Kochia is a troublesome weed throughout the western United States. Althoughglyphosate effectively controls kochia, poor control was observed in severalno-till fields in Kansas. The objectives of this research were to evaluatekochia populations response to glyphosate and examine the mechanism thatcauses differential response to glyphosate. Glyphosate was applied at 0, 54,109, 218, 435, 870, 1305, 1740, 3480, and 5220 g ae ha−1 on 10kochia populations. In general, kochia populations differed in theirresponse to glyphosate. At 21 d after treatment, injury from glyphosateapplied at 870 g ha−1 range from 4 to 91%. In addition,glyphosate rate required to cause 50% visible injury (GR50)ranged from 470 to 2149 g ha−1. Differences in glyphosateabsorption and translocation and kochia mineral content were not sufficientto explain differential kochia response to glyphosate.