Excavations at the Iron Age site of Worlebury hillfort during the mid-late 19th century revealed a large number of human skeletal remains, interpreted as victims of a ‘massacre’. Reanalysis of these remains, combining AMS dating, osteological, aDNA, histotaphonomy, and isotope analysis, has enabled a re-evaluation of this hypothesis. AMS dating lends support to the notion that many of these individuals may have died during a single episode, while osteological analysis has identified significant evidence for perimortem trauma, and the histology supports a short period between death and deposition. The genetic data suggest that the human remains represent a group with biological links through the maternal line and connections to another nearby site, while the isotope values are consistent with a local population, consuming animals raised in a salt-marsh environment like the Severn Estuary. Our results demonstrate the value of returning to often unpromising antiquarian collections using an integrated suite of modern analytical approaches.