectively. Conclusion. The neem seed cakehas contact actions against the larval-pupal stage of C.capitata. Neem seed cake in the pupation substrate can cause highmortality when its concentration is 50% or more, and it prolongs the delay of the pupalperiod of C. capitata.