Over the past 50 years, attention has intensified on Biological and Toxin Weapons Convention (BWC) Article X, which obligates states parties to provide international cooperation and assistance (ICA) and ensure the broadest accessibility of biology for peaceful purposes. In the absence of a treaty protocol and institutional support, Article X’s scope of activities and standard for compliance remain up to the interpretation of each state party. The Ninth BWC Review Conference established the Working Group on the Strengthening of the Convention (Working Group) and mandated it to address ICA, including establishing a mechanism to facilitate Article X implementation. Utilizing a mixed-methods methodology, this study characterizes the landscape of Article X and ICA perspectives among BWC delegations and other stakeholders. It identifies concrete opportunities to strengthen Article X implementation, in support of the Working Group’s efforts, including a pillar framework to illustrate alignment across an ICA mechanism’s roles, activities, and resources.