Light and temperature conditions trigger germination in specific temporal windows and microhabitats, thus determining the germination niche of plant species. Manihot species grow in fire-prone ecosystems and their seeds show heat tolerance. Successful establishment in disturbed environments might be related to seed attributes that allow seeds to cope with or avoid stressful environments. We studied some characteristics of the germination niche of Manihot grahamii, a pioneer woody species present in dry subtropical forests of central Argentina, to understand its successful establishment in disturbed environments. We evaluated the germination ecology of the seeds of M. grahamii with the aims to (1) characterize seed traits (viability, mass and moisture content); (2) determine whether it has dormancy and if it is physical or physiological; (3) evaluate the effect of several pre-treatments (gibberellic acid, after ripening, dry prechilling and dry prechilling + warm) on seed dormancy; and (4) assess the effect of different environmental events of high temperatures on the germination process simulating two treatments: fire intensities (with three levels of heat shock) and a gap temperature. M. grahamii seeds have large mass (0.24 g), low moisture content (8%), physiological dormancy, negative photoblastic behaviour and high heat tolerance. Dormancy was alleviated and seeds became insensitive to light when they were exposed to pre-treatments of dry prechilling + warm and high-temperature treatments. This germination strategy promotes secure germination timing into the rainy season on undisturbed habitats as well as a cue for competition-released gaps which in turn favour recruitment in open, disturbed and dry habitats, respectively. In the context of global change, with an increasing habitat fragmentation and fire frequency, M. grahamii could become more abundant and extend its geographic distributional range in central Argentina.

Weed species composition and density were recorded in three identical field experiments established 26 to 30 yr ago in southern Sweden. Each experiment compared three 6-yr crop rotations and four rates of nitrogen application. The rotations differed by having (1) a 2-yr rotational grassland, (2) a 2-yr mixed rotational grassland (legume/grass), or (3) spring wheat followed by fallow. Other crops in the rotations were winter turnip rape, winter wheat, spring oats, and spring barley. Using multivariate analyses, the relative importance of site, crop, crop rotation, and nitrogen application rate on the weed flora was determined. The greatest difference was found between sites, and the second most important factor was crop species. Nitrogen application rate weakly influenced the weed flora, while differences between crop rotations were hardly detectable.

Giant ragweed germination is delayed by both a physiological dormancy of theembryo (embryo dormancy) and an inhibitory influence of embryo-coveringstructures (covering structure-enforced [CSE] dormancy). To clarify theroles of embryo and CSE dormancy in giant ragweed seedling emergence timing,we conducted two experiments to address the following objectives: (1)determine changes in germinability for giant ragweed dispersal units(hereafter “involucres”) and their components under natural burialconditions, and (2) compare embryo and CSE dormancy alleviation andemergence periodicity between successional and agricultural populations. InExperiment 1, involucres were buried in crop fields at Columbus, OH,periodically excavated, and brought to the laboratory for dissection.Involucres, achenes, and embryos were then subjected to germination assaysat 20 C. In Experiment 2, temporal patterns of seedling emergence weredetermined at a common burial site. Reductions in embryo and CSE dormancywere compared with controlled-environment stratification followed bygermination assays at 12 and 20 C, temperatures representative of soilconditions in spring and summer. Results indicated that overwinter dormancyloss involved sequential reductions in embryo and CSE dormancy. CSEdormancy, which may limit potential for fatal germination during fall, wascaused by the pericarp and/or embryo-covering structures within thepericarp. In Experiment 2, successional populations emerged synchronously inearly spring, whereas agricultural populations emerged throughout thegrowing season. Levels of embryo dormancy were greater in the agriculturalpopulations than the successional populations, but CSE dormancy levels weresimilar among populations. In 12 C germination assays, embryo dormancylevels were positively correlated with time required to reach 95% cumulativeemergence (run 1: r = 0.81, P = 0.03; run 2: r = 0.76, P = 0.05). These results suggest thatlate-season emergence in giant ragweed involves high levels of embryodormancy that prevent germination at low temperatures in spring.

Horseweed is a surface-germinating ruderal facultative winter annual. The ruderal nature is a key adaptive characteristic that implicates emergence timing as an important recruitment factor. Experiments were established at three sites in southern Ontario, Canada, from 2009 to 2012 to determine the possible effect of emergence timing of horseweed on plant number, fecundity, and flowering timing. Emerged seedlings were tagged in 0.25-m2 plots in five 2-wk cohorts in the fall and spring of each experimental season. Each plot was followed though until the plants contained within each plot completed their life cycle. Generally, spring-emerging plants were found to flower earlier than fall-emerging plants, but with fall emergence there were higher plant densities in August each season compared with spring emergence. Overall, there was no difference in fecundity between spring- or fall-emerging cohorts, but when cohorts were parsed beyond just spring or fall emergence, we found that plants emerging in early fall and early spring were more fecund and flowered earlier than plants emerging in late fall and late spring. Disturbance (tilled versus not-tilled) significantly affected emergence levels but not emergence timing. The differences in performance among emergence cohorts are likely due to spatial or temporal density-dependent growth advantages. These results show that spring-emerging cohorts of horseweed, especially early spring–emerging cohorts, should not be discounted when considering the weediness of this species, and this may hold true for other facultative winter annual weeds as well.

Many studies have claimed that fire acts as the chief ecological factor cueing dormancy break in seeds with a water-impermeable seed coat, i.e. physical dormancy (PY), in Mediterranean ecosystems. However, a proposal is made that seasonal temperature changes must be viewed as more meaningful dormancy-breaking cues because: (1) fire is erratic and may break PY in seasons during which seedlings cannot complete their life cycle; (2) fire may not occur for long periods, thereby only providing an opportunity for dormancy break and germination once in every several years; and (3) if fire opens the specialized anatomical structures called ‘water gaps’, in seconds, their evolutionary role of detecting environmental conditions becomes irrational. Although fire breaks dormancy in a proportion of seeds, given the risk of seed mortality and the post-fire environment providing cues for dormancy break, it is suggested that fire might possibly be an exaptation.

Temperature is a significant factor influencing seed germination and for many species temperature-mediated germination cues are vital for plant persistence. Rising temperatures forecast as a result of anthropogenic climate change may have a substantial influence on the population and range dynamics of plant species. Here, we report on the thermal constraints on seed germination in natural populations of four congeneric Banksia species collected from a longitudinal climate gradient in Western Australia. We investigated whether germination niche: (1) varied between species; (2) varied among populations of each species; and (3) varied in a consistent manner reflecting the climatic gradients of seed origin. We hypothesized that species would differ and that populations from warmer sites would have a broader temperature window for germination than populations from cooler sites. Species differed in the breadth of their germination niche, but temperatures that stimulated the most rapid and complete germination were similar across all species. A sharp reduction in germination percentage occurred above the optimum temperature, which coincided with significant delays in germination relative to the optimum. The temperatures causing these declines varied among populations. Across the species, there was a significant correlation between optimum germination temperature and mean annual temperature at seed source; however, there was no relationship at the population level for individual species. These data provide insight into the vulnerability of Banksia species to climate change, with those populations that require lower temperatures for germination, or have narrower optimal ranges for germination, likely to be most vulnerable to a warming climate.

Timing of seed germination influences plant lifetime fitness and can affect the ability of plant populations to colonize and persist in changing environments. However, the genetic variation of the seed germination response remains poorly understood. The amplified restriction fragment polymorphism (AFLP) technique was applied to characterize the genetic variation of germinated seeds collected from three Festuca hallii populations in the Canadian prairie. Three subpopulations with early, intermediate and late germination were identified from each population, based on germination tests at 10, 15 and 20°C in controlled growth chambers. Three AFLP primer pairs were employed to screen a total of 540 assayed seedling samples and 188 polymorphic AFLP bands were scored for each sample. None of the assayed AFLP bands were significantly associated with seed germination, but marked differences in estimates of mean band frequency were observed for various groups of germinating seeds under different test temperatures. Partitioning of the total AFLP variation showed that 5.9% AFLP variation was present among seeds of the three populations, 0.3% among seeds of three germination subpopulations, and 0.5% among seeds grouped for germination temperature. Genetic differentiation was significant among 27 groups of seeds representing population, germination timing and test temperature. Subpopulations with early and intermediate germination shared similar genetic backgrounds and were genetically differentiated from the late germination subpopulation. These results indicate that seed genotypes respond slightly differently to environmental variation, resulting in significant but weak genetic differentiation in the germination of F. hallii seeds. Implications for plant establishment and fescue restoration are discussed.