β-Galactosidases from Kluyveromyces lactis and Kluyveromyces marxianus isolated from artisanal ewes’ milk cheeses, were used to transgalactosylate lactose from cheese whey permeate (WP). The content of galactooligosaccharides (GOS) obtained by transgalactosylation was comparable with that formed using pure lactose as substrate. In order to obtain a mixture with higher prebiotic oligosaccharide content, isomerisation of the transgalactosylated WP was carried out using sodium aluminate as catalyst. The transgalactosylated mixtures at 6 h of reaction contained amounts of prebiotic carbohydrates (tagatose, lactulose, GOS and oligosaccharides derived from lactulose, OsLu) close to 50 g/100 g of total carbohydrates for all the strains tested, corresponding to 322 g prebiotics/kg whey permeate. Thus, the suitability of this methodology to produce mixtures of dietary non-digestible carbohydrates with prebiotic properties from WP has been demonstrated, which is interesting for the food industry since it increases the value and the applicability of this by-product from cheese manufacture.

Introduction. Food processing significantly lowers the quality of fruits andvegetables, which is a major concern for the food industry. Micronutrients areparticularly affected, and among them β-carotene, which exhibits veryinteresting sensory, nutritional and biological properties. The literature concerningβ-carotene degradation is extensive, but the conclusions are verydifferent as a function of the biological, chemical and food transformation points ofview. This paper proposes a synthesis of complementary approaches in the study ofβ-carotene during food transformation and storage. Degradationreactions. Degradation compounds are numerous, including isomers, epoxides,apocarotenones, apocarotenals and short-chain cleavage products, among them some flavourcompounds. A detailed reaction scheme of isomerisation and autoxidation ofβ-carotene could be deduced from the literature data. The main pathwaysare well documented, but the global reaction scheme is still incomplete. Furthermore, mostof the mechanistic studies are carried out in model systems, thus data may misrepresentβ-carotene behaviour in real food products. Kinetics duringprocessing and storage The determination of degradation kinetics permits theidentification of the fastest reactions,i.e., generallythose with the greatest impact, and also the quantification of the effect of the factorswhich can lower β-carotene content. Temperature, occurrence of oxygen,food composition and food structure are shown to affect the β-caroteneloss rate significantly. However, the methodologies used to obtain the kinetic parametersare of major importance, and finally, most of the results found in the literature arespecific to a study and difficult to generalise. Discussion and conclusion.Mechanistic and kinetic approaches each provide interesting data to improve understandingand monitoring of β-carotene. The combination of all this data, togetherwith thermodynamic and analytical considerations, permits the building of observablereaction schemes which can further be transcribed through mathematical models. By thismultidisciplinary approach, scarcely used for the time being, knowledge could becapitalised and useful tools could be developed to improve β-caroteneretention during food processing and storage.

Dietary lycopene consists mostly of the (all-E) isomer. Upon absorption, (all-E) lycopene undergoes isomerisation into various (Z)-isomers. Because these isomers offer potentially better health benefits than the (all-E) isomer, the aim of the present study was to investigate if the profile of lycopene isomers in intestinal lipoproteins is affected by the profile of lycopene isomers in the meal and by the tomato preparation. Six postprandial, crossover tests were performed in healthy men. Three meals provided about 70 % of the lycopene as (Z)-isomers, either mainly as 5-(Z) or 13-(Z), or as a mixture of 9-(Z) and 13-(Z) lycopene, while three tomato preparations provided lycopene mainly as the (all-E) isomer. Consumption of the 5-(Z) lycopene-rich meal led to a high (60 %) proportion of this isomer in TAG-rich lipoproteins (TRL), indicating a good absorption and/or a low intestinal conversion of this isomer. By contrast, consumption of meals rich in 9-(Z) and 13-(Z) lycopene isomers resulted in a low level of these isomers but high amounts of the 5-(Z) and (all-E) isomers in TRL. This indicates that the 9-(Z) and 13-(Z) isomers were less absorbed or were converted into 5-(Z) and (all-E) isomers. Dietary (Z)-lycopene isomers were, therefore, differently isomerised and released in TRL during their intestinal absorption in men. Consuming the three meals rich in (all-E) lycopene resulted in similar proportions of lycopene isomers in TRL: 60 % (all-E), 20 % 5-(Z), 9 % 13-(Z), 2 % 9-(Z) and 9 % unidentified (Z)-isomers. These results show that the tomato preparation has no impact on the lycopene isomerisation occurring during absorption in humans.

Lycopene in fruits and vegetables occurs mostly (80–97 %) in the all-E configuration, whereas a considerable proportion of lycopene in the human body is present as Z-isomers. The Z-isomers offer potentially better health benefits and show improved antioxidant activity in vitro when compared with the all-E-isomer. The absorption of dietary lycopene is a complex process involving transfer of the carotenoid from the food matrix into micelles, uptake by enterocytes, packaging into chylomicrons and finally secretion into plasma. Isomerisation could take place at any of these individual steps. By exploiting in vitro and in vivo models, we traced lycopene isomerisation during absorption using various methods to mimic gastric and duodenal conditions, incorporation into mixed micelles, absorption and metabolism by various Caco-2 cell clones, and performed a postprandial study in human subjects to identify the profile of lycopene isomers in plasma chylomicrons. We demonstrate that all-E-lycopene remains unchanged during its passage in the gastrointestinal tract, including its incorporation into mixed micelles. The key site of lycopene isomerisation is inside the intestinal cells resulting in 29 % of lycopene as Z-isomers. Lycopene isomerisation in the various Caco-2 cell clones is consistent with that observed in human chylomicrons formed in a postprandial state. There is no selection in the release of lycopene isomers from enterocytes. Although there is a huge inter-individual variability of total lycopene absorption reported both in in vitro intestinal cell lines as well as in human chylomicrons, the lycopene isomer profile is quite similar.